Structure of a Plasmodium yoelii gene
... contain stretches of very conserved amino acid sequences; the site of phosphorylation (James et al. 1987), the FITC (fluorescein isothiocyanate)-binding region (believed to be a part of ATP binding region) (Filoteo et al. 1987) and the FSBA (5'-p-fluorosulfonyl-benzoyladenosine; an analog of ATP)-bi ...
... contain stretches of very conserved amino acid sequences; the site of phosphorylation (James et al. 1987), the FITC (fluorescein isothiocyanate)-binding region (believed to be a part of ATP binding region) (Filoteo et al. 1987) and the FSBA (5'-p-fluorosulfonyl-benzoyladenosine; an analog of ATP)-bi ...
Molecular Characterization of NADH-Dependent
... NAD(P)H-GOGATare highly unstable and occur in Iow abundance. Root nodule NADH-GOGAT from lupine (Benny and Boland, 1977), common bean (Chen and Cullimore, 1988, 1989), and alfalfa (Anderson et al., 1989) has been isolated and characterized, and its activity has been found to increase markedly during ...
... NAD(P)H-GOGATare highly unstable and occur in Iow abundance. Root nodule NADH-GOGAT from lupine (Benny and Boland, 1977), common bean (Chen and Cullimore, 1988, 1989), and alfalfa (Anderson et al., 1989) has been isolated and characterized, and its activity has been found to increase markedly during ...
actiona actionation of FFFFFrrrrractiona
... results must be consistent. Even if it were possible to determine the isotope effect at each carbon position for every single step in a reaction sequence, and even if it were possible to recognize in advance every significant feature of the network, it would never be possible to be sure that studyin ...
... results must be consistent. Even if it were possible to determine the isotope effect at each carbon position for every single step in a reaction sequence, and even if it were possible to recognize in advance every significant feature of the network, it would never be possible to be sure that studyin ...
Energy Calculations for 4-Hydroxy-3
... which amino acid residues interact with the hetero compound. The wiring diagram is Figure 5 displayed below. The ligplot of the hetero compound, Figure 6, was also obtained from the PDBSum website. The ligplot is a 2D visualization of the amino acid residues in the protein which interact with the he ...
... which amino acid residues interact with the hetero compound. The wiring diagram is Figure 5 displayed below. The ligplot of the hetero compound, Figure 6, was also obtained from the PDBSum website. The ligplot is a 2D visualization of the amino acid residues in the protein which interact with the he ...
Energy and Muscle Contraction
... Therefore, during this time, cellular respiration will be going at capacity, limited only by oxygen restraints. Glycolysis, on the other hand, will proceed at an accelerated rate for the purpose of gaining extra ATP. Note that during this time, pyruvate will be fed into the mitochondria as fast as s ...
... Therefore, during this time, cellular respiration will be going at capacity, limited only by oxygen restraints. Glycolysis, on the other hand, will proceed at an accelerated rate for the purpose of gaining extra ATP. Note that during this time, pyruvate will be fed into the mitochondria as fast as s ...
Peer-reviewed Article PDF
... The citric acid or the Krebs cycle, [1] comprises a series of chemical reactions utilized by all aerobic organisms to generate its energy through the oxidation of acetate derived from carbohydrates, fats and proteins [2]. The final outcome of the cycle releases carbon dioxide and chemical energy in ...
... The citric acid or the Krebs cycle, [1] comprises a series of chemical reactions utilized by all aerobic organisms to generate its energy through the oxidation of acetate derived from carbohydrates, fats and proteins [2]. The final outcome of the cycle releases carbon dioxide and chemical energy in ...
AP Biology Exam
... 30. Which of the following enzymes is best described as an endonuclease? a. Restriction enzyme b. Catalytic enzyme c. Anabolic enzyme d. Reverse transcriptase e. None of the above 31. Which ribosomal subunit promotes the binding of amino acids? a. 40S b. 60S c. 70S d. 90S e. None of the above 32. Wh ...
... 30. Which of the following enzymes is best described as an endonuclease? a. Restriction enzyme b. Catalytic enzyme c. Anabolic enzyme d. Reverse transcriptase e. None of the above 31. Which ribosomal subunit promotes the binding of amino acids? a. 40S b. 60S c. 70S d. 90S e. None of the above 32. Wh ...
METABOLIC AND ENDOCRINE ADAPTATIONS TO HEAT STRESS
... can be reversed and heat flow is directed into the animal. In that case, evaporative heat loss is the last resort and an animal has to increase cutaneous evaporative heat loss (e.g. sweating) and respiratory evaporative heat loss (e.g. panting) to maintain homeothermy. In addition, vapor pressure di ...
... can be reversed and heat flow is directed into the animal. In that case, evaporative heat loss is the last resort and an animal has to increase cutaneous evaporative heat loss (e.g. sweating) and respiratory evaporative heat loss (e.g. panting) to maintain homeothermy. In addition, vapor pressure di ...
