Sure, some prions can cause diseases, but others are
... prions—despite the fact that their best-known member causes a rare, deadly brain-wasting scourge in humans—can also be good citizens in the life of a cell. In 1982, Stanley Prusiner, a biochemist at the University of California, San Francisco (UCSF), isolated the first prion as the cause of lethal s ...
... prions—despite the fact that their best-known member causes a rare, deadly brain-wasting scourge in humans—can also be good citizens in the life of a cell. In 1982, Stanley Prusiner, a biochemist at the University of California, San Francisco (UCSF), isolated the first prion as the cause of lethal s ...
Algorithms and a Software Application for the Discovery of Heparin
... The first step of this project is a chemical one – examine a group of known heparinbinding proteins and find common subsequences of amino acids that may be an indicator of heparin-binding ability. An algorithm is then developed to search for these sequences given arbitrary proteins as input. The alg ...
... The first step of this project is a chemical one – examine a group of known heparinbinding proteins and find common subsequences of amino acids that may be an indicator of heparin-binding ability. An algorithm is then developed to search for these sequences given arbitrary proteins as input. The alg ...
SUPPLEMENTARY METHODS mRNA isolation and quantitative RT
... for colorectal cancer, at least 10 cm from the tumour (control group) and 7 colonic endoscopic biopsies from patients with a first flare of extensive UC, in order to evaluate the mRNA expression of PPAR, PPAR, NAAA, NAPE-PLD, FAAH and iNOS in the human UC. Colonic samples were divided in the mucos ...
... for colorectal cancer, at least 10 cm from the tumour (control group) and 7 colonic endoscopic biopsies from patients with a first flare of extensive UC, in order to evaluate the mRNA expression of PPAR, PPAR, NAAA, NAPE-PLD, FAAH and iNOS in the human UC. Colonic samples were divided in the mucos ...
2A6
... 【RESULTS AND DISCUSSION】 Figure 1 shows the STM images of the (CH3S)2 molecules on Cu(111) and Ag(111) obtained before and after irradiation with 532 nm (2.3 eV) light. After irradiation, some molecules had broken into two identical ball-shaped protrusions. The S-S bond of a single (CH3S)2 molecule ...
... 【RESULTS AND DISCUSSION】 Figure 1 shows the STM images of the (CH3S)2 molecules on Cu(111) and Ag(111) obtained before and after irradiation with 532 nm (2.3 eV) light. After irradiation, some molecules had broken into two identical ball-shaped protrusions. The S-S bond of a single (CH3S)2 molecule ...
IN VITRO TRANSCRIPTION . TRANSLATION - UTH e
... The most frequently used cell-free translation systems consist of extracts from rabbit reticulocytes and Escherichia coli. All are prepared as crude extracts containing all the macromolecular components (70S or 80S ribosomes, tRNAs, aminoacyl-tRNA synthetases, initiation, elongation and termination ...
... The most frequently used cell-free translation systems consist of extracts from rabbit reticulocytes and Escherichia coli. All are prepared as crude extracts containing all the macromolecular components (70S or 80S ribosomes, tRNAs, aminoacyl-tRNA synthetases, initiation, elongation and termination ...
2.3 Carbon-Based Molecules
... • Proteins are polymers of amino acid monomers. – Twenty different amino acids are used to build proteins in organisms. – Amino acids differ in side groups, or R groups. – Amino acids are linked by peptide bonds. ...
... • Proteins are polymers of amino acid monomers. – Twenty different amino acids are used to build proteins in organisms. – Amino acids differ in side groups, or R groups. – Amino acids are linked by peptide bonds. ...
Chicken eggshell matrix proteins related to anti
... groups according to their characteristics (Table 1). 1. The most abundant egg white proteins have been shown to be localized in the eggshell. Ovalbumin is localized in the mammillae of the eggshell (Hincke 1995) and is predominant at the initial stage of eggshell formation. Lysozyme (Hincke et al., ...
... groups according to their characteristics (Table 1). 1. The most abundant egg white proteins have been shown to be localized in the eggshell. Ovalbumin is localized in the mammillae of the eggshell (Hincke 1995) and is predominant at the initial stage of eggshell formation. Lysozyme (Hincke et al., ...
