gfp_exercise_ver5
... 5 Using the cDNA sequence provided in this exercise, you estimate GFP’s mRNA length. In your laboratory, you then isolate total GFP RNA from jellyfish and resolve it on a gel based on the RNA size difference. You find two different GFP RNAs: one of the RNAs is bigger than your estimate and ...
... 5 Using the cDNA sequence provided in this exercise, you estimate GFP’s mRNA length. In your laboratory, you then isolate total GFP RNA from jellyfish and resolve it on a gel based on the RNA size difference. You find two different GFP RNAs: one of the RNAs is bigger than your estimate and ...
Protocol
... a. gel electrophoresis to analyze the RT-PCR products Part I: Oocyte RNA with Stratagene’s “Absolutely RNA Miniprep Kit” The objective of this part of the experiment is to isolate high quality RNA, which means RNA that is not degraded and is free from DNA contamination. The Stratagene kit uses a spi ...
... a. gel electrophoresis to analyze the RT-PCR products Part I: Oocyte RNA with Stratagene’s “Absolutely RNA Miniprep Kit” The objective of this part of the experiment is to isolate high quality RNA, which means RNA that is not degraded and is free from DNA contamination. The Stratagene kit uses a spi ...
Colorimetric End-Point Determination
... At normal body temperature, cellular chemical reactions, particularly those for the oxidation or transformation of organic compounds would occur very slowly. While raising the temperature would increase the speed of the reaction, living cells cannot be subjected to high temperatures because of the d ...
... At normal body temperature, cellular chemical reactions, particularly those for the oxidation or transformation of organic compounds would occur very slowly. While raising the temperature would increase the speed of the reaction, living cells cannot be subjected to high temperatures because of the d ...
Lecture 9: Citric Acid Cycle/Fatty Acid Catabolism
... LIPID CATABOLISM We have a large amount of fat in our body, ~15 kg of fat, compared with only ~150 g of glycogen. Fat can sustain humans for weeks. Glycogen lasts hours or about a day. Fats (or triglycerides) are an important for long term energy storage. ...
... LIPID CATABOLISM We have a large amount of fat in our body, ~15 kg of fat, compared with only ~150 g of glycogen. Fat can sustain humans for weeks. Glycogen lasts hours or about a day. Fats (or triglycerides) are an important for long term energy storage. ...
DECISION of 28 June 2005
... needs to be investigated. 7. In the application as filed (pages 10 and 11), GDF-9 is described as a 441 amino acids long protein having a Cterminal domain preceded by a putative tetrabasic proteolytic processing site. Yet, it does not exhibit the most striking structural feature which serves to esta ...
... needs to be investigated. 7. In the application as filed (pages 10 and 11), GDF-9 is described as a 441 amino acids long protein having a Cterminal domain preceded by a putative tetrabasic proteolytic processing site. Yet, it does not exhibit the most striking structural feature which serves to esta ...
Lab12
... Halo around the streak (clearing of lipids) = complete hydrolysis of triglycerides, positive for lipase production (agar could be pale blue due to complete clearing of all fatty acids or dark blue because a few fatty acids remain) Pale blue agar, no halo = tributyrin present, negative for lipase pro ...
... Halo around the streak (clearing of lipids) = complete hydrolysis of triglycerides, positive for lipase production (agar could be pale blue due to complete clearing of all fatty acids or dark blue because a few fatty acids remain) Pale blue agar, no halo = tributyrin present, negative for lipase pro ...
Chapter 3: Organic Molecules and life
... Lipids- Non-polar, hydrophobic Proteins Nucleic Acids- DNA & RNA 1. Made of monomers called nucleotides; The nucleotides are made of phosphate (PO4), a monosaccharide, and a nitrogenous base. 2. There are four nitrogenous bases used by DNA: Adenine (A), Thymine (T), Guanine (G), and Cytosine ( C ). ...
... Lipids- Non-polar, hydrophobic Proteins Nucleic Acids- DNA & RNA 1. Made of monomers called nucleotides; The nucleotides are made of phosphate (PO4), a monosaccharide, and a nitrogenous base. 2. There are four nitrogenous bases used by DNA: Adenine (A), Thymine (T), Guanine (G), and Cytosine ( C ). ...
Browning reaction
... black discoloration is noticeable when the shrimp is injured. Whole shrimp that continue to live after being injured present a different behaviour: those in stage C are bound to recover from traumatism through cicatrization, in the first place, while those in stage D, perhaps because of forthcoming ...
