Mycobacterium tuberculosis: Potassium uptake systems of
... (i) the wild-type (WT) MTB strain and (ii) the Trk-deletion mutant strain of MTB. The presence of the Trk system in the organism in (i) may contribute to the decrease in [H+] leading to high vacuolar pH, which will interfere with phagosome maturation. In (ii) the macrophage infected with the Trk-del ...
... (i) the wild-type (WT) MTB strain and (ii) the Trk-deletion mutant strain of MTB. The presence of the Trk system in the organism in (i) may contribute to the decrease in [H+] leading to high vacuolar pH, which will interfere with phagosome maturation. In (ii) the macrophage infected with the Trk-del ...
bioinformatics module ii - Tetrahymena Genome Database
... molecular biologist. A number of computational programs are available that help search through genome databases, design primers for PCR, translate a DNA sequence into an amino acid sequence (and vice versa), identify conserved regions in the amino acid sequence (domains), etc. In this lab, you will ...
... molecular biologist. A number of computational programs are available that help search through genome databases, design primers for PCR, translate a DNA sequence into an amino acid sequence (and vice versa), identify conserved regions in the amino acid sequence (domains), etc. In this lab, you will ...
Ph.D. THESIS Analysis of the chromatin structure of MDR1 gene in
... The development of multidrug resistance in cancer cells is often caused by increased energy-dependent efflux of hydrophobic drugs that can enter the cell through diffusion. The drug efflux is generally mediated by the increased activity of a transporter, encoded by ABCB1/MDR1 (multidrug resistance 1 ...
... The development of multidrug resistance in cancer cells is often caused by increased energy-dependent efflux of hydrophobic drugs that can enter the cell through diffusion. The drug efflux is generally mediated by the increased activity of a transporter, encoded by ABCB1/MDR1 (multidrug resistance 1 ...
Gene Section EXT2 (exostoses (multiple) 2) Atlas of Genetics and Cytogenetics
... is malignant transformation of an osteochondroma (exostosis) into chondrosarcoma, which is estimated to occur in 1-5% of the HME cases. Cytogenetics 11p rearrangement was found in 1 sporadic osteochondroma (exostosis) using cytogenetic analysis; loss of heterozygosity at the EXT2 locus was absent in ...
... is malignant transformation of an osteochondroma (exostosis) into chondrosarcoma, which is estimated to occur in 1-5% of the HME cases. Cytogenetics 11p rearrangement was found in 1 sporadic osteochondroma (exostosis) using cytogenetic analysis; loss of heterozygosity at the EXT2 locus was absent in ...
The evolution of silicon transporters in diatoms
... harvested when growth was no longer exponential. The presumed growth-limiting macronutrient was added back to the remaining culture after harvesting to confirm the identity of the growth-limiting nutrient. Transcriptome reads are accessible in the National Center for Biotechnology Information (NCBI) ...
... harvested when growth was no longer exponential. The presumed growth-limiting macronutrient was added back to the remaining culture after harvesting to confirm the identity of the growth-limiting nutrient. Transcriptome reads are accessible in the National Center for Biotechnology Information (NCBI) ...
Control of ribosome traffic by position-dependent
... stop-codon reflect the ribosome diameter (11 codons). When one C-rate codon is added at the beginning (Fig. 3b), it increases the coverage of the start codon to somewhat. This slightly reduces the initiation rate resulting in lower coverage and fewer collisions between ribosomes. As the Crate codon ...
... stop-codon reflect the ribosome diameter (11 codons). When one C-rate codon is added at the beginning (Fig. 3b), it increases the coverage of the start codon to somewhat. This slightly reduces the initiation rate resulting in lower coverage and fewer collisions between ribosomes. As the Crate codon ...
Pole Region-Dependent Repression of the Drosophila Gap Gene
... which are expressed in the domains expected from the mutant phenotype (Scott and O’Farrell, 1986), the role of gap genes in the segmentation process is less apparent. In comparison to the large gap seen in the amorphic Kf embryos, the expression domain of Kr in wild-type embryos is relatively small. ...
... which are expressed in the domains expected from the mutant phenotype (Scott and O’Farrell, 1986), the role of gap genes in the segmentation process is less apparent. In comparison to the large gap seen in the amorphic Kf embryos, the expression domain of Kr in wild-type embryos is relatively small. ...
Regulation of Gene Expression by Coupling of Alternative Splicing
... deposited in sequence databases. There are a few known examples in which a transcript that should be degraded according to the 50-nucleotide rule is in fact stable and translated to make protein. These include polycistronic transcripts on which translation is reinitiated downstream of a PTC;21-23 ap ...
