Real time PCR based determination of gene copy numbers in
... integration is possible, complicating the molecular analysis. To overcome these drawbacks and also in order to deal with increasing numbers of expression strains from high throughput experiments, an additional reliable and faster method for copy number determination of integrated expression cassette ...
... integration is possible, complicating the molecular analysis. To overcome these drawbacks and also in order to deal with increasing numbers of expression strains from high throughput experiments, an additional reliable and faster method for copy number determination of integrated expression cassette ...
Wheat biotechnology: A minireview
... on the basis of gfp expression alone. Amongst the other scorable markers, luciferase gene from the fire fly, Photinus pyranus, has been successfully used in stable transformation of wheat (Lonsdale et al. 1998; Harvey et al. 1999). Luciferase and modified versions of gfp permit nondestructive analys ...
... on the basis of gfp expression alone. Amongst the other scorable markers, luciferase gene from the fire fly, Photinus pyranus, has been successfully used in stable transformation of wheat (Lonsdale et al. 1998; Harvey et al. 1999). Luciferase and modified versions of gfp permit nondestructive analys ...
Identification of Potential Corynebacterium ammoniagenes Purine
... (GMP), have been used as flavor-enhancing food additives. Corynebacterium ammoniagenes (C. ammoniagenes) is a Gram-positive coryneform bacterium used in the industrial production of metabolic compounds, including IMP and GMP purine nucleotides [7, 13]. The C. ammoniagenes strains used for the produc ...
... (GMP), have been used as flavor-enhancing food additives. Corynebacterium ammoniagenes (C. ammoniagenes) is a Gram-positive coryneform bacterium used in the industrial production of metabolic compounds, including IMP and GMP purine nucleotides [7, 13]. The C. ammoniagenes strains used for the produc ...
VCR 221 - Potato - UC Davis Plant Sciences
... Despres, C., M. Sabaelleil, S.R. Rivard and D. Morse. 1994. Molecular cloning of two Solanum chacoense S-alleles and a hypothesis concerning their evolution. Sexual Plant Reproduction 7:169-176. du Jardin, P. 1990. Homologies to plastid DNA in the nuclear and mitochondrial genomes of potato. TAG 79: ...
... Despres, C., M. Sabaelleil, S.R. Rivard and D. Morse. 1994. Molecular cloning of two Solanum chacoense S-alleles and a hypothesis concerning their evolution. Sexual Plant Reproduction 7:169-176. du Jardin, P. 1990. Homologies to plastid DNA in the nuclear and mitochondrial genomes of potato. TAG 79: ...
Obligate phototrophy in cyanobacteria: more than a lack of sugar
... through utilization of their plant-like photosynthetic process. They nevertheless possess a respiratory activity (for a review see [1]) and all the enzymes necessary for sugar catabolism [2,3]. Only approximately half of the strains tested, however, are capable of using this pathway to perform heter ...
... through utilization of their plant-like photosynthetic process. They nevertheless possess a respiratory activity (for a review see [1]) and all the enzymes necessary for sugar catabolism [2,3]. Only approximately half of the strains tested, however, are capable of using this pathway to perform heter ...
Using CRISPR for genetic alteration_Joffrey Maine
... and complex F0 should be treated as mosaic Phenotype data should be acquired from F1 onwards, once the alleles have been segregated and fully characterised ...
... and complex F0 should be treated as mosaic Phenotype data should be acquired from F1 onwards, once the alleles have been segregated and fully characterised ...
Observing in real time the evolution of artemisinin Open Access
... among parasites in the Mekong basin, a foundation on which K13 mutants not only occur, but survive, and often rise to high prevalence under selection by artemisinins. This work from the Greater Mekong region makes it clear that the evolution of resistance to artemisinin is still evolving rapidly. Mo ...
... among parasites in the Mekong basin, a foundation on which K13 mutants not only occur, but survive, and often rise to high prevalence under selection by artemisinins. This work from the Greater Mekong region makes it clear that the evolution of resistance to artemisinin is still evolving rapidly. Mo ...
Dragon Genetics
... Preparations of Popsicle Stick Chromosomes Each popsicle stick represents a pair of homologous chromosomes, with the alleles of one of the homologous chromosomes on one side of the popsicle stick and the alleles of the other homologous chromosome on the other side of the popsicle stick. The appropri ...
... Preparations of Popsicle Stick Chromosomes Each popsicle stick represents a pair of homologous chromosomes, with the alleles of one of the homologous chromosomes on one side of the popsicle stick and the alleles of the other homologous chromosome on the other side of the popsicle stick. The appropri ...
