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Investigating the Dual Function of the Chloride Intracellular Ion
Investigating the Dual Function of the Chloride Intracellular Ion

... I would like to thank my principle supervisor Associate Professor Stella Valenzuela from UTS for her high enthusiasm, dedication and continuous help and support throughout my PhD. Also I would like to express my thankful gratitude to Professor Bruce Cornell from Surgical Diagnostics, Pty, Ltd. I wou ...
Identification of Major Proteins in Maize Egg Cells
Identification of Major Proteins in Maize Egg Cells

... triosephosphate isomerase, which also belongs to the enzymes of the glycolytic pathway. Calculated molecular mass and isoelectric point of cytosolic triosephosphate isomerase (accession number GI136063) are 27,292 and 5.52, respectively. These values fit the position of the gel where spot 5 was dete ...
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File

... (from your own data and those provided) and how you can be confident that this is the case, again referring to the structure of the molecules concerned. Date Due: Task 5 ...
Chapter 5 - macromolecules
Chapter 5 - macromolecules

... What Determines Protein Structure? • The chemical environment affects a protein • Changing pH, salt concentration, temperature, or other environmental factors can cause a protein to unravel • Denature: changing a protein away from its natural shape • A denatured protein is biologically inactive • A ...
Genomics of Theileria parva
Genomics of Theileria parva

... – The effect of a disease or of a therapeutic on RNA and protein levels can be elucidated ...
PDF - 167.13 kbytes - Istituto Superiore di Sanità
PDF - 167.13 kbytes - Istituto Superiore di Sanità

... The LAMP is a molecular biology technique that allows the amplification of specific nucleic acid fragments, which initial and terminal nucleotide sequences are known (oligonucleotide pair). If a species has its own characteristic DNA portion, due to its composition, it is possible to design 2 or 3 p ...
Lecture 9 - Screening cDNA libraries (AMG text pp. 128-134)
Lecture 9 - Screening cDNA libraries (AMG text pp. 128-134)

... library is preferable because rare sequences are present at the same concentration as abundant sequences. Normalized cDNA libraries are constructed by removing abundant cDNA sequences (e.g., actin) using solution hybridization methods. cDNA libraries are commonly screened by either of two different ...
secondary active transport
secondary active transport

... N or C terminal modifications Tightly associates protein to membrane Isoprenylated at C Terminus -Geranylgeranyl and farnesyl groups - from cholesterol ...
A new approach to increase yields and improve
A new approach to increase yields and improve

... growth rate. However, as uncontrolled growth and fast aerobic metabolism deplete oxygen and glucose levels and alter the surrounding pH, bacteria soon switch over to anaerobic metabolism. Uncontrolled nutrient supply, accumulation of harmful metabolites, oxygen depletion and poor pH control limit pr ...
Proteins: Fundamental Chemical Properties
Proteins: Fundamental Chemical Properties

... ionized in solution at neutral physiological pH, with the carboxyl group bearing a negative charge (–COO 2 ) and the amino group a positive charge (–NH31 ). The ionization state varies with pH: in acid solution the carboxyl group is not ionized (–COOH) and the amino group is ionized (–NH31 ). Conver ...
Three Sisters- Beans, Corn and Squash
Three Sisters- Beans, Corn and Squash

... Beans are power-packed sources of protein, fiber and the essential amino acid, lysine. Lysine is missing from most grains, so adding beans to your meal with brown rice gives your body the complete proteins. The amino acids in plant proteins provide us with the ability to build and repair muscles and ...
Srivastava, Sanjay: Analysis of Methods for Predicting Protein Fold and Remote Homologue Recognition
Srivastava, Sanjay: Analysis of Methods for Predicting Protein Fold and Remote Homologue Recognition

... their primary sequences. With this in mind, I chose a trypsin molecule (1TRY) and its sequence homolgs as a starting query sequences for this project. Later I used 3D-PSSM and HMM based SAM-T99 server to predict their 3D structures. It was expected that all the homologs of the starting sequence woul ...
Tagging the Rolling Circle Products with Nanocrystal Clusters for
Tagging the Rolling Circle Products with Nanocrystal Clusters for

