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Supplementary Figures
Supplementary Figures

... Supplementary Figure 1: Epsilon15 genome and identified protein products. The genome of Epsilon15 (accession #NC_004775) is 39671 bp and contains 49 predicted open reading frames (orfs). Six Epsilon15 virion structural proteins were matched to orfs by mass spectrometric peptide mapping of trypsin di ...
Reason 6: Protein Manufacture: Ribosomes are proof of
Reason 6: Protein Manufacture: Ribosomes are proof of

... required to build a skyscraper like the proteins to make life. The concrete is a unique protein, the glass on the surface, the steel, the electrical wires, the carpeting, the air conditioning machines, the heating units, the paint on the walls; each unique building element could be compared to a uni ...
protein_web_notes1
protein_web_notes1

... Essential amino acids cannot be made by your body. You must get them from the foods you eat. Non-essential amino acids are the acids your body can make. DNA (deoxyribonucleic acid) is found in every nucleus of a cell. It has the instructions for how amino acids will be linked to form the proteins in ...
Controlling the genes
Controlling the genes

... • Genes can be regulated by both on switches and off switches • Gene repressors turn off or reduce gene expression • Gene activators turn on or enhance gene expression – Read page 273 for a good account – Learn what an operon is here - a set of genes that are transcribed into a single mRNA- question ...
Ch 3 Check Your Progress Answers BC Biology 12 3.1 p 67 1
Ch 3 Check Your Progress Answers BC Biology 12 3.1 p 67 1

... c) cell recognition protein: glycoproteins that help the body recognize self vs others and can help recognize invaders like bacteria d) receptor proteins: have a shape that allows a specific molecule to bind to it. The binding causes the shape of the protein to change and to have a cellular response ...
Plant Transformation
Plant Transformation

... system was devised (Fig B) • In this case, a hygromycin resistance gene was placed under the control of a constitutive promoter next to a promoter less reporter gene within the TDNA • After hygromycin-resistant transformants are selected, the transformants can be checked by an enzyme assay under dif ...
Transport and local translational regulation of mRNAs in neurons
Transport and local translational regulation of mRNAs in neurons

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Name - Schuette Science
Name - Schuette Science

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The novel functions of the cytochrome b561 protein family in
The novel functions of the cytochrome b561 protein family in

... members are studied in detail for their physiological functions but most of them are not well understood. In the present study, we focused on a nematode C. elegans as the most suitable model. C. elegans contains 7 b561 homologs (Ceb561-1 to 7) but their functions are not studied. We chose Ceb561-1 a ...
Regulation of Gene Expression
Regulation of Gene Expression

... Myogenic helix-loop-helix proteins (MyoD, etc.) and skeletal muscle • Trigger becoming muscle cell • Muscle-specific expression • Coordinately activate muscle genes • Specific for muscle genes ...
Welcome to Techniques in Molecular Biology
Welcome to Techniques in Molecular Biology

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Semester Test Practice Test
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Simulating Protein Synthesis and RNA Interference in the
Simulating Protein Synthesis and RNA Interference in the

... 1) Select five student volunteers to represent the active parts of Protein Synthesis 2) One student will represent the DNA Transcription proteins and should be seated at a table on one end of the room 3) A second student will represent the Ribosome, responsible for making proteins from RNA instructi ...
Biology 212 General Genetics
Biology 212 General Genetics

...  Can lead to mental retardation if not treated from birth  Treat by placing babies on diet low in phenylalanine  Screening with routine blood test just after birth  1/8000 among Caucasians in U.S., therefore relatively common Defects in other enzymes of this pathway lead to other diseases. 3. DN ...
Protein Therapeutics
Protein Therapeutics

... With B. subtilis and some others, it is possible to induce secretion of a gene product into the surrounding medium. This method is in use in the pharmaceutical industry in the production of hormones such as insulin and human growth hormone. Disadvantages of bacterial cells The expressed proteins oft ...
What meaning(s) do these two photos represent? (Hint* dna,rna
What meaning(s) do these two photos represent? (Hint* dna,rna

... As the ribosome moves along the mRNA, the tRNA transfers its amino acid to the growing protein chain, producing the protein - codon by codon!  3. Termination: when the ribosomes hits a stop codon UAA, UGA, or UAG - the ribosome falls apart!  The same mRNA may be used hundreds of times during trans ...
From Genes to Proteins
From Genes to Proteins

... Keratin is one of the proteins in hair. The gene for keratin is transcribed and translated by certain skin cells. The series of letters on the next slide represents the sequence of nucleotides in a portion of an mRNA molecule transcribed from the gene for keratin. This mRNA strand and the genetic co ...
Sturctural and functional prediction of shigella
Sturctural and functional prediction of shigella

... • Enzymes : (13). Survival, for they provide nutrients for growth and are responsible for multiplication of the organism. • Hydorolase: Play decisive role in synthesis, lysis, invasion of host cells. These processes are essential for survival, growth and development of living organism. We have ident ...
Introduction to proteomics: analysis of proteins in complex biological
Introduction to proteomics: analysis of proteins in complex biological

... Proteomics & disease analysis, part I: • Characterize protein differences between disease and normal tissues-– For understanding the disease process; – To develop drug targets; ...
Gene Section SMAP1 (stromal membrane-associated protein 1) Atlas of Genetics and Cytogenetics
Gene Section SMAP1 (stromal membrane-associated protein 1) Atlas of Genetics and Cytogenetics

... Schematic illustration of chimeric MLL-SMAP1 protein. The authentic MLL and SMAP1 proteins are also shown. AT hook, an AT-hook domain; NLS, a nuclear localization signal; MT, a methyltransferase domain; PHD, a plant homeodomain zinc finger; BROMO, a bromo domain; SET, a su(var)3-9, enhancer-of-zeste ...
Ass4 - The University of Sydney
Ass4 - The University of Sydney

... Protein kinase C could be activated via both growth factor and 7-TMS receptor signalling Protein kinase C becomes activated after dissociation of its regulatory subunits Phospholipase C hydrolyses phospholipids in the ER membrane, leading to Ca2+ release Diacyl glycerol is phosphorylated and becomes ...
Praktikum Information Integration - HU
Praktikum Information Integration - HU

... protein_version_id), have a status, are on a chromosome, have a start and end position, and a chromosomal location – Gene function: Are described by a taxonomy of terms which forms a DAG; each term has an ID, a name, a description, and can be annotated to multiple genes – Gene – Function relationshi ...
Figure 5.1 Rapid Diffusion of Membrane Proteins The fluid mosaic
Figure 5.1 Rapid Diffusion of Membrane Proteins The fluid mosaic

... examined the movement of proteins within the cell membrane by constructing heterokaryons, cells comprised of nuclei from both mice and humans. By using fluorescent stains (red or green) that were specific either to the mouse or human proteins (antigens), Frye and Edidin observed that after 40 minute ...
B Cell Receptor Complexes - Sigma
B Cell Receptor Complexes - Sigma

... B Cell Receptor Complexes Reversible tyrosine phosphorylation plays an important role in regulating B cell function. In particular, binding of antigen to the B cell receptor (BCR) promotes the activation of several protein tyrosine kinases that, in conjunction with protein tyrosine phosphatases, al ...
Summary for Chapter 6 – Protein: Amino Acids
Summary for Chapter 6 – Protein: Amino Acids

... membranes of intestinal cells transport the amino acids into the cells, where they are released into the bloodstream. Cells synthesize proteins according to the genetic information provided by the DNA in the nucleus of each cell. This information dictates the order in which amino acids must be linke ...
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Protein moonlighting



Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.
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