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Supplementary Figures (doc 928K)
Supplementary Figures (doc 928K)

... entities). Stacked percentage distribution of level 0 cluster sizes from the group of all sequences, divided by cluster specificity. The “specificity” of a cluster is determined by the number of sequences that belong to the CLL group or the non-CLL group. If all sequences are non-CLL then the cluste ...
Respiration Worksheet
Respiration Worksheet

... other type of autosomal dominant disorder is dwarfism, in particular, a form called Achondroplasia. Individuals with dwarfism have a defect in bone growth of the long bones, the arms and legs. As a result, the average height for Achondroplasia dwarves is about 4’ tall. Dwarfism is caused by one dom ...
Genetics and Genomics
Genetics and Genomics

Root organization and gene expression patterns
Root organization and gene expression patterns

... These initials, or histogens (Hanstein, 1868) contribute to the initiation of different tissues; for example, in the most common closed type in monocotyledonous plants (e.g. Poaceae) there are three initial layers, the plerome (vascular cylinder), the periblem/dermatogen (cortex and epidermis) and t ...
edn1 - University of Oregon (SPUR)
edn1 - University of Oregon (SPUR)

... •Amenable to genetics: -Easy to take care of -lay in large numbers •Amenable to embryology: -Clear embryos -Develop rapidly •And they’re vertebrates! -Can study skeletal development -Relevant to human development/disease ...
09:45 PATO: An Ontology of Phenotypic Qualities
09:45 PATO: An Ontology of Phenotypic Qualities

...  post-composition  The post-composition methodology takes advantage of the ability to describe phenotypes by describing the particular affected entity (bearer), which could be an anatomical structure, a biological process, a particular function etc. , and the qualities that this entity possess, wh ...
Transvection and pairing of a Drosophila Hox long noncoding RNA
Transvection and pairing of a Drosophila Hox long noncoding RNA

... with translocations or inversions with breakpoints mapping to the fushi tarazu– Antennapedia (ftz-Antp) interval result in mis-regulation of Scr in trans, on the wildtype (wt) chromosome (Southworth and Kennison 2002). Interestingly, these GOF mutations break within a region in the ftz-Antp interval ...
Studying the pathogenesis of renal disease in cystinosis
Studying the pathogenesis of renal disease in cystinosis

... • Discovery of cystinosin in 1998 opened a new chapter of diseases caused by defects of lysosomal transport proteins. • New data indicate that the expression of cystinosin is not restricted to the lysosomal membrane. Ongoing studies will identify new functions and regulators of cystinosin activity  ...
Chip-seq analysis
Chip-seq analysis

... CEAS can be used to annotate your peak and perform a first general analysis looking for chromosomes and genomic features enriched in peak of interest. One very interesting feature is that any kind of genomic feature can be provided for analysis. Here we will also try to test whether ESR1 peaks are b ...
Application of whole genome sequencing to fully characterise
Application of whole genome sequencing to fully characterise

... Preparation Kit for library preparation for the majority of the strains. Following sequencing using the Illumina HiSeq platform, the data obtained from isolates were automatically analysed through an in-house bioinformatics pipeline, Campype, enabling (i) extraction of MLST data, (ii) multi-sequence ...
FACT SHEET 88 8 AUTOSOMAL RECESSIVE INHERITANCE— Traditional patterns of inheritance 1
FACT SHEET 88 8 AUTOSOMAL RECESSIVE INHERITANCE— Traditional patterns of inheritance 1

Regional DNA Hypermethylation at D17S5
Regional DNA Hypermethylation at D17S5

... studies of colon (2) and brain tumors (1), establish that D17S5 hy permethylation is tightly coupled to 17p deletions and p53 gene mu tations in human cancers. Our results in renal cancer strongly suggest that this hypermethylation precedes the other two events. If so, hy was one tumor (Fig. 3) whic ...
Document
Document

... Imegen is a leading designer and manufacturer of kits for clinical genetic testing based on a wide range of molecular technologies. Our portfolio currently includes more than 100 references for kits based on Sanger and NGS sequencing technologies, digital PCR, and real time PCR. Since 1998, we have ...
Divergent Evolution of Duplicate Genes Leads to Genetic
Divergent Evolution of Duplicate Genes Leads to Genetic

