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SensiFAST™ Two-Step Real-Time RT-PCR
SensiFAST™ Two-Step Real-Time RT-PCR

... RT reaction is diluted into the real-time PCR in the second ...
(III) ion and a cobalt (II) - Iowa State University Digital Repository
(III) ion and a cobalt (II) - Iowa State University Digital Repository

... a cobalt (II) macrocyclic complex with various substrates " (1987). Retrospective Theses and Dissertations. Paper 8619. ...
Molecular Modeling of Substrate Binding in Wild
Molecular Modeling of Substrate Binding in Wild

... procedure for commercial production of vitamin C using genetically modified bacteria expressing the gene for 2,5 diketo-D-gluconic acid reductase.1,2 These genetic manipulations have resulted in bacteria that can directly produce 2-KLG from D-glucose. In principle, this method may have considerable ...
CHEM 1411 – STUDY-GUIDE-for-TEST-2
CHEM 1411 – STUDY-GUIDE-for-TEST-2

... 56. During volcanic eruptions, hydrogen sulfide gas is given off and oxidized by air according to the following chemical equation: 2H2S(g) + 3O2(g)  2SO2(g) + 2H2O(g) Calculate the standard enthalpy change for the above reaction given: 3S(s) + 2H2O(g)  2H2S(g) + SO2(g) H° = 146.9 kJ/mol S(s) + O2 ...
PDF - DigiNole! - Florida State University
PDF - DigiNole! - Florida State University

... diketo-D-gluconic acid reductase.1,2 These genetic manipulations have resulted in bacteria that can directly produce 2-KLG from D-glucose. In principle, this method may have considerable advantage over the currently used Reichstein and Grussner synthesis, which involves numerous chemical steps in ad ...
Oxidation-Reduction Reactions
Oxidation-Reduction Reactions

... It is important to remember that the two half-reactions occur simultaneously. The resulting ions that are formed are then attracted to one another, forming an ionic bond. Redox reactions are also commonly described in terms of oxidizing and reducing agents. In the reaction above, the zinc is being o ...
Determination of Equilibrium Constants for the
Determination of Equilibrium Constants for the

... at the B3LYP/6-311++G(3df,3pd) level. These complexes are similar to the well-known adducts between HO2 and other atmospheric species such as H2O. Hermans et al.7,17 also performed approximated G2Mc// B3LYP/cc-pVTZ calculations on the reactions of HO2 with these carbonyls, but they went one step fur ...
Recent Developments on the Mechanism and Kinetics
Recent Developments on the Mechanism and Kinetics

... Krause et al., 2009; Martínez et al., 2011). The vast majority of esters can be prepared using esterification reaction in the chemical engineering industry. Esterification has acquired further improvement from the engineering side; this mainly depends on the research of esterification kinetics. On ...
Presence of Anaplerotic Reactions and Transamination, and the
Presence of Anaplerotic Reactions and Transamination, and the

... and Stitt (1983~).(ii) Aconitase (EC 4.2.1.3) was assayed by a modification of the method of Goldberg & Ellis (1983). The reaction mixture (1.0 ml) contained 100 mM-HEPES/NaOH (pH 7-4), 1.7 mM-MnCl,, 0.15 mMNADP+, 1 U isocitrate dehydrogenase and cell extract. The reaction was started with 5 mwcitri ...
Elsevier Scientific Publishing Company, Amsterdam
Elsevier Scientific Publishing Company, Amsterdam

... 3. Bilayer-forming saturated phosphatidylcholines produced activation which was maximal at 9 carbon atoms in each chain but decreased sharply as the chainlength was increased and essentially disappeared at 14 carbon atoms. By contrast the oligomycin-sensitivity increased with the increase in chain l ...
Carbamoyl Phosphate Synthetase: Closure of the B
Carbamoyl Phosphate Synthetase: Closure of the B

... 933, respectively) are topologically similar but not identical to one another as can be seen in Figure 2a. This observation is in full accord with the different biochemical functions required of these two synthetase components. Each one of these two active sites must utilize Mg2+ATP to phosphorylate ...
18. enzymes iii
18. enzymes iii

... ∆G°′ for the reaction is negative and the equilibrium favours P. This equilibrium is not affected by any catalyst. A favourable equilibrium, however, does not mean that S → P conversion is fast. The rate of a reaction, in fact, depends on an entirely different parameter. There exists an energetic ba ...
12_chemistry_impq_CH13_amines_02
12_chemistry_impq_CH13_amines_02

... The hydrogen attached to nitrogen in sulphonamide is strongly acidic due to the presence of strong electron withdrawing sulphonyl group. Hence, it is soluble in alkali. (b) In the reaction with secondary amine, N,N-diethylbenzenesulphonamide is formed. ...
Appendix
Appendix

... at the end point, the purity of the KHP, the molar mass for KHP, and the titration’s repeatability. Having established these, we can combine them to arrive at the final uncertainty. Uncertainty in the Mass of KHP. After drying the KHP, we store it in a sealed container to prevent it from readsorbing ...
Structure and Function of Mammalian Ribosomes
Structure and Function of Mammalian Ribosomes

