Systematic and Applied Microbiology
... performed in the USA and better crop yields were recorded (E. Triplett, personal communication). Maize Klebsiella pneumoniae isolates had less virulence determinants than clinical isolates [11]. The search for genetic virulence determinants has been approached by comparing bacteria within the same s ...
... performed in the USA and better crop yields were recorded (E. Triplett, personal communication). Maize Klebsiella pneumoniae isolates had less virulence determinants than clinical isolates [11]. The search for genetic virulence determinants has been approached by comparing bacteria within the same s ...
Identification of sixteen single-nucleotide polymorphism markers in
... was extracted from adductor muscle using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek, Norcross, USA). Primers were first examined for PCR in eight wild individuals. PCR was performed in 20 µL reaction volume containing 12.3 µL H2 O miliQ, 2 µL PCR buffer (Mg2+ Plus) 10×, 1.6 µL dNTP 2.5 mM, 0.8 µL of ...
... was extracted from adductor muscle using the E.Z.N.A Mollusc DNA Kit (Omega Bio-Tek, Norcross, USA). Primers were first examined for PCR in eight wild individuals. PCR was performed in 20 µL reaction volume containing 12.3 µL H2 O miliQ, 2 µL PCR buffer (Mg2+ Plus) 10×, 1.6 µL dNTP 2.5 mM, 0.8 µL of ...
Evaluation of genomic DNA from paraffin
... separated by a clear space with oedematous fluid and in some cases there is also a diffuse infiltration of subendocardial fibrosis (Tidholm & Jönsson 2005). In a study, 64 of 65 (98%) dogs with confirmed DCM were positive for attenuated wavy fibers. In 147 dogs with other heart disease than DCM only ...
... separated by a clear space with oedematous fluid and in some cases there is also a diffuse infiltration of subendocardial fibrosis (Tidholm & Jönsson 2005). In a study, 64 of 65 (98%) dogs with confirmed DCM were positive for attenuated wavy fibers. In 147 dogs with other heart disease than DCM only ...
Involvement of respiratory chain in biofilm formation in - HAL
... gram-negative strict-anaerobe bacterium and constitutes one of the main pathogen responsible for periodontal diseases (2). Periodontal diseases are chronic inlammatory infections induced by bacteria organised in bioilm. More than 500 bacteria species take part in this bioilm including P. gingivalis ...
... gram-negative strict-anaerobe bacterium and constitutes one of the main pathogen responsible for periodontal diseases (2). Periodontal diseases are chronic inlammatory infections induced by bacteria organised in bioilm. More than 500 bacteria species take part in this bioilm including P. gingivalis ...
Nucleic Acids Research
... proteins of these 2 organisms still exhibit considerable amino acid homology. In fact, when the yeast iso-i and chicken cytochrome c proteins are aligned properly, 5 distinct regions of extensive amino acid homology are observed (Fig. 1). If maximum homology were conserved at the nucleic acid level, ...
... proteins of these 2 organisms still exhibit considerable amino acid homology. In fact, when the yeast iso-i and chicken cytochrome c proteins are aligned properly, 5 distinct regions of extensive amino acid homology are observed (Fig. 1). If maximum homology were conserved at the nucleic acid level, ...
Chapter. 13(Meiosis & Sexual Life Cycles)
... A Comparison of Mitosis and Meiosis • Mitosis conserves the number of chromosome sets, producing cells that are genetically identical to the parent cell. • Meiosis reduces the number of chromosomes sets from two (diploid) to one (haploid), producing cells with variety genetically different from eac ...
... A Comparison of Mitosis and Meiosis • Mitosis conserves the number of chromosome sets, producing cells that are genetically identical to the parent cell. • Meiosis reduces the number of chromosomes sets from two (diploid) to one (haploid), producing cells with variety genetically different from eac ...
Deletion of the gene encoding the reductase component of 3
... resulting in the formation of 4-cholesten-3-one. However, recent studies on some Mycobacterium strains suggested that 3-hydroxysteroid dehydrogenase, rather than cholesterol oxidase, is responsible for the first step oxidation [5,6]. The degradation continues with side chain cleavage, taking place v ...
