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Supplementary Information
Supplementary Information

... ratio threshold = 0.8, deletion log2 ratio threshold = -0.5, baseline distribution (smoothing window size) = 10kbp, and p-value threshold = 0.05. The output of FACADE is list of copy number alterations in each sample profiled. Gene expression profiling RNA extracted from each PDAC line and HPDE was ...
Genetics Vocabulary 2014-2015
Genetics Vocabulary 2014-2015

... recessive allele – an allele that is masked when a dominant allele is present hybrid – an organism that has two different alleles for a trait phenotype – an organism’s physical appearance or visible traits gentotype – an organism’s genetic makeup or allele combinations homozygous – having two identi ...
microRNA: microRNA
microRNA: microRNA

... CLL pts, and in 16-1 causes decreased miRNA miR-15a and miR-16-1 directly target bcl2, and their absence may contribute to the pathogenesis of CLL miRNA or miRNA-like sequences may be used therapeutically to target bcl2 or other oncogenes in the future ...
Protein Synthesis (Transcription and Translation)
Protein Synthesis (Transcription and Translation)

... • A point mutation is a change in a single base pair in DNA. • A change in a single nitrogenous base can change the entire structure of a protein because a change in a single amino acid can affect the shape of the protein. ...
GENOME SEQUENCING AND OBJECTIVES
GENOME SEQUENCING AND OBJECTIVES

... millions of individual molecules. It expects to apply this technology to sequencing an individual human genome much more quickly and cheaply than can be done with current methods: The arrays could also be applied to studying interactions between other large sets. ...
lecture 03b
lecture 03b

... DNA replication Complementary base-pairing allows for a mechanism for 1 DNA molecule to be copied to produce two identical molecules. Because each new double-stranded molecule is made of one old strand and one new strand, this is called “semi-conservative” replication. ...
Document
Document

... • Upon completion of this lesson, the student will be able to  identify the basic cellular processes, which influence our health.  compare and contrast cellular adaptation processes.  describe the processes associated with aging and list the current theories.  determine syndromes associated with ...
Genetics
Genetics

... – Silent mutation: the strict definition = a change in the codon does not change the encoded amino acid; a more broad definition = a change that does not change the function of the encoded protein • by this definition a silent mutation could be any of these types of base substitions, as long as the ...
BIOLOGY CONTENT STANDARDS REVIEW
BIOLOGY CONTENT STANDARDS REVIEW

... 16. Draw and label a DNA molecule with nine base pairs, the transcribed mRNA molecule, and the resulting protein molecule. 17. Describe the three main types of RNA. Where is each found in the cell? Draw a picture of each. Base-pairing rules explain precise copying of DNA during semi-conservative rep ...
answers
answers

... Which kind of RNA has an ANTICODON? __t-RNA____ What kind of molecules make up ribosomes? ___PROTEINS______ & ___r-RNA__________ Which cell part makes r-RNA? ___NUCLEOLUS__ Which cell part makes proteins? _RIBOSOMES______________ The ribosome makes sure the amino acid is put in the right spot by mat ...
Identification of disease genes Mutational analyses Monogenic
Identification of disease genes Mutational analyses Monogenic

... No families, no linkage studies available (mutations cause low reproductive fitness). ...
1. DNA (genetic info is passed down through DNA and RNA) A
1. DNA (genetic info is passed down through DNA and RNA) A

... 7. incomplete dominance- red X white  pink; both protein product are expressed and blended 8. codominance- red x white  red and white; both protein products are equally expressed ex.AB blood types 9. multiple alleles- blood types- ABO 10. epistasis- one gene affects expression of another 11. linke ...
Cells - Troup County High School
Cells - Troup County High School

... from generation to generation. ...
FACS RNA
FACS RNA

... The   optimal   volume   of   RNAlater   and   limit   of   dilution   is   10%.     Therefore,   15   mL   conical   tubes   containing   9   mL   of   RNAlater   should   be   provided   to   the   Flow   Core   and   1  mL  of  so ...
`RNA world`.
`RNA world`.

... With RNA we throw away many of the rules! If DNA is straight-laced and uniform, RNA is freewheeling and audacious. RNA is the non-conformist. It has ceded primacy as the repository of genetic information to DNA but it has gained versatility. It is a master architect, forming complex, threedimension ...
Introduction and Review
Introduction and Review

... Transcription ends (termination) when RNA polymerase reaches a terminator sequence, usually located several bases upstream from where transcription actually stops Some terminators require a termination factor protein called the rho factor (); these are rho-dependent. Others are rhoindependent. Mess ...
Alternative Splicing
Alternative Splicing

... • mutations in the Alu can create a 5’ or 3’ site in an intron causing it to be an exon • This mutation doesn’t impact existing exons • It only has effect when it is alternatively spliced in ...
Protein
Protein

... Determine protein sequence ...
From Gene to Protein
From Gene to Protein

... • Facilitate mRNA export from the nucleus • Separated from the stop codon by trailer sequence ...
A grand challenge for nutrigenomics
A grand challenge for nutrigenomics

... Doll, 1992), diabetes is a nutritional/metabolic disorder, we are all familiar with the cholesterol–cardiovascular disease relationship, and obesity is a pressing nutritional problem, with a majority of Americans overweight and expanding rapidly (Ogden et al., 2007). If genetic scientists are lookin ...
3rd of 7 Review Packets
3rd of 7 Review Packets

... 7. incomplete dominance- red X white  pink; both protein product are expressed and blended 8. codominance- red x white  red and white; both protein products are equally expressed ex.AB blood types 9. multiple alleles- blood types- ABO 10. epistasis- one gene affects expression of another 11. linke ...
Legends for Supplementary Materials Figure S1. Schematic
Legends for Supplementary Materials Figure S1. Schematic

... Figure S3. Expression levels of LOG genes in wild type and log septuple mutant. (a) PCR for genotyping with genomic DNA prepared from wild type (WT), log1log2log3log4log5log7log8 septuple mutant (1234578), log1log2log3log4log5log7 (123457), and log1log2log3log4log5log8 (123458) with a pair of gene-s ...
SEGMENTAL VARIATION
SEGMENTAL VARIATION

... deal of human phenotypic variability including disease • Depth-of-coverage methods can detect many CNVs but not inversions and translocations. Variation from sample to sample limits sensitivity and specificity. ...
Gene Section PRAME (preferentially expressed antigen in melanoma) Atlas of Genetics and Cytogenetics
Gene Section PRAME (preferentially expressed antigen in melanoma) Atlas of Genetics and Cytogenetics

... LRR family proteins, some of which are known to have functions in cell immunity and signal transduction. It has been suggested that, like TLRs, PRAME may be upregulated in response to encounters with microbial pathogens, and may be involved in targeting intracellular PAMPs to the Golgi for ubiquityl ...
In meiosis, what is the difference between metaphase 1 and
In meiosis, what is the difference between metaphase 1 and

... 6. Explain what is meant by crossing-over (recombination). Be sure and mention at what time it is taking place within the cell. An exchange of corresponding segments between two homologous chromosomes at the chiasma. Occurs during prophase I of meiosis. ...
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RNA-Seq



RNA-seq (RNA sequencing), also called whole transcriptome shotgun sequencing (WTSS), is a technology that uses the capabilities of next-generation sequencing to reveal a snapshot of RNA presence and quantity from a genome at a given moment in time.
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