Diversity in biological molecules
... Websites) can be used to line up a series of protein sequences to show which ones are most similar and, interestingly, whether the sequence of some parts of the protein is more highly conserved across species than other parts. Although an interested non-expert (including a school pupil) can carry ou ...
... Websites) can be used to line up a series of protein sequences to show which ones are most similar and, interestingly, whether the sequence of some parts of the protein is more highly conserved across species than other parts. Although an interested non-expert (including a school pupil) can carry ou ...
Protein structure and Function
... Serine and threonine: Each contains a polar hydroxyl group that can participate in hydrogen bond formation. Additionally this polar hydroxyl group can serve as a site of attachment of structure such as phosphate group or an important component of active site of many enzymes. Asparagine and glutamine ...
... Serine and threonine: Each contains a polar hydroxyl group that can participate in hydrogen bond formation. Additionally this polar hydroxyl group can serve as a site of attachment of structure such as phosphate group or an important component of active site of many enzymes. Asparagine and glutamine ...
DNA REPAIR AND CANCER: ROLE OF THE BREAST
... is the tool of choice for high-resolution studies. Nevertheless, big proteins are more difficult to crystallise. So, these studies tend to be restricted to the analysis of smaller domains within the big protein. The structural studies of large macromolecular assemblies where different proteins inter ...
... is the tool of choice for high-resolution studies. Nevertheless, big proteins are more difficult to crystallise. So, these studies tend to be restricted to the analysis of smaller domains within the big protein. The structural studies of large macromolecular assemblies where different proteins inter ...
Protein
... Heat is the most common agent in denaturing protein. The temperature is significant, the degree of denaturation increase 600 times for every 10°C. ...
... Heat is the most common agent in denaturing protein. The temperature is significant, the degree of denaturation increase 600 times for every 10°C. ...
Enzymes
... What would happen if you boil it? Guesses -____denature________ What would happen if you put it in acid? _______denature_________ What would happen in the cold?________inhibit________ ...
... What would happen if you boil it? Guesses -____denature________ What would happen if you put it in acid? _______denature_________ What would happen in the cold?________inhibit________ ...
RIBOSOMES
... 23S,16S & 5S rRNA are transcribed by specific segments of nucleoid. 23S & 5S rRNA associates with cytoplasmic proteins to form 50S subunit. ...
... 23S,16S & 5S rRNA are transcribed by specific segments of nucleoid. 23S & 5S rRNA associates with cytoplasmic proteins to form 50S subunit. ...
FUNCTIONS OF CELL ORGANELLES
... Some residual bodies in non dividing cells contain a high amount of a pigmented substance called ...
... Some residual bodies in non dividing cells contain a high amount of a pigmented substance called ...
BCM 6200 - Purification des proteines membranaires
... Better machinery for folding mammalian proteins. Yields / L are generally high for proteins that express. Affinity tags can be used (metal affinity, anti-body affinity, strepatividin, GST, etc.) Easily ruptured (homogenization or sonication) Cons: Cost is significantly higher Do not contain choleste ...
... Better machinery for folding mammalian proteins. Yields / L are generally high for proteins that express. Affinity tags can be used (metal affinity, anti-body affinity, strepatividin, GST, etc.) Easily ruptured (homogenization or sonication) Cons: Cost is significantly higher Do not contain choleste ...
Proteins
... Amino Acids • The amino acids obtained from protein hydrolysis are α-amino acids. • That is, the amino group is on the α-carbon atom, the one adjacent to the carboxyl group. ...
... Amino Acids • The amino acids obtained from protein hydrolysis are α-amino acids. • That is, the amino group is on the α-carbon atom, the one adjacent to the carboxyl group. ...
Slide 1
... Akinetes differentiate upon P limitation but not under N, DIN, Fe, trace elements or light limitation. ...
... Akinetes differentiate upon P limitation but not under N, DIN, Fe, trace elements or light limitation. ...