The Isolation of Mutagen-Sensitive nuv Mutants of
... More than 200 mutants Aspergillus of nidulans were isolated as hypersensitiveto the monofunctional alkylating agent MNNG and/or UV-irradiation (designated nuu mutants). Of these, 23 were selected for further characterization. All were markedly hypersensitive to both MNNG and the quasi-UVmimetic muta ...
... More than 200 mutants Aspergillus of nidulans were isolated as hypersensitiveto the monofunctional alkylating agent MNNG and/or UV-irradiation (designated nuu mutants). Of these, 23 were selected for further characterization. All were markedly hypersensitive to both MNNG and the quasi-UVmimetic muta ...
CTY Genetics Syllabus
... • Lecture/Class Notes: Methods of gene regulation- promoters, silencers, and operons (35 minutes) • HexBot Battles of the lac operon (30 minutes) Robot simulation of effect of inducers/repressors • Scientific Drawing: Tracing genes from DNA-RNA-Protein Structure with ID of regulator locations in e ...
... • Lecture/Class Notes: Methods of gene regulation- promoters, silencers, and operons (35 minutes) • HexBot Battles of the lac operon (30 minutes) Robot simulation of effect of inducers/repressors • Scientific Drawing: Tracing genes from DNA-RNA-Protein Structure with ID of regulator locations in e ...
Käfer, E. and D. Luk
... therefore, likely to be defective in DNA repair rather than uptake or metabolism of MMS. Most of the mutants listed in Tables 1 and 2 have been induced by 4NQ0 (4-nitroquinoline-l-oxide) as described for Aspergillus by Bal et al. (1977 Mutat. Res. 56:153156). More recently, 3 further cases, mus (131 ...
... therefore, likely to be defective in DNA repair rather than uptake or metabolism of MMS. Most of the mutants listed in Tables 1 and 2 have been induced by 4NQ0 (4-nitroquinoline-l-oxide) as described for Aspergillus by Bal et al. (1977 Mutat. Res. 56:153156). More recently, 3 further cases, mus (131 ...
Hd6, a rice quantitative trait locus involved in photoperiod
... Many efforts have been made to map such genes, known as quantitative trait loci (QTLs), because of their biological and agronomic importance. The development of a high-density linkage map based on DNA markers made it possible to map QTLs with high resolution (1). However, it has been difficult to id ...
... Many efforts have been made to map such genes, known as quantitative trait loci (QTLs), because of their biological and agronomic importance. The development of a high-density linkage map based on DNA markers made it possible to map QTLs with high resolution (1). However, it has been difficult to id ...
Methylation of the Factor IX Gene is the Main Source of Mutations
... employed. Amino acids of the factor IX protein were enumerated as suggested in [5]. In the promoter region of the human factor IX gene, 21 mutations were registered; approximately 730 mutations were found in coding regions, and 55 mutations were present in splicing sites. Among the registered mutati ...
... employed. Amino acids of the factor IX protein were enumerated as suggested in [5]. In the promoter region of the human factor IX gene, 21 mutations were registered; approximately 730 mutations were found in coding regions, and 55 mutations were present in splicing sites. Among the registered mutati ...
A Guide to Conditions of the Retina
... treatments and, ultimately, cures. Some conditions, such as certain types of age-related macular degeneration and diabetesrelated sight loss, are already being successfully treated in Ireland. For other rare, genetic conditions, we are still in the early investigative stages, but positively moving f ...
... treatments and, ultimately, cures. Some conditions, such as certain types of age-related macular degeneration and diabetesrelated sight loss, are already being successfully treated in Ireland. For other rare, genetic conditions, we are still in the early investigative stages, but positively moving f ...
Interval mapping of QTLs controlling yield
... number, pod number, 1000-seed weight, 1000-yield, and seed protein content. Characterization of each QTL included identification of QTL position with reference to the flanking markers, estimation of the part of variance explained by this QTL, and determination of its gene action. The yield-related t ...
... number, pod number, 1000-seed weight, 1000-yield, and seed protein content. Characterization of each QTL included identification of QTL position with reference to the flanking markers, estimation of the part of variance explained by this QTL, and determination of its gene action. The yield-related t ...
Genetic Algorithms and Related Optimization Techniques: An
... a solution. This fitness (or evaluation) function needs to be very efficient, as it may need to be called thousands even millions - of times. But you do not need... ...the final solution to be optimal. ...speed (this varies) RMI Workshop - October 7, 1999 ...
