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Digestion processes
Digestion processes

... 2 Digestion of fat by the pancreatic enzyme lipase yields free fatty acids and glycerol, still associated with bile salts Fatty acids and bile salts 3 Fatty acids and glycerol diffuse into epithelial cells. There they are recombined and packaged with other lipids and proteins to form chylomicrons. 4 ...
StranDisplace™ II Thermostable DNA Polymerase, 8
StranDisplace™ II Thermostable DNA Polymerase, 8

... Refer to the public articles for recommendations and protocols. Recommended use is 8 units per reaction. Recommended temperature for isothermal amplification is 60-65⁰C. The enzyme is heat inactivated by incubation at 80°C for 10 minutes. Use of this enzyme in certain applications may be covered by ...
Biochemistry/Proteins/Introduction
Biochemistry/Proteins/Introduction

... Proteins can fold into domains A significant number of proteins, especially large proteins, have a structure divided into several independent domains. These domains can often perform specific functions in a protein. For example, a cell membrane receptor might have an extracellular domain to bind a t ...
2 H
2 H

... TSI — Three Sugars and Iron • Three sugars – Glucose (limiting) – Sucrose – Lactose ...
amino acids
amino acids

... • Because a molecule of water (Mr 18) is removed to create each peptide bond, the average molecular weight of an amino acid residue in a protein is about 128 -18 = 110. ...
Bio301 Biochemistry I
Bio301 Biochemistry I

... Where ci is the molar concentration of the ith ionic species and Zi is its ionic charge. At high ionic strengths the solubilities ofproteins as well as those of most other substances, decrease. This effect is known as salting out. You have given 1.0 M solutions of NaCl, (NH4) 2SO4 and K3PO4.In which ...
Transcription & Translation
Transcription & Translation

... Types of RNA 1. mRNA carries the genetic “message” from the nucleus to the cytosol 2. rRNA is the major component of ribosomes 3. tRNA carries specific amino acids, helping to form polypeptides (proteins) ...
Protein Synthesis:
Protein Synthesis:

... starts coding for the protein. This codon is most commonly an AUG. In eukaryotes amino acid encoded by the start codon is methionine. In bacteria, the protein starts instead with the modified amino acid Nformyl methionine (f-Met). In f-Met, the amino group has been blocked by a formyl group to form ...
Title - Iowa State University
Title - Iowa State University

... a. A regulatory molecule binds at a location other than the active site and changes the shape of the enzyme in a way that makes the active site available to the enzyme’s natural substrates. b. Regulatory molecules that are similar in size and shape to the enzyme’s natural substrate inhibits catalysi ...
Protein Synthesis:
Protein Synthesis:

... starts coding for the protein. This codon is most commonly an AUG. In eukaryotes amino acid encoded by the start codon is methionine. In bacteria, the protein starts instead with the modified amino acid Nformyl methionine (f-Met). In f-Met, the amino group has been blocked by a formyl group to form ...
2.Carbohydrates - Distance Education Chennai
2.Carbohydrates - Distance Education Chennai

... follows two different pathways. Pyrimidines are synthesized first from aspartate and carbamoyl-phosphate in the cytoplasm to the common precursor ring structure orotic acid, onto which a phosphorylated ribosyl unit is covalently linked. Purines, however, are first synthesized from the sugar template ...
Nutrition
Nutrition

DNA
DNA

... a. Complete the base sequence of the complementary strand of the hypothetical DNA molecule diagrammed below. b. Use dashed lines to indicate hydrogen bonding between paired bases. c. Show how this molecule would be replicated: o Draw the molecule partially “unzipped” while undergoing replication, fo ...
Teaching Notes
Teaching Notes

... The HIV-1 protease functions as a dimer. The active sites of both the polymer chains are located in the binding pocket where the inhibitor is bound. The polymer chain C closely resembles one of the substrates of this enzyme. ...
Macromolecules and Enzymes final draft
Macromolecules and Enzymes final draft

... c. It causes particles to break apart, which makes it easier for chemical reactions to occur. d. It increases the number of particles that have enough kinetic energy to react when they collide. ...
Chapter 1
Chapter 1

... – Each amide H and carbonyl O is involved in H bonds locking the helix in place – Carbonyl O links to amide H 4 amino acids away – H bonds are parallel to the long axis of the helix – Helix is right-handed – Repeat distance or pitch is 5.4 angstroms – 3.6 amino acids per turn ...
Proteins and amino acids
Proteins and amino acids

Chapter 1 Notes - Potosi School District
Chapter 1 Notes - Potosi School District

... which has the start codon AUG to code for the amino acid methionine 3.Promoters mark the beginning of a DNA chain in prokaryotes, but mark the beginning of 1 to several related genes in eukaryotes 4.The 2 DNA strands separate, but only one will serve as the template & be copied 5.Free nucleotides ar ...
DNA Replication and Protein Synthesis PPT
DNA Replication and Protein Synthesis PPT

... which has the start codon AUG to code for the amino acid methionine 3.Promoters mark the beginning of a DNA chain in prokaryotes, but mark the beginning of 1 to several related genes in eukaryotes 4.The 2 DNA strands separate, but only one will serve as the template & be copied 5.Free nucleotides ar ...
Lecture 13 - 14 Conformation of proteins Conformation of a protein
Lecture 13 - 14 Conformation of proteins Conformation of a protein

...  Primary structure of protein refers to the number of amino acids and the order in which they are covalently linked together.  It also refers to the location of disulfide bridges, if there are any, in a polypeptide chain.  The peptide bond is covalent in nature, quiet stable and referred as backb ...
Polar amino acids with negative charge
Polar amino acids with negative charge

10C Cellular respiration worksheet
10C Cellular respiration worksheet

... 8. How many nucleotides are needed to code for a protein with 450 amino acids? A) at least 150 B) at least 300 C) at least 450 D) at least 900 E) at least 1,350 9. Which of the following is NOT associated with RNA? A) ribose B) thymine C) uracil D) phosphates E) single-strandedness 10. Which of the ...
SP12+ P12 (1+2) Urease: determination of inhibitor
SP12+ P12 (1+2) Urease: determination of inhibitor

SP12+ P12 (1+2) Urease: determination of inhibitor
SP12+ P12 (1+2) Urease: determination of inhibitor

Insulin-Containing Amino Acids and Oligopeptides/β
Insulin-Containing Amino Acids and Oligopeptides/β

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Biosynthesis



Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.
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