SECTION 2 - CELL FUNCTION AND BIOCHEMICAL MEASUREMENT
... 11. Plasma proteins contribute to the colloid osmotic pressure of the blood, which is needed for fluid balance. Plasma proteins also serve as circulating enzymes, hormones, transport molecules, and antibodies. Although the liver is a major source of plasma proteins, these molecules can originate fro ...
... 11. Plasma proteins contribute to the colloid osmotic pressure of the blood, which is needed for fluid balance. Plasma proteins also serve as circulating enzymes, hormones, transport molecules, and antibodies. Although the liver is a major source of plasma proteins, these molecules can originate fro ...
Bio-201-chapter-5-MEC
... • Enzymes that digest starch by hydrolyzing linkages can’t hydrolyze linkages in cellulose • Cellulose in human food passes through the digestive tract as insoluble fiber • Some microbes use enzymes to digest cellulose • Many herbivores, from cows to termites, have symbiotic relationships with ...
... • Enzymes that digest starch by hydrolyzing linkages can’t hydrolyze linkages in cellulose • Cellulose in human food passes through the digestive tract as insoluble fiber • Some microbes use enzymes to digest cellulose • Many herbivores, from cows to termites, have symbiotic relationships with ...
2012-ISB-symposium
... We present a visualization and analysis tool, called Spaghetti, for the exploration of mass spectrometry detected peptides and their structural locations. Studying patterns of peptide location across a protein can be used for many purposes: exploring PTM (post translational modification) locations w ...
... We present a visualization and analysis tool, called Spaghetti, for the exploration of mass spectrometry detected peptides and their structural locations. Studying patterns of peptide location across a protein can be used for many purposes: exploring PTM (post translational modification) locations w ...
How Enzymes Work
... reaction. As originally formulated, the structure of the enzyme was proposed to favor atomic vibrations along the reaction coordinate while disfavoring those that would not lead to productive bond-making or bondbreaking steps (18). Recent evidence from different enzyme systems suggests that this fac ...
... reaction. As originally formulated, the structure of the enzyme was proposed to favor atomic vibrations along the reaction coordinate while disfavoring those that would not lead to productive bond-making or bondbreaking steps (18). Recent evidence from different enzyme systems suggests that this fac ...
Molecules, Genes, and Diseases Session 2 Protein Structure and
... • The polypeptide backbone forms regular arrangements of amino acids that are located near to each other in the linear sequence. • These arrangements are termed the secondary structure of the polypeptide . • The α-helix, β-sheet , and β-bend are examples o f secondary structures. • Collagen helix , ...
... • The polypeptide backbone forms regular arrangements of amino acids that are located near to each other in the linear sequence. • These arrangements are termed the secondary structure of the polypeptide . • The α-helix, β-sheet , and β-bend are examples o f secondary structures. • Collagen helix , ...
Influenza A H3N2 (A/Perth/16/2009) Hemagglutinin / HA
... The recombinant hemagglutinin of Influenza A virus (A/Perth/16/2009 (H3N2)) consists of 514 amino acids and predicts a molecular mass of 58 kDa. ...
... The recombinant hemagglutinin of Influenza A virus (A/Perth/16/2009 (H3N2)) consists of 514 amino acids and predicts a molecular mass of 58 kDa. ...
Modelling interactomes
... In sum, we can predict functions for more than 50% of a proteome, approximately ten million protein-protein and protein-DNA interactions with an expected accuracy of 50%. Utility in identifying function, essential proteins, and host pathogen interactions ...
... In sum, we can predict functions for more than 50% of a proteome, approximately ten million protein-protein and protein-DNA interactions with an expected accuracy of 50%. Utility in identifying function, essential proteins, and host pathogen interactions ...
What makes up our blood?
... • RED BLOOD CELLS (Erythrocytes) – The most abundant cells in our blood; they are produced in the bone marrow and contain a protein called hemoglobin that carries oxygen to our cells. • WHITE BLOOD CELLS (Leukocytes) – They are part of the immune system and destroy infectious agents called ...
