Abstract Here we describe our unprecedented approach in
... 2.7.1. DNA microarray preparation Total hepatic and colonic RNA from each respective group were pooled (n = 7). The microarray analysis was carried as out as described previously in Jia et al. (2013). Mouse Genome 430 2.0 Array GeneChips (Affymetrix, Santa Clara, CA, USA) containing over 30,000 gene ...
... 2.7.1. DNA microarray preparation Total hepatic and colonic RNA from each respective group were pooled (n = 7). The microarray analysis was carried as out as described previously in Jia et al. (2013). Mouse Genome 430 2.0 Array GeneChips (Affymetrix, Santa Clara, CA, USA) containing over 30,000 gene ...
On the heredity trail
... discusses the potential for gene modification with tools such as CRISPR–Cas9. This section concludes with some of what Mukherjee does best, combining stories of real patients with the ethical dilemmas raised by their conditions — in this case, what would happen if their disorders were the subject of ...
... discusses the potential for gene modification with tools such as CRISPR–Cas9. This section concludes with some of what Mukherjee does best, combining stories of real patients with the ethical dilemmas raised by their conditions — in this case, what would happen if their disorders were the subject of ...
Prof. Kamakaka`s Lecture 11 Notes
... So what we would like to know is where are the genes located on the chromosomes What does this mean in terms of chromosomes and DNA? ...
... So what we would like to know is where are the genes located on the chromosomes What does this mean in terms of chromosomes and DNA? ...
HL DNA_Jeopardy 2016
... It is not part of the bacteria pellet, because Sulfur-34 would be part of the protein coat of the bacteriophage/ virus which is not injected into the bacteria during infection. ...
... It is not part of the bacteria pellet, because Sulfur-34 would be part of the protein coat of the bacteriophage/ virus which is not injected into the bacteria during infection. ...
annotation transcriptomics doc
... Theory The Gene Ontology provides a structured and controlled vocabulary for the annotation of gene products. Using Gene Ontology database terms allows you to add some semantics to data, and to standardize their annotation. Thus it becomes easier to query the function of gene products. Blast2GO is a ...
... Theory The Gene Ontology provides a structured and controlled vocabulary for the annotation of gene products. Using Gene Ontology database terms allows you to add some semantics to data, and to standardize their annotation. Thus it becomes easier to query the function of gene products. Blast2GO is a ...
PPT
... Can be considered as frequency of occurence X expressive power of regulatory motif It is squared to get rid of negatives Correlate gene expression with occurence of motif Largest dot product is most significant motif ...
... Can be considered as frequency of occurence X expressive power of regulatory motif It is squared to get rid of negatives Correlate gene expression with occurence of motif Largest dot product is most significant motif ...
Genetic Inheritance
... • Law of segregation: reproductive cells carry only one copy of each gene • Law of independent assortment: genes for different traits are separated from each other independently during meiosis; applies in most cases Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings. ...
... • Law of segregation: reproductive cells carry only one copy of each gene • Law of independent assortment: genes for different traits are separated from each other independently during meiosis; applies in most cases Copyright © 2003 Pearson Education, Inc. publishing as Benjamin Cummings. ...
Evolution of the defensin-like gene family in grass genomes
... Plant defensins are small, diverse, cysteine-rich peptides, belonging to a group of pathogenesis-related defense mechanism proteins, which can provide a barrier against a broad range of pathogens. In this study, 51 defensin-like (DEFL) genes in Gramineae, including brachypodium, rice, maize and sorg ...
... Plant defensins are small, diverse, cysteine-rich peptides, belonging to a group of pathogenesis-related defense mechanism proteins, which can provide a barrier against a broad range of pathogens. In this study, 51 defensin-like (DEFL) genes in Gramineae, including brachypodium, rice, maize and sorg ...
Heritable genome-wide variation of gene expression and promoter methylation between
... of either inheritance of the epigenetic changes independently of genetic changes, or result from sequence differences which secondarily affect methylation at close or remote loci. This is more difficult to differentiate, since it would require extensive resequencing data of the individuals actually ...
... of either inheritance of the epigenetic changes independently of genetic changes, or result from sequence differences which secondarily affect methylation at close or remote loci. This is more difficult to differentiate, since it would require extensive resequencing data of the individuals actually ...
Evolutionary steps of sex chromosomes reflected in
... It has been shown that selective pressure to compensate for the silencing of the sex chromosomes during male meiosis resulted in many X-linked genes being duplicated as functional retrogenes on autosomes. Sex chromosome silencing in males was probably stratified during evolution, in accordance with ...
... It has been shown that selective pressure to compensate for the silencing of the sex chromosomes during male meiosis resulted in many X-linked genes being duplicated as functional retrogenes on autosomes. Sex chromosome silencing in males was probably stratified during evolution, in accordance with ...
A Set of Tools Developed for the Analysis of Open Reading Frames
... The DNA sequence of chromosome VI (270kb) of the budding yeast S. cerevisiae, has been determined. The sequence data were then analyzed with the software GENETYX (Software Development Co.) to nd out the candidates of the coding region. As there is little number of intron in budding yeast genome, it ...
... The DNA sequence of chromosome VI (270kb) of the budding yeast S. cerevisiae, has been determined. The sequence data were then analyzed with the software GENETYX (Software Development Co.) to nd out the candidates of the coding region. As there is little number of intron in budding yeast genome, it ...
Novel data clustering for microarrays and image segmentation
... Either way, the absolute or comparison gene expression values are stored in a *.chp file, which serves as the input for high-level statistical analysis. Typically, multiple GeneChip tests are performed giving multiple *.chp files with gene expression values. ...
