- Wiley Online Library
... Figure 1: Model of Drosophila fragile X mental retardation protein (dFmrp) function in the neuron. dFmrp (yellow hexagon) enters into the nucleus (a) via its NLS and forms a messenger ribonucleoprotein (mRNP) complex (b) by interacting with specific RNA transcripts (red hairpin structure) and protei ...
... Figure 1: Model of Drosophila fragile X mental retardation protein (dFmrp) function in the neuron. dFmrp (yellow hexagon) enters into the nucleus (a) via its NLS and forms a messenger ribonucleoprotein (mRNP) complex (b) by interacting with specific RNA transcripts (red hairpin structure) and protei ...
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... Figure 1: Model of Drosophila fragile X mental retardation protein (dFmrp) function in the neuron. dFmrp (yellow hexagon) enters into the nucleus (a) via its NLS and forms a messenger ribonucleoprotein (mRNP) complex (b) by interacting with specific RNA transcripts (red hairpin structure) and protei ...
... Figure 1: Model of Drosophila fragile X mental retardation protein (dFmrp) function in the neuron. dFmrp (yellow hexagon) enters into the nucleus (a) via its NLS and forms a messenger ribonucleoprotein (mRNP) complex (b) by interacting with specific RNA transcripts (red hairpin structure) and protei ...
Chapter 15: Amines 1. What are the different types of amines? Be
... 4.! What is the primary structure of a protein and what conventions are used for drawing and naming primary structures? Be able to define protein primary structure, explain how primary structures are represented, and draw and name a simple protein structure, given its amino acid sequence. 5.! What ...
... 4.! What is the primary structure of a protein and what conventions are used for drawing and naming primary structures? Be able to define protein primary structure, explain how primary structures are represented, and draw and name a simple protein structure, given its amino acid sequence. 5.! What ...
ProdoNet: identification and visualization of prokaryotic gene
... identify new relationships. A variety of databases supply valuable information on transcription factor binding sites and gene regulation. For prokaryotes, these include databases that focus on a single model organism, as RegulonDB for Escherichia coli (1) or DBTBS for Bacillus subtilis (2). Others c ...
... identify new relationships. A variety of databases supply valuable information on transcription factor binding sites and gene regulation. For prokaryotes, these include databases that focus on a single model organism, as RegulonDB for Escherichia coli (1) or DBTBS for Bacillus subtilis (2). Others c ...
17 - Rutgers Chemistry
... bacterial gene that evolved to protect bacteria against the antibiotic chloramphenicol (CAM). The gene encodes for a protein, chloramphenicol acetyltransferase (CAT), that can add an acetyl group (from acetyl CoA) at one or both of the hydroxyl groups on chloramphenicol. This action prevents chloram ...
... bacterial gene that evolved to protect bacteria against the antibiotic chloramphenicol (CAM). The gene encodes for a protein, chloramphenicol acetyltransferase (CAT), that can add an acetyl group (from acetyl CoA) at one or both of the hydroxyl groups on chloramphenicol. This action prevents chloram ...
Mutational analysis of the early f orespore/mother
... The spoZZGA-phoA fusions were placed under the control of the inducible promoter spac in the plasmid pDG148 (Stragier et al., 1988). l a c 2 fusions to spoZZGA codons 36, 87 and 180 were also constructed and placed under the control of spac. phoA and lacZ fusions to spoZZGA(DA6) were derived from wi ...
... The spoZZGA-phoA fusions were placed under the control of the inducible promoter spac in the plasmid pDG148 (Stragier et al., 1988). l a c 2 fusions to spoZZGA codons 36, 87 and 180 were also constructed and placed under the control of spac. phoA and lacZ fusions to spoZZGA(DA6) were derived from wi ...
Etoposide Phosphate Enhances the Acetylation Level
... 1991). Apart from its central role in translation, eEF1A also is involved in other cellular processes, including nuclear export processes, proteolysis, oncogenic transformation, apoptosis, cell proliferation, and organization of the cytoskeleton (Mateyak and Kinzy, 2010). A protein participating in ...
... 1991). Apart from its central role in translation, eEF1A also is involved in other cellular processes, including nuclear export processes, proteolysis, oncogenic transformation, apoptosis, cell proliferation, and organization of the cytoskeleton (Mateyak and Kinzy, 2010). A protein participating in ...
Regulators Discover Hidden Viral Gene in GMO Crops
... Big Lessons for Biotechnology It is perhaps the most basic assumption in all of risk assessment that the developer of a new product provides regulators with accurate information about what is being assessed. Perhaps the next most basic assumption is that regulators independently verify this informat ...
... Big Lessons for Biotechnology It is perhaps the most basic assumption in all of risk assessment that the developer of a new product provides regulators with accurate information about what is being assessed. Perhaps the next most basic assumption is that regulators independently verify this informat ...
tissue-specificity of storage protein genes has evolved
... synthesis of tissue-specific trans-acting factors in a temporal fashion. It was therefore unexpected that one of the zein genes is strongly expressed in tissue culture. When a chimeric gene controlling expression of the green fluorescence protein (GFP) was introduced into immature cultured embryos b ...