National Center for Biotechnology Information
... BLAST (Basic Local Alignment Search Tool) provides the functionality of allowing the user to input a protein or DNA sequence and performing a search for structures of similar sequence. Steps for performing a protein BLAST search will be detailed, however a DNA search is very much similar. From th ...
... BLAST (Basic Local Alignment Search Tool) provides the functionality of allowing the user to input a protein or DNA sequence and performing a search for structures of similar sequence. Steps for performing a protein BLAST search will be detailed, however a DNA search is very much similar. From th ...
Bending membranes
... promoted tubule scission and release of coated vesicles. Finally, the clathrin coat surrounding these coated vesicles could be released by the ATP-dependent uncoating reaction mediated by Hsc70 and auxilin. These observations are important, not only because they are the first experiments in which cl ...
... promoted tubule scission and release of coated vesicles. Finally, the clathrin coat surrounding these coated vesicles could be released by the ATP-dependent uncoating reaction mediated by Hsc70 and auxilin. These observations are important, not only because they are the first experiments in which cl ...
function
... The four superfamilies of serine proteases are examples of convergent evolution - Serine proteases fall into several structural superfamilies, which are recognizable from their amino-acid sequences and the particular disposition of the three catalytically important residues in the active site. ...
... The four superfamilies of serine proteases are examples of convergent evolution - Serine proteases fall into several structural superfamilies, which are recognizable from their amino-acid sequences and the particular disposition of the three catalytically important residues in the active site. ...
CD - Instituto de Investigaciones Biotecnológicas
... Radar - De novo repeat detection in protein sequences REP - Searches a protein sequence for repeats REPRO - De novo repeat detection in protein sequences TRUST - De novo repeat detection in protein sequences XSTREAM - De novo tandem repeat detection and architecture modeling in protein sequences SAP ...
... Radar - De novo repeat detection in protein sequences REP - Searches a protein sequence for repeats REPRO - De novo repeat detection in protein sequences TRUST - De novo repeat detection in protein sequences XSTREAM - De novo tandem repeat detection and architecture modeling in protein sequences SAP ...
Flies with disrupted clock (per 01 )
... proteases are known to alter the cellular integrity [5]. Carbonyl formation is an irreversible process and has been closely associated with aging. One of the most important factors controlling the levels of damaged proteins in an organism is the multi-enzymatic proteolytic complex called the proteas ...
... proteases are known to alter the cellular integrity [5]. Carbonyl formation is an irreversible process and has been closely associated with aging. One of the most important factors controlling the levels of damaged proteins in an organism is the multi-enzymatic proteolytic complex called the proteas ...
template
... There are two competing approaches to attack protein-protein docking problem: i) Automated, ii) VR-based. Several research groups are working on automated docking [Morris et al. 1999; Hart and Read 2004] which can be reformulated as an optimization problem. The goal is to minimize binding energy of ...
... There are two competing approaches to attack protein-protein docking problem: i) Automated, ii) VR-based. Several research groups are working on automated docking [Morris et al. 1999; Hart and Read 2004] which can be reformulated as an optimization problem. The goal is to minimize binding energy of ...
Dog and human acid ,-D-galactosidases are structurally similar
... against homogeneous human liver f-galactosidase (A2 plus A3) (Frost et al., 1978) was used to determine cross-reactivity of the dog, cat and human enzymes. Serial dilutions of goat anti-(human f-galactosidase) serum were performed in 10mMphosphate buffer, pH 7.0, containing 0.1 M-NaCl plus 0.02% NaN ...
... against homogeneous human liver f-galactosidase (A2 plus A3) (Frost et al., 1978) was used to determine cross-reactivity of the dog, cat and human enzymes. Serial dilutions of goat anti-(human f-galactosidase) serum were performed in 10mMphosphate buffer, pH 7.0, containing 0.1 M-NaCl plus 0.02% NaN ...
DNA and Protein Concentration Measurements Using Fluorescence
... Three Molecular Probes Quant-iT Assay Kits were used to review the Curie’s performance. The Quant-iT PicoGreen dsDNA Assay Kit (P7589) and the DNA Broad Range DNA Assay Kit (Q33130) were used to measure double-stranded DNA (dsDNA) in solution. The Quant-iT Protein Assay Kit (Q33210) was used to meas ...
