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Viral Genetics
Viral Genetics

... While the genomes of DNA viruses are relatively stable, the high rates of spontaneous mutation in RNA genomes suggest that the concept of wild type is very fleeting in RNA ...
DEPARTMENT OF MICROBIOLOGY University of Delhi South campus New Delhi-110021 PhD Course work
DEPARTMENT OF MICROBIOLOGY University of Delhi South campus New Delhi-110021 PhD Course work

... Construction of genomic and cDNA libraries: Vectors used in the construction of cDNA versus genomic DNA libraries. Library construction.Library screening by colony hybridization and colony PCR.Screening of expression libraries.Use of subtractive hybridization and positive selection in enrichment. ...
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Challenges and Opportunities in Plant Biotechnology Food

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analysis of gene function
analysis of gene function

...  Introduction of targeting vector into ES cell with the ES cell, a specific gene is substituted by homogenous recombination of targeting vector with the knockouting gene.  Injecting the ES cell into blastodermic vesicle the ES cells of gene knockout form cell clump with the normal cell in blastod ...
one length from each parent
one length from each parent

... Allele that may mask the presence of another ...
Questions11.february
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... Most genes are transcribed into t-RNAs mRNAs ribosomal RNAs repeat elements small nuclear RNAs ...
Lecture 1: Molecular Biology
Lecture 1: Molecular Biology

... primer base-paired to the template strand, a DNA polymerase adds nucleotides to the free hydroxyl group at the 3’ end of the primer. • DNA replication requires assembly of many proteins (at least 30) at a growing replication fork: helicases to unwind, primases to prime, ligases to ligate (join), top ...
Chapter 14 The Human Genome
Chapter 14 The Human Genome

... development As we learned in Chapter 11, some genes are linked-they are located on the same ...
Materials and Methods - Philosophical Transactions of the Royal
Materials and Methods - Philosophical Transactions of the Royal

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Voting: In Your Genes? - James Fowler
Voting: In Your Genes? - James Fowler

... records. All twins were same-sex pairs to avoid confounding results with sex differences. The researchers corrected for environmental factors such as whether more of the identical than fraternal twins were living together, which might inflate their degree of similarity. The researchers concluded tha ...
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Study Guide for Evolution and Genetics Final Exam

... Sexual Selection, Genetic Drift, Gene Flow, Mutation)? Define each and give an example for each. Be able to identify which mechanism caused the evolution of a certain trait. 36. What is the only mechanism for evolution that creates new traits? 37. If a parent gets a tan, do they pass their tan onto ...
GoFigure: Automated Gene Ontology annotation
GoFigure: Automated Gene Ontology annotation

... or multiple DNA or protein sequences. The user chooses one or more ontology for GoFigure to display and supplies an email address for return of results. In the resulting graphs, colored boxes indicate term hits, with darker color indicating lower E-value. Colored boxes can be clicked on, and will br ...
Sunday, 28 October 2007
Sunday, 28 October 2007

... technology, specific genetic pathways which have been disrupted. Heads from E18 homozygous MEKK4 knockout and homozygous normal fetuses from the same mating were collected. Total cellular RNA was extracted and matched pairs of homozygous knockout and normal samples were applied to the Affymetrix mou ...
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...  Developed a genetic screening system to isolate termination defective mutants from S. pombe.  Prepared random mutagenic library of various subunits of RNA polymerase III and developed strains containing mutant’s subunits.  Isolated transcription defective mutants from five different subunits of ...
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103 Lecture Ch22a

... synthesized, the ribosome reaches the the “stop” codon: UGA, UAA, or UAG • There is no tRNA with an anticodon for the “stop” codons • Therefore, protein synthesis ends (termination) • The polypeptide is released from the ribosome and the protein can take on it’s 3-D structure (some proteins begin fo ...
Cytogenetics to Cytogenomics: An Introduction to Genomic
Cytogenetics to Cytogenomics: An Introduction to Genomic

... To understand the role that chromosomal variations play in both constitutional disorders and cancer, cytogenetic analysis is an integral part of current genomic medicine. Chromosomal abnormalities, including aneuploidies, deletions, duplications, and rearrangements, may result in misregulation of ge ...
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Gene therapy for Leber congenital amaurosis

... RPE65 the first eye gene therapy trial? • The genetic cause of the disease is well understood • Although vision is affected early in life and worsens over time, photoreceptor cells remain healthy in the early stages of disease so they can be targeted by gene therapy vectors • We have established pro ...
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...  Exception: Genes that have loci very close to one another on a chromosome tend to stay together during meiosis ...
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Practical Issues in Microarray Data Analysis

... stabilize variance  Durbin and Huber derived variance-stabilizing transform ...
Lecture PPT - Carol Lee Lab
Lecture PPT - Carol Lee Lab

... Gene Transfer - the lateral transmission of genes between individual cells, either directly or indirectly. This transfer of genes between organisms occurs in a manner distinct from the vertical transmission of genes from parent to offspring via sexual reproduction, which could include transformation ...
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... Mutations do not affect the fitness of an organism to the same degree. Natural selection causes many to be lost from the gene pool and the changes that remain are referred to as substitutions. Substitution rates can be used to measure the functional importance of a gene. ...
Genetic Variation: Horizontal Gene Transfer
Genetic Variation: Horizontal Gene Transfer

... Horizontal Gene Transfer Across Domains of Life • Analysis of 40 animal genomes (e.g. Drosophila, C. elegans, primates) found that all the animal genomes acquired ~10s-100s active genes via HGT • In the case of humans, 145 genes seemed to be acquired from other species, most from bacteria and p ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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