... a solution. This fitness (or evaluation) function needs to be very efficient, as it may need to be called thousands even millions - of times. But you do not need... ...the final solution to be optimal. ...speed (this varies) RMI Workshop - October 7, 1999 ...
Platelet antigens and antibody detection
... NAITP, often known as feto-maternal alloimmune thrombocytopenia (FMAIT), occurs as a consequence of maternal immunization against fetal platelet alloantigens inherited from the father. Maternal IgG alloantibodies then cross the placenta and cause immune destruction of platelets in utero, a situation ...
... NAITP, often known as feto-maternal alloimmune thrombocytopenia (FMAIT), occurs as a consequence of maternal immunization against fetal platelet alloantigens inherited from the father. Maternal IgG alloantibodies then cross the placenta and cause immune destruction of platelets in utero, a situation ...
Gene Section HFE (hemochromatosis) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... is associated with increased risk to childhood acute lympho-blastic leukemia in boys only. This association has not been noted in other studies in Finland, Spain and Mexico. In Italy, adult leukemia shows an association with H63D mutation. ...
... is associated with increased risk to childhood acute lympho-blastic leukemia in boys only. This association has not been noted in other studies in Finland, Spain and Mexico. In Italy, adult leukemia shows an association with H63D mutation. ...
meiosis - juan
... Inheritance of Genes • Genes are the units of heredity, and are made up of segments of DNA • Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) • Each gene has a specific location called a locus on a certain chromosome • Most DNA is packaged into chromoso ...
... Inheritance of Genes • Genes are the units of heredity, and are made up of segments of DNA • Genes are passed to the next generation via reproductive cells called gametes (sperm and eggs) • Each gene has a specific location called a locus on a certain chromosome • Most DNA is packaged into chromoso ...
network - bioinf leipzig
... Regulatory interactions can also be inferred directly from data = reverse engineering of biological pathways/networks from data. In the example above time-series expression data61is used to infer a directed and signed graph based on delayed correlations. ...
... Regulatory interactions can also be inferred directly from data = reverse engineering of biological pathways/networks from data. In the example above time-series expression data61is used to infer a directed and signed graph based on delayed correlations. ...
aabb
... the surface of red blood cells. • These markers are called: A substance or B substance. • They are recognized by antibodies present in the blood serum of individuals for foreign carbohydrate markers. • There are two types of RBC antibody: Anti-A & Anti-B • The genes for creating these antibodies are ...
... the surface of red blood cells. • These markers are called: A substance or B substance. • They are recognized by antibodies present in the blood serum of individuals for foreign carbohydrate markers. • There are two types of RBC antibody: Anti-A & Anti-B • The genes for creating these antibodies are ...
The Evolutionary History of Human and Chimpanzee Y
... (Pan troglodytes) Y-chromosome sequences revealed that although there are no lineage-specific gene-disruptive mutations in the X-degenerate portion of the Y-chromosome fixed within humans, surprisingly, 4 genes, CYorf15B, TBL1Y, TMSB4Y, and USP9Y, are disrupted by one or more splice site or prematur ...
... (Pan troglodytes) Y-chromosome sequences revealed that although there are no lineage-specific gene-disruptive mutations in the X-degenerate portion of the Y-chromosome fixed within humans, surprisingly, 4 genes, CYorf15B, TBL1Y, TMSB4Y, and USP9Y, are disrupted by one or more splice site or prematur ...
Volume 46 - Tomato Genetics Cooperative
... containing breeding line Massachusetts #2, for this gene was found in 'an aberrant plant of the breeding line Massachusetts #2'. This plant was resistant to race 4, but susceptible to another race, which later appeared to be 2.3.4.11 (Kerr & Patrick, 1977). The Cf-11 line was crossed to other (unkn ...
... containing breeding line Massachusetts #2, for this gene was found in 'an aberrant plant of the breeding line Massachusetts #2'. This plant was resistant to race 4, but susceptible to another race, which later appeared to be 2.3.4.11 (Kerr & Patrick, 1977). The Cf-11 line was crossed to other (unkn ...
Microarray-based comparative genomic hybridisation (array CGH)
... array CGH. This is because balanced chromosome rearrangements do not result in any loss or gain of chromosome material. It will also not detect some types of polyploidy (more than the usual 2 sets of chromosomes), such as triploidy (three sets of chromosomes). A standard karyotype is still available ...
... array CGH. This is because balanced chromosome rearrangements do not result in any loss or gain of chromosome material. It will also not detect some types of polyploidy (more than the usual 2 sets of chromosomes), such as triploidy (three sets of chromosomes). A standard karyotype is still available ...
Genetic engineering
Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.