... Either way, the absolute or comparison gene expression values are stored in a *.chp file, which serves as the input for high-level statistical analysis. Typically, multiple GeneChip tests are performed giving multiple *.chp files with gene expression values. ...
Molecular Cloning
... Process by which a plasmid is used to import recombinant DNA into a host cell for cloning. In DNA cloning, a DNA fragment that contains a gene of interest is inserted into a cloning vector or plasmid. The plasmid carrying genes for antibiotic resistance, and a DNA strand, which contains the gene of ...
... Process by which a plasmid is used to import recombinant DNA into a host cell for cloning. In DNA cloning, a DNA fragment that contains a gene of interest is inserted into a cloning vector or plasmid. The plasmid carrying genes for antibiotic resistance, and a DNA strand, which contains the gene of ...
Mutations in gamma adducin lead to an inherited
... Those who do have abnormal MRIs often have nonspecific findings “These findings suggest unknown pathophysiologic processes” ...
... Those who do have abnormal MRIs often have nonspecific findings “These findings suggest unknown pathophysiologic processes” ...
Gene Section EXT1 (exostoses (multiple) 1) Atlas of Genetics and Cytogenetics
... karyotypic abnormalities of the hereditary multiple exostoses chromosomal loci 8q24.1 (EXT1) and 11p11-12 (EXT2) in patients with sporadic and hereditary osteochondromas. Cancer. 1998 May 1;82(9):1657-63 ...
... karyotypic abnormalities of the hereditary multiple exostoses chromosomal loci 8q24.1 (EXT1) and 11p11-12 (EXT2) in patients with sporadic and hereditary osteochondromas. Cancer. 1998 May 1;82(9):1657-63 ...
Control of female gamete formation by a small RNA pathway in
... Large-scale transcriptional analysis indicated that a gene encoding an ARGONAUTE (AGO) protein (At5g21150 or ARGONAUTE 9) is highly expressed in ovules and anthers of Arabidopsis (Supplementary Fig. 1). The in situ pattern of expression confirmed that AGO9 messenger RNA is localized in ovules throug ...
... Large-scale transcriptional analysis indicated that a gene encoding an ARGONAUTE (AGO) protein (At5g21150 or ARGONAUTE 9) is highly expressed in ovules and anthers of Arabidopsis (Supplementary Fig. 1). The in situ pattern of expression confirmed that AGO9 messenger RNA is localized in ovules throug ...
TWINS AND GENETICS
... SNP at each locus is shown in blue; the correlations (r2) of each of the surrounding SNPs to the sentinel SNP are shown in the colors indicated in the key. The fine-scale recombination rate is shown in blue. Gene positions are indicated at the bottom. ...
... SNP at each locus is shown in blue; the correlations (r2) of each of the surrounding SNPs to the sentinel SNP are shown in the colors indicated in the key. The fine-scale recombination rate is shown in blue. Gene positions are indicated at the bottom. ...
Functional Genomics
... gene for GAL4 is divided between plasmids gene for protein of interest is spliced next to DNA binding domain of GAL4 = bait the other protein gene is spliced next to the rest of the GAL4 gene on other plasmid = target both plasmids introduced into cell or cells of organism together and observed for ...
... gene for GAL4 is divided between plasmids gene for protein of interest is spliced next to DNA binding domain of GAL4 = bait the other protein gene is spliced next to the rest of the GAL4 gene on other plasmid = target both plasmids introduced into cell or cells of organism together and observed for ...
Statistical Methods We used modified segregation analysis to fit a
... normal alleles. These assumptions are not critical to the model because carriers in multiple genes are rare. Thus, Gi takes on the following potential risk categories: MLH1 carriers, MSH2 carriers, MSH6 carriers, PMS2 carriers, MUTYH biallelic carriers, MUTYH monoallelic carriers, UNIDENTIFIED_MAJOR ...
... normal alleles. These assumptions are not critical to the model because carriers in multiple genes are rare. Thus, Gi takes on the following potential risk categories: MLH1 carriers, MSH2 carriers, MSH6 carriers, PMS2 carriers, MUTYH biallelic carriers, MUTYH monoallelic carriers, UNIDENTIFIED_MAJOR ...
Comparison of genes among cereals
... all of the genes of a particular species and to understand the redundant and unique functions of each gene. In the absence of a whole-genome sequence, gene discovery must rely on other tools, the most important of which are expressed sequence tag (EST) libraries. ESTs derived from a diverse set of c ...
... all of the genes of a particular species and to understand the redundant and unique functions of each gene. In the absence of a whole-genome sequence, gene discovery must rely on other tools, the most important of which are expressed sequence tag (EST) libraries. ESTs derived from a diverse set of c ...
Table SI. Primers used for creation of the PHAC1co and
... Bogaert et al., 2009a) was added to the 3’ terminus of the codon-optimized PHAC1co sequence. Additionally the up- and downstream regulatory regions of the CYP52M1 gene were added to the 5’ (488 bp) and 3’ (190 bp) side of the PHAC1co sequence respectively. The 5’ UTR region, was extended beyond the ...
... Bogaert et al., 2009a) was added to the 3’ terminus of the codon-optimized PHAC1co sequence. Additionally the up- and downstream regulatory regions of the CYP52M1 gene were added to the 5’ (488 bp) and 3’ (190 bp) side of the PHAC1co sequence respectively. The 5’ UTR region, was extended beyond the ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.