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The Exocytic/Lysosomal Transport Pathway
The Exocytic/Lysosomal Transport Pathway

... Directed Translocation of Secretory Vesicles • apical targeting in caveolin coated, lipidraft vesicles • basolateral targeting signals – direct protein to this membrane ...
MTC15 - toddgreen
MTC15 - toddgreen

... Starch is an α-D-glucose polysaccharide used for energy storage and has two different forms: amylose and amylopectin (roughly 1:3 ratio) Amylose is linear and joined by 1α-4 linkages Amylopectin is branched with branches forming 1α-6 linkages Glycogen is another branched polysaccharide (with similar ...
投影片 1
投影片 1

... each other determine the vibrational spectrum of a molecule. High background of water. Often ...
Principles of Skeletal Muscle Adaptation
Principles of Skeletal Muscle Adaptation

... – also repress gene for fast II b myosin HC, turn on fast IIa myosin HC – not only enlarged, but change in contractile phenotype – larger, slower contracting fiber. ...
The Function of Chloroplast Ribosomes Effects of a
The Function of Chloroplast Ribosomes Effects of a

... Chloroplasts were isolated by the rapid method of Ramirez et al. (1968) from 7-10-day-old pea plants (Pisum sativum) and incubated for 40min at 20°C with either [35S]methionine or [41C]leucine. Red light was used as energy source in the absence of either added ATP or catalysts of photophosphorylatio ...
A genetically programmable protein module as
A genetically programmable protein module as

... Divya Sivaraman, Payal Biswas, and Wilfred Chen Department of Chemical and Environmental Engineering, University of California, Riverside, CA, 92521 ...
Ch 3 Answers to Applying and Data Questions
Ch 3 Answers to Applying and Data Questions

... Figure 3.10 Primary Structure Specifies Tertiary Structure A. Disulfide bonds began forming almost immediately after oxidation began. B. Enzyme activity began appearing 100 minutes after oxidation began. C. Disulfide bonds are necessary for protein tertiary structure and must form before the enzyme ...
E._coli_Topo_IV
E._coli_Topo_IV

... – Catalysis of a DNA topological transformation by transiently cleaving a pair of complementary DNA strands to form a gate through which a second double-stranded DNA segment is passed, after which the severed strands in the first DNA segment are rejoined; changes the linking number in multiples of 2 ...
cDNA Micoroarray Data Analysis
cDNA Micoroarray Data Analysis

... structuring free-text. Such steps involve part of speech tagging, noun and verb phrase chunking and shallow parsing. • Relationship Extractor Module: Build an extractor system using machine-learning techniques, such as ILP, for learning rules that combine the medical ontologies with learned patterns ...
ATP
ATP

... • Synthesis – building bigger molecules from smaller molecules ...
Role of cystinosin in vesicular trafficking and membrane fusion
Role of cystinosin in vesicular trafficking and membrane fusion

... structures and a diminution of the usual pattern of small discrete intracytoplasmic vesicles characteristic of lysosomes. The number of these structures was drastically decreased when cystinosin C-terminal tail, its 5th inter-TM loop, or both motifs were altered. The enlarged lysosomes are reminisce ...
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as PDF

... Agarose gel electrophoresis is commonly used to resolve circular DNA with different supercoiling topology, and to resolve fragments that differ due to DNA synthesis. DNA damage due to increased cross-linking proportionally reduces electrophoretic DNA migration (Blasiak et al., 2000; Lu & Morimoto, 2 ...
Extrapolating Anfinsen`s conclusions…
Extrapolating Anfinsen`s conclusions…

... of catalytic RNA molecules, termed ribozymes (one example of which is the ribosome), have recently been characterised. This observation ie that not all the "doing" molecules in cells are proteins once again raised the issue of the basis of early life. Could RNA-only organisms have preceded the evolu ...
Notes #8 PPT - Duplin County Schools
Notes #8 PPT - Duplin County Schools

... • Must submit test corrections within one week (due next Thursday, February 23) • Tell me the correct answer and WHY it’s the correct answer • Schedule retest whenever you want (before school, lunch and learn, after school, 1st period) • You keep higher score ...
Applied and Environmental Microbiology
Applied and Environmental Microbiology

... BpHNL, PsmHNL-long, and PsmHNL-short cloned into the pEamTA vector were overexpressed in Escherichia coli TOP10F= by induction with 0.1 mM isopropyl-␤-D-thiogalactopyranoside (IPTG) at an optical density (OD) at 600 nm of 0.8 for 20 h at 21°C. Following cell disruption and centrifugation, SDSpolyac ...
GPI Anchor
GPI Anchor

... 2. GPI assembly takes place entirely on the cytoplasmic side of the ER and followed by its translocation to the lumenal side, where attachment to the protein takes place. 3. The transamidase reaction is carried out by a multiprotein complex that has as yet not been isolated in its intact form. 4. Th ...
Poster
Poster

... examples from the mouse experiment. Boxes C and E are enlarged from Box A to show portions of the hippocampus (a part of the brain found in the temporal lobe concerned with memory) of the control mouse. Boxes D and F show the enlarged portions of the hippocampus of the mouse that over expressed the ...
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis

... Agarose gel electrophoresis is commonly used to resolve circular DNA with different supercoiling topology, and to resolve fragments that differ due to DNA synthesis. DNA damage due to increased cross-linking proportionally reduces electrophoretic DNA migration (Blasiak et al., 2000; Lu & Morimoto, 2 ...
Biuret test - WordPress.com
Biuret test - WordPress.com

... charged. Neutralization of this charge bring proteins to the isoelectric point. At this point, maximum precipitation of proteins take place and the protein particles bear zero net charge. Each protein has its own isoelectric point, since they may be precipitating by providing the positively charged ...
Whole Foods Production NS430
Whole Foods Production NS430

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Poster - Protein Information Resource
Poster - Protein Information Resource

... scientific community with proteomic information about NIAID's Category A-C biodefense organisms. ...
Chapter 2b
Chapter 2b

... • Increasing [H+], increases acidity. • Increasing [OH] increases alkalinity. • Most organisms grow best between pH 6.5 and 8.5. ...
Biochemistry 462a - Proteins: Primary Sequence
Biochemistry 462a - Proteins: Primary Sequence

... In electrospray ionization (ESI) ions are created by applying a potential to a flowing liquid. This causes the liquid to spray and protein ions to be created. This method can also be used to measure molecular weight, but is most powerful when used in tandem MS/MS. ...
ISOLATION OF A BASIC LECTIN FROM SARGASSUM
ISOLATION OF A BASIC LECTIN FROM SARGASSUM

... Brazilian coast. Lectins are proteins or glycoproteins which interact with carbohydrates through their binding sites. The aim of this work was the characterization of S. cymosum hemagglutinating activity (HA) and isolation of algae lectin (ScyAL) by affinity chromatography. Extract (10%, w/v) was pr ...
Chapter 3 Topic: Biomolecules Main concepts: •In chemistry, the
Chapter 3 Topic: Biomolecules Main concepts: •In chemistry, the

... when the helix or pleated sheet folds back on itself to form twisted or knot-like structures. This is caused by hydrogen bonding, and special bonds called disulfide bridges that form between amino acids. Quaternary structure is where multiple proteins are linked together into one large structure. He ...
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QPNC-PAGE

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This standardized variant of native gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins or protein isoforms in complex protein mixtures.
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