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24 Recombination Hotspots in Nonallelic Homologous Recombination
24 Recombination Hotspots in Nonallelic Homologous Recombination

... to these developments, sufficient numbers of breakpoints of selected NAHR rearrangements have been characterized at the DNA sequence level to resolve the distribution of crossovers in these cases. This has similarly led to the identification of NAHR hotspots within paralogous sequences. This chapter ...
Quantitative trait loci affecting amylose, amylopectin and starch
Quantitative trait loci affecting amylose, amylopectin and starch

... and locate QTLs (quantitative trait loci) on a genetic map consisting mainly of RFLP markers for genes with known function. The genetic material was recombinant inbred lines originating from parents differing in starch structure (dent vs. flint). Kernels were harvested from field grown plants for tw ...
SVPaper050711
SVPaper050711

Variation and Selection at the CAULIFLOWER Floral Homeotic
Variation and Selection at the CAULIFLOWER Floral Homeotic

... the linker I-region; the former is believed to contain the transcriptional activation domain of MADS-box proteins (Riechmann and Meyerowitz 1997). Molecular analyses reveal a large amount of variation at the BoCAL locus (Figure 2 and Table 1). A total of 87 variant sites are present in these sampled ...
Practice Genetic Problems PMB 220 1. In working with a haploid
Practice Genetic Problems PMB 220 1. In working with a haploid

... 1. In working with a haploid organism, you cross a purple strain (ad-) strain of mating type (A) and a white (ad+) strain of mating type (B). If ad- and ad+ are alleles of one gene and the A and B alleles are an independently inherited gene on a separate chromosome pair, what progeny do you expect t ...
1 FRQ-interacting RNA Helicase (FRH) Mediates Negative and
1 FRQ-interacting RNA Helicase (FRH) Mediates Negative and

... those of WC-1, consistent both with loss of positive feedback (FRQ-dependent WC-1 stabilization) and with a reduced level of the FRQ-mediated WCC phosphorylation that leads to high WCC activity accompanied by rapid transcription-associated turnover. FRH overexpression promotes WC-1 accumulation, con ...
The Work of Gregor Mendel
The Work of Gregor Mendel

... because peas are small and easy to grow. A single pea plant can produce hundreds of offspring. Today we call peas a “model system.” ...
Section Summaries With IPC Review • Concise two
Section Summaries With IPC Review • Concise two

... chapter content and developing study skills • Builds a record of students’ work to use as a study aid for quizzes and tests ...
Advances in Environmental Biology  Escherichia coli from Frozen Bovine Meat in Algeria
Advances in Environmental Biology Escherichia coli from Frozen Bovine Meat in Algeria

... Virulence factors include hemolysins and Shiga toxins (Stx) [13,33]. Members of family include Stx1 and Stx2 toxins encoded by stx1 and stx2 genes, respectively. Shiga toxins are the major virulence factors of EHEC which have cytotoxicity effects for human and animal eukaryotic cells and cause dange ...
Recruitment of CRABS CLAW to promote nectary development
Recruitment of CRABS CLAW to promote nectary development

... perianth positions in basal taxa to central positions associated with reproductive organs in more derived taxa. In addition, extrafloral nectaries are currently known in 68 angiosperm families (Elias, 1983). Their structures and locations are also diverse across the families, although they occur mos ...
gsea user guide
gsea user guide

... Expression data. GSEA works with expression data from any source. Although different processing methods produce different types of expression values (for example, natural scale versus logged expression levels for Affymetrix data, or Affymetrix data versus two-color ratio data), any type of expressio ...
Different susceptibility of two animal species infected with isogenic
Different susceptibility of two animal species infected with isogenic

... strains within a species, have different pathogenicities for different animal hosts. For example, strains of M. tuberculosis are not as virulent as strains of M. bovis in rabbits but both species appear similarly virulent in guinea pigs (Dannenberg & Collins, 2001); and South Indian strains of M. tu ...
Designs for QTL detection in livestock and their implications for MAS
Designs for QTL detection in livestock and their implications for MAS

... insemination (AI) and field data recording is widespread, such as in dairy cattle. In these halfsib designs, genotypes are collected on a number of grandsires and their half-sib offspring. Phenotypes are either collected on the half-sib offspring themselves or on a group of progeny from each half-si ...
Preview Sample 3
Preview Sample 3

