Saccharomycopsis fibuligera and Yarrowia lipol`ica
... As for the protoplast fusants, A6/A18 and A18/112, all the stable mitotic segregants obtained from them from mitotic sectoring had, in general, a thin, filmy white phenotype (Table 1). Colonies of the segregants on YPSS had aerial hyphae, were non-wrinkled and amylolytic and resembled S. fibdigera s ...
... As for the protoplast fusants, A6/A18 and A18/112, all the stable mitotic segregants obtained from them from mitotic sectoring had, in general, a thin, filmy white phenotype (Table 1). Colonies of the segregants on YPSS had aerial hyphae, were non-wrinkled and amylolytic and resembled S. fibdigera s ...
Nucleotide sequence and taxonomic value of the
... 1987), very little is known about the immunochemistry of this organism. One problem is that C. pneumoniae is difficult to isolate in tissue culture. Immunochemical and genetic studies should lead to simple and non-culturedependent diagnostic methodologies for this important organism, as has been the ...
... 1987), very little is known about the immunochemistry of this organism. One problem is that C. pneumoniae is difficult to isolate in tissue culture. Immunochemical and genetic studies should lead to simple and non-culturedependent diagnostic methodologies for this important organism, as has been the ...
Directions for Use Taq DNA Polymerase, 500U
... What is the source of AMRESCO’s UNG? The recombinant cod UNG is produced in an ung negative E. coli strain and is designed to replace the native UNG enzyme originating in Atlantic cod (Gadus morhua). What is the stability of recombinant cod UNG? UNG can tolerate multiple freeze-thaw cycles. It may a ...
... What is the source of AMRESCO’s UNG? The recombinant cod UNG is produced in an ung negative E. coli strain and is designed to replace the native UNG enzyme originating in Atlantic cod (Gadus morhua). What is the stability of recombinant cod UNG? UNG can tolerate multiple freeze-thaw cycles. It may a ...
Genetic mapping of mutations using phenotypic pools and
... polymorphisms with DNA markers as a function of recombination distance between the subject locus and the marker. In this section we describe the results of computer simulations to address this issue. Descrption of terns. The probability of encountering an alternate allele in the homozygous pool incr ...
... polymorphisms with DNA markers as a function of recombination distance between the subject locus and the marker. In this section we describe the results of computer simulations to address this issue. Descrption of terns. The probability of encountering an alternate allele in the homozygous pool incr ...
The Effect of OmpA Expression on Hfr Conjugation Efficiency
... strains. These results suggest that OmpA- strains, C156 and C158, are able to participate in Hfr conjugations; however, these conjugations are not as efficient as wild-type (C149) strains. Introduction of plasmid-expressed functional ompA did not improve the conjugation efficiencies of the respectiv ...
... strains. These results suggest that OmpA- strains, C156 and C158, are able to participate in Hfr conjugations; however, these conjugations are not as efficient as wild-type (C149) strains. Introduction of plasmid-expressed functional ompA did not improve the conjugation efficiencies of the respectiv ...
Genetic polymorphisms in diabetes: Influence on therapy with oral
... Mutations in the HNF1a gene Subjects with mutations in the HNF-1a gene usually develop diabetes in adolescence or early adulthood (27, 28). Mutations in HNF-1a result in progressive b-cell dysfunction with increasing treatment requirements and a higher risk of complications with age (29). Deteriorat ...
... Mutations in the HNF1a gene Subjects with mutations in the HNF-1a gene usually develop diabetes in adolescence or early adulthood (27, 28). Mutations in HNF-1a result in progressive b-cell dysfunction with increasing treatment requirements and a higher risk of complications with age (29). Deteriorat ...
here - Institute of Human Genetics
... The main treatment of CPT2 Deficiency is through a low fat diet. Children may be given a special milk formula called MCT (medium chain triglycerides). If the child is well or does not have any illness, he/she should eat regular meals and avoid fasting more than 6 hours. Carnitine, a natural substanc ...
... The main treatment of CPT2 Deficiency is through a low fat diet. Children may be given a special milk formula called MCT (medium chain triglycerides). If the child is well or does not have any illness, he/she should eat regular meals and avoid fasting more than 6 hours. Carnitine, a natural substanc ...
A Pseudomonas syringae pv. tomato DC3000 mutant
... non-host of Pto DC3000 and does not show symptoms when inoculated at a low level but responds with the HR at a high level (Mysore and Ryu, 2004). Understanding the factors that prevent DC3000 from being virulent in N. benthamiana and other non-host plants is important for at least three reasons. Fir ...
