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Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein
Evolution of the Aldose Reductase-Related Gecko Eye Lens Protein

Isotope-Exchange Evidence that Glucose 6
Isotope-Exchange Evidence that Glucose 6

... into ATP molecules divided by the simultaneous rate of conversion of glucose 6-phosphate molecules into glucose molecules, increases with the MgATP concentration but is independent of the glucose concentration. This behaviour requires that glucose must bind before MgATP when the reaction is proceedi ...
Full-Text PDF
Full-Text PDF

... dose, and solid–liquid ratio on DPPH free radical scavenging, hydroxyl radical scavenging, degree of hydrolysis, and peptide yield were presented in Table 1. The hydroxyl radical scavenging rates of hydrolysate increased slightly with increasing pH values of the reaction system. However, the DPPH ra ...
Section 2: Energy Flow in Ecosystems
Section 2: Energy Flow in Ecosystems

... Efficiency of Cellular Respiration • Cells release energy most efficiently when oxygen is present because they make most of their ATP during aerobic respiration. ...
03-1 Metabolism of carbohydrate
03-1 Metabolism of carbohydrate

a review on biochemical mechanism of fatty acids synthesis and oil
a review on biochemical mechanism of fatty acids synthesis and oil

... In plants, fatty acid biosynthesis process initiated in plastid and ended in the endoplasmic reticulum. A series of biochemical steps are involved in TAG formation and many of the genes involved in this process have been identified. The quality of seed oil depends on the type of fatty acids esterifi ...
Comparative genomic analysis of carbon and nitrogen assimilation
Comparative genomic analysis of carbon and nitrogen assimilation

... The capability of microorganisms to fix atmospheric nitrogen plays an important role in recycling scarce nitrogen existing in nutrient-poor acidic conditions; however, the availability of nitrogen and the energy required for its fixation may limit bacterial growth and adversely affect the efficiency ...
Isolation of casein from milk
Isolation of casein from milk

... • Is a phosphoprotein, which has phosphate groups attached to some of the amino acid side chains. Mostly these amino acid are serine and threonine. • casein is a mixture of at least three similar proteins, which differ primarily in molecular weight and amount of phosphorus they contain (number of ph ...
Metabolism of Lipids
Metabolism of Lipids

Sulfur Metabolism in Escherichia coli and Related Bacteria: Facts
Sulfur Metabolism in Escherichia coli and Related Bacteria: Facts

... et al., 1998). This is why the synthesis of this latter molecule is also linked with hydrolysis of the ß,γ bond of GTP, which favors the reaction of sulfate incorporation (105-fold with respect to the reaction in absence of GTP). The coupling of the synthesis of APS with GTP hydrolysis displaces the ...
Trypsinogen from bovine pancreas Product Number T1143 Storage
Trypsinogen from bovine pancreas Product Number T1143 Storage

THE ROLE OF HYALURONIC ACID (HA) IN THE TREATMENT OF
THE ROLE OF HYALURONIC ACID (HA) IN THE TREATMENT OF

... • In vitro studies have shown that HA binds to CD44 receptors on the surfaces of cells that are involved in the inflammatory process.3,16,17 • The analgesic effects of intra-articular HA extend beyond its residence time in the osteoarthritic joint. • In vitro and in vivo studies have demonstrated th ...
Міністерство охорони здоров`я України Харківський
Міністерство охорони здоров`я України Харківський

... The material of manual is presented in 20 chapters. It includes structure and metabolism of basic classes of biomolecules (proteins, amino acids, nucleic acids, nucleotides, carbohydrates, lipids), regulation of metabolism and physiologic functions, biochemistry of enzymes, vitamins, blood, kidney, ...
Two Oxidosqualene Cyclases Responsible for
Two Oxidosqualene Cyclases Responsible for

... other pentacyclic triterpenoid products been described. That such OSCs with diverse product profiles exist can be seen from a recent report in which three Kalanchoe daigremontiana OSCs were shown to synthesize taraxerol, glutinol, and friedelin (i.e. products with carbon skeletons requiring relative ...
Crystallization and X Ray Diffraction Data Analyses of the Enzyme
Crystallization and X Ray Diffraction Data Analyses of the Enzyme

... formimine glutamase (EC 3.5.3.8). This complete pathway drives the conversion of histidine into glutamate, which in turn can be dea i ated i to α-ketoglutarate, allowing its complete oxidation. Urocanate hydratase, the second enzyme of the histidine-glutamate pathway, acts on the conversion of uroca ...
finalcarbohydrat met..
finalcarbohydrat met..

