LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034
... 12. What is meant by lactose intolerance? How it can be overcome? 13. How the viscosity of starch solution is related to its structure? 14. Give a brief account of cyclodextrins and their use. 15. Explain the biological role of NAD+. 16. What are (i) oxidoreductases (ii) transferases. Give two examp ...
... 12. What is meant by lactose intolerance? How it can be overcome? 13. How the viscosity of starch solution is related to its structure? 14. Give a brief account of cyclodextrins and their use. 15. Explain the biological role of NAD+. 16. What are (i) oxidoreductases (ii) transferases. Give two examp ...
Molecular signatures-based prediction of enzyme
... problem of daunting complexity (Nobeli et al., 2009). Even though some progress has been done in this field (Carbonell et al., 2009; Gomez et al., 2003; Macchiarulo et al., 2004), there is still a need for better and more accurate methods of prediction. Our approach uses a graph-based representation ...
... problem of daunting complexity (Nobeli et al., 2009). Even though some progress has been done in this field (Carbonell et al., 2009; Gomez et al., 2003; Macchiarulo et al., 2004), there is still a need for better and more accurate methods of prediction. Our approach uses a graph-based representation ...
- TestbankU
... to peptide bonds, a form of covalent bond, secondary is due to hydrogen bonds between amino acids that are not directly connected to each other, tertiary is typically due to hydrophobic interactions – hydrophobic residues on the inside and hydrophilic residues on the outside with some disulphide cov ...
... to peptide bonds, a form of covalent bond, secondary is due to hydrogen bonds between amino acids that are not directly connected to each other, tertiary is typically due to hydrophobic interactions – hydrophobic residues on the inside and hydrophilic residues on the outside with some disulphide cov ...
BIOMG 3310: Principles of Biochemistry
... This also causes the loss of one positive and one negative charge, which changes the amino acid behavior because the behavior of the free amino acid is dependent upon the amino acid having two charges. ...
... This also causes the loss of one positive and one negative charge, which changes the amino acid behavior because the behavior of the free amino acid is dependent upon the amino acid having two charges. ...
Structure/function analyses of human serum paraoxonase (HuPON1
... organophosphatase activities. Until very recently, a three-dimensional model was not available for HuPON1, so functional roles have not been assigned to those residues. Based on sequence-structure alignment studies, we have folded the amino acid sequence of HuPON1 onto the sixfold h-propeller struct ...
... organophosphatase activities. Until very recently, a three-dimensional model was not available for HuPON1, so functional roles have not been assigned to those residues. Based on sequence-structure alignment studies, we have folded the amino acid sequence of HuPON1 onto the sixfold h-propeller struct ...
103 Lecture Ch21b
... • When product concentration is low, it dissociates from E1 and production is resumed • Feedback control allows products to be formed only when needed ...
... • When product concentration is low, it dissociates from E1 and production is resumed • Feedback control allows products to be formed only when needed ...
Lecture 4. The Screening Hypothesis.
... • Why the chemical co-evolution model to explain NP diversity is wrong! • How another evolutionary model, the Screening Hypothesis, provides a much more probable evolutionary scenario to explain not only NP abundance but also the properties of NPs. That potent and specific biological activity is a ...
... • Why the chemical co-evolution model to explain NP diversity is wrong! • How another evolutionary model, the Screening Hypothesis, provides a much more probable evolutionary scenario to explain not only NP abundance but also the properties of NPs. That potent and specific biological activity is a ...
High Throughput Screening Proposal
... Indicate if looking for a drug candidate or for a biochemical tool._________________________________ Biological event measuring (circle): protein-protein binding, enzymatic activity modulation, cell toxicity or other. Provide a brief description of the biology, rationale, and goals for conducting th ...
... Indicate if looking for a drug candidate or for a biochemical tool._________________________________ Biological event measuring (circle): protein-protein binding, enzymatic activity modulation, cell toxicity or other. Provide a brief description of the biology, rationale, and goals for conducting th ...
1-1 Amino Acids
... and to accept hydrogen bonds simultaneously. Cysteine, like histidine, is commonly found in enzyme active sites, because the thiolate anion is the most powerful nucleophile available from the naturally occurring amino acids. Amphipathic residues have both polar and nonpolar character, making them id ...