... synthesis of tissue-specific trans-acting factors in a temporal fashion. It was therefore unexpected that one of the zein genes is strongly expressed in tissue culture. When a chimeric gene controlling expression of the green fluorescence protein (GFP) was introduced into immature cultured embryos b ...
26 Fungal Genetics Newsletter Michelle Dequard-Chablat and Philippe Silar
... coded by su1 and su2/AS2 respectively (Gagny et al. 1998), ribosomal proteins S12 coded by AS1 (Dequard-Chablat et al. 1994), S7 coded by su12 (Silar et al. 1997) and S1 coded by su3 (Silar et al. 2003). S12, S7 and S1 refer to the P. anserina numbering for ribosomal proteins (Dequard-Chablat et al. ...
... coded by su1 and su2/AS2 respectively (Gagny et al. 1998), ribosomal proteins S12 coded by AS1 (Dequard-Chablat et al. 1994), S7 coded by su12 (Silar et al. 1997) and S1 coded by su3 (Silar et al. 2003). S12, S7 and S1 refer to the P. anserina numbering for ribosomal proteins (Dequard-Chablat et al. ...
Investigating protein conformation-based inheritance
... NMGR526 fusion protein (¢gure 3b, right). Thus, GdHCl treatment of the yeast cells did not change the expression level of the fusion transcriptional factor. Instead, it induced a heritable change in its activity, probably due to a change in NMGR526 conformation. Another test of the protein conformat ...
... NMGR526 fusion protein (¢gure 3b, right). Thus, GdHCl treatment of the yeast cells did not change the expression level of the fusion transcriptional factor. Instead, it induced a heritable change in its activity, probably due to a change in NMGR526 conformation. Another test of the protein conformat ...
Pvlea-18, a Member of a New Late-Embryogenesis
... The results in Figure 1c show that even 50 mg of GST was unable to block the detection of the 14-kD protein, indicating that the detected protein was not related to GST. Therefore, we can conclude that the antibodies specifically recognized the PvLEA-18 protein. As indicated above, the immunodetecte ...
... The results in Figure 1c show that even 50 mg of GST was unable to block the detection of the 14-kD protein, indicating that the detected protein was not related to GST. Therefore, we can conclude that the antibodies specifically recognized the PvLEA-18 protein. As indicated above, the immunodetecte ...
Heat Shock Proteins
... Heat Sh o ck Pro tein s BPS Bioscience offers a wide range of high purity HSPs for drug discovery research. ...
... Heat Sh o ck Pro tein s BPS Bioscience offers a wide range of high purity HSPs for drug discovery research. ...
T-cell regulator RNF125/TRAC-1 belongs to a novel family of
... isolation. COS-7, HeLa and HEK-293T (human embryonic kidney) cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) (Cambrex), 10 % (v/v) FCS, 100 units/ml penicillin and 0.1 mg/ml streptomycin. For transfection, 107 Jurkat T-cells were electroporated in 250 µl of RPMI 1640 medium containing ...
... isolation. COS-7, HeLa and HEK-293T (human embryonic kidney) cells were grown in DMEM (Dulbecco’s modified Eagle’s medium) (Cambrex), 10 % (v/v) FCS, 100 units/ml penicillin and 0.1 mg/ml streptomycin. For transfection, 107 Jurkat T-cells were electroporated in 250 µl of RPMI 1640 medium containing ...
KOX1, KAP1
... Some transcription factors have, or recruit proteins that have, histone modification and remodeling activities (Fig. 1). Presumably, gene activation requires at least one such factor that can bind its recognition sequence within 'inactive' chromatin and recruit other factors that collaborate in alte ...
... Some transcription factors have, or recruit proteins that have, histone modification and remodeling activities (Fig. 1). Presumably, gene activation requires at least one such factor that can bind its recognition sequence within 'inactive' chromatin and recruit other factors that collaborate in alte ...
ChIP Lysis Buffer High Salt: sc-45001 Material Safety Data Sheet
... Sonication conditions should be optimized since results may vary using different sonifiers. The following conditions were established by using a Sonics VC130 with a 3 mm tip probe. ...
... Sonication conditions should be optimized since results may vary using different sonifiers. The following conditions were established by using a Sonics VC130 with a 3 mm tip probe. ...
The Plasma Membrane: Structure and Function
... • Receptor proteins bind hormones and other substances on the outside of the cell. – Binding triggers a change inside the cell. • Called signal transduction • Example: The binding of insulin to insulin receptors causes the cell to put glucose transport proteins into the membrane. ...
... • Receptor proteins bind hormones and other substances on the outside of the cell. – Binding triggers a change inside the cell. • Called signal transduction • Example: The binding of insulin to insulin receptors causes the cell to put glucose transport proteins into the membrane. ...