... acid, were always inactive in polypeptide synthesis (Lamfrom & Glowacki, 1962 : Lazda & Noll, personal communication). In 1968 Schweet and coworkers reported that reticulocyte ribosomes pelleted after treatment with 0.5 ~-Kc1 were still competent in poly U-directed polyphenylalanine synthesis, and, ...
GEOCHEMICAL AND BIOGEOCHEMICAL
GEOCHEMICAL AND BIOGEOCHEMICAL

... but biogeochemical reaction processes. At the same time, reaction modeling is now commonly coupled to the problem of mass transport in groundwater flows, producing a subfield known as reactive transport modeling. Whereas a decade ago such modeling was the domain of specialists, improvements in mathema ...
Chap15 - Bakersfield College
Chap15 - Bakersfield College

... –Much like water in a U-shaped tube, there is constant mixing back and forth through the lower portion of the tube. “reactants” ...
Click Chemistry in Peptide-Based Drug Design
Click Chemistry in Peptide-Based Drug Design

... cyclization methods were applied. In the first method, after removing side-chain protection with trifluoroacetic acid, 2 equiv of CuI and 50 equiv of N,N-diisopropylethylamine were added and kept overnight. Following cleavage from the resin with 1% NaOH, the cyclic peptide was obtained in 76% yield ...
1. What energy changes occur when chemical bonds are formed
1. What energy changes occur when chemical bonds are formed

... (iv) If the ammonia was produced as a liquid and not as a gas, state and explain the effect this would have on the value of Hο for the reaction. ...
Reaction Rates
Reaction Rates

... amount of product increases. If you know the change in a product or a reactant during a segment of time, you can calculate the average rate of the reaction. Most often, chemists are concerned with changes in the molar concentration (mol/L, M) of a reactant or product during a reaction. Therefore, th ...
Chapter 16: Reaction Rates
Chapter 16: Reaction Rates

... For many reactions, the process from reactants to products is reversible. Figure 16.6 illustrates the reverse endothermic reaction between CO 2 and NO to re-form CO and NO 2. In this reaction, the reactants lie at a low energy level. They must overcome a significant activation energy to re-form CO a ...
Kinetics Workbook - School District 67
Kinetics Workbook - School District 67

... An 8.00 g piece of magnesium was placed into 6.0 M HCl . After 25 s. 3.50 g of unreacted magnesium remained. The average rate at which magnesium was consumed is: A. ...
Test bank questions
Test bank questions

... 50.0 g of N2O4 is introduced into an evacuated 2.00 L vessel and allowed to come to equilibrium with its decomposition product, N2O4(g) 2NO2(g). For this reaction Kc = 0.133. Once the system has reached equilibrium, 5.00 g of NO 2 is injected into the vessel, and the system is allowed to equilibrate ...
Sample lab exercise using Mimulus Pollination
Sample lab exercise using Mimulus Pollination

... You will also know which pollinator is actively pollinating (Visit table). Each bee visit is as effective as a bird visit for pollination efficiency. In most of our simulation we will have an equal number of bee and bird visits, so the setting should be 1 for each, unless specified. ...
MOL WS 2016 Handout T3 Metabolism RNA world
MOL WS 2016 Handout T3 Metabolism RNA world

... Ribonuclease P (RNase P) is a type of ribonuclease which cleaves RNA. RNase P is unique from other RNases in that it is a ribozyme – a ribonucleic acid that acts as a catalyst in the same way that a protein based enzyme would. Its function is to cleave off an extra, or precursor, sequence of RNA on ...
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Multi-state modeling of biomolecules

Multi-state modeling of biomolecules refers to a series of techniques used to represent and compute the behaviour of biological molecules or complexes that can adopt a large number of possible functional states.Biological signaling systems often rely on complexes of biological macromolecules that can undergo several functionally significant modifications that are mutually compatible. Thus, they can exist in a very large number of functionally different states. Modeling such multi-state systems poses two problems: The problem of how to describe and specify a multi-state system (the ""specification problem"") and the problem of how to use a computer to simulate the progress of the system over time (the ""computation problem""). To address the specification problem, modelers have in recent years moved away from explicit specification of all possible states, and towards rule-based formalisms that allow for implicit model specification, including the κ-calculus, BioNetGen, the Allosteric Network Compiler and others. To tackle the computation problem, they have turned to particle-based methods that have in many cases proved more computationally efficient than population-based methods based on ordinary differential equations, partial differential equations, or the Gillespie stochastic simulation algorithm. Given current computing technology, particle-based methods are sometimes the only possible option. Particle-based simulators further fall into two categories: Non-spatial simulators such as StochSim, DYNSTOC, RuleMonkey, and NFSim and spatial simulators, including Meredys, SRSim and MCell. Modelers can thus choose from a variety of tools; the best choice depending on the particular problem. Development of faster and more powerful methods is ongoing, promising the ability to simulate ever more complex signaling processes in the future.
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