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Poster - University of Waterloo
Poster - University of Waterloo

...  Lactide: Serves as monomer for the hydrophobic block. Polylactide (PLA)based materials are highly biocompatible, biodegradable by enzymes, can hydrolyze under physiological conditions. ...
Slide 1
Slide 1

... • Each protein in a sample responds uniquely in a given protein assay. Such protein-to-protein variation refers to differences in the amount of color (absorbance) obtained when the same mass of various proteins is assayed concurrently by the same method. These differences in color response relate to ...
Enzyme Properties - Illinois Institute of Technology
Enzyme Properties - Illinois Institute of Technology

... Subtilisin prosegment complexed with subtilisin 1.71Å; 35 kDa monomer p. 50 of 52 ...
Scheme of work for Option C, Cells and energy
Scheme of work for Option C, Cells and energy

...  Explain the relationship between action and absorption spectra of photosynthetic pigments  Explain the concept of limiting factors using light intensity, temperature and CO2 concentration as ...
Nucleic Acids
Nucleic Acids

... What Determines Protein Shape? • A protein’s shape is sensitive to the surrounding environment. • Unfavorable temperature and pH changes can cause denaturation of a protein, in which it unravels and loses its shape. • High fevers (above 104º F) in humans can cause some proteins to denature. ...
Techniques
Techniques

... Electrophoresis of membrane proteins • Membrane fragments are solubilized in SDS, which disrupts protein-protein and proteinlipid associations • The proteins are thus coated with negatively charged detergent molecules • The proteins are loaded onto a polyacrylamide gel and an electric potential ap ...
- DigitalCommons@Linfield
- DigitalCommons@Linfield

... water and soaked in colloid solution for 24 hours. ...
(STC) approach with a non selective AFLP fingerprinting
(STC) approach with a non selective AFLP fingerprinting

... PstI/MseI AFLP templates were prepared as described by Vos et al., 1995. 5 μl of the restriction ligation mix was diluted 10 fold in 10 mM Tris-HCl pH 7.5, 0.1 mM EDTA buffer. A non selective amplification with [γ-33]ATP labeled EcoRI+0 and a MseI+0 primers was performed in a total volume of 20 μl. ...
Mitochondrial Uncoupling Proteins in Mammals and Plants
Mitochondrial Uncoupling Proteins in Mammals and Plants

... contains two hydrophobic transmembrane domains. Uncoupling proteins found in mammalian and plant mitochondria (UCPs) are members of this carrier gene superfamily because (1) they have a high sequence homology with other carriers and (2) transport anionic forms of fatty acids or other anions [5]. The ...
Wet Corrosion Conditions for Wet Corrosion Just as we live in an
Wet Corrosion Conditions for Wet Corrosion Just as we live in an

... Electrochemical attack, the scientific name for wet corrosion, depends on three circumstances occurring simultaneously. This might seem restrictive, but the evidence of wet corrosion that we see all around us proves that these conditions are easily met. Rates of mass loss by wet corrosion may be tho ...
Wykład pathology history continues-2015
Wykład pathology history continues-2015

... • Amyloidosis is a group of diseases that are a consequence of abnormal (inappropriately folded) protein deposits. When proteins that are normally soluble in water fold to become amyloids, they become insoluble and deposit in organs • Depending on the structure of the particular amyloid, the protein ...
AUTOMATED DNA SEQUENCING, MegaBACE 1000
AUTOMATED DNA SEQUENCING, MegaBACE 1000

... filled with a separation matrix consisting of a gel and electrode buffer. Two types of gels are used: chemical gels in which the pore structure is dynamic and formed by covalent binding (eg.polyacrylamide) and physical gel entanglement of polymers (as liner polyacrylamide). (Figure 8). Solution phas ...
Inhibiting Biofilm Formation of Pseudomonas Aeruginosa
Inhibiting Biofilm Formation of Pseudomonas Aeruginosa

