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DEPARTMENT OF BIOCHEMISTRY UNIVERSITY OF KERALA
DEPARTMENT OF BIOCHEMISTRY UNIVERSITY OF KERALA

... undergraduate level and basic understanding of the theory and principle of the concerned subject. ...
Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)
Genomic Insights into Methanotrophy: The Complete Genome Sequence of Methylococcus capsulatus (Bath)

... roles in other major parts of the carbon cycle such as photosynthesis, no genome-level studies have been published on the biology of methanotrophs. We report the first complete genome sequence to our knowledge from an obligate methanotroph, Methylococcus capsulatus (Bath), obtained by the shotgun se ...
PrACtICE PArAmEtEr: EvALuAtIoN of thE ChILd wIth mICroCEPhALy
PrACtICE PArAmEtEr: EvALuAtIoN of thE ChILd wIth mICroCEPhALy

... or proportionate microcephaly may go unrecognized unless a child’s HC is measured accurately. Making comparisons to parents’ HCs may be important as familial forms of mild microcephaly have been described. Currently available assessment tools may not ultimately establish a specific etiologic diagnos ...
Role of NAD+-Dependent Malate Dehydrogenase in the Metabolism
Role of NAD+-Dependent Malate Dehydrogenase in the Metabolism

Lab 5
Lab 5

... give a more accurate, and usually higher, activity reading. The first step is the homogenization of beef heart muscle tissue at 4oC. There are several methods for homogenization. In the case of animal tissue which lacks cell walls, a gentle approach can be taken: the tissue can be homogenized using ...
Enzymatic Synthesis of Arginine Phosphate with Coupled ATP
Enzymatic Synthesis of Arginine Phosphate with Coupled ATP

... used both to facilitate recovery and reuse of the enzyme, and to improve its stability under operating condition^.^ Three successive reactions were carried out using the same preparation of enzyme-containing gel. The results were comparable for the three. In the last, it was possible to reach a conc ...
Theoretical questions
Theoretical questions

... practical methods of investigation and achieved in control of theoretical level points number not less then 50. ...
Metabolic adaptation of Mycobacterium avium subsp
Metabolic adaptation of Mycobacterium avium subsp

... Data processing and bioinformatics. Verified datasets were ...
Full-Text PDF
Full-Text PDF

... δ-carotene (ε,ψ-carotene) to produce α-carotene. No ε-cyclization activity was identified for BfLCYB. Sequence comparison showed that BfLCYB shares conserved domains with other functionally characterized lycopene cyclases from different organisms and belongs to a group of ancient lycopene cyclases. ...
High-Fidelity Contrast Reaction Simulation Training: Performance
High-Fidelity Contrast Reaction Simulation Training: Performance

... comfort levels of management of contrast reactions, was created. Three simulation scenarios that represented a moderate reaction, a severe reaction, and a contrast reaction mimic were completed in a one-hour period in a simulation laboratory. All participants completed a pretest and a posttest at on ...
Vitamins and Coenzymes - KSU - Home
Vitamins and Coenzymes - KSU - Home

... • Well known examples include: – Reduction of an alcohol to an alkane catalyzed by ribonucleotide reductase – Carbon chain rearrangements catalyzed by methylmalonyl CoA mutase or glutamate mutase ...
Sites of enzyme activity along the nephron
Sites of enzyme activity along the nephron

Reprint pdf - Sportsci.org
Reprint pdf - Sportsci.org

... The scientific method involves stringent criteria for the evaluation of knowledge, but the method is not perfect. Research findings and their interpretations can be raised prematurely to the status of a fact. Some of these “facts” can even become a pivotal component of a knowledge base, termed a con ...
Chronic Metabolic Acidosis Destroys Pancreas
Chronic Metabolic Acidosis Destroys Pancreas

Fluid & electrolyte Disorders Part 2
Fluid & electrolyte Disorders Part 2

... • Rest of the choices are renal K-wasting states wherein the 24-hour urine K excretion should be > 15mmol/d. • The patient later admitted thru an interpreter that he ate fishballs from a sidewalk vendor 2 days ago and has been having diarrhea since then. ...
8)Discuss the roles of cofactors and coenzymes in enzyme activity.
8)Discuss the roles of cofactors and coenzymes in enzyme activity.

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1 - Plant Research International

... exposed to radioactive intermediates followed by extraction and HPLC analysis to establish where in the cell the different pathways operate and if there is a site of synthesis with a separate site of accumulation. Sulphur Biochemistry Workpackage: Genes for CSO synthesis The genes responsible for th ...
ENZYMES at Lew Port`s Biology Place
ENZYMES at Lew Port`s Biology Place

Frequency, Outcome, and Appropriateness of Treatment of Nonionic
Frequency, Outcome, and Appropriateness of Treatment of Nonionic

... instituted treatment was completed. No patients with adverse reactions consisting entirely of physiologic manifestations were in­ cluded. Each reaction was graded retrospectively by consensus of two authors as mild, moderate, or severe based on the most severe clinical symptom or sign. The classific ...
1 - UCSB C.L.A.S.
1 - UCSB C.L.A.S.

... Trypsin, with an optimum pH at 6-7, will have AA residues at its active site that can be protonated and deprotonated at this pH as so we would expect His. We also know there is Asp and Ser. Pepsin, with an optimum pH at 2-3, will have AA residues at its active site that can be protonated and deproto ...
Enzyme - MACscience
Enzyme - MACscience

... 2. The substrate shape must be compatible with the enzymes active site in order to fit and be reacted upon. 3. The enzyme modifies the substrate. In this instance the substrate is broken down, releasing two products. ...
Report Organelles in Blastocystis that Blur the
Report Organelles in Blastocystis that Blur the

20 February 2002/ 09 February 2007
20 February 2002/ 09 February 2007

... Premature or term infants may retain an excess of sodium due to immature renal function. In premature or term infants, repeated infusions of sodium chloride should therefore only be given after determination of the serum sodium level. Newborns – especially those born premature and with low birth wei ...
A generalized stoichiometric model of C3, C2, C2
A generalized stoichiometric model of C3, C2, C2

... 2013; Singh et  al., 2014). Classical photosynthetic models have allowed the simulation of leaf-level assimilation in C3, C2, C2+C4, and C4 plants using a mechanistic description based on Rubisco PEPC kinetics (von Caemmerer, 1989, 2000, 2013). These models are based on several simplifications, whic ...
brv12140 - Cambridge Repository
brv12140 - Cambridge Repository

... as precursors for aromatic amino acids. By contrast, the oxidative branch of the PPP is not universal and is absent in many aerobic and thermophilic organisms (Grochowski, Xu & White, 2005; Nunoura et al., 2011; Bräsen et al., 2014). While reactions of the non-oxidative branch can also occur non-enz ...
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Metabolic network modelling



Metabolic network reconstruction and simulation allows for an in-depth insight into the molecular mechanisms of a particular organism. In particular, these models correlate the genome with molecular physiology. A reconstruction breaks down metabolic pathways (such as glycolysis and the Citric acid cycle) into their respective reactions and enzymes, and analyzes them within the perspective of the entire network. In simplified terms, a reconstruction collects all of the relevant metabolic information of an organism and compiles it in a mathematical model. Validation and analysis of reconstructions can allow identification of key features of metabolism such as growth yield, resource distribution, network robustness, and gene essentiality. This knowledge can then be applied to create novel biotechnology.In general, the process to build a reconstruction is as follows: Draft a reconstruction Refine the model Convert model into a mathematical/computational representation Evaluate and debug model through experimentation↑
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