... (HRP)-streptavidin conjugate, biotin-14-dATP, Human Brain Total RNA and FluoZin-3 were from Invitrogen (Carlsbad, CA). Super AquaBlue ELISA substrate for HRP color development was ordered from eBioscience Inc. (San Diego, CA).The water used for preparation of all solutions was produced by the MilliQ ...
Protein Structure - Oregon State University
Protein Structure - Oregon State University

... Your secondary structure
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The Chemistry of Living Systems
The Chemistry of Living Systems

... Liquid over a Water exists as a liquid over a large range of temperatures. In fact, wide temperature virtually all living organisms on Earth are most active between the range freezing and boiling point of water. The wide range over which water remains liquid allows most of Earth to be habitable most ...
The mapping of linear B-cell epitope regions in desmoglein 1 and 3
The mapping of linear B-cell epitope regions in desmoglein 1 and 3

... major antigenic site and did not find any difference between normal human sera and PV patient sera using the other peptides. [30]. The aim of the present study is to systematically localize linear B-cell epitope regions of the extracellular parts of Dsg1 (aa50-550) and Dsg3 (aa50-625) proteins recog ...
Template-Directed Synthesis of Structurally Defined Branched
Template-Directed Synthesis of Structurally Defined Branched

... 300 rpm using an Eppendorf thermomixer in standard reaction buffer (300 mM NaCl, 10 mM Tris/Tris HCl pH 7.5, 1 mM EDTA). In this work, side branches are varied to include DNA oligonucleotides and synthetic polymers of different molecular weight. Polyacrylamide Gel Electrophoresis. Gel electrophoresis ...
Supplementary information - Word file (31 KB )
Supplementary information - Word file (31 KB )

... Glutathione-sepharose slurry (600l of 50% in TBST [10mM Tris, pH8.0, 100mM NaCl, 0.2% Tween]) was incubated with GST-Smad4 protein at 4oC for one hour. Beads were precipitated and resuspended in TBST+0.2% BSA, then incubated at 4oC for 10 minutes. This step was repeated and beads were then resuspen ...
Activity series
Activity series

... 1) Create, understand, and use an activity series. 2) Examine redox reactions of metals at a molecular level. Reading: 5.5 p188-192 Group Roles: A Technician; B Leader; C Recorder Redox reactions are some of the most common and most useful chemical reactions. They produce electrical current which ca ...
Protein import into yeast mitochondria van Wilpe, S.
Protein import into yeast mitochondria van Wilpe, S.

... resultingg in the amino acid substitution R to K. Two other timl 7 mutants carried a mutation at eitherr position 100 or position 185, which resulted in the amino acid substitutions G to R and G too D, respectively. The mutations are indicated in the nucleotide sequence of T1M17 and its deducedd pro ...
Defining the inner membrane proteome of E coli
Defining the inner membrane proteome of E coli

... of fusion protein inserted into the membrane ...
Fabrication of Monodisperse, Shape
Fabrication of Monodisperse, Shape

... (MW 68 kDa)), and small anti-cancer agents (doxorubicin) into PEG nanoparticles using the simple, mild and general PRINT technique. We have arguably generated DNA delivery vectors that are themselves first generation “synthetic viruses” (monodisperse populations of shape-specific particles containin ...
sv-lncs - Department of Computer Science and Engineering
sv-lncs - Department of Computer Science and Engineering

... The Minimum Set Cover Problem, MSC, is the problem of finding the minimum size set of sets whose union is equal to the union of all the sets. We approximate the minimum number of domain pairs, which are needed to explain all the protein interactions read from the training data. A protein interaction ...
Master of Science in Biochemistry
Master of Science in Biochemistry

... electrophoresis, and affinity chromatography, gene expression and inter-relationship of genes Applied Molecular Biology Laboratory A series of integrated laboratory exercises with a variety of advanced protein and molecular biology techniques, including mutations within structural genes through the ...
What is a Polymer? - Department of Chemistry
What is a Polymer? - Department of Chemistry

... Amino acids are natural monomers, and polymerize to form proteins. Glucose monomers can also polymerize to form starches, amylopectins and glycogen polymers. In this case the polymerization reaction is known as a dehydration or condensation reaction (due to the formation of water as one of the prod ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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