... black discoloration is noticeable when the shrimp is injured. Whole shrimp that continue to live after being injured present a different behaviour: those in stage C are bound to recover from traumatism through cicatrization, in the first place, while those in stage D, perhaps because of forthcoming ...
Microbiology - Chapter 7 & 8
... (blood, eggs, etc) Some organisms are almost impossible to culture because of their strict parasitic-fastidious nature (syphilis, leprosy) ...
... (blood, eggs, etc) Some organisms are almost impossible to culture because of their strict parasitic-fastidious nature (syphilis, leprosy) ...
Biology
... Double sugar molecules are called Disaccharides. Disaccharides include sucrose or table sugar. It is made of a glucose and fructose bonded together. ...
... Double sugar molecules are called Disaccharides. Disaccharides include sucrose or table sugar. It is made of a glucose and fructose bonded together. ...
Consortium for Educational Communication
... The term ‘enzyme’ was introduced by Kuhne in 1878. It is taken from the Greek word Zume (Zyme) meaning ‘Leaven’. Enzymes are the principles found in Leaven. The first observation was carried out on vegetable and animal cell free extracts. Berzilius in 1836 coined the term catalysis which in Greek me ...
... The term ‘enzyme’ was introduced by Kuhne in 1878. It is taken from the Greek word Zume (Zyme) meaning ‘Leaven’. Enzymes are the principles found in Leaven. The first observation was carried out on vegetable and animal cell free extracts. Berzilius in 1836 coined the term catalysis which in Greek me ...
Enzymes - دانشکده پزشکی
... Phosphorylation is the most common type of modification Two important classes of enzymes are: – Kinases Add a phosphate group to another protein/enzyme (phosphorylation) ...
... Phosphorylation is the most common type of modification Two important classes of enzymes are: – Kinases Add a phosphate group to another protein/enzyme (phosphorylation) ...
HUMANIZATION N-GLYCOSYLATION PATHWAY PLANTS PLANT
... genes (e.g. approximately 30.000 in humans). These genes are stored in the nucleus of every cell in the form of DNA, a long polymer built up by monomers called nucleotides. Each nucleotide consists of a C-5-sugar (2`-deoxyribose) and a phosphate by which it is linked to the next nucleotide via a pho ...
... genes (e.g. approximately 30.000 in humans). These genes are stored in the nucleus of every cell in the form of DNA, a long polymer built up by monomers called nucleotides. Each nucleotide consists of a C-5-sugar (2`-deoxyribose) and a phosphate by which it is linked to the next nucleotide via a pho ...
Ultramer® Oligonucleotides - Integrated DNA Technologies
... Standard desalted Ultramer Oligonucleotides are perfect for site-directed mutagenesis, for use as templates for in vitro transcription, and in a variety of other molecular biology applications that require high purity oligonucleotides of length 45–200 nt. For applications in which higher purity is r ...
... Standard desalted Ultramer Oligonucleotides are perfect for site-directed mutagenesis, for use as templates for in vitro transcription, and in a variety of other molecular biology applications that require high purity oligonucleotides of length 45–200 nt. For applications in which higher purity is r ...
Chapter 06 Lecture PowerPoint - McGraw Hill Higher Education
... • Comparison of different s gene sequences reveals 4 regions of similarity among a wide variety of sources • Subregions 2.4 and 4.2 are involved in promoter -10 box and -35 box recognition • The s-factor by itself cannot bind to DNA, but DNA interaction with core unmasks a DNAbinding region of s • R ...
... • Comparison of different s gene sequences reveals 4 regions of similarity among a wide variety of sources • Subregions 2.4 and 4.2 are involved in promoter -10 box and -35 box recognition • The s-factor by itself cannot bind to DNA, but DNA interaction with core unmasks a DNAbinding region of s • R ...
Classification of Cell Membrane Proteins
... an associated hydrophobic affinity, which is often measured using a hydrophobic index. The Eisenberg hydrophobic index, which was used to analyze membrane-associated helices [20], is applied in this feature set. This index is normalized and ranges between -2.53 for R (the least hydrophobic) and 1.38 ...
... an associated hydrophobic affinity, which is often measured using a hydrophobic index. The Eisenberg hydrophobic index, which was used to analyze membrane-associated helices [20], is applied in this feature set. This index is normalized and ranges between -2.53 for R (the least hydrophobic) and 1.38 ...