... deposited in sequence databases. There are a few known examples in which a transcript that should be degraded according to the 50-nucleotide rule is in fact stable and translated to make protein. These include polycistronic transcripts on which translation is reinitiated downstream of a PTC;21-23 ap ...
Drug Development
... • Absorption - Passes GI track into blood stream • Distribution - Gets to target tissue (blood brain barrier) • Metabolism – Not readily metabolized • Excretion – Not readily secreted • Toxicity – Not toxic to other cells or tissues ...
... • Absorption - Passes GI track into blood stream • Distribution - Gets to target tissue (blood brain barrier) • Metabolism – Not readily metabolized • Excretion – Not readily secreted • Toxicity – Not toxic to other cells or tissues ...
Cloning of the Papaya Chromoplast-Specific
... 2007). A nonsense-mediated posttranscriptional gene silencing (i.e. nonsense-mediated mRNA decay) mechanism was suggested, since no cis-acting regulatory sequences or mutations were identified. The Orange (Or) mutation in cauliflower causes the accumulation of b-carotene in normally nonpigmented tis ...
... 2007). A nonsense-mediated posttranscriptional gene silencing (i.e. nonsense-mediated mRNA decay) mechanism was suggested, since no cis-acting regulatory sequences or mutations were identified. The Orange (Or) mutation in cauliflower causes the accumulation of b-carotene in normally nonpigmented tis ...
Positive and Negative Selection on Noncoding
... Keightley 2006; Veyrieras et al. 2008). Additionally, the interpretation of sequences further upstream and downstream from these regions was made more difficult by frequent indel variation, making calling of single nucleotide polymorphisms (SNPs) problematic. In supplementary table S1, Supplementary ...
... Keightley 2006; Veyrieras et al. 2008). Additionally, the interpretation of sequences further upstream and downstream from these regions was made more difficult by frequent indel variation, making calling of single nucleotide polymorphisms (SNPs) problematic. In supplementary table S1, Supplementary ...
Molecular Cloning, Sequencing, and Expression of the Glutamine
... CTGGCAC .....TTGCA-3') identified from enteric organisms and Bradyrhizobium spp. (12). Codon usage. The coding region for the Frankia glnII gene had a G+C content of 68.7%, which agrees with an estimated total G+C content of 68.4 to 72.1% for Frankia strains in general (2). The genetic code permits ...
... CTGGCAC .....TTGCA-3') identified from enteric organisms and Bradyrhizobium spp. (12). Codon usage. The coding region for the Frankia glnII gene had a G+C content of 68.7%, which agrees with an estimated total G+C content of 68.4 to 72.1% for Frankia strains in general (2). The genetic code permits ...
Gene tree reconstruction and orthology analysis based on
... P r[F, G | λ, µ] is called the likelihood and we will in Section 5.1 show how it can be approximated with small error. The posterior distribution thus assigns to a state (G, λ, µ) the probability that, in the gene evolution process: G was the gene tree, λ was the birth rate, and µ was the death rate ...
... P r[F, G | λ, µ] is called the likelihood and we will in Section 5.1 show how it can be approximated with small error. The posterior distribution thus assigns to a state (G, λ, µ) the probability that, in the gene evolution process: G was the gene tree, λ was the birth rate, and µ was the death rate ...
A rule-based kinetic model of RNA polymerase II C
... adopt [3] are simulated stochastically, and thus the approach accounts for low molecule numbers and the discrete states that molecules can adopt. We discuss transcription by RNA polymerase II (RNAPII) as an example of a complex system that can be modelled in detail using a rule-based approach. We pr ...
... adopt [3] are simulated stochastically, and thus the approach accounts for low molecule numbers and the discrete states that molecules can adopt. We discuss transcription by RNA polymerase II (RNAPII) as an example of a complex system that can be modelled in detail using a rule-based approach. We pr ...
Hair Color is a Heritable Trait
... • Ratio (PV/GV): Made larger by diversifying the genetic variance &/or minimizing environmental effects • Sample specific, as with other correlations • Social control reduces heritability; heritability is generally higher under conditions of low social constraint e.g., Differences in disinhibition ( ...
... • Ratio (PV/GV): Made larger by diversifying the genetic variance &/or minimizing environmental effects • Sample specific, as with other correlations • Social control reduces heritability; heritability is generally higher under conditions of low social constraint e.g., Differences in disinhibition ( ...
Imunocytochemistry Detection of M1CA Gene Receptor in Human P
... Brucella invasion, and the bacteria can survive within this naturally hostile intracellular environment (Pizarro-Cerda,1998). Macrophages are significant in transporting Brucella to tissue throughout the host, where they can survive in a variety of cell types (Arenas, 2000). poor diagnosis and lack ...