1 (a)
... were divided randomly into six groups, with ten patients in each group. 3. Immunoaffinity depletion: Immunoaffinity depletion is a process that is carried out in order to remove the high abundance proteins present in sera, which hamper the process of detection of medium or low abundance tumour deriv ...
... were divided randomly into six groups, with ten patients in each group. 3. Immunoaffinity depletion: Immunoaffinity depletion is a process that is carried out in order to remove the high abundance proteins present in sera, which hamper the process of detection of medium or low abundance tumour deriv ...
How many genes in Arabidopsis come from cyanobacteria? An
... in the first place. Prominent examples of such poorly conserved, but clearly cyanobacterial, proteins in higher plants include the light harvesting complex (LHC) proteins of the thylakoid membrane11 and TOC75, an outer-envelope component of the chloroplast protein-import machinery12, both of which s ...
... in the first place. Prominent examples of such poorly conserved, but clearly cyanobacterial, proteins in higher plants include the light harvesting complex (LHC) proteins of the thylakoid membrane11 and TOC75, an outer-envelope component of the chloroplast protein-import machinery12, both of which s ...
Teacher Notes - 3D Molecular Designs
... • Hydrophobic amino acids will most often be inside proteins. • Hydrophilic amino acids will most often be on the surface of proteins. • Charged amino acids form salt bridges on the surface of proteins. Salt bridges form between oppositely charged amino acids. • Cysteine residues may form disulf ...
... • Hydrophobic amino acids will most often be inside proteins. • Hydrophilic amino acids will most often be on the surface of proteins. • Charged amino acids form salt bridges on the surface of proteins. Salt bridges form between oppositely charged amino acids. • Cysteine residues may form disulf ...
Section 3A Analysis on a Western Blot
... protein, cell in which protein is expressed, tagspecific antibody, secondary antibody). Use the parameters listed in these procedures (antibody concentrations, incubation times, wash conditions, and so forth) only for initial experiments or for determining optimal ranges for each parameter. Pay part ...
... protein, cell in which protein is expressed, tagspecific antibody, secondary antibody). Use the parameters listed in these procedures (antibody concentrations, incubation times, wash conditions, and so forth) only for initial experiments or for determining optimal ranges for each parameter. Pay part ...
Characterization of Tbc2, a nucleus
... Analysis of several nuclear mutants of C. reinhardtii deficient in PSII activity has revealed that each of these mutations identifies a nuclear locus that is required for the translation of the mRNA of one specific PSII subunit (Goldschmidt-Clermont, 1998). For example, translation of the psbC mRNA ...
... Analysis of several nuclear mutants of C. reinhardtii deficient in PSII activity has revealed that each of these mutations identifies a nuclear locus that is required for the translation of the mRNA of one specific PSII subunit (Goldschmidt-Clermont, 1998). For example, translation of the psbC mRNA ...
Protein moonlighting
Protein moonlighting (or gene sharing) is a phenomenon by which a protein can perform more than one function. Ancestral moonlighting proteins originally possessed a single function but through evolution, acquired additional functions. Many proteins that moonlight are enzymes; others are receptors, ion channels or chaperones. The most common primary function of moonlighting proteins is enzymatic catalysis, but these enzymes have acquired secondary non-enzymatic roles. Some examples of functions of moonlighting proteins secondary to catalysis include signal transduction, transcriptional regulation, apoptosis, motility, and structural.Protein moonlighting may occur widely in nature. Protein moonlighting through gene sharing differs from the use of a single gene to generate different proteins by alternative RNA splicing, DNA rearrangement, or post-translational processing. It is also different from multifunctionality of the protein, in which the protein has multiple domains, each serving a different function. Protein moonlighting by gene sharing means that a gene may acquire and maintain a second function without gene duplication and without loss of the primary function. Such genes are under two or more entirely different selective constraints.Various techniques have been used to reveal moonlighting functions in proteins. The detection of a protein in unexpected locations within cells, cell types, or tissues may suggest that a protein has a moonlighting function. Furthermore, sequence or structure homology of a protein may be used to infer both primary function as well as secondary moonlighting functions of a protein.The most well-studied examples of gene sharing are crystallins. These proteins, when expressed at low levels in many tissues function as enzymes, but when expressed at high levels in eye tissue, become densely packed and thus form lenses. While the recognition of gene sharing is relatively recent—the term was coined in 1988, after crystallins in chickens and ducks were found to be identical to separately identified enzymes—recent studies have found many examples throughout the living world. Joram Piatigorsky has suggested that many or all proteins exhibit gene sharing to some extent, and that gene sharing is a key aspect of molecular evolution. The genes encoding crystallins must maintain sequences for catalytic function and transparency maintenance function.Inappropriate moonlighting is a contributing factor in some genetic diseases, and moonlighting provides a possible mechanism by which bacteria may become resistant to antibiotics.