Application of stable isotopes and mass isotopomer distribution
... appearance of [U-13Cn] isotopomers can only derive from the administered tracer (e.g., dietary [U-13C6]dextrin, n = 6). Therefore, when [U-13Cn] compounds ([M+n]) are administered, appearance of the [M+n] isotopomer in, for example, plasma glucose when [U-13C6]glucose is administered (Figure 1), rep ...
... appearance of [U-13Cn] isotopomers can only derive from the administered tracer (e.g., dietary [U-13C6]dextrin, n = 6). Therefore, when [U-13Cn] compounds ([M+n]) are administered, appearance of the [M+n] isotopomer in, for example, plasma glucose when [U-13C6]glucose is administered (Figure 1), rep ...
Systems biology of inborn errors of metabolism
... leading to life threatening conditions when left un-treated. IEMs not only demand better diagnostic methods and efficient therapeutic regimen, but also, a high level understanding of the precise biochemical pathology involved. Constraint-based metabolic network reconstruction and modeling is the cor ...
... leading to life threatening conditions when left un-treated. IEMs not only demand better diagnostic methods and efficient therapeutic regimen, but also, a high level understanding of the precise biochemical pathology involved. Constraint-based metabolic network reconstruction and modeling is the cor ...
Integration of omics data with biochemical reaction
... The appearance of omics data sets has contributed to the rapid development of systems biology, which seeks the understanding of complex biological systems. Constraint-based modeling is one modeling formalism applied in systems biology, which relies on genome-scale network reconstructions. Metabolic ...
... The appearance of omics data sets has contributed to the rapid development of systems biology, which seeks the understanding of complex biological systems. Constraint-based modeling is one modeling formalism applied in systems biology, which relies on genome-scale network reconstructions. Metabolic ...
Local BLAST - For link to GMS6014, click here
... Protein family / Protein domain Interaction Pathway ...
... Protein family / Protein domain Interaction Pathway ...
LIMS for the Masses
... gene, protein and tissue changes happen in minutes, hours or days • Allows easy longitudinal monitoring of patient (or organ) function (pre&post op) ...
... gene, protein and tissue changes happen in minutes, hours or days • Allows easy longitudinal monitoring of patient (or organ) function (pre&post op) ...
Strong dominance of functional alleles over gene deletions in
... cells (initially 5 lL of the starving culture, then incrementally more as recurrent refilling of the starving cultures to approximately 200 lL with water caused gradual dilution). To test for the presence of viable cells, samples were transferred to fresh YPD and incubated for 3 days. The day of sta ...
... cells (initially 5 lL of the starving culture, then incrementally more as recurrent refilling of the starving cultures to approximately 200 lL with water caused gradual dilution). To test for the presence of viable cells, samples were transferred to fresh YPD and incubated for 3 days. The day of sta ...
Metabolic flux profiling of recombinant protein secreting Pichia
... notably, Rol secretion levels obtained in P. pastoris high cell density cultures are rather moderate (around 300~500 mg L−1) [24]. To characterise the potential intracellular carbon flux redistribution due to recombinant Rol secretion, we performed chemostat aerobic cultivations using a fixed mixt ...
... notably, Rol secretion levels obtained in P. pastoris high cell density cultures are rather moderate (around 300~500 mg L−1) [24]. To characterise the potential intracellular carbon flux redistribution due to recombinant Rol secretion, we performed chemostat aerobic cultivations using a fixed mixt ...
Lecture PPT
... A is the frequency of collisions with the proper orientation to produce a chemical reaction. Can be as fast as 1013sec-1, which is about the frequency of collision in liquids. Thus, Arrhenius theory says that the rate constant is determined by i) the ratio of EA to T and ii) by the frequency of coll ...
... A is the frequency of collisions with the proper orientation to produce a chemical reaction. Can be as fast as 1013sec-1, which is about the frequency of collision in liquids. Thus, Arrhenius theory says that the rate constant is determined by i) the ratio of EA to T and ii) by the frequency of coll ...
Lehninger Principles of Biochemistry 5/e
... Most aa are metabolized in the liver The ammonia generated in this process is recycled and used in a variety of biosynthetic pathway The excess is either excreted directly or converted to urea or uric acid for excretion Excess ammonia generated in other tissues travels to the liver for conversion to ...
... Most aa are metabolized in the liver The ammonia generated in this process is recycled and used in a variety of biosynthetic pathway The excess is either excreted directly or converted to urea or uric acid for excretion Excess ammonia generated in other tissues travels to the liver for conversion to ...
Document
... respiration, the breakdown of glucose in the presence of oxygen) • Anabolic pathways consume energy to build complex molecules from simpler ones (synthesis of protein from amino acids) ...
... respiration, the breakdown of glucose in the presence of oxygen) • Anabolic pathways consume energy to build complex molecules from simpler ones (synthesis of protein from amino acids) ...
BS3050 Physiology of Sport and Exercise
... The rationale of training is to overload the muscular system regularly during training so that the tissue adapts gradually to the extra load. It follows therefore that overtraining is counterproductive because it depletes glycogen too much or causes the onset of acidosis too quickly Endurance Traini ...
