Datasheet PDF - BioAssay Systems
... Bradford Colorimetric Protein Determination at 595 nm DESCRIPTION The protein is known as the "building blocks of life" and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quan ...
... Bradford Colorimetric Protein Determination at 595 nm DESCRIPTION The protein is known as the "building blocks of life" and is one of the most important macromolecules in life science. Proteins are polypeptides made up of amino acids and play various key roles in all aspects of biology. Protein quan ...
Document
... C. signal sequence synthesis on ribosomes. D. SRP binds signal sequence and subsequently binds SRP-receptor. E. ribosome dissociates. A. A, C, E, B, D B. C, D, A, B, E C. C, A, D, B, E D. A, C, B, D, E E. C, B, D, E, A The ribosome and the _______ form a common conduit for transfer of the nascent pr ...
... C. signal sequence synthesis on ribosomes. D. SRP binds signal sequence and subsequently binds SRP-receptor. E. ribosome dissociates. A. A, C, E, B, D B. C, D, A, B, E C. C, A, D, B, E D. A, C, B, D, E E. C, B, D, E, A The ribosome and the _______ form a common conduit for transfer of the nascent pr ...
No Slide Title
... Result of an MeCP2 blastp search: A methyl-binding domain shared by several proteins ...
... Result of an MeCP2 blastp search: A methyl-binding domain shared by several proteins ...
PART I. TUTORIAL QUESTIONS (30 marks total)
... Also acceptable: usually requires overexpression of the protein of interest. Overexpression may have negative effects on the cell or in the protein’s normal function. 4. What is the purpose of using SDS? (2 marks) It denatures proteins by removing their quaternary, tertiary, and secondary structure ...
... Also acceptable: usually requires overexpression of the protein of interest. Overexpression may have negative effects on the cell or in the protein’s normal function. 4. What is the purpose of using SDS? (2 marks) It denatures proteins by removing their quaternary, tertiary, and secondary structure ...
Protein Building Blocks (PBBs): Toys for teaching the principles of
... as follows: positive blocks have magnets embedded on four sides with all N sides facing out, negative blocks have magnets with all S sides facing out. Partially assembled blocks are shown in Figure 1. Thus, like charged particles repel each other, but unlike charges are attractive. Hydrophobic inter ...
... as follows: positive blocks have magnets embedded on four sides with all N sides facing out, negative blocks have magnets with all S sides facing out. Partially assembled blocks are shown in Figure 1. Thus, like charged particles repel each other, but unlike charges are attractive. Hydrophobic inter ...
Leukaemia Section t(11;14)(p13;q11), t(7;11)(q35;p13) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Result of the chromosomal anomaly ...
... Result of the chromosomal anomaly ...
07fasebabstracts1
... are crucial to identifying the full mitochondrial protein complement (‘mitochondrial proteome’) and elucidating the function and evolution of individual protein components. Previous mitochondrial proteomics efforts have focused on fungi, animals and plants. These studies have neglected the unicellul ...
... are crucial to identifying the full mitochondrial protein complement (‘mitochondrial proteome’) and elucidating the function and evolution of individual protein components. Previous mitochondrial proteomics efforts have focused on fungi, animals and plants. These studies have neglected the unicellul ...
PPT File
... Concept 11.3 Eukaryotic Genes Are Regulated by Transcription Factors and DNA Changes Transcription factors recognize particular nucleotide sequences: NFATs (nuclear factors of activated T cells) are transcription factors that control genes in the immune system. ...
... Concept 11.3 Eukaryotic Genes Are Regulated by Transcription Factors and DNA Changes Transcription factors recognize particular nucleotide sequences: NFATs (nuclear factors of activated T cells) are transcription factors that control genes in the immune system. ...
- Flintbox
... a mutation of the CFTR protein that effectively blocks ion exchange in and out of these cells resulting in the cell’s inability to absorb water. A common symptom of CF is the formation of excess mucus that promotes the ion/water preclusion as well as trapping unwanted bacteria leading to chronic inf ...
... a mutation of the CFTR protein that effectively blocks ion exchange in and out of these cells resulting in the cell’s inability to absorb water. A common symptom of CF is the formation of excess mucus that promotes the ion/water preclusion as well as trapping unwanted bacteria leading to chronic inf ...
Table of nitrogen base
... acids combine to form proteins. In a process known as transcription (takes place in the nucleus) messenger RNA (mRNA) reads and copies the DNA. mRNA then takes this message out of the nucleus into the cytoplasm to the ribosome (rRNA), the site of protein synthesis in a process known as translation. ...
... acids combine to form proteins. In a process known as transcription (takes place in the nucleus) messenger RNA (mRNA) reads and copies the DNA. mRNA then takes this message out of the nucleus into the cytoplasm to the ribosome (rRNA), the site of protein synthesis in a process known as translation. ...
duplicativenetworks
... enzymes, catalysors to chemical reactions of the metabolism components of cellular machinery (e.g. ribosomes) regulators of gene expression ...
... enzymes, catalysors to chemical reactions of the metabolism components of cellular machinery (e.g. ribosomes) regulators of gene expression ...
interpreted as a demonstration of a biologically significant protein
... cold room and the buffer is kept on ice, but just before use it is brought into the fume hood along with a fraction collector. Fractions of 400/xl are collected and placed on ice. A small amount (3 txl) of each fraction is counted to locate the protein peak, these are pooled, the protein concentrati ...
... cold room and the buffer is kept on ice, but just before use it is brought into the fume hood along with a fraction collector. Fractions of 400/xl are collected and placed on ice. A small amount (3 txl) of each fraction is counted to locate the protein peak, these are pooled, the protein concentrati ...