... non-host of Pto DC3000 and does not show symptoms when inoculated at a low level but responds with the HR at a high level (Mysore and Ryu, 2004). Understanding the factors that prevent DC3000 from being virulent in N. benthamiana and other non-host plants is important for at least three reasons. Fir ...
- University of Bath Opus
... sex-biased gene (Ellegren and Parsch, 2007). Evidence from both the worm (Cutter and Ward, 2005) and Drosophila(Gnad and Parsch, 2006) genomes demonstrates an increased number of male-biased genes through duplication. DNA sequences with no coding function could also provide a source for sex-biased ...
... sex-biased gene (Ellegren and Parsch, 2007). Evidence from both the worm (Cutter and Ward, 2005) and Drosophila(Gnad and Parsch, 2006) genomes demonstrates an increased number of male-biased genes through duplication. DNA sequences with no coding function could also provide a source for sex-biased ...
Preliminary study on resynthesis of winter oilseed - IHAR
... Many intergeneric and interspecific hybridizations within seed crops fail due to the inability of pollen germination on another stigma or too short pollen tubes do not reach the germ or cracking of pollen tubes takes place. To overcome these barriers the technique of in vitro culture of ovary and em ...
... Many intergeneric and interspecific hybridizations within seed crops fail due to the inability of pollen germination on another stigma or too short pollen tubes do not reach the germ or cracking of pollen tubes takes place. To overcome these barriers the technique of in vitro culture of ovary and em ...
DNA cytosine methylation in plant development
... Cytosine bases of the nuclear genome in higher plants are often extensively methylated. Cytosine methylation has been implicated in the silencing of both transposable elements (TEs) and endogenous genes, and loss of methylation may have severe functional consequences. The recent methylation profilin ...
... Cytosine bases of the nuclear genome in higher plants are often extensively methylated. Cytosine methylation has been implicated in the silencing of both transposable elements (TEs) and endogenous genes, and loss of methylation may have severe functional consequences. The recent methylation profilin ...
The new cardiac genetic testing panels
... to enable informative genetic testing for HCM and for this to be provided as an NHS service in the UK. Even earlier, in the 1980s, the BHF began to fund Dr (now BHF Professor) Steve Humphries in his search to identify the genetic causes of familial hyperlipidemia (FH). Continuous BHF funding for Pro ...
... to enable informative genetic testing for HCM and for this to be provided as an NHS service in the UK. Even earlier, in the 1980s, the BHF began to fund Dr (now BHF Professor) Steve Humphries in his search to identify the genetic causes of familial hyperlipidemia (FH). Continuous BHF funding for Pro ...
Plant speciation through chromosome instability and ploidy change
... Multivalent: Physical linkage of three or more chromosomes at the meiotic diakinesis-metaphase I stages through establishment of genetic cross-overs or chiasmata. Neo-functionalization: The acquisition of novel function in one of a pair of genes resulting from a gene or a whole genome duplication ev ...
... Multivalent: Physical linkage of three or more chromosomes at the meiotic diakinesis-metaphase I stages through establishment of genetic cross-overs or chiasmata. Neo-functionalization: The acquisition of novel function in one of a pair of genes resulting from a gene or a whole genome duplication ev ...
Relationships between a new type IV secretion system and the icm
... functional similarities and sequence homologies. First, both systems were shown to conjugate RSF1010-related plasmids between bacteria, using the icm/dot or vir gene products respectively. Second, the virulence of both systems was shown to be inhibited by an active RSF1010 conjugation system. Third, ...
... functional similarities and sequence homologies. First, both systems were shown to conjugate RSF1010-related plasmids between bacteria, using the icm/dot or vir gene products respectively. Second, the virulence of both systems was shown to be inhibited by an active RSF1010 conjugation system. Third, ...
A1071 GM Canola MON88302 AppR SD1
... the common soil bacterium Agrobacterium sp. The CP4 EPSPS protein has previously been assessed by FSANZ in a range of crops including canola. In comparison with a previous GM canola line developed by the Applicant and assessed by FSANZ (FSANZ 2000), MON88302 has tolerance to higher rates of glyphosa ...
... the common soil bacterium Agrobacterium sp. The CP4 EPSPS protein has previously been assessed by FSANZ in a range of crops including canola. In comparison with a previous GM canola line developed by the Applicant and assessed by FSANZ (FSANZ 2000), MON88302 has tolerance to higher rates of glyphosa ...
First genomic insights into members of a candidate bacterial
... were performed for proteins with matches to UniRef90 and IMG using a bit score threshold of 300, and one-way BLASTP matches with a bit score of 60 (Castelle et al., 2013). For COGs, RPS-BLAST against COG PSSMs from the CDD database (Marchler-Bauer et al., 2013) was performed using an e-value cutoff ...