Amino Acid Composition in Fillets of Mirror Crossbreds Common
... Experimental studies evaluating common carp have recently been extended to include specific issues of food safety regarding the marketing of this most common freshwater fish (Ježek and Buchtová 2007). The factors considered include carp fillet shelf-life under various experimental packaging conditio ...
... Experimental studies evaluating common carp have recently been extended to include specific issues of food safety regarding the marketing of this most common freshwater fish (Ježek and Buchtová 2007). The factors considered include carp fillet shelf-life under various experimental packaging conditio ...
Full Text - IDOSI Publications
... species [39]. In the present study, palm pollen grains were analyzed for their contents of 17 of the most quantitatively important amino acids. Data obtained are given in Table 4 showed that palm pollen grains contained 8 essential amino acids and 9 nonessential amino acids. The major essential amin ...
... species [39]. In the present study, palm pollen grains were analyzed for their contents of 17 of the most quantitatively important amino acids. Data obtained are given in Table 4 showed that palm pollen grains contained 8 essential amino acids and 9 nonessential amino acids. The major essential amin ...
Molecular characterization of two Bangladeshi infectious bursal
... second ORF encodes a 110-kDa polyprotein, which is autocatalytically cleaved [12,26] to produce viral proteins VP2 (40 kDa), VP4 (28 kDa), and VP3 (32 kDa). Using sequence analysis and reverse genetics, VP2 from segment A [34,36], VP1 from segment B [14], or both [2,3] have been found to contribute ...
... second ORF encodes a 110-kDa polyprotein, which is autocatalytically cleaved [12,26] to produce viral proteins VP2 (40 kDa), VP4 (28 kDa), and VP3 (32 kDa). Using sequence analysis and reverse genetics, VP2 from segment A [34,36], VP1 from segment B [14], or both [2,3] have been found to contribute ...
The Utilization by Yeasts of Acids of the Tricarboxylic Acid Cycle
... The contents of the Warburg flask side arm were mixed with those of the main compartment, and a t the same moment 175 pmole of sodium acetate in 0.5 ml. water were added to the pipette flask and the contents we1 mixed. When the rate of acetate oxidation was steady (usually after about 20 min.) the a ...
... The contents of the Warburg flask side arm were mixed with those of the main compartment, and a t the same moment 175 pmole of sodium acetate in 0.5 ml. water were added to the pipette flask and the contents we1 mixed. When the rate of acetate oxidation was steady (usually after about 20 min.) the a ...
T. TRIOSE PHOSPHATE ISOMERASE Background
... passing through a transition state in which both C1 and C2 have substantial sp2 character. The second mechanism is a proton transfer mechanism, in which deprotonation of GAP leads to the formation of an enediolate intermediate which rearranges to form DHAP upon reprotonation (Figure T.4B). The stron ...
... passing through a transition state in which both C1 and C2 have substantial sp2 character. The second mechanism is a proton transfer mechanism, in which deprotonation of GAP leads to the formation of an enediolate intermediate which rearranges to form DHAP upon reprotonation (Figure T.4B). The stron ...
Ocimum sanctum Induced hepatic damage R.Bhuvaneswari Dr.K.Jegatheesan
... Toxicity of chemicals majorly affects all kinds of plants and animals. Excess of any kind of compounds will be harmful to life [1].Liver plays a major role in detoxification and is generally the major site for intense metabolism[2].It is also a site of biotransformation, of toxic compounds were conv ...
... Toxicity of chemicals majorly affects all kinds of plants and animals. Excess of any kind of compounds will be harmful to life [1].Liver plays a major role in detoxification and is generally the major site for intense metabolism[2].It is also a site of biotransformation, of toxic compounds were conv ...
ISOAMYLASE FROM PSEUDOMONAS AMYLODERAMOSA
... The P. amyloderamosa isoamylase is an extracellular enzyme that is secreted by the production strain to the fermentation broth. The fermentation broth is subsequently purified and concentrated to obtain isoamylase activity within a desired range. Isoamylase is then formulated with glucose and maltos ...
... The P. amyloderamosa isoamylase is an extracellular enzyme that is secreted by the production strain to the fermentation broth. The fermentation broth is subsequently purified and concentrated to obtain isoamylase activity within a desired range. Isoamylase is then formulated with glucose and maltos ...
Chapter X-1: The Plant Cell and the Cell Cycle
... air “restored” by vegetation could support the breathing of animals. air is “restored” only in the presence of light and only by the green parts of the plant. photosynthesis has a light-dependent stage and a light-independent stage. isolated chloroplasts are able to produce O2 in the absence of ligh ...
... air “restored” by vegetation could support the breathing of animals. air is “restored” only in the presence of light and only by the green parts of the plant. photosynthesis has a light-dependent stage and a light-independent stage. isolated chloroplasts are able to produce O2 in the absence of ligh ...