... and to accept hydrogen bonds simultaneously. Cysteine, like histidine, is commonly found in enzyme active sites, because the thiolate anion is the most powerful nucleophile available from the naturally occurring amino acids. Amphipathic residues have both polar and nonpolar character, making them id ...
Biology 30 The Chemistry of Living Things
... Nucleotide = building block of a nucleic acid; composed of: a) Pentose (5-C sugar) b) Nitrogeneous bases: c) Phosphate Group: 3 Examples of Nucleotide based molecules: 1. DNA = deoxyribonucleic acid (Fig 2.24) DNA: the double helix = 3-D shape = Watson & Crick => 1953 ...
... Nucleotide = building block of a nucleic acid; composed of: a) Pentose (5-C sugar) b) Nitrogeneous bases: c) Phosphate Group: 3 Examples of Nucleotide based molecules: 1. DNA = deoxyribonucleic acid (Fig 2.24) DNA: the double helix = 3-D shape = Watson & Crick => 1953 ...
Identification of the Missing Links in Prokaryotic Pentose Oxidation
... (Sso3118), and dopDH (Sso3117 ) were amplified by PCR from genomic DNA using Pfu TURBO polymerase (Stratagene) and cloned in expression vector pET24d (Novagen) (supplemental materials Table 1). The resulting plasmids were harvested from Escherichia coli HB101 transformants by Miniprep (Qiagen), sequ ...
... (Sso3118), and dopDH (Sso3117 ) were amplified by PCR from genomic DNA using Pfu TURBO polymerase (Stratagene) and cloned in expression vector pET24d (Novagen) (supplemental materials Table 1). The resulting plasmids were harvested from Escherichia coli HB101 transformants by Miniprep (Qiagen), sequ ...
Chapters 13 and 16
... E=citrate synthase Condensation reaction, Add the acetyl group of acetyl-CoA to oxaloacetate to form a 6C molecule (this step commits the acetyl group to the TCA cycle), ΔG=-31.4 kJ/mole (highly regulated enzyme), oxaloacetate must bind first, then acetyl-CoA can bind to the enzyme (sequential type ...
... E=citrate synthase Condensation reaction, Add the acetyl group of acetyl-CoA to oxaloacetate to form a 6C molecule (this step commits the acetyl group to the TCA cycle), ΔG=-31.4 kJ/mole (highly regulated enzyme), oxaloacetate must bind first, then acetyl-CoA can bind to the enzyme (sequential type ...
Cheng, P. T. ond J. E. Willis. Glucose-6-
... Rev. 25: 643), except that sodium nitrate was used in place of the ammonium salt ond tortrote was replaced by citric or molonic acid. All media were adjusted to pH 6.8 with NoOH before sterilization. After the given period of incubation the culture was filtered with suction. The cell moss was washed ...
... Rev. 25: 643), except that sodium nitrate was used in place of the ammonium salt ond tortrote was replaced by citric or molonic acid. All media were adjusted to pH 6.8 with NoOH before sterilization. After the given period of incubation the culture was filtered with suction. The cell moss was washed ...
Gibbs Free Energy Changes for the Glycolytic Enzymes
... ↓ TCA Cycle Glycolysis is the metabolic process of converting 1 molecule of glucose to 2 molecules of pyruvate through a series of 10 enzyme catalyzed reactions. All the steps of glycolysis occur in the cytosol of a cell and do not require O2. Note: converting pyruvate to acetyl-CoA and then acetyl- ...
... ↓ TCA Cycle Glycolysis is the metabolic process of converting 1 molecule of glucose to 2 molecules of pyruvate through a series of 10 enzyme catalyzed reactions. All the steps of glycolysis occur in the cytosol of a cell and do not require O2. Note: converting pyruvate to acetyl-CoA and then acetyl- ...
trimethylaminuria
... • Clinical Utility Gene Card of Trimethylaminuria www.eurogentest.org Click on trimethylaminuria to download PDF ...
... • Clinical Utility Gene Card of Trimethylaminuria www.eurogentest.org Click on trimethylaminuria to download PDF ...
Sample Question Set 5a
... 17. Calculate ΔG°’ for the oxidation of free FADH2 by O2. What is the maximum number of ATPs that can be synthesized, assuming standard conditions and 100% conservation of energy? (ΔG°’ = 30.5 kJ mol-1 for ATP synthesis) 18. When the F1 prtion of the ATP synthase complex is removed from the mitochon ...