... LE12 5RD, UK. *To whom correspondence should be addressed. E-mail: [email protected] ...
The making of the Fittest: Natural Selection and Adaptation
The making of the Fittest: Natural Selection and Adaptation

... • Emphasize that mutations occur at random, whether they occur in protein-coding regions of genes or regulatory switches. Natural selection, however, is not random; mutations that produce organisms that are more likely to survive and reproduce in a particular environment are more likely to become co ...
GENES AND DEVELOPMENTAL PATHWAYS
GENES AND DEVELOPMENTAL PATHWAYS

... such as the case to be described below, can also be profitably exploited to learn more about how genes control developmental pathways. A number of levels of functional integration of the genetic material can now be recognized, with pseudoallelism being but one example (see reviews by Pontecorvo, 195 ...
Provisional PDF
Provisional PDF

... the highest homology (93%) was rpoB. These results indicate that these genes are from a microorganism belonging to the genus C. Hepatoplasma. For the purpose of this study, this microorganism was designated as “C. Hepatoplasma sp.” ...
1 BIOINFORMATICS Bioinformatics, based on National Institutes of
1 BIOINFORMATICS Bioinformatics, based on National Institutes of

... Bioinformatics, based on National Institutes of Health definition, the following: „Research, development, or application of computational tools and approaches for expanding the use of biological, medical, behavioral or health data, including those to acquire, store, organize, archive, analyze, or vi ...
Milestone10
Milestone10

... You should submit these 21 sequences to the Gibbs Motif Sampler web server. These 21 sequences come from the same genome, why do we expect common patterns in these sequences? Since the microarray data suggests that the genes are co-expressed, i.e., they are expressed under the same conditions, the g ...
Detecting prostate cancer
Detecting prostate cancer

... What are the symptoms of prostate cancer? The following symptoms may be caused by problems that are much less serious than prostate cancer. Try not to worry if you develop any of them, but do get them checked out: • needing to pee often, especially at night • difficulty in starting to pee • strainin ...
Antigen Binding and Idiotype Analysis of Antibodies Obtained
Antigen Binding and Idiotype Analysis of Antibodies Obtained

... * Cell lines designated with ( + ) in a given assay produced an antibody that bound within a twofold range of the antibody used to generate the standard curve; (_+) a quantity 20-40-fold lower than the standard curve antibody was obtained at the 1-#g/ml dilution; and ( - ) nothing was ...
Chapter 7 Operons: Fine Control of Bacterial Transcription Bacterial
Chapter 7 Operons: Fine Control of Bacterial Transcription Bacterial

... • Ribosomes stall at tandem tryptophan codons in leader peptide • Stalled ribosome position influences way mRNA folds • Prevents formation of hairpin; hairpin part of transcription termination signal which caused attenuation if lots of trp and translation of leader. ...
Ribosome Display: In Vitro Selection of Protein
Ribosome Display: In Vitro Selection of Protein

... allowing the selection from very large combinatorial libraries. In add ition, the ropid selection cycles require an integra l polymerase cboin re«ction (PCR) step, which can be used for ra ndomization, making this method ideal for directed evolution experiments. The fact that the ribosomal complex u ...
The ancestral role of Brachyury: expression of NemBra1 in the basal
The ancestral role of Brachyury: expression of NemBra1 in the basal

... site of gastrulation (Hand and Uhlinger 1992). Here we present the isolation of the Nematostella Brachyury homolog, NemBra1, and show the first in situ hybridizations in Nematostella. The analysis supports the view of an ancestral function of Brachyury in designating the blastoporal region and the t ...
Screening of a Specific Point Mutation in Tumor Suppressor p53
Screening of a Specific Point Mutation in Tumor Suppressor p53

... in distilled water, it was digested with HaeIII for 1 h at 37 °C. The digested products were loaded onto 2% Seakem agarose (FMC) gel contating 5 f.1g/ml ethidium bromide in 1X TBE buffer. q>X174/RF HaeIII fragments (NEB) were used as size markers. Analysis of DNA ploidity Thin sections (5 f.1m) were ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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