... Three Molecular Probes Quant-iT Assay Kits were used to review the Curie’s performance. The Quant-iT PicoGreen dsDNA Assay Kit (P7589) and the DNA Broad Range DNA Assay Kit (Q33130) were used to measure double-stranded DNA (dsDNA) in solution. The Quant-iT Protein Assay Kit (Q33210) was used to meas ...
novel 4E-interacting protein in Leishmania is involved in stage
... the 50 -UTR for scanning by the complex until the first AUG codon is reached. In higher eukaryotes, eIF4G recruits eIF3, that is associated with the 40S ribosomal subunit. eIF4G also interacts with the Poly(A)-Binding Protein (PABP) at the 30 -end of the mRNA, allowing a transient circularization of ...
... the 50 -UTR for scanning by the complex until the first AUG codon is reached. In higher eukaryotes, eIF4G recruits eIF3, that is associated with the 40S ribosomal subunit. eIF4G also interacts with the Poly(A)-Binding Protein (PABP) at the 30 -end of the mRNA, allowing a transient circularization of ...
Post-translational Modifications
... C. Molecular Chaperones: Proteins that mediate correct fate of other polypeptides but are not part of the final structure. Fate includes folding, assembly, interaction with other cellular components, transport, or degradation. A. History: Molecular chaperones initially identified as heat shock prot ...
... C. Molecular Chaperones: Proteins that mediate correct fate of other polypeptides but are not part of the final structure. Fate includes folding, assembly, interaction with other cellular components, transport, or degradation. A. History: Molecular chaperones initially identified as heat shock prot ...
The Plant Cell
... vesicles. Subcellular fractionation and immunolocalization studies confirmed the presence of AtSH3P1 in the endomembrane system. In particular, AtSH3P1 was localized on or adjacent to the plasma membrane and its associated vesicles, vesicles of the trans-Golgi network, and the partially coated retic ...
... vesicles. Subcellular fractionation and immunolocalization studies confirmed the presence of AtSH3P1 in the endomembrane system. In particular, AtSH3P1 was localized on or adjacent to the plasma membrane and its associated vesicles, vesicles of the trans-Golgi network, and the partially coated retic ...
Citrate transporters of Bacillus subtilis Krom, Bastiaan Philip
... which couple translocation to chemical modification of the substrate. Best known are the bacterial phosphotransferase systems that use energy derived from the hydrolysis of phosphoenolpyruvate (PEP) for translocation of sugars. The sugar is phosphorylated during the transport process. Solute transpo ...
... which couple translocation to chemical modification of the substrate. Best known are the bacterial phosphotransferase systems that use energy derived from the hydrolysis of phosphoenolpyruvate (PEP) for translocation of sugars. The sugar is phosphorylated during the transport process. Solute transpo ...
Mass spectrometry and the search for moonlighting proteins
... functions. In some cases, the two functions are very different, as in PHGPx (glutathione peroxidase), a soluble enzyme that is also a sperm structural protein (Ursini et al., 1999). In other proteins, the two functions appear to be more closely related, such as the PMS2 DNA mismatch repair enzyme th ...
... functions. In some cases, the two functions are very different, as in PHGPx (glutathione peroxidase), a soluble enzyme that is also a sperm structural protein (Ursini et al., 1999). In other proteins, the two functions appear to be more closely related, such as the PMS2 DNA mismatch repair enzyme th ...
Chapter 9a - Richsingiser.com
... • Biological membranes may contain as much as 75% to 80% protein or as little as 15-20% protein • Membranes that carry out many enzyme-catalyzed reactions and transport activities are richer in protein • Membranes that carry out fewer such functions (such as myelin sheaths) are richer in lipid • Cel ...
... • Biological membranes may contain as much as 75% to 80% protein or as little as 15-20% protein • Membranes that carry out many enzyme-catalyzed reactions and transport activities are richer in protein • Membranes that carry out fewer such functions (such as myelin sheaths) are richer in lipid • Cel ...
Prediction of mitochondrial proteins of malaria parasite
... evolutionary information was extracted in the form of PSSM profile from multiple alignments of proteins similar to query sequence. We performed PSI-BLAST search of query protein against non-redundant database. Finally a SVM model was developed using composition of PSSM profile which could achieve ma ...
... evolutionary information was extracted in the form of PSSM profile from multiple alignments of proteins similar to query sequence. We performed PSI-BLAST search of query protein against non-redundant database. Finally a SVM model was developed using composition of PSSM profile which could achieve ma ...