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Supplementary Figure Legends (doc 52K)
Supplementary Figure Legends (doc 52K)

... these hydrocarbons in incubations with the corresponding unlabelled substrates as measured by HPLC (naphthalene and phenanthrene) or GCMS (n-hexadecane) (squares). (a) phenanthrene; (b) naphthalene; (c) n-hexadecane. The endpoint for these SIP incubations was determined to be 5 days. Each data point ...
Biology 164 Laboratory Genetic Mutants of the Prodigiosin
Biology 164 Laboratory Genetic Mutants of the Prodigiosin

... Prodigiosin Synthesis in Serratia marcescens In this laboratory exercise you will use the explanatory system to study the genetic control of a biochemical pathway that produces an end product called prodigiosin. This is a deep red pigment found within the bacterium Serratia marcescens and causes th ...
Engineering of steroid biotransformation in rhodococcus van
Engineering of steroid biotransformation in rhodococcus van

... chosen in a comparative study of several Rhodococcus strains as a suitable (i.e. genetically accessible and sterol degrading) parent strain for this study (Chapter 2). An electrotransformation protocol allowed transformation efficiencies of up to 1·106 transformants per µg of plasmid DNA (pMVS301). ...
pdf
pdf

... The presence of a copper-containing dissimilatory nitrite reductase gene (nirK) was discovered in several isolates of ␤-subdivision ammonia-oxidizing bacteria using PCR and DNA sequencing. PCR primers Cunir3 and Cunir4 were designed based on published nirK sequences from denitrifying bacteria and us ...
Chapter 23 PowerPoint 2016 - Spring
Chapter 23 PowerPoint 2016 - Spring

... • Natural selection increases the frequencies of alleles that enhance survival and reproduction • Adaptive evolution occurs as the match between an organism and its environment increases – Because the environment can change, adaptive evolution is a continuous process • Genetic drift and gene flow do ...
No Evidence of Neandertal mtDNA Contribution to Early Modern
No Evidence of Neandertal mtDNA Contribution to Early Modern

... has demonstrated that these individuals carried closely related mtDNAs that are not found among current humans. However, these results do not definitively resolve the question of a possible Neandertal contribution to the gene pool of modern humans since such a contribution might have been erased by ...
Tutorial: RNA-Seq Analysis Part II (Tracks): Non-Specific
Tutorial: RNA-Seq Analysis Part II (Tracks): Non-Specific

... Figure 10: The dot in the scatter plot corresponding to the gene "Rps13" and the corresponding region in the trackm list "zoomed-to-selection". area showing the visible reads by grabbing at the bottom of the track's editor and dragging it downwards. You will get a view similar to that in figure 11. ...
Characteristics and Traits
Characteristics and Traits

... same is true for many other plants and for virtually all animals. Diploid organisms utilize meiosis to produce haploid gametes, which contain one copy of each homologous chromosome that unite at fertilization to create a diploid zygote. For cases in which a single gene controls a single characterist ...
special - Microbiology
special - Microbiology

... not proven, to include nutrient limitation, the substrate mycelium gives rise to aerial hyphae, which yield chains of uninucleoidal spores to provide a mechanism for dispersal and the colonization of new environments (Chater & Losick, 1996). Streptomycetes also exhibit a remarkable capacity for bioc ...
Spinocerebellar Ataxia with Oculomotor Apraxia
Spinocerebellar Ataxia with Oculomotor Apraxia

... elevated serum alpha-fetoprotein, while our patient presented normal values. Genetic examination on chromosome 9q34 showed SETX mutation: three nucleotidic variations in heterozygous state were present: c.2975A > G(K992R), c.4365T > C(S1459P) and c.6341A > G(E2114G). From the data available in the l ...
Benchmarking two commonly used Saccharomyces
Benchmarking two commonly used Saccharomyces

... Received 3 February 2015 Received in revised form 29 July 2015 Accepted 3 September 2015 Available online 11 September 2015 ...
Application of rpoB sequence similarity analysis, REP‐PCR and
Application of rpoB sequence similarity analysis, REP‐PCR and