... MUTATION, DNA REPAIR, AND CANCER ...
UNIT I - cloudfront.net
UNIT I - cloudfront.net

... transformation ...
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES
Sun J, Ke J, Johnson JL, Nikolau BJ, Wurtele ES

... To confirm directly that C A C 2 cDNA codes for the biotin carboxylase subunit of the heteromeric, plastid-localized ACCase, and to characterize the biochemical properties of the CAC2 protein, we expressed it in E. coli (Fig. 2). An expressed GST-CAC2 fusion protein was used to generate antiserum (F ...
Inbreeding in Swine, nsif-fs4 - National Swine Improvement Federation
Inbreeding in Swine, nsif-fs4 - National Swine Improvement Federation

... have done well. Certain families may demonstrate superior performance, but if a family group has poor average performance, it should be discarded quickly. Inbreeding also promotes an increase in prepotency which is the ability of an individual to consistently pass on its characteristics. This prepot ...
Improvement of Aspergillus nidulans penicillin production by
Improvement of Aspergillus nidulans penicillin production by

... 1999). Many bacterial infections, which in previous times often caused the death of patients and even resulted in several worldwide epidemics, suddenly could be cured. However, from the first description of the inhibition of bacteria through Penicillium notatum cultures to the first human trials or ev ...
LATHYROIDES, encoding a WUSCHEL
LATHYROIDES, encoding a WUSCHEL

... During organ development, many key regulators have been identified in plant genomes, which play conserved role among plant species to control the organ identities and/or determine the organ size and shape. It is intriguing that whether these key regulators can acquire diverse function and be integra ...
Quantitative trait locus mapping in natural populations
Quantitative trait locus mapping in natural populations

... population from two parental species, races or populations. In theory, such an analysis could be performed in an unmanipulated population in a hybrid zone (if one exists), but understanding the genetic basis of species differences is nearly always more tractable in specially created mapping crosses. ...
Identification of Plant Genes for Abiotic Stress Resistance
Identification of Plant Genes for Abiotic Stress Resistance

... signals. The application of ABA to plants mimics the effect of a stress condition. As many abiotic stresses ultimately result in desiccation of the cell and osmotic imbalance, there is an overlap in the expression pattern of stress genes after cold, drought, high salt or ABA application. This sugges ...
How to recognize and diagnose chromosome rearrangements. David D. Perkins Background
How to recognize and diagnose chromosome rearrangements. David D. Perkins Background

... has been used to identify most of the Neurospora rearrangements that have been detected since 1950. Viable ascospores are black, but ascospores that contain a recombination-generated deficiency are inviable and fail to darken. (These are referred to as 'hyaline' or 'white'.) Presence of a heterozygo ...
redundancy and regulatory interactions between two
redundancy and regulatory interactions between two

... FAR cDNA clones showed that four utilized the alternative splice site. The variation occurs within a region of the MADS-box protein known as the I-domain (Ma et al., 1991), the length and sequence composition of which have been postulated to affect the heterodimerization specificity amongst MADS-box ...
Warren, ST and Ashley, CT: Triplet repeat expansion mutations: The example of fragile X syndrome. Annual Review of Neuroscience 18:77-99 (1995).
Warren, ST and Ashley, CT: Triplet repeat expansion mutations: The example of fragile X syndrome. Annual Review of Neuroscience 18:77-99 (1995).

... the X chromosome near the distal tip (Lubs 1969). Sutherland (1977) later identified this constriction as an inducible fragile site at Xq27.3 (Figure 1); it is induced in tissue culture by perturbation of folic acid or thymidine pools, which results in limited amounts of dTIP for DNA synthesis (Suth ...
The virulence plasmids of Salmonella
The virulence plasmids of Salmonella

... and VII, and in 2 of 9 strains of subspecies IV [11]. The spv region of subspecies I harbors five genes, spvRABCD, organized as a regulon and well conserved among serovars (Fig. 1). Sequencing of spv genes has provided no clues about their function, except in the case of SpvR, a 33-kDa polypeptide h ...
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Genetic engineering



Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.
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