
Hailey Spelman - Determining Cellular Fate: Pre- and Postnatal Methylation Effects on Gene Expression
... effect of simple measures on methylation patterns during prenatal development are clear in their ability to change phenotypic expression. Based on this idea, if scientists could target methylation of specific genes, they could directly and selectively choose genes to be turned off. At this point, no ...
... effect of simple measures on methylation patterns during prenatal development are clear in their ability to change phenotypic expression. Based on this idea, if scientists could target methylation of specific genes, they could directly and selectively choose genes to be turned off. At this point, no ...
Full-Text PDF
... 2. Choice of Suitable Models to Study Mitochondrial Diseases Because of the complexity of the human organism and the difficulties associated with direct experimentation, it is necessary to make studies and analyses in model organisms. As will be seen, there is no perfect model system. 2.1. Mammalian ...
... 2. Choice of Suitable Models to Study Mitochondrial Diseases Because of the complexity of the human organism and the difficulties associated with direct experimentation, it is necessary to make studies and analyses in model organisms. As will be seen, there is no perfect model system. 2.1. Mammalian ...
MENDEL`S PRINCIPLES
... their passage from one generation to the next. Gregor Johann Mendel discovered these rules of inheritance; we derive and expand upon his rules in this chapter (fig. 2.1). In 1900, three botanists, Carl Correns of Germany, Erich von Tschermak of Austria, and Hugo de Vries of Holland, defined the rules ...
... their passage from one generation to the next. Gregor Johann Mendel discovered these rules of inheritance; we derive and expand upon his rules in this chapter (fig. 2.1). In 1900, three botanists, Carl Correns of Germany, Erich von Tschermak of Austria, and Hugo de Vries of Holland, defined the rules ...
Vocabulary Definitions
... Punnett square a mathematical model that predicts the probability of possible genotypes and the phenotypes resulting from a genetic cross (SRB, IG) recessive a form of a gene that is expressed only when a dominant allele is not present (SRB, IG) reproduce to create new individual organisms of the sa ...
... Punnett square a mathematical model that predicts the probability of possible genotypes and the phenotypes resulting from a genetic cross (SRB, IG) recessive a form of a gene that is expressed only when a dominant allele is not present (SRB, IG) reproduce to create new individual organisms of the sa ...
Close - Journal of Integrated OMICS
... Enterococci were obtained from 96 (98%) of the 98 samples analysed. E. hirae was the most prevalent detected species (35 isolates), followed by E. faecalis (30 isolates), E. faecium (27 isolates) and E. durans (4 isolates). E. coli isolates were detected in 90 (92%) of the 98 Iberian lynx faecal sam ...
... Enterococci were obtained from 96 (98%) of the 98 samples analysed. E. hirae was the most prevalent detected species (35 isolates), followed by E. faecalis (30 isolates), E. faecium (27 isolates) and E. durans (4 isolates). E. coli isolates were detected in 90 (92%) of the 98 Iberian lynx faecal sam ...
A Genetic-Based Evaluation of the Principal Tissue Reservoir for
... laboratories uncovered 12 additional cases of group A streptococcal invasive disease. In addition, isolates derived from normally sterile tissue sites of 9 patients were never sent to the state laboratory; since these 21 bacterial isolates were not available for molecular analyses, those cases were ...
... laboratories uncovered 12 additional cases of group A streptococcal invasive disease. In addition, isolates derived from normally sterile tissue sites of 9 patients were never sent to the state laboratory; since these 21 bacterial isolates were not available for molecular analyses, those cases were ...
Genetic balancers
... Linking lethals to morphological markers is especially helpful for analysis, as it is much easier to score for the absence of a particular morphological phenotype than to score arrested progeny. Furthermore, linked markers facilitate two-factor mapping, and markers common to an entire set of lethals ...
... Linking lethals to morphological markers is especially helpful for analysis, as it is much easier to score for the absence of a particular morphological phenotype than to score arrested progeny. Furthermore, linked markers facilitate two-factor mapping, and markers common to an entire set of lethals ...
Bonus, a Drosophila TIF1 homologue, is a chromatin
... and bon21B, a null allele (Fig. 1B) (BECKSTEAD et al. 2001). We observed that all bon434 and bon241 homozygous males died as first instar larvae, while all mutant females survived at least to the second instar larval stage. In contrast, a strong loss of function allele, bon21B, causes lethality in b ...
... and bon21B, a null allele (Fig. 1B) (BECKSTEAD et al. 2001). We observed that all bon434 and bon241 homozygous males died as first instar larvae, while all mutant females survived at least to the second instar larval stage. In contrast, a strong loss of function allele, bon21B, causes lethality in b ...