... Brucella invasion, and the bacteria can survive within this naturally hostile intracellular environment (Pizarro-Cerda,1998). Macrophages are significant in transporting Brucella to tissue throughout the host, where they can survive in a variety of cell types (Arenas, 2000). poor diagnosis and lack ...
Types of variation in DNA-A among isolates of East African cassava
... true for EACMV, the extent of the sequence dissimilarity between type 1 and type 2 isolates in the intergenic region would be expected to be different on either side of ori. Inspection of the intergenic region sequences shows that, to the 5« side of ori, coordinated differences between EACMVMH and - ...
... true for EACMV, the extent of the sequence dissimilarity between type 1 and type 2 isolates in the intergenic region would be expected to be different on either side of ori. Inspection of the intergenic region sequences shows that, to the 5« side of ori, coordinated differences between EACMVMH and - ...
PDF - Journal of Genomics
... The 88.29 million raw paired-end reads of ≥240 bp were imported into CLC Genomics Workbench (v6.5.1, CLC Bio) and trimmed for quality and adapter indexes using the default settings (Ambiguous limit = 2, quality limit = 0.05). The remaining processed reads (87.53 million) were de novo assembled into ...
... The 88.29 million raw paired-end reads of ≥240 bp were imported into CLC Genomics Workbench (v6.5.1, CLC Bio) and trimmed for quality and adapter indexes using the default settings (Ambiguous limit = 2, quality limit = 0.05). The remaining processed reads (87.53 million) were de novo assembled into ...
Etiology : cytogenetics and microdeletions - HAL
... discarded but instead be considered as candidate genes. Indeed, it was recently shown that not only hemizygous genes but also normal-copy neighboring genes can show decreased levels of expression [Merla and others 2006]. Therefore, genes flanking a genomic rearrangement should be considered as poss ...
... discarded but instead be considered as candidate genes. Indeed, it was recently shown that not only hemizygous genes but also normal-copy neighboring genes can show decreased levels of expression [Merla and others 2006]. Therefore, genes flanking a genomic rearrangement should be considered as poss ...
cancer phenotype in selected families are a feature of the inherited
... in four independent families. This out-of-frame deletion is predicted to result in truncation of the BRCA1 protein at codon 1770. Haplotype analysis was performed for three of those families and there was no evidence of a shared haplotype. Thus, the repeated observation of this mutation is most like ...
... in four independent families. This out-of-frame deletion is predicted to result in truncation of the BRCA1 protein at codon 1770. Haplotype analysis was performed for three of those families and there was no evidence of a shared haplotype. Thus, the repeated observation of this mutation is most like ...
Molecular and Structural Characterization of
... (Ak), in addition to CS, to examine whether the expression pattern of each gene is conserved among the two cultivars. In CS, Taglu1a was most highly expressed (67%) in 48-h-old wheat, with Taglu1b expressed at about onehalf this level (32%; Fig. 2B). The ratio was slightly different in a 96-h-old pl ...
... (Ak), in addition to CS, to examine whether the expression pattern of each gene is conserved among the two cultivars. In CS, Taglu1a was most highly expressed (67%) in 48-h-old wheat, with Taglu1b expressed at about onehalf this level (32%; Fig. 2B). The ratio was slightly different in a 96-h-old pl ...
Environmental Pollution
... above-mentioned target genes. This method provided a clone library, and sequencing revealed information on new prokaryotes which grouped mainly next to uncultured microorganisms. However, due to the high diversity of approximately 104 ribotypes (wdifferent bacteria) in soils (Torsvik et al., 1990), ...
... above-mentioned target genes. This method provided a clone library, and sequencing revealed information on new prokaryotes which grouped mainly next to uncultured microorganisms. However, due to the high diversity of approximately 104 ribotypes (wdifferent bacteria) in soils (Torsvik et al., 1990), ...
Developmental Regulation of Genes Encoding Universal Stress
... endodermal epithelium, a highly potent chemical barrier for protection against intruding microbes. Sequences associated with ESTs, SAGE Tags and microarray probes are functional genomic tools appropriate for dissecting gene functions.22 These tools can provide expression data to facilitate forward a ...
... endodermal epithelium, a highly potent chemical barrier for protection against intruding microbes. Sequences associated with ESTs, SAGE Tags and microarray probes are functional genomic tools appropriate for dissecting gene functions.22 These tools can provide expression data to facilitate forward a ...
RNA-Seq
RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.