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Plxdc2 Is a Mitogen for Neural Progenitors Suzanne F. C. Miller-Delaney
Plxdc2 Is a Mitogen for Neural Progenitors Suzanne F. C. Miller-Delaney

Green Fluorescent Protein: A Reporter Molecule
Green Fluorescent Protein: A Reporter Molecule

... Gene Regulation ...
It`s All In The Genes - American Maine
It`s All In The Genes - American Maine

... phenotype. Now let’s say both the bull and cow are black, but they are heterozygous black (Bb - they both carry the recessive red gene). Table 3 shows us what will happen. Three of four calves will be born black, but only one is homozygous black (BB); two of four are heterozygous black (carry the re ...
UV-Visible Analysis of Bitternessand Total Carbohydrates in Beer
UV-Visible Analysis of Bitternessand Total Carbohydrates in Beer

... final IBU of the product is a result of both the amount and type of hops used during the brewing process as well as when the hops are added. Additionally, the type and amount of malt used in beers affects the flavor and can alter its apparent bitterness. In order to maintain consistency in quality, ...
Concepts of Biology
Concepts of Biology

... chromosomes or genes had been identified, at a time when meiosis was not well understood. Mendel selected a simple biological system and conducted methodical, quantitative analyses using large sample sizes. Because of Mendel’s work, the fundamental principles of heredity were revealed. We now know t ...
Uria_et_al_2006 _ADH paper
Uria_et_al_2006 _ADH paper

... then digested into fragments. All resulting fragments can be cloned into an appropriate expression ...
Integration of omics data with biochemical reaction
Integration of omics data with biochemical reaction

... redundancy. Hence, in a following study, two lymphoblastic leukemia cells line models are generated, combining metabolomic and transcriptomic data. The models explain distinctive concentration changes in the spent medium of the two cancer cell lines by different utilization of glycolysis and oxidati ...
Methods S1.
Methods S1.

... independent case-control series, genotype combinations were assigned to the categories H, L and 0, determined for series I; that is, we tested the same high- and low-risk genotype combinations in the second population. Statistical significance was tested by permuting case-control status to generate ...
unit-2 genetics of prokaryotes and eukaryotic
unit-2 genetics of prokaryotes and eukaryotic

... complex of the integrase enzyme (Figure 2.6) with a second bacteriophage protein, which is produced by the virus only when its host cell is stressed. If the sites recognized by such a recombination enzyme are flipped, the DNA between them will be inverted rather than excised (Figure 2.7). Many other ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA

... approximately 1200 bp PCR product besides the approximately 1500 bp specific product, making direct sequencing impossible. Sequence analyses gave interesting results. The reported 0.2 % difference between 16S rRNA gene sequences of type strains of R. qingshengii and R. jialingiae was not found, beca ...
GRASP-DNA: A Web Application to Screen Prokaryotic Genomes for
GRASP-DNA: A Web Application to Screen Prokaryotic Genomes for

... using the same matrix. However, the benefits of searches of this nature still remain unexplored. Following the submission of a search query, GRASP-DNA will notify the researcher of the search completion via email referencing a temporary URL that will house the results and persist for approximately t ...
Introduction to Angelfish Genetics
Introduction to Angelfish Genetics

... Platinum appears to be a recessive trait that modifies a gold angelfish to be a striking iridescent white color. As the angelfish matures, it shows a lot of blue iridescence. ...
W0=2, a stable aneuploid derivative of Candida
W0=2, a stable aneuploid derivative of Candida

... unequal crossing over in the ribosomal DNA repeats, new chromosomal bands frequently appear in both the large and the small size ranges. Many of these are due to translocations (Thrash-Bingham & Gorman, 1992), but whether there are also deletions leading to aneuploid regions of the genome is not kno ...
Variation in biological properties of cauliflower mosaic virus clones
Variation in biological properties of cauliflower mosaic virus clones

... we then determined whether a range of variants could be detected by restriction enzyme analysis of virus DNA preparations. We chose passaged material of isolate 11/3 from which the three clonal groups described above (Fig. 2a) originated. The ancestry of the 11/3 clones is shown in Fig. 2(b). Viral ...
Two Oxidosqualene Cyclases Responsible for
Two Oxidosqualene Cyclases Responsible for