... resulting in the formation of 4-cholesten-3-one. However, recent studies on some Mycobacterium strains suggested that 3-hydroxysteroid dehydrogenase, rather than cholesterol oxidase, is responsible for the first step oxidation [5,6]. The degradation continues with side chain cleavage, taking place v ...
Note for Guidance on the Quality, Preclinical and Clinical
... nucleic acids which has been added or modified for the purposes of gene transfer should be thoroughly described, including origin, identification, physico-chemical as well as functional characterisation, and expected function in the final product. ...
... nucleic acids which has been added or modified for the purposes of gene transfer should be thoroughly described, including origin, identification, physico-chemical as well as functional characterisation, and expected function in the final product. ...
Genetics: Mendelian Genetics Patterns of Inheritance
... earlobes, others have free earlobes. The genes that influence these traits are inherited independently. As a result, some people who can roll their tongues also have attached earlobes, while other tongue rollers have detached earlobes. Independent assortment was first described in the midnineteenth ...
... earlobes, others have free earlobes. The genes that influence these traits are inherited independently. As a result, some people who can roll their tongues also have attached earlobes, while other tongue rollers have detached earlobes. Independent assortment was first described in the midnineteenth ...
H - Cloudfront.net
... Mendel needed to answer one more question: When alleles are being segregated during gamete formation, does the segregation of one pair alleles have any affect on the segregation of a different pair of alleles? In other words, does the gene that determines if a pea plant is tall or dwarf have any af ...
... Mendel needed to answer one more question: When alleles are being segregated during gamete formation, does the segregation of one pair alleles have any affect on the segregation of a different pair of alleles? In other words, does the gene that determines if a pea plant is tall or dwarf have any af ...
Biology WarmUp: Meiosis Vocabulary Review 1. What does it
... 6. In fruit flies, the gene for star eye and speck wing are on the same chromosome, yet offspring from star-eyed, speckwinged parents often inherit star eyes without the speck wings. How is this possible? Make a diagram to illustrate the process that is responsible for the recombination. ...
... 6. In fruit flies, the gene for star eye and speck wing are on the same chromosome, yet offspring from star-eyed, speckwinged parents often inherit star eyes without the speck wings. How is this possible? Make a diagram to illustrate the process that is responsible for the recombination. ...
Phospholipid synthesis in Borrelia burgdorferi: BB0249 and BB0721
... alignment, the predicted protein for BB0721 has slight homology to Pgs of Bacillus subtilis, Treponema pallidum, Mycoplasma genitalium and Mycoplasma pneumoniae (16 % similarity and 8 % identity). However, BB0721 possesses a conserved CDP-alcohol phosphatidyltransferase motif, DG(X)2AR(X)8G(X)3D(X)3 ...
... alignment, the predicted protein for BB0721 has slight homology to Pgs of Bacillus subtilis, Treponema pallidum, Mycoplasma genitalium and Mycoplasma pneumoniae (16 % similarity and 8 % identity). However, BB0721 possesses a conserved CDP-alcohol phosphatidyltransferase motif, DG(X)2AR(X)8G(X)3D(X)3 ...
Chapter 13 Meiosis
... Inheritance of Genes • Genes are the units of heredity, and are made up of segments of DNA • Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) • Each gene has a specific location called a locus on a certain chromosome • Most DNA is packaged into chromoso ...
... Inheritance of Genes • Genes are the units of heredity, and are made up of segments of DNA • Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) • Each gene has a specific location called a locus on a certain chromosome • Most DNA is packaged into chromoso ...
Ref - SQA
... Sources and use of cellulases, arabanase, pectinases and amylases to overcome these problems.” New content statement added “Genetic modification of flavr savr tomato”. Notes amended to read “Gene for polygalacturonase (a pectinase which softens fruit) is cloned to produce RNA complementary to mRNA f ...
... Sources and use of cellulases, arabanase, pectinases and amylases to overcome these problems.” New content statement added “Genetic modification of flavr savr tomato”. Notes amended to read “Gene for polygalacturonase (a pectinase which softens fruit) is cloned to produce RNA complementary to mRNA f ...