... 17. Calculate ΔG°’ for the oxidation of free FADH2 by O2. What is the maximum number of ATPs that can be synthesized, assuming standard conditions and 100% conservation of energy? (ΔG°’ = 30.5 kJ mol-1 for ATP synthesis) 18. When the F1 prtion of the ATP synthase complex is removed from the mitochon ...
lecture CH21 chem131pikul UPDATED
... Denaturation is the process of altering the shape of a protein without breaking the amide bonds that form the primary structure: heat, acid, base, or agitation ...
... Denaturation is the process of altering the shape of a protein without breaking the amide bonds that form the primary structure: heat, acid, base, or agitation ...
Lab 7 - Creighton Biology
... In the main experiments, you will compare the activities of the two metabolic enzymes by measuring the rate of conversion of NADH into NAD+, again using a spectrophotometer. Spectrophotometric examinations of reactions are often referred to as colorimetric assays. The measure of total protein conten ...
... In the main experiments, you will compare the activities of the two metabolic enzymes by measuring the rate of conversion of NADH into NAD+, again using a spectrophotometer. Spectrophotometric examinations of reactions are often referred to as colorimetric assays. The measure of total protein conten ...
17 The Citric Acid Cycle: The latabolism of Acetyl
... generated (Fig 17-1). Since only a small quantity of oxaloacetate is needed to facilitate the conversion of a large quantity of acetyl units to CC>2, oxaloacetate may be considered to play a catalytic role. The ckric acid cycle is the mechanism by which much of the free energy liberated during the o ...
... generated (Fig 17-1). Since only a small quantity of oxaloacetate is needed to facilitate the conversion of a large quantity of acetyl units to CC>2, oxaloacetate may be considered to play a catalytic role. The ckric acid cycle is the mechanism by which much of the free energy liberated during the o ...
Problem Set 2 - University of Ottawa
... involves the elimination of one of the amino acid side chains that binds zinc. (/5) ...
... involves the elimination of one of the amino acid side chains that binds zinc. (/5) ...
Final Examination
... 8) The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pKaCOOH = 2.0; pKaNH2 = 10.6), and the mixture is spotted on a piece of filter paper and placed in an electric field at pH 6.15. Which statement is true? A) Both amino acids will move from the spot and be separate ...
... 8) The isoelectric point of alanine is pH = 6.15. It is mixed with proline (pKaCOOH = 2.0; pKaNH2 = 10.6), and the mixture is spotted on a piece of filter paper and placed in an electric field at pH 6.15. Which statement is true? A) Both amino acids will move from the spot and be separate ...
Enzyme
Enzymes /ˈɛnzaɪmz/ are macromolecular biological catalysts. Enzymes accelerate, or catalyze, chemical reactions. The molecules at the beginning of the process are called substrates and the enzyme converts these into different molecules, called products. Almost all metabolic processes in the cell need enzymes in order to occur at rates fast enough to sustain life. The set of enzymes made in a cell determines which metabolic pathways occur in that cell. The study of enzymes is called enzymology.Enzymes are known to catalyze more than 5,000 biochemical reaction types. Most enzymes are proteins, although a few are catalytic RNA molecules. Enzymes' specificity comes from their unique three-dimensional structures.Like all catalysts, enzymes increase the rate of a reaction by lowering its activation energy. Some enzymes can make their conversion of substrate to product occur many millions of times faster. An extreme example is orotidine 5'-phosphate decarboxylase, which allows a reaction that would otherwise take millions of years to occur in milliseconds. Chemically, enzymes are like any catalyst and are not consumed in chemical reactions, nor do they alter the equilibrium of a reaction. Enzymes differ from most other catalysts by being much more specific. Enzyme activity can be affected by other molecules: inhibitors are molecules that decrease enzyme activity, and activators are molecules that increase activity. Many drugs and poisons are enzyme inhibitors. An enzyme's activity decreases markedly outside its optimal temperature and pH.Some enzymes are used commercially, for example, in the synthesis of antibiotics. Some household products use enzymes to speed up chemical reactions: enzymes in biological washing powders break down protein, starch or fat stains on clothes, and enzymes in meat tenderizer break down proteins into smaller molecules, making the meat easier to chew.