... were performed for proteins with matches to UniRef90 and IMG using a bit score threshold of 300, and one-way BLASTP matches with a bit score of 60 (Castelle et al., 2013). For COGs, RPS-BLAST against COG PSSMs from the CDD database (Marchler-Bauer et al., 2013) was performed using an e-value cutoff ...
mendelian inheritance - E-Learning/An
... out by Joseph Kölreuter from 1761 to 1766. In crosses between different strains of tobacco plants, he found that the offspring were usually intermediate in appearance between the two parents. This led Kölreuter to conclude that both parents make equal genetic contributions to their offspring. Furthe ...
... out by Joseph Kölreuter from 1761 to 1766. In crosses between different strains of tobacco plants, he found that the offspring were usually intermediate in appearance between the two parents. This led Kölreuter to conclude that both parents make equal genetic contributions to their offspring. Furthe ...
The Good, the Bad, the Ugly - Ask a Biologist
... © Arizona Science Center & Ask A Biologist | Funded by the National Center for Research Resources of the National Institutes for Health | Page 10 ...
... © Arizona Science Center & Ask A Biologist | Funded by the National Center for Research Resources of the National Institutes for Health | Page 10 ...
Prediction and investigation of novel proteins in DNA double
... DSBs are primarily repaired by two independent and highly conserved pathways: homologous recombination (HR) and non-homologous end joining (NHEJ). HR requires a homologous sequence to repair DNA breaks, whereas NHEJ repair is achieved through direct ligation of the broken ends of DNA. The process of ...
... DSBs are primarily repaired by two independent and highly conserved pathways: homologous recombination (HR) and non-homologous end joining (NHEJ). HR requires a homologous sequence to repair DNA breaks, whereas NHEJ repair is achieved through direct ligation of the broken ends of DNA. The process of ...
Elmo genetics WMarble
... look at the traits they have and don’t have. Terminology is gone over as the terms and questions about the terms come up while the students look/ go through the recording sheet and the handout. Once they go through what each trait looks like, determine if it is dominant or recessive, and record thei ...
... look at the traits they have and don’t have. Terminology is gone over as the terms and questions about the terms come up while the students look/ go through the recording sheet and the handout. Once they go through what each trait looks like, determine if it is dominant or recessive, and record thei ...
lecture - Berkeley MCB
... In their own words “Up until now, it had seemed reasonable to postulate that constitutive mutants synthesized an endogenous inducer which was absent in inducible cells. The results described here suggest an exactly opposite hypothesis. The facts can be explained by the supposition that the i gene d ...
... In their own words “Up until now, it had seemed reasonable to postulate that constitutive mutants synthesized an endogenous inducer which was absent in inducible cells. The results described here suggest an exactly opposite hypothesis. The facts can be explained by the supposition that the i gene d ...
labs.bio.unc.edu
... Does clustering reflect gene density? Gene density and recombination frequency vary along the genome QTL could be clustered because they map to regions with many genes/cM Suggested for wheat (Peng et al. 2003) Expected theoretically (Noor et al. 2001) ...
... Does clustering reflect gene density? Gene density and recombination frequency vary along the genome QTL could be clustered because they map to regions with many genes/cM Suggested for wheat (Peng et al. 2003) Expected theoretically (Noor et al. 2001) ...
Systematic and Applied Microbiology - digital
... Results and discussion These sequences of strains BIFI-56 and BIFI-58 showed a 100% identity to the same fragment of the16S rRNA sequence from the E. faecium type strain (ATCC 19434, DSM 20477).Therefore the 16S rRNA sequence revealed that both strains were E. faecium isolates. In order to know if ...
... Results and discussion These sequences of strains BIFI-56 and BIFI-58 showed a 100% identity to the same fragment of the16S rRNA sequence from the E. faecium type strain (ATCC 19434, DSM 20477).Therefore the 16S rRNA sequence revealed that both strains were E. faecium isolates. In order to know if ...
drosophila melanogaster.
... expected to be fixed in this interval in both the marked and unmarked third chromosomes. Only fixation in the unmarked chromosome, however, could be detected in this experiment. Fixation of lethal genes in fact occurred, before subdivision, in lines 8A and 48B and after subdivision, in 48A2, 8Blc an ...
... expected to be fixed in this interval in both the marked and unmarked third chromosomes. Only fixation in the unmarked chromosome, however, could be detected in this experiment. Fixation of lethal genes in fact occurred, before subdivision, in lines 8A and 48B and after subdivision, in 48A2, 8Blc an ...
Genetic engineering
Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.