... The rationale of training is to overload the muscular system regularly during training so that the tissue adapts gradually to the extra load. It follows therefore that overtraining is counterproductive because it depletes glycogen too much or causes the onset of acidosis too quickly Endurance Traini ...
![[1] What are arterial blood gases? ANSWER](http://s1.studyres.com/store/data/000508765_1-754da755301cfc706fc377ea3ffdb7a9-300x300.png)
[1] What are arterial blood gases? ANSWER
... cause direct arterial damage, to put the artery into spasm (“vasospasm”) or to impair the circulation of tissues supplied by the artery being stabbed. Hematoma formation is particularly common, something especially important to think about in patients with a coagulopathy (impaired clotting system), ...
... cause direct arterial damage, to put the artery into spasm (“vasospasm”) or to impair the circulation of tissues supplied by the artery being stabbed. Hematoma formation is particularly common, something especially important to think about in patients with a coagulopathy (impaired clotting system), ...
A Fast Semi-Automated Quantitative PCR Method for - Bio-Rad
... The use of SsoAdvanced Supermixes and PrimePCR PreAmp Assays is covered by one or more of the following U.S. patents and corresponding patent claims outside the U.S.: 5,804,375; 5,994,056; and 6,171,785. The purchase of these products includes a limited, non-transferable immunity from suit under the ...
... The use of SsoAdvanced Supermixes and PrimePCR PreAmp Assays is covered by one or more of the following U.S. patents and corresponding patent claims outside the U.S.: 5,804,375; 5,994,056; and 6,171,785. The purchase of these products includes a limited, non-transferable immunity from suit under the ...
Appendix B HISS Codes for Metabolic Investigations
... any necessary acute pre-analytical handling and appropriate storage are performed within the local laboratory. The Yorkhill Biochemistry Laboratory aims to produce results in a timely manner. Due to the complex nature of many analytical methods, some results may take several days/weeks. Where an ind ...
... any necessary acute pre-analytical handling and appropriate storage are performed within the local laboratory. The Yorkhill Biochemistry Laboratory aims to produce results in a timely manner. Due to the complex nature of many analytical methods, some results may take several days/weeks. Where an ind ...
Ethylene Glycol Poisoning
... Readily absorbed from the GI tract within 30-60 minutes Maximal blood concentration reached in 1-4 hours 1/2 life is 3-8 hours Lethal dose is estimated as 1-1.5 mls/kg or 100mls ...
... Readily absorbed from the GI tract within 30-60 minutes Maximal blood concentration reached in 1-4 hours 1/2 life is 3-8 hours Lethal dose is estimated as 1-1.5 mls/kg or 100mls ...
Powerpoint template for scientific posters
... and estimate that the catalogue of human ECM proteins and their interactors may exceed 2500 proteins. If current estimates for the number of genes in the human genome are true (approximately 30,000) this implies that approaching 10% of the human genome is dedicated to dealing with extracellular orga ...
... and estimate that the catalogue of human ECM proteins and their interactors may exceed 2500 proteins. If current estimates for the number of genes in the human genome are true (approximately 30,000) this implies that approaching 10% of the human genome is dedicated to dealing with extracellular orga ...
Hypersensitivity Disorders Allergic Emergencies
... ƒ Act by competitively inhibiting H1 & H2 receptors ƒ Diphenhydramine is best single agent against pruritis, but combo Rx (with H2 blocker) is superior ƒ Give PO for mild & local reactions ƒ Give IM only if airway compromise & unable to start ...
... ƒ Act by competitively inhibiting H1 & H2 receptors ƒ Diphenhydramine is best single agent against pruritis, but combo Rx (with H2 blocker) is superior ƒ Give PO for mild & local reactions ƒ Give IM only if airway compromise & unable to start ...
Enzymes - WordPress.com
... • Inorganic – the help the substrate and enzyme bond but are not changed or used in any way • Example Cl- for amylase • Organic – They are called coenzymes and participate in the reaction and are changed by it (second substrate) they also recycle • Example Vitamins e.g. NAD is derived from vitamin B ...
... • Inorganic – the help the substrate and enzyme bond but are not changed or used in any way • Example Cl- for amylase • Organic – They are called coenzymes and participate in the reaction and are changed by it (second substrate) they also recycle • Example Vitamins e.g. NAD is derived from vitamin B ...
video slide
... Allosteric Regulation of Enzymes • Allosteric regulation – Is the term used to describe any case in which a protein’s function at one site is affected by binding of a regulatory molecule at another site – Function may be activated or inhibited. ...
... Allosteric Regulation of Enzymes • Allosteric regulation – Is the term used to describe any case in which a protein’s function at one site is affected by binding of a regulatory molecule at another site – Function may be activated or inhibited. ...
File
... Although the lock and key model is an obvious staging post, ensure that students can distinguish between it and the induced fit model. It is useful to relate the structure of an enzyme and the specificity of the active site back to more general ideas about protein structure. The idea of activa ...
... Although the lock and key model is an obvious staging post, ensure that students can distinguish between it and the induced fit model. It is useful to relate the structure of an enzyme and the specificity of the active site back to more general ideas about protein structure. The idea of activa ...
Metabolic network modelling
Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