Biocatalytic degradation of pollutants
... Ralstonia eutropha JMP134(pJP4), which grows on the herbicide 2,4-dichlorophenoxyacetate (2,4D), showed an inducible chemotactic response to 2,4D [22]. The response required the presence of the TfdK permease, a member of the major facilitator superfamily of transport proteins. These reports are of i ...
... Ralstonia eutropha JMP134(pJP4), which grows on the herbicide 2,4-dichlorophenoxyacetate (2,4D), showed an inducible chemotactic response to 2,4D [22]. The response required the presence of the TfdK permease, a member of the major facilitator superfamily of transport proteins. These reports are of i ...
Ethylene Glycol Intoxication
... - severe metabolic acidosis (high anion gap) from glycolic acid accumulation - very high lactate (artefactual as there is high cross reactivity between lactate and glycolate in laboratory analysis) -> high lactate with oxidase method, less high with lactate dehydrogenase method - high osmolar gap - ...
... - severe metabolic acidosis (high anion gap) from glycolic acid accumulation - very high lactate (artefactual as there is high cross reactivity between lactate and glycolate in laboratory analysis) -> high lactate with oxidase method, less high with lactate dehydrogenase method - high osmolar gap - ...
ภาพนิ่ง 1
... enzyme will utilize (an example will be discussed). • It is also useful for comparing similar enzymes from different tissues or different organisms. • Also, it is the Km of the rate-limiting enzyme in many of the biochemical metabolic pathways that determines the amount of product and overall regula ...
... enzyme will utilize (an example will be discussed). • It is also useful for comparing similar enzymes from different tissues or different organisms. • Also, it is the Km of the rate-limiting enzyme in many of the biochemical metabolic pathways that determines the amount of product and overall regula ...
Introduction to Enzymes - Rose
... protons. Many organic chemical reactions are enhanced by altered pH; although the pH in the overall environment is fixed by other factors, the enzyme can use specific functional groups to alter the effective pH in the active site. In other words, the cell can have an internal pH of 7.4, but due to t ...
... protons. Many organic chemical reactions are enhanced by altered pH; although the pH in the overall environment is fixed by other factors, the enzyme can use specific functional groups to alter the effective pH in the active site. In other words, the cell can have an internal pH of 7.4, but due to t ...
Requirements for respiration and energy generation, ion
... 10. Allison SL, Phillips AT (1990) Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Pseudomonas putida. J Bacteriol 172: 5470-5476. ...
... 10. Allison SL, Phillips AT (1990) Nucleotide sequence of the gene encoding the repressor for the histidine utilization genes of Pseudomonas putida. J Bacteriol 172: 5470-5476. ...
PAGES 1-41 INCL. 1. Overview (a) discovery of enzymes (b
... organization (x-ray crystal structure of lysozyme in 1965) and some knowledge of their mechanism of action (Fischer's lock & key model - 1894) by detailed kinetic investigation. - present day can easily synthesize proteins by recombinant DNA methodology (1st chemical synthesis in 1969) and manipulat ...
... organization (x-ray crystal structure of lysozyme in 1965) and some knowledge of their mechanism of action (Fischer's lock & key model - 1894) by detailed kinetic investigation. - present day can easily synthesize proteins by recombinant DNA methodology (1st chemical synthesis in 1969) and manipulat ...
consistency-checker
... slot of a frame that is not suppose to have such a value. (this means errors are often listed multiple times, under different frames) The checker also flags cardinality violations. For example, cases where more than one value is present in a slot that is only allowed to have a single value. ...
... slot of a frame that is not suppose to have such a value. (this means errors are often listed multiple times, under different frames) The checker also flags cardinality violations. For example, cases where more than one value is present in a slot that is only allowed to have a single value. ...
Enzymes How Do Enzymes Work?
... 1. Draw the Lewis structure of the dipeptide Val-Ser. (Hint: The structures of these amino acids appear on the “Examples of Amino Acids” slide in this presentation.) 2. The amino acid valine has a hydrocarbon sidechain. In the native (folded) structure of a water-soluble protein, would you expect to ...
