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4.2 Sources of DNA
4.2 Sources of DNA

... proteins as cells do. Viral DNA is sometimes used as a vector because they can open to insert genes of interest. Some companies are exploring the use of gene therapy to treat diabetes by replacing defective insulin genes in the pancreas Gene therapy is possible treatment for cystic fibrosis and othe ...
R and BioConductor
R and BioConductor

... • Pair-end reads: use analogous fragments per kilobase of transcript per million mapped reads (FPKM) metric ...
Q on Genetic Control of Protein Structure and function – Chapter 5
Q on Genetic Control of Protein Structure and function – Chapter 5

... Draw a diagram of a single DNA nucleotide. Which enzyme turns DNA nucleotides into a polynucleotide? Explain what is meant by “complementary base pairing”. What type of bond holds the two DNA strands together? What are the 2 essential functions of DNA? What are the 2 main types of RNA and what are t ...
Smurfs, Trolls & Elves
Smurfs, Trolls & Elves

... read or write clearly • Frequently, musical, story-telling and social ability enhanced ...
Presentation - Anil Jegga - Cincinnati Children`s Hospital Medical
Presentation - Anil Jegga - Cincinnati Children`s Hospital Medical

... 1. Gene Ontology: GO and NCBI Entrez Gene 2. Mouse Phenotype: MGI (used for the first time for human disease gene prioritization) ...
Structure,Function of RNA
Structure,Function of RNA

... 1. RNA helps in protein synthesis making gene into a trait 2. It takes a copy of DNA molecule out of the nucleus as a form of mRNA & function as a working copy of DNA This is the general notes on The Structure and Function of RNA ...
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Document

... Gene knockout approach = systematically delete different genes and observe the phenotypes (PCR + cloning is one method). ...
LEARNING GOALS - PROTEIN SYNTHESIS Main Idea
LEARNING GOALS - PROTEIN SYNTHESIS Main Idea

... 1. The enzyme RNA-polymerase reads the DNA molecule in the 3’ to 5’ direction and synthesizes complementary mRNA molecules that determine the order of amino acids in the polypeptide. 2. In eukaryotic cells the mRNA transcript undergoes a series of enzymeregulated modifications. Examples include o Ad ...
RIBONUCLEIC ACID (RNA)
RIBONUCLEIC ACID (RNA)

... • Many viruses encode their genetic information using an RNA genome • Some RNA molecules play an active role within cells by catalyzing biological reactions, controlling gene expression, or sensing and communicating responses to cellular signals • One of these active processes is protein synthesis, ...
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... 7.3 Gene Linkage and Mapping • Cross-over frequencies can be converted into map units. – gene A and gene B cross over 6.0 percent of the time – gene B and gene C cross over 12.5 percent of the time – gene A and gene C cross over 18.5 percent of the time ...
7.3 Gene Linkage and Mapping KEY CONCEPT chromosomes.
7.3 Gene Linkage and Mapping KEY CONCEPT chromosomes.

... 7.3 Gene Linkage and Mapping Linkage maps estimate distances between genes. • The closer together two genes are, the more likely they will be inherited together. • Cross-over frequencies are related to distances between genes. • Linkage maps show the relative locations of genes. ...
Gene Expression Changes and Micro RNA Regulation in Embryonic
Gene Expression Changes and Micro RNA Regulation in Embryonic

... • Genes expressed by hESCs are involved with DNA replication, recombination and repair, RNA damage, and repair RNA posttranscriptional modification, cellular growth and proliferation, and cell cycle • ESC express several transcription factors including Oct4, Sox2, and nanog • Genes in several signal ...
9/16
9/16

... •This DNA is ~2 meters long and 2 nm wide. •~3% directly codes for amino acids •~10% is genes •In a single human cell only about 5-10% of genes are expressed at a time. ...
Crash course on Computational Biology for Computer Scientists
Crash course on Computational Biology for Computer Scientists

... index the genome to be able to process the reads quickly We need to take errors and variants into account, but hopefully not too many of them in a single read We should consider text indexes (Suffix trees, suffix arrays and Burrows-Wheeler transform) ...
rna interference
rna interference

... complexes that contain an argonaute-type endoribonuclease (Ago) and some other proteins (scanning 284. old). Thereafter, the two RNA strands are unwound and one of the strands is degraded by argonaute, leaving a singlestranded RNA bound to the argonaute complex. One type of the argonaute complex is ...
Exploring Comprehensive Gene Expression Analysis of
Exploring Comprehensive Gene Expression Analysis of

... La Tullippe et at. used high-throughput gene expression analysis to study prostate cancer metastasis Differentially expressed genes between metastatic and primary tumors were found in: I. Cell Cycle Regulation II. Mitosis III. Signaling IV. DNA Replication Metastatic tumors had higher proliferation ...
Exam II
Exam II

... 4. In Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III, the authors conducted a successful “fishing” experiment and discovered novel genes. They were blessed with certain computational and technological advances not available in the not-to-far distant ...
Transcription and Translation Eukaryotic Cell
Transcription and Translation Eukaryotic Cell

... Eukaryotic Cell- A cell with a membrane-enclosed nucleus and other membrane-enclose organelles (protists, plants, animals, and fungi). Nucleus- The chromosome containing organelle of a eukaryotic cell. DNA is located in the nucleus, transcription and mRNA processing occurs in the Nucleus. Codon- Spe ...
Chapter 12
Chapter 12

... Figure 12.1 One Gene, One Enzyme (Part 2) ...
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Quick Vocabulary Lesson 1 Lesson 2 dominant trait

... codominance occurs when both alleles can be observed in the offspring’s phenotype ...
Name:
Name:

... 13. If a couple has three children in the family (different ages), do any of them look EXACTLY like a parent? Do the children all look identical to each other? Explain why or why not for each situation. (p.267-269 & 394-395) ...
Part 1
Part 1

... content) relative to their total base content can be used as indicator of relatedness, or lack thereof. For example, an organism with G + C content of 50% will not be closely related to an organism whose G + C content is 25% The order of bases along a strand of DNA or RNA is known as the base sequen ...
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... Twist is a bHLH transcription factor that will later turn on fibroblast growth factor (FGF) receptors 3 hours post-fertilization ...
AP Biology: Unit 3B Homework
AP Biology: Unit 3B Homework

... 7. Compare the three domains (Bacteria, Archaea and Eukarya) in the following ways: genome size, number of genes, gene density and noncoding DNA 8. Describe the following types of DNA sequences that are found in the human genomes and their roles: repetitive DNA, pseudogenes, exons, introns and regul ...
Transcription Control in Eukaryotes
Transcription Control in Eukaryotes

... Example: Activation of GAL Genes in Yeast Tightly linked genes GAL1, GAL7, and GAL10 are coordinately activated or repressed by the activity of two genes on different chromosomes. Their protein products are enzymes required for conversion of galactose to glucose-1-phosphate which enters the glycoly ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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