The Novel Gene HOMOLOGOUS PAIRING
... by somatic culture and regeneration (Hirochika et al., 1996; Yamazaki et al., 2001). In the ND0016 of 600 sterile lines, complete male- and female-sterile plants segregated but with normal plant morphology (Figure 1A). The sterile phenotype segregated as a single recessive mutation (fertile:sterile ...
... by somatic culture and regeneration (Hirochika et al., 1996; Yamazaki et al., 2001). In the ND0016 of 600 sterile lines, complete male- and female-sterile plants segregated but with normal plant morphology (Figure 1A). The sterile phenotype segregated as a single recessive mutation (fertile:sterile ...
GeneMorph II EZClone Domain Mutagenesis Kit
... Random mutagenesis is a powerful tool for elucidating protein structurefunction relationships and for modifying proteins to improve or alter their characteristics. Error-prone PCR is a random mutagenesis technique for generating amino acid substitutions in proteins, domains or promoter elements by i ...
... Random mutagenesis is a powerful tool for elucidating protein structurefunction relationships and for modifying proteins to improve or alter their characteristics. Error-prone PCR is a random mutagenesis technique for generating amino acid substitutions in proteins, domains or promoter elements by i ...
9th Grade Reading Problems of the Day
... Still, she said, the thought of dark green tomatoes “kind of bugged me.” Why weren’t the leaves dark green, too? About a year ago, she and her colleagues, including Dr. Giovannoni, decided to investigate. The weed genes, they found, replaced a disabled gene in a tomato’s fruit but not in its leaves. ...
... Still, she said, the thought of dark green tomatoes “kind of bugged me.” Why weren’t the leaves dark green, too? About a year ago, she and her colleagues, including Dr. Giovannoni, decided to investigate. The weed genes, they found, replaced a disabled gene in a tomato’s fruit but not in its leaves. ...
Dopa- responsive dystonia
... reduced penetrance. This means that only about 30 – 40% of people who carry the gene will actually go on to develop DRD. ...
... reduced penetrance. This means that only about 30 – 40% of people who carry the gene will actually go on to develop DRD. ...
Restriction Enzyme Digest and Plasmid mapping
... On the other hand, separation of very small (less than 1 kb) can be better achieved in higher percentage agarose gel, such as a 1.5-2% gel. Making DNA Visible DNA is colorless so DNA fragments in the gel cannot be seen during electrophoresis. The loading dye (or loading buffer) does not stain the DN ...
... On the other hand, separation of very small (less than 1 kb) can be better achieved in higher percentage agarose gel, such as a 1.5-2% gel. Making DNA Visible DNA is colorless so DNA fragments in the gel cannot be seen during electrophoresis. The loading dye (or loading buffer) does not stain the DN ...
Running Head: PERSONALITY AND WELL-BEING
... Given that subjective well-being variance is mostly stable and not the result of environmental or demographic factors prompted some to suggest that individuals have biologically-based, stable well-being “set points” (Lykken & Tellegen, 1996). This hypothesis is supported by behavior genetic studies ...
... Given that subjective well-being variance is mostly stable and not the result of environmental or demographic factors prompted some to suggest that individuals have biologically-based, stable well-being “set points” (Lykken & Tellegen, 1996). This hypothesis is supported by behavior genetic studies ...
Chpt9_Transposition.doc
... occupy approximately half the human genome and a substantially greater fraction of some plant genomes! These movable elements are ubiquitous in the biosphere, and are highly successful in propagating themselves. We now realize that some transposable elements are also viruses, for instance, some retr ...
... occupy approximately half the human genome and a substantially greater fraction of some plant genomes! These movable elements are ubiquitous in the biosphere, and are highly successful in propagating themselves. We now realize that some transposable elements are also viruses, for instance, some retr ...
Special Report on Genetics and: Gout 23 Selected Articles From 199
... Cerebrovascular Disease -- Disorders which result in defective brain arteries, including stroke (both hemorrhagic and ischemic). Abstract 2006 Genetically determined gout: characteristics of the course. [Article in Russian] ...
... Cerebrovascular Disease -- Disorders which result in defective brain arteries, including stroke (both hemorrhagic and ischemic). Abstract 2006 Genetically determined gout: characteristics of the course. [Article in Russian] ...
Genetic engineering
Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.