... 1. Draw the Lewis structure of the dipeptide Val-Ser. (Hint: The structures of these amino acids appear on the “Examples of Amino Acids” slide in this presentation.) 2. The amino acid valine has a hydrocarbon sidechain. In the native (folded) structure of a water-soluble protein, would you expect to ...
acid base self study with practice questions
... Nausea and vomiting As with most acid-base imbalances, the treatment of metabolic acidosis is dependent upon the cause. The presence of metabolic acidosis should spur a search for hypoxic tissue somewhere in the body. Hypoxemia can lead to anaerobic metabolism system-wide, but hypoxia of any tissue ...
... Nausea and vomiting As with most acid-base imbalances, the treatment of metabolic acidosis is dependent upon the cause. The presence of metabolic acidosis should spur a search for hypoxic tissue somewhere in the body. Hypoxemia can lead to anaerobic metabolism system-wide, but hypoxia of any tissue ...
GST SF in E. coli - Institute for Genomic Biology
... • Application of differential labeling and multiple chromatographies for each metabolomics experiment to increase accuracy • Continued and increasing collaboration with the BPs and Cores ...
... • Application of differential labeling and multiple chromatographies for each metabolomics experiment to increase accuracy • Continued and increasing collaboration with the BPs and Cores ...
Project Manual Bio3055 Metabolic Disease: Hypoxanthine
... Metabolic disease is a broad term that is generally used to describe diseases that result from enzyme deficiencies in either catabolic or biosynthesis pathways. When these enzyme deficiencies occur, there is not only a decrease in the products of the pathway, but the intermediates in the pathway als ...
... Metabolic disease is a broad term that is generally used to describe diseases that result from enzyme deficiencies in either catabolic or biosynthesis pathways. When these enzyme deficiencies occur, there is not only a decrease in the products of the pathway, but the intermediates in the pathway als ...
Document
... • There are thousands of different kinds of proteins in any particular cell (such as a lymphocyte). ...
... • There are thousands of different kinds of proteins in any particular cell (such as a lymphocyte). ...
Enzyme Structure
... The enzyme is then free to start again. The end result is the same (SP), but a different route is taken, so that the S P reaction as such never takes place. In by-passing this step, the reaction can be made to happen much more quickly. ...
... The enzyme is then free to start again. The end result is the same (SP), but a different route is taken, so that the S P reaction as such never takes place. In by-passing this step, the reaction can be made to happen much more quickly. ...
Exam#2-`95
... 5. Lactate diffuses through the sarcolemma of muscle to get into blood. 6. For every 2 molecules of acetyl CoA produced, carbohydrate catabolism yields more net ATP than fat catabolism. 7. Cycling at a cadence of 80 rev/min and a load of 2.75 kg, results in 298 Watts. 8. The myosin ATPase in muscle ...
... 5. Lactate diffuses through the sarcolemma of muscle to get into blood. 6. For every 2 molecules of acetyl CoA produced, carbohydrate catabolism yields more net ATP than fat catabolism. 7. Cycling at a cadence of 80 rev/min and a load of 2.75 kg, results in 298 Watts. 8. The myosin ATPase in muscle ...
Dr: Anwar J almzaiel Glycolysis
... H2O may divide into an anaerobic (without oxygen) and aerobic (with oxygen) phases. Theses phases do not involve separate pathways, but that an initial anaerobic phase continuing directly in an aerobic phase in the presence of oxygen. Glycolysis: is the term applied to the production of lactic acid ...
... H2O may divide into an anaerobic (without oxygen) and aerobic (with oxygen) phases. Theses phases do not involve separate pathways, but that an initial anaerobic phase continuing directly in an aerobic phase in the presence of oxygen. Glycolysis: is the term applied to the production of lactic acid ...
evaluation of cirrhosis liver disease via protein-protein
... Processes(BP) (21). In fact, DAVID Gene Functional Classification Tool ( http://david.abcc.ncifcrf.gov ) provides a list of associated biological terms into organized classes of related genes by using a novel algorithm (22). Functional annotation software DAVID online program can provide functional ...