... • RED BLOOD CELLS (Erythrocytes) – The most abundant cells in our blood; they are produced in the bone marrow and contain a protein called hemoglobin that carries oxygen to our cells. • WHITE BLOOD CELLS (Leukocytes) – They are part of the immune system and destroy infectious agents called ...
Seminal Proteins
... fluid positively charged fractions were incubated with exogenous DNA, it was mistakenly demonstrated that a sort of band substitution was identified. This was occurred when only DNA binding stain (ethidium bromide) was used. But another conclusion was obtained when both DNA – protein binding stain ( ...
... fluid positively charged fractions were incubated with exogenous DNA, it was mistakenly demonstrated that a sort of band substitution was identified. This was occurred when only DNA binding stain (ethidium bromide) was used. But another conclusion was obtained when both DNA – protein binding stain ( ...
Mass spectrometry - 123seminarsonly.com
... 1. The level of transcription of a gene gives only a rough estimate of its level of expression into a protein. An mRNA produced in abundance may be degraded rapidly or translated inefficiently, resulting in a small amount of protein. 2. Many proteins experience post-translational modifications that ...
... 1. The level of transcription of a gene gives only a rough estimate of its level of expression into a protein. An mRNA produced in abundance may be degraded rapidly or translated inefficiently, resulting in a small amount of protein. 2. Many proteins experience post-translational modifications that ...
Agarose gel electrophoresis
... - Separate molecules according to their isoelectric point (pI) - At isoelectric point (pI) molecule has no charge (q=0), hence molecule ceases - pH gradient medium ...
... - Separate molecules according to their isoelectric point (pI) - At isoelectric point (pI) molecule has no charge (q=0), hence molecule ceases - pH gradient medium ...
IL-1β +3953 C/T
... Detection of SNP rs1143634 IL-1β +3953C/T ELFO • technique used for the separation of DNA, RNA, or protein molecules using an electric field applied to a gel matrix • Ethidium bromide (EtBr) an intercalating agent is commonly used as a fluorescent tag • Fragments of linear DNA migrate through agaro ...
... Detection of SNP rs1143634 IL-1β +3953C/T ELFO • technique used for the separation of DNA, RNA, or protein molecules using an electric field applied to a gel matrix • Ethidium bromide (EtBr) an intercalating agent is commonly used as a fluorescent tag • Fragments of linear DNA migrate through agaro ...
Protein purification protocol by Dr. Samina Hyder Haq
... (arginine, histidine, arginine and lysine), the acidic amino acids (aspartate and glutamate) and the neutral hydrophilic amino acids (asparagine, glutamine, serine, threonine, tyrosine and cysteine). Any compound that interferes with these interactions between amino acid side-chains and water, by ...
... (arginine, histidine, arginine and lysine), the acidic amino acids (aspartate and glutamate) and the neutral hydrophilic amino acids (asparagine, glutamine, serine, threonine, tyrosine and cysteine). Any compound that interferes with these interactions between amino acid side-chains and water, by ...
ETimminsSchiffman_ConsPhys 961KB Feb 13 2013
... As technological advances have continued to increase accessibility of whole ...
... As technological advances have continued to increase accessibility of whole ...
Lipid modification of proteins and its relevance to protein targeting
... Four types of lipid modification of eukaryotic proteins have now been recognised (Fig. 1) and others may be yet undiscovered. In total cell lysates from a variety of cells, between ten and fifty proteins of each type can be detected by metabolic labelling and one-dimensional SDS-PAGE analysis (A. I. ...
... Four types of lipid modification of eukaryotic proteins have now been recognised (Fig. 1) and others may be yet undiscovered. In total cell lysates from a variety of cells, between ten and fifty proteins of each type can be detected by metabolic labelling and one-dimensional SDS-PAGE analysis (A. I. ...