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How Does Biotechnology Affect Individuals, Society, and the
How Does Biotechnology Affect Individuals, Society, and the

... makeup of that organism. The process of gene transfer is used in many fields, including medicine and agriculture. • Gene manipulation ...
Foundations in Microbiology
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Genetics PowerPoint
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High school - The American Society of Human Genetics
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Parent organism - Office of the Gene Technology Regulator
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Fundamentals of Biotechnology
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... In DNA cloning, a DNA fragment that contains a gene of interest is inserted into a cloning vector or plasmid. The same restriction enzyme (here, EcoRI) is used to cut both (A) the plasmid carrying a gene for antibiotic resistance and (B) a DNA strand containing the gene of interest. The plasmid is o ...
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CHAPTER 6: RECOMBINANT DNA TECHNOLOGY
CHAPTER 6: RECOMBINANT DNA TECHNOLOGY

... TRANSFORMATION : The first step in transformation is to select a piece of DNA to be inserted into a vector. The second step is to cut that piece of DNA with a restriction enzyme and then ligase the DNA insert into the vector with DNA Ligase. The insert contains a selectable marker which allows for i ...
biocp_mar20
biocp_mar20

... H.B.4C Sex cells are formed by a process of cell division in which the number of chromosomes per cell is halved after replication. With the exception of sex chromosomes, for each chromosome in the body cells of a multicellular organism, there is a second similar, but not identical, chromosome. Altho ...
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Genetic engineering



Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.
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