... Processes(BP) (21). In fact, DAVID Gene Functional Classification Tool ( http://david.abcc.ncifcrf.gov ) provides a list of associated biological terms into organized classes of related genes by using a novel algorithm (22). Functional annotation software DAVID online program can provide functional ...
Cofactors
... Homologous enzymes catalyze related reactions; this is how trp and his biosynthesis enzymes seem to have evolved Variant: recruit some enzymes from another pathway without duplicating the whole thing (example: ubiquitination) ...
... Homologous enzymes catalyze related reactions; this is how trp and his biosynthesis enzymes seem to have evolved Variant: recruit some enzymes from another pathway without duplicating the whole thing (example: ubiquitination) ...
Simulating the physiology of athletes during endurance
... The energy conversion relevant to propel the bicycle takes place in muscle. During cycling, less than a quarter of the energy obtained by burning nutrients with oxygen is converted to external work. The remainder of the energy is converted to heat, which is transported from the muscle by thermal con ...
... The energy conversion relevant to propel the bicycle takes place in muscle. During cycling, less than a quarter of the energy obtained by burning nutrients with oxygen is converted to external work. The remainder of the energy is converted to heat, which is transported from the muscle by thermal con ...
Chapter 12 Lecture Notes: Metabolism – Enzyme and Gene
... 1. Bacteria are extremely efficient organisms. It is wasteful to have all 1000 – 2000 metabolic pathways on at the same time. 2. During exponential growth all cellular components are synthesized at constant rates relative to one another (balanced growth). 3. Thus, the cell integrates signals regardi ...
... 1. Bacteria are extremely efficient organisms. It is wasteful to have all 1000 – 2000 metabolic pathways on at the same time. 2. During exponential growth all cellular components are synthesized at constant rates relative to one another (balanced growth). 3. Thus, the cell integrates signals regardi ...
Six Major Classes of Enzymes and Examples of Their Subclasses
... Biologic catalysts 1. Shared properties with chemical catalysts a. Enzymes are neither consumed nor produced during the course of a reaction. b. Enzymes do not cause reactions to take place, but they greatly enhance the rate of reactions that would proceed much slower in their absence. They alter t ...
... Biologic catalysts 1. Shared properties with chemical catalysts a. Enzymes are neither consumed nor produced during the course of a reaction. b. Enzymes do not cause reactions to take place, but they greatly enhance the rate of reactions that would proceed much slower in their absence. They alter t ...
Introductory presentation(, 9.8 MB)
... Non-human species widely studied to understand human disease. ...
... Non-human species widely studied to understand human disease. ...
Hybrid System Modeling and Identification of Cell Biology Systems
... • approximation: the underlying dynamics, e.g. enzyme kinetics, are not known or specified a priori and collected data is not reliable enough to deduce a non-linear relationship and/or; • analysis: the original non-linear system is to difficult to analyze and an (approximative) alternative is sought ...
... • approximation: the underlying dynamics, e.g. enzyme kinetics, are not known or specified a priori and collected data is not reliable enough to deduce a non-linear relationship and/or; • analysis: the original non-linear system is to difficult to analyze and an (approximative) alternative is sought ...
Catalysis by Enzymes
... Catalysis by Enzymes • Enzyme A protein that acts as a catalyst for a biochemical reaction. ...
... Catalysis by Enzymes • Enzyme A protein that acts as a catalyst for a biochemical reaction. ...
Document
... and the gene list from the differentially expressed genes –Reformats the KDA output for Cytoscape –Launches Cytoscape to visualize the results ...
... and the gene list from the differentially expressed genes –Reformats the KDA output for Cytoscape –Launches Cytoscape to visualize the results ...
Metabolic network modelling
Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