High diversity of the `Spumella-like` flagellates: an investigation
... two, i.e. a small and a large forms, were isolated per sample to avoid multiple isolation of the same clone. All strains possess a spherical to ovate cell body. The strains tended to either attach to the substratum when well fed or to actively swim when starved. Swimming cells tended to spin in smal ...
... two, i.e. a small and a large forms, were isolated per sample to avoid multiple isolation of the same clone. All strains possess a spherical to ovate cell body. The strains tended to either attach to the substratum when well fed or to actively swim when starved. Swimming cells tended to spin in smal ...
Analysis of acid-induced asr gene promoter of Enterobacteriaceae
... Deletion analysis of asr promoter region upstream the proposed pho box. In order to identify potential cis-regulatory sites in the asr promoter, deletion analysis of the DNA region upstream the –40 position was performed. The consecutive promoter deletions (p∆70, p∆37, p∆20, p∆21, p∆10, p∆4, p∆1 in ...
... Deletion analysis of asr promoter region upstream the proposed pho box. In order to identify potential cis-regulatory sites in the asr promoter, deletion analysis of the DNA region upstream the –40 position was performed. The consecutive promoter deletions (p∆70, p∆37, p∆20, p∆21, p∆10, p∆4, p∆1 in ...
Roux`s Arch Dev Biol 193, 283
... vide a means of obtaining information about different aspects of embryonic development. The number of gene functions affected indicates how many components are specific to the process. The kinds of phenotypic change observed in mutant embryos reveal parameters of the developmental system. Insights i ...
... vide a means of obtaining information about different aspects of embryonic development. The number of gene functions affected indicates how many components are specific to the process. The kinds of phenotypic change observed in mutant embryos reveal parameters of the developmental system. Insights i ...
Amplification of 16S rRNA Genes from Frankia Strains in Root
... Root nodules. The sources of the root nodules used in this study are listed in Table 1. In all cases the nodules were frozen at 2258C after collection or receipt at the University of Waikato. DNA isolation. Extreme care was used to avoid exogenous DNA in all solutions and during isolation of Frankia ...
... Root nodules. The sources of the root nodules used in this study are listed in Table 1. In all cases the nodules were frozen at 2258C after collection or receipt at the University of Waikato. DNA isolation. Extreme care was used to avoid exogenous DNA in all solutions and during isolation of Frankia ...
Epigenetic Effects of Psychological Stressors in Humans
... Epigenetic mechanisms including DNA methylation and histone modifications are the link between environment and genome. These mechanisms control gene expression and determine the genetic outcome of an organism. The main well investigated epigenetic mechanisms are DNA methylation and histone modificat ...
... Epigenetic mechanisms including DNA methylation and histone modifications are the link between environment and genome. These mechanisms control gene expression and determine the genetic outcome of an organism. The main well investigated epigenetic mechanisms are DNA methylation and histone modificat ...
Chapter 14
... trait and the white flower color a recessive trait • Mendel observed the same pattern of inheritance in six other pea plant characters, each represented by two traits • What Mendel called a “heritable factor” is what we now call a gene Copyright © 2008 Pearson Education Inc., publishing as Pearson B ...
... trait and the white flower color a recessive trait • Mendel observed the same pattern of inheritance in six other pea plant characters, each represented by two traits • What Mendel called a “heritable factor” is what we now call a gene Copyright © 2008 Pearson Education Inc., publishing as Pearson B ...
De novo mutations in human genetic disease
... affect specific genes and nucleotides. Mutations causing severe genetic diseases are often highly disruptive to gene function and tend to affect important domains of developmental genes. An open question is whether these mutations occur mainly in the germline, during embryogenesis or somatically. A ...
... affect specific genes and nucleotides. Mutations causing severe genetic diseases are often highly disruptive to gene function and tend to affect important domains of developmental genes. An open question is whether these mutations occur mainly in the germline, during embryogenesis or somatically. A ...
video slide
... trait and the white flower color a recessive trait • Mendel observed the same pattern of inheritance in six other pea plant characters, each represented by two traits • What Mendel called a “heritable factor” is what we now call a gene Copyright © 2008 Pearson Education Inc., publishing as Pearson B ...
... trait and the white flower color a recessive trait • Mendel observed the same pattern of inheritance in six other pea plant characters, each represented by two traits • What Mendel called a “heritable factor” is what we now call a gene Copyright © 2008 Pearson Education Inc., publishing as Pearson B ...
Feline Genetics: a Combinatorial Approach
... minor effect which piles up with the others, yielding a more gradual combined result than a unique gene could account for. Sometimes we shall present two alternative mathematical models to explain the same effect. If the two models give the same effect in all cases, they are mathematically equivalen ...