... The first committed step in triterpenoid biosynthesis is the cyclization of epoxysqualene into various triterpene alcohol isomers, a reaction catalyzed by oxidosqualene cyclases (OSCs). The different OSCs have characteristic product specificities, which are mainly due to differences in the numbers o ...
Aprotinin Petit havana
Aprotinin Petit havana

... observed during pollen development (Taylor et al. 1997), similarly in Lilium, the serine proteinase LIM9 is secreted into anther locules (Radlowski et al. 1996). Recently a gene, OsSIZ1, a small ubiquitin-related modifier (SUMO) E3 ligase that helps in anchoring SUMO to protein substrates for modif ...
A large scale analysis of resistance gene
A large scale analysis of resistance gene

... A. thaliana (eg. Simonich and Innes 1995; Gassmann et al. 1999; Deslandes et al. 2002) and in several other plant species (eg. Lawrence et al. 1995; Thomas et al. 1997; Milligan et al. 1998; Wang et al. 1999). R-genes can be divided into families based upon homologous domains in their protein produc ...
jsm2003 - University of Wisconsin–Madison
jsm2003 - University of Wisconsin–Madison

... • improve inference of complex genetic architecture – patterns and individual elements of epistasis – appropriate estimates of means, variances, covariances • asymptotically unbiased, efficient ...
PureLink® RNA Mini Kit - Thermo Fisher Scientific
PureLink® RNA Mini Kit - Thermo Fisher Scientific

... Cartridge containing a clear silica-based membrane to which the RNA binds. Any impurities are effectively removed by subsequent washing (Vogelstein & Gillespie, 1979). The purified total RNA is then eluted in RNase-Free Water (or Tris Buffer, pH 7.5) and may be used for use in a variety of downstrea ...
Construction of a pBAD24 Vector Containing σ32 : Restoration of
Construction of a pBAD24 Vector Containing σ32 : Restoration of

... pBAD24 between the EcoRI and XbaI restriction sites. Fig. 2A shows an excerpt of the sequencing results of rpoH. The sequencing primer, pBAD reverse primer, was provided by GENEWIZ and its location is indicated on Fig. 1A. The regions corresponding to the ligation point between rpoH and pBAD24 are d ...
Multiple Mechanisms Contribute to Lateral Transfer of an
Multiple Mechanisms Contribute to Lateral Transfer of an

... pir-116 cells by setting the electroporator (Gene Pulser, Bio-Rad Laboratories, USA) at 2.5 kV, 200V for 4.5 msec. After electroporation, the cells were plated on LB plates supplemented with kanamycin to select transformants. Colonies having plasmid pPDL2 were identified by performing colony PCR usin ...
Translocation Breakpoints Are Clustered on Both Chromosome 8
Translocation Breakpoints Are Clustered on Both Chromosome 8

... By using a probe synthesized on the basis of the published cDNA sequence, clones were isolated from a human genomic library spanning most of the intron centromeric to the fifth reported AMLl exon.7 This is the region where chromosome 21 breakpoints were suggested to cluster in the t(8;21). As the in ...
White Paper: DMET™ Plus allele translation
White Paper: DMET™ Plus allele translation

... indeed exist as unknown alleles (reported as “UNK”). Diplotypes with UNK calls are reported as alternative results in a separate field of the translation reports. Classification codes, useful for sorting and interpreting the comprehensive or summary translation output, are also reported. Report gene ...
1 BIOL2323: GENERAL GENETICS STUDY GUIDE
1 BIOL2323: GENERAL GENETICS STUDY GUIDE

... describe the steps of a typical cloning experiment know the meaning of the terms “restriction” and “modification” in bacteria describe how restriction enzyme recognition sites look like and which kinds of DNA ends are produced by restriction enzymes calculate the frequency of restriction sites expla ...
Mapping the histone code at hMLH1. - JScholarship
Mapping the histone code at hMLH1. - JScholarship

... 2002). More recently, it was shown that induction of a heat shock gene displaces both H3.3 and H3, followed by selective deposition of H3.3 (Wirbelauer et al., 2005). When comparing histone tail modification profiles of H3 and H3.3, although none of the modifications examined were exclusive to eithe ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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