... project, we investigate a particular type of bacteria, Pseudomonas aeruginosa, which is known for forming proliferative biofilms. The Soong et al. study highlights the importance of a specific enzyme called salivate in the biofilm formation of P. aerations.[2] However, the substrates for the enzyme ...
New high-throughput NMR
New high-throughput NMR

... widths.These problems lead to severe losses in sensitivity, such that NMR experiments are usually conducted at the detection limit, some experts argue. Because of the low sensitivity, GFT spectra will be useful only for ‘well-behaved proteins,’ which are small and highly soluble, says Gottfried Otti ...
Fundamentals of protein structure
Fundamentals of protein structure

... Secondary structures, α-helix and β-sheet, have regular ...
Protein Function and Classification
Protein Function and Classification

... Construction of protein signatures • Construction of a multiple sequence alignment (MSA) from characterised protein sequences. • Modelling the pattern of conserved amino acids at specific positions within a MSA. • Use these models to infer relationships with the characterised sequences ...
40 nm - PLOS
40 nm - PLOS

... Proximity ligation assay (PLA) for signal enhancement and the detection of protein associations in situ Different primary antibodies against Rad51p and Dmc1p or HA and mCherry tags and the corresponding secondary antibodies with attached DNA oligonucleotides were used. Only if different primary anti ...
Isolation, Properties and a Possible Function of a Water
Isolation, Properties and a Possible Function of a Water

... Chl-proteins from these three Brassicaceae plants have molecular masses of 78 to 93 kDa (Murata et al. 1971, Murata and Murata 1971, Murata and Ishikawa 1981). The Lepidium Chl-protein consists of several subunits of 17-20 kDa (Murata 1976, Tabata et al. 1983) and carrys four Chi per protein (Murata ...
Protein Family Analysis: Protein Family Sorter
Protein Family Analysis: Protein Family Sorter

... 1.  To  get  the  data  and  names  on  protein  families,  use  the  mouse  to  draw  a  box  around  the  area   of  interest  in  the  heatmap  (Red  arrow  1  in  Panel  A).    A  pop-­‐up  window  will  appear  that  al ...
A1990CK52000002
A1990CK52000002

... center of gravity has shifted to what we call that the Naturwissenschaften paper has been cited in over 490 publications, making it the molecular biology (including mathematics, physics, chemistry, microbiology, genetics, and most-cited paper for this journal.] even electrotechnics). After it had be ...
A1990CK52000001
A1990CK52000001

... center of gravity has shifted to what we call that the Naturwissenschaften paper has been cited in over 490 publications, making it the molecular biology (including mathematics, physics, chemistry, microbiology, genetics, and most-cited paper for this journal.] even electrotechnics). After it had be ...
A1990CK51900002
A1990CK51900002

... center of gravity has shifted to what we call that the Naturwissenschaften paper has been cited in over 490 publications, making it the molecular biology (including mathematics, physics, chemistry, microbiology, genetics, and most-cited paper for this journal.] even electrotechnics). After it had be ...
Document
Document

... INTRODUCTION Biological molecules and systems have a number of attributes that make them highly suitable for nanotechnology applications. For example, proteins fold into precisely defined three-dimensional shapes, and nucleic acids assemble according to well-understood rules. Antibodies are highly s ...
Anti-Hsp70 antibody [EP1007Y] (HRP)
Anti-Hsp70 antibody [EP1007Y] (HRP)

... IgG ...
Characterization of Rice Group 3 LEA Genes in Developmental
Characterization of Rice Group 3 LEA Genes in Developmental

... centrifugation. Pellets were resuspended in phosphatebuffered saline (150 mM NaCl, 16 mM Na 2 HPO 4 , 4 mM NaH2PO4·2H2O, pH 7.8), and cells were sonicated for 10 min on ice. After centrifugation (18,000 g, 15 min at 4 °C), the supernatant was applied to a nickel-agarose column (Novagen). Purified fu ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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