Putrescine oxidase of Micrococcus rubens : primary
... pH 7.5) containing 0.5 YONonidet P-40, 1 x Denhardt's solution and 50 pg salmon sperm DNA ml-' was carried out at 60 "C for 2 h. Hybridization was then done with the 32P-labelledprobes at 42 "C for 16 h in the same buffer. The nitrocellulose filters were washed three times in 2 x SSC (1 x SSC is 0.1 ...
... pH 7.5) containing 0.5 YONonidet P-40, 1 x Denhardt's solution and 50 pg salmon sperm DNA ml-' was carried out at 60 "C for 2 h. Hybridization was then done with the 32P-labelledprobes at 42 "C for 16 h in the same buffer. The nitrocellulose filters were washed three times in 2 x SSC (1 x SSC is 0.1 ...
Amino Acid Requirements and Post-absorptive Metabolism in Cattle
... directly addresses the general concern regarding the environmental footprint of animal production. Excretion of N, especially urinary, may become a potential source of water and air pollution, the latter as N2O, a green-house gas, or as small particulate aerosols having a negative effect on air qual ...
... directly addresses the general concern regarding the environmental footprint of animal production. Excretion of N, especially urinary, may become a potential source of water and air pollution, the latter as N2O, a green-house gas, or as small particulate aerosols having a negative effect on air qual ...
handout
... Eukaryotes have 80S ribosomes. 40S small subunit 60S large subunit Ribosomes in the mitochondria are similar to those in prokaryotes. 40S (small) subunit finds AUG (start codon) near the 5’ end, and translation begins when the 60S large subunits binds. ...
... Eukaryotes have 80S ribosomes. 40S small subunit 60S large subunit Ribosomes in the mitochondria are similar to those in prokaryotes. 40S (small) subunit finds AUG (start codon) near the 5’ end, and translation begins when the 60S large subunits binds. ...
Isolation and Characterization of Two Polypeptides
... epithelium, they may not be the only ones. Several minor polypeptides with molecular weights of 48,000, 51,000, and 53,000 daltons are also found in the urea extracts. When filaments are formed from urea extracts of esophagus, these minor polypeptides are quantitatively recovered through several cyc ...
... epithelium, they may not be the only ones. Several minor polypeptides with molecular weights of 48,000, 51,000, and 53,000 daltons are also found in the urea extracts. When filaments are formed from urea extracts of esophagus, these minor polypeptides are quantitatively recovered through several cyc ...
The Biochemistry of C 4 Photosynthesis
... During the evolution of C 4 photosynthesis from C~ plants, the MC developed a high level of carbonic anhydrase (CA) and PEP carboxylase for initial CO2 fixation in the cytoplasm, and pyruvate, orthophosphate (Pi) dikinase in the chloroplasts for provision of PEP, the HCOg acceptor. It is equally imp ...
... During the evolution of C 4 photosynthesis from C~ plants, the MC developed a high level of carbonic anhydrase (CA) and PEP carboxylase for initial CO2 fixation in the cytoplasm, and pyruvate, orthophosphate (Pi) dikinase in the chloroplasts for provision of PEP, the HCOg acceptor. It is equally imp ...
Metabolism
Metabolism (from Greek: μεταβολή metabolē, ""change"") is the set of life-sustaining chemical transformations within the cells of living organisms. These enzyme-catalyzed reactions allow organisms to grow and reproduce, maintain their structures, and respond to their environments. The word metabolism can also refer to all chemical reactions that occur in living organisms, including digestion and the transport of substances into and between different cells, in which case the set of reactions within the cells is called intermediary metabolism or intermediate metabolism.Metabolism is usually divided into two categories: catabolism, the breaking down of organic matter by way of cellular respiration, and anabolism, the building up of components of cells such as proteins and nucleic acids. Usually, breaking down releases energy and building up consumes energy.The chemical reactions of metabolism are organized into metabolic pathways, in which one chemical is transformed through a series of steps into another chemical, by a sequence of enzymes. Enzymes are crucial to metabolism because they allow organisms to drive desirable reactions that require energy that will not occur by themselves, by coupling them to spontaneous reactions that release energy. Enzymes act as catalysts that allow the reactions to proceed more rapidly. Enzymes also allow the regulation of metabolic pathways in response to changes in the cell's environment or to signals from other cells.The metabolic system of a particular organism determines which substances it will find nutritious and which poisonous. For example, some prokaryotes use hydrogen sulfide as a nutrient, yet this gas is poisonous to animals. The speed of metabolism, the metabolic rate, influences how much food an organism will require, and also affects how it is able to obtain that food.A striking feature of metabolism is the similarity of the basic metabolic pathways and components between even vastly different species. For example, the set of carboxylic acids that are best known as the intermediates in the citric acid cycle are present in all known organisms, being found in species as diverse as the unicellular bacterium Escherichia coli and huge multicellular organisms like elephants. These striking similarities in metabolic pathways are likely due to their early appearance in evolutionary history, and their retention because of their efficacy.