RNA analysis on non-denaturing agarose gel electrophoresis
... band even on a non-denaturing gel. In other species the 28s rRNA is more robust, so it is still visible as a second band. Note: If your experimental RNA is shorter than expected and/or degraded according to electrophoresis data, prepare fresh RNA after checking the quality of RNA purification reage ...
... band even on a non-denaturing gel. In other species the 28s rRNA is more robust, so it is still visible as a second band. Note: If your experimental RNA is shorter than expected and/or degraded according to electrophoresis data, prepare fresh RNA after checking the quality of RNA purification reage ...
Chapter 3
... • Oxidoreductases – Catalyze oxidation-reduction reactions • Transferases – Transfer elements of one molecule to another • Hydrolases – Cleave bonds by adding water • Lyases – Groups of elements are removed to form a double bond or added to a double bond • Isomerases – Rearrangement of the structure ...
... • Oxidoreductases – Catalyze oxidation-reduction reactions • Transferases – Transfer elements of one molecule to another • Hydrolases – Cleave bonds by adding water • Lyases – Groups of elements are removed to form a double bond or added to a double bond • Isomerases – Rearrangement of the structure ...
Organic Chemistry/Fourth Edition: e-Text
... CH3(CH2)10CH2± groups of SDS associated with the lipophilic portions of the protein. The negatively charged sulfate groups are exposed to the water. The SDS molecules that they carry ensure that all the protein molecules are negatively charged and migrate toward the positive electrode. Furthermore, ...
... CH3(CH2)10CH2± groups of SDS associated with the lipophilic portions of the protein. The negatively charged sulfate groups are exposed to the water. The SDS molecules that they carry ensure that all the protein molecules are negatively charged and migrate toward the positive electrode. Furthermore, ...
What happened to my cousin Patrick O’Neill?
... bond in ATP is _____ and ____ energy to break the bond. A: Easy to break, releases B: Hard to break, requires C: Easy to break, requires D: Hard to break, releases ...
... bond in ATP is _____ and ____ energy to break the bond. A: Easy to break, releases B: Hard to break, requires C: Easy to break, requires D: Hard to break, releases ...
Central Dogma of Molecular Biology: How does the sequence of a
... nucleotide at the elongation site. Next, nucleophilic attack by the 3' hydroxyl of the first nucleotide on the α (inner) phosphorus of the second nucleotide generates the first phosphodiester bond and leaves an intact triphosphate at the 5' position of the first nucleotide. ...
... nucleotide at the elongation site. Next, nucleophilic attack by the 3' hydroxyl of the first nucleotide on the α (inner) phosphorus of the second nucleotide generates the first phosphodiester bond and leaves an intact triphosphate at the 5' position of the first nucleotide. ...
Introduction to Cellular and Molecular Biology (BIOL 190)
... Introduction to Cellular and Molecular Biology 2. Understand that catabolic pathways, like respiration and fermentation, release stored potential energy in organic compounds (i.e., food) to regenerate ATP from ADP + P. 3. Explain the principles of oxidation and reduction (i.e., redox) and know that ...
... Introduction to Cellular and Molecular Biology 2. Understand that catabolic pathways, like respiration and fermentation, release stored potential energy in organic compounds (i.e., food) to regenerate ATP from ADP + P. 3. Explain the principles of oxidation and reduction (i.e., redox) and know that ...
Biosynthesis
Biosynthesis (also called biogenesis or anabolism) is a multi-step, enzyme-catalyzed process where substrates are converted into more complex products in living organisms. In biosynthesis, simple compounds are modified, converted into other compounds, or joined together to form macromolecules. This process often consists of metabolic pathways. Some of these biosynthetic pathways are located within a single cellular organelle, while others involve enzymes that are located within multiple cellular organelles. Examples of these biosynthetic pathways include the production of lipid membrane components and nucleotides.The prerequisite elements for biosynthesis include: precursor compounds, chemical energy (e.g. ATP), and catalytic enzymes which may require coenzymes (e.g.NADH, NADPH). These elements create monomers, the building blocks for macromolecules. Some important biological macromolecules include: proteins, which are composed of amino acid monomers joined via peptide bonds, and DNA molecules, which are composed of nucleotides joined via phosphodiester bonds.