University of Groningen Hyperthermia and protein
... the induction/synthesis of hsp’s nor thermotolerance development seems to be regulated by changes in [Ca2+]i. In the chapters 3-5 two hsp’s were investigated regarding their involvement in the cell’s ability to deal with heat-induced nuclear protein aggregation. Rat-1 cells transfected with human hs ...
... the induction/synthesis of hsp’s nor thermotolerance development seems to be regulated by changes in [Ca2+]i. In the chapters 3-5 two hsp’s were investigated regarding their involvement in the cell’s ability to deal with heat-induced nuclear protein aggregation. Rat-1 cells transfected with human hs ...
SEMESTER II LSM4241 FUNCTIONAL GENOMICS
... SEMESTER II LSM4241 FUNCTIONAL GENOMICS Prerequisite: LSM3231 Workload: 30 lecture hours + 20 tutorial, self study and presentation hours This module aims to introduce selected topics on functional genomics. Areas covered include: the assignment of functions to novel genes following the genome-seque ...
... SEMESTER II LSM4241 FUNCTIONAL GENOMICS Prerequisite: LSM3231 Workload: 30 lecture hours + 20 tutorial, self study and presentation hours This module aims to introduce selected topics on functional genomics. Areas covered include: the assignment of functions to novel genes following the genome-seque ...
Leukaemia Section t(11;21)(q21;q22) Atlas of Genetics and Cytogenetics
... inhibited cell adhesion. Strongly expressed in hematopoietic cells. LPXN is involved in bone resorption and stimulates prostate cancer cell migration (Chen and Kroog, 2010). ...
... inhibited cell adhesion. Strongly expressed in hematopoietic cells. LPXN is involved in bone resorption and stimulates prostate cancer cell migration (Chen and Kroog, 2010). ...
a series of diagrams in larger format.
... Terminator technology consists of three genes. GENE I Gene I is a repressor gene that produces a repressor protein that interacts with a binding site near Gene II. GENE II Gene II is a recombinase gene that is controlled by a promoter. Between the gene and the promoter is a binding site for the repr ...
... Terminator technology consists of three genes. GENE I Gene I is a repressor gene that produces a repressor protein that interacts with a binding site near Gene II. GENE II Gene II is a recombinase gene that is controlled by a promoter. Between the gene and the promoter is a binding site for the repr ...
RNA & Protein Synthesis
... • DNA is found in the nucleus of cells, but proteins are built in the cytoplasm. • DNA cannot leave the nucleus, so a copy of the gene is made in the form of a similar nucleic acid called RNA ...
... • DNA is found in the nucleus of cells, but proteins are built in the cytoplasm. • DNA cannot leave the nucleus, so a copy of the gene is made in the form of a similar nucleic acid called RNA ...
Electrophoresis
... also binding processes with Ka values significantly greater than 107 M-1 can be ...
... also binding processes with Ka values significantly greater than 107 M-1 can be ...
Lecture 8 (9/18/14) Protein Folding
... where malaria is a problem, people's chances of survival actually increase if they carry sickle-cell trait (selection for the heterozygote). ...
... where malaria is a problem, people's chances of survival actually increase if they carry sickle-cell trait (selection for the heterozygote). ...
FST 12 Endoplasmic Reticulum Stress in Human Diseases
... Cell organelles, endoplasmic reticulum (ER) and mitochondria play an important role in the maintenance of cellular homeostasis and dysfunction of either or both have been implicated in many human diseases, including cancer, diabetes, cardiovascular, neurodegeneration and ischemia. The ER is the prim ...
... Cell organelles, endoplasmic reticulum (ER) and mitochondria play an important role in the maintenance of cellular homeostasis and dysfunction of either or both have been implicated in many human diseases, including cancer, diabetes, cardiovascular, neurodegeneration and ischemia. The ER is the prim ...
Table S1.
... by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-depend ...
... by alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. This protein is found to undergo cell cycle-depend ...
lecture 21
... protein unfolding (degradation) - accessories to protease complexes; sometimes they are joined to the protease (i.e., FtsH membrane protease) membrane trafficking - NSF dissociates the SNARE complex, which brings two membranes together to facilitate fusion in vesicle trafficking pathways micro ...
... protein unfolding (degradation) - accessories to protease complexes; sometimes they are joined to the protease (i.e., FtsH membrane protease) membrane trafficking - NSF dissociates the SNARE complex, which brings two membranes together to facilitate fusion in vesicle trafficking pathways micro ...
Figure S1. - BioMed Central
... on domain prediction and the crystal structure of other TLRs (refer to supplementary text). The Nglycosylation sites in our study are shown as blue asterisks. (B) Table of N-glycosylation sites in our Nglycoproteome data. The localization of N-glycosylation sites in the domains of each TLR is indica ...
... on domain prediction and the crystal structure of other TLRs (refer to supplementary text). The Nglycosylation sites in our study are shown as blue asterisks. (B) Table of N-glycosylation sites in our Nglycoproteome data. The localization of N-glycosylation sites in the domains of each TLR is indica ...
Drosophila melanogaster
... Localization of cMyBP-C in transgenic Drosophila melanogaster flight muscle Cardiac myosin-binding protein C (cMyBP-C) is a thick filament protein that is critical for proper cardiac muscle function. cMyBP-C increases the stiffness of the thick filaments and aids in their assembly during development ...
... Localization of cMyBP-C in transgenic Drosophila melanogaster flight muscle Cardiac myosin-binding protein C (cMyBP-C) is a thick filament protein that is critical for proper cardiac muscle function. cMyBP-C increases the stiffness of the thick filaments and aids in their assembly during development ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.