... candidate as a genome similarity predictor, as this gene encodes for the b subunit of RNA polymerase and has been proposed as an alternative to 16S rRNA for phylogenetic and biodiversity studies (Drancourt and Raoult 2002; Da Mota et al. 2004). This gene is common to all bacteria, exists as a single ...
Full Text  - Genes | Genomes | Genetics
Full Text - Genes | Genomes | Genetics

... database currently lists ~350 potential canine models for human diseases. In roughly 200 of these, the causative genetic defect is known (Nicholas and Hobbs 2014; http://omia.angis.org.au/home/). Different muscular dystrophies also have been described in dogs. Golden Retrievers with a Duchenne-like ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA

... of cells and nucleic acids prior to DNA purification. DDH was carried out as described by De Ley et al. (1970) under consideration of the modifications described by Huss et al. (1983) using a model Cary 100 Bio UV/VIS-spectrophotometer with a Peltier-thermostat-equipped 666 multicell changer and a t ...
Leukaemia Section t(1;14)(q21;q32) MUC1/IGH Atlas of Genetics and Cytogenetics in Oncology and Haematology
Leukaemia Section t(1;14)(q21;q32) MUC1/IGH Atlas of Genetics and Cytogenetics in Oncology and Haematology

... Genes involved and proteins ...
Document
Document

... and turn them into virus factories in the______________________cycle. 20. In the lytic cycle, a virus joins a cell and injects it with the virus’s______________________material. 21. During the lytic cycle, a virus’s genes take over the host and make______________________ 22. Newly made viruses break ...
Blue cone monochromacy: Causative mutations and associated
Blue cone monochromacy: Causative mutations and associated

... analyzed by agarose gel electrophoresis. All amplified products were then purified enzymatically. In dH2O, 1 µl ExoSAP-IT (GE Healthcare Ltd, Buckinghamshire, UK) was added to an aliquot of PCR product (1–6 µl containing approximately 500 ng) to create a volume of 26 µl. The samples were incubated a ...
Selection in backcross programmes
Selection in backcross programmes

... results start with the integration of the Bt transgene into different maize genetic backgrounds (Ragot et al. 1995). In this case, there is a single target, which is a well known transgenic construction, so the ‘marker’ equals the target without recombination. This confirmed the theoretical predicti ...
Enlis Genome Research - Release Notes
Enlis Genome Research - Release Notes

... - Main Application: Added Homozygous Regions Detector tool. Find regions of the genome with "runs" of consecutive homozygous variants. For rare disease analysis, these regions may indicate a consanguineous union, and provide a starting point for finding recessive disease. In tumor samples, these reg ...
REVIEW ARTICLE Gene cassettes
REVIEW ARTICLE Gene cassettes

... account for a significant proportion of the antibioticresistance genes found in Gram-negative bacteria of the Enterobacteriaceae family and the genus Psezldomonas. For most antibiotics, several quite distinct resistance genes are found, and in some cases the mechanism by which resistance is conferre ...
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA
Sequence analysis of 16S rRNA, gyrB and catA genes and DNA

... of cells and nucleic acids prior to DNA purification. DDH was carried out as described by De Ley et al. (1970) under consideration of the modifications described by Huss et al. (1983) using a model Cary 100 Bio UV/VIS-spectrophotometer with a Peltier-thermostat-equipped 666 multicell changer and a t ...
2001.Genetica.Carrol.. - University of Kentucky
2001.Genetica.Carrol.. - University of Kentucky

... goldenrain tree, Koelreuteria elegans, which since about 1955 has been increasingly planted for landscaping purposes (for details see Carroll & Boyd, 1992). Precisely how soon after its introduction this ...
NCEA Level 1 Science (90948) 2014 Assessment Schedule
NCEA Level 1 Science (90948) 2014 Assessment Schedule

... If a dominant allele was present, then individual 5 would be a tongue roller. So therefore they must have only both recessive alleles present. Individual 6 is a tongue roller, and so must have at least one dominant allele present for tongue rolling to be expressed. Both of 6’s children are a nontong ...
Genetic risk factors for chronic obstructive pulmonary disease
Genetic risk factors for chronic obstructive pulmonary disease

... cigarette smoking varies. Ideally, one would need multigeneration families, in which there were similar levels of exposure to cigarette smoke. However, this is extremely unlikely because of age- and gender-related differences in the prevalence of smoking. In addition, most patients with COPD do not ...
California
California

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Genetic engineering



Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.
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