... glucose, galactose and fructose. They are absorbed from the jejunum to portal veins to the liver, where fructose and galactose are transformed into glucose. B.Two mechanisms are responsible for absorption of monosaccharides: active transport (against concentration gradient i.e. from low to high conc ...
C3/D8 Due: 2/21/14 Chem 151 Caffeine: Your Friend for Sleepless
C3/D8 Due: 2/21/14 Chem 151 Caffeine: Your Friend for Sleepless

... this enzyme affect how well or how fast caffeine is metabolized by the consumer. Known as single nucleotide polymorphisms (SNPs), these DNA Figure 2. Metabolic pathways for caffeine [5] sequences differ in one nucleotide which increases the amount of CYP1A2 enzymes in the liver, resulting in faster ...
Chem 356 Structure and Function in Biochemistry
Chem 356 Structure and Function in Biochemistry

... Soybeans and wheat contain starch, a polymer of glucose, which is broken down to glucose by microorganisms. The glucose is then broken down to pyruvate via glycolysis. Because the process is carried out in the absence of oxygen (i.e., it is fermentation), pyuvate is reduced to lactic acid and ethano ...
Carbohydrate Metabolism
Carbohydrate Metabolism

... A.The end products of carbohydrate digestion are monosaccharides: glucose, galactose and fructose. They are absorbed from the jejunum to portal veins to the liver, where fructose and galactose are transformed into glucose. B.Two mechanisms are responsible for absorption of monosaccharides: active tr ...
Anaerobic respiration with elemental sulfur and with disulfides
Anaerobic respiration with elemental sulfur and with disulfides

... Methanothermus, and Methanococcus, produce substantial amounts of H2 S, while methanogenesis is signi¢cantly reduced [34]. In some heterotrophs, such as Pyrococcus furiosus and Thermotoga maritima, sulfur is thought to serve as an additional electron sink, but in many organisms, e.g., Aquifex pyroph ...
POLYPEPTIDE SEQUENCING
POLYPEPTIDE SEQUENCING

... The two techniques most commonly used are I.E.F. followed by SDSPAG electrophoresis. Analysis of proteins is performed using MALDI - a new(-ish) mass spectrometry ionisation technique which uses lasers (Matrix Assisted ...


... 30 °C. The specific activities of the enzymes, concentrations of trace elements and the lipid content were determined at 24 h intervals. Cessation of lipid accumulation coincided with diminishing activities of the enzymes at 48 h. A significant decrease in metal ions concentration was observed follo ...
Sequence and evolutionary analysis of the human trypsin subfamily
Sequence and evolutionary analysis of the human trypsin subfamily

... change that completes the formation of the oxyanion hole and the substrate binding pocket, both of which are necessary for proper catalytic activity. Certain sequences in our alignment did not display conservation of this trypsin cleavage site, with substitutions at either the 15th or 16th positions ...
4.6 Fermentation
4.6 Fermentation

... 1. Cells contain only enough ATP for a few seconds of intense activity 2. Then cells rely on lactic acid fermentation (can supply for about 90 seconds) 3. Lactic acid build-up causes burning in muscles. Only way to get rid of lactic acid is chemical pathway that requires oxygen (why you breathe heav ...
Biochemical and Cellular Investigation of Vitreoscilla Hemoglobin
Biochemical and Cellular Investigation of Vitreoscilla Hemoglobin

... activity has been disclosed in VHb [25, 38]. The steadystate kinetic study has revealed that VHb catalyzes the oxidation of various aromatic substrates via a ping-pong mechanism, as normally found in horseradish peroxidase (HRP) [25]. However, this catalytic activity seems to be limited possibly as ...
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Enzyme



Enzymes /ˈɛnzaɪmz/ are macromolecular biological catalysts. Enzymes accelerate, or catalyze, chemical reactions. The molecules at the beginning of the process are called substrates and the enzyme converts these into different molecules, called products. Almost all metabolic processes in the cell need enzymes in order to occur at rates fast enough to sustain life. The set of enzymes made in a cell determines which metabolic pathways occur in that cell. The study of enzymes is called enzymology.Enzymes are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Enzymes' specificity comes from their unique three-dimensional structures.Like all catalysts, enzymes increase the rate of a reaction by lowering its activation energy. Some enzymes can make their conversion of substrate to product occur many millions of times faster. An extreme example is orotidine 5'-phosphate decarboxylase, which allows a reaction that would otherwise take millions of years to occur in milliseconds. Chemically, enzymes are like any catalyst and are not consumed in chemical reactions, nor do they alter the equilibrium of a reaction. Enzymes differ from most other catalysts by being much more specific. Enzyme activity can be affected by other molecules: inhibitors are molecules that decrease enzyme activity, and activators are molecules that increase activity. Many drugs and poisons are enzyme inhibitors. An enzyme's activity decreases markedly outside its optimal temperature and pH.Some enzymes are used commercially, for example, in the synthesis of antibiotics. Some household products use enzymes to speed up chemical reactions: enzymes in biological washing powders break down protein, starch or fat stains on clothes, and enzymes in meat tenderizer break down proteins into smaller molecules, making the meat easier to chew.
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