... minor effect which piles up with the others, yielding a more gradual combined result than a unique gene could account for. Sometimes we shall present two alternative mathematical models to explain the same effect. If the two models give the same effect in all cases, they are mathematically equivalen ...
Hemoglobin A2: origin, evolution, and aftermath
... these variants is much greater than would be expected for random mutation. Therefore, Petes suggested that these two variants could represent gene conversion events. Another variant that was found subsequent to Petes’ hypothesis, Hb Parchman (622 ala + glu, 50 ser thr), could also be due to gene con ...
... these variants is much greater than would be expected for random mutation. Therefore, Petes suggested that these two variants could represent gene conversion events. Another variant that was found subsequent to Petes’ hypothesis, Hb Parchman (622 ala + glu, 50 ser thr), could also be due to gene con ...
Biology 30 June 1999 Grade 12 Diploma Exam
... For hundreds of years, Chinese folk doctors have known an intriguing but mysterious fact. Drinking herbal tea brewed with a type of club moss (Huperzia serrata) can improve a person’s memory. About ten years ago, researchers at the Shanghai Institute of Materia Medica isolated a compound from the te ...
... For hundreds of years, Chinese folk doctors have known an intriguing but mysterious fact. Drinking herbal tea brewed with a type of club moss (Huperzia serrata) can improve a person’s memory. About ten years ago, researchers at the Shanghai Institute of Materia Medica isolated a compound from the te ...
A newly discovered Anaerococcus strain responsible for axillary
... contribute to axillary odor. Analysis with a recently developed next-generation sequencing method (without culturing) has confirmed that the skin microbiota contains Propionibacterium as well as other anaerobic bacteria (Costello et al., 2009). These findings led us to focus on investigating the rel ...
... contribute to axillary odor. Analysis with a recently developed next-generation sequencing method (without culturing) has confirmed that the skin microbiota contains Propionibacterium as well as other anaerobic bacteria (Costello et al., 2009). These findings led us to focus on investigating the rel ...
Nixon Evidence
... Giardia lamblia and Entamoeba histolytica are amitochondriate, microaerophilic protists which use fermentation enzymes like those of bacteria to survive anaerobic conditions within the intestinal lumen. Genes encoding fermentation enzymes and related electron transport peptides (e.g., ferredoxins) i ...
... Giardia lamblia and Entamoeba histolytica are amitochondriate, microaerophilic protists which use fermentation enzymes like those of bacteria to survive anaerobic conditions within the intestinal lumen. Genes encoding fermentation enzymes and related electron transport peptides (e.g., ferredoxins) i ...
Diagnosis of primary ciliary dyskinesia: searching for a gold standard
... sinopulmonary disease and immotile sperm [8]. Using light microscopy to evaluate ciliary beat and transmission electron microscopy (TEM) to examine ciliary ultrastructure, other patients with chronic sinopulmonary disease were diagnosed with ‘‘immotile cilia syndrome’’ [8]. Approximately 50% of the ...
... sinopulmonary disease and immotile sperm [8]. Using light microscopy to evaluate ciliary beat and transmission electron microscopy (TEM) to examine ciliary ultrastructure, other patients with chronic sinopulmonary disease were diagnosed with ‘‘immotile cilia syndrome’’ [8]. Approximately 50% of the ...
Regulation of Bacterial Conjugation in Microaerobiosis by
... The resulting fragment was purified with the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). After digestion with BamHI and EcoRI the amplified fragment was cloned onto pGEX4T-1 (GE Healthcare, Little Chalfont, UK) to obtain a fusion protein containing glutathione-S-transferase (GST) a ...
... The resulting fragment was purified with the Wizard SV Gel and PCR Clean-Up System (Promega, Madison, WI). After digestion with BamHI and EcoRI the amplified fragment was cloned onto pGEX4T-1 (GE Healthcare, Little Chalfont, UK) to obtain a fusion protein containing glutathione-S-transferase (GST) a ...
Genetic engineering
Genetic engineering, also called genetic modification, is the direct manipulation of an organism's genome using biotechnology. It is therefore a set of technologies used to change the genetic makeup of cells, including the transfer of genes within and across species boundaries to produce improved or novel organisms. New DNA may be inserted in the host genome by first isolating and copying the genetic material of interest using molecular cloning methods to generate a DNA sequence, or by synthesizing the DNA, and then inserting this construct into the host organism. Genes may be removed, or ""knocked out"", using a nuclease. Gene targeting is a different technique that uses homologous recombination to change an endogenous gene, and can be used to delete a gene, remove exons, add a gene, or introduce point mutations.An organism that is generated through genetic engineering is considered to be a genetically modified organism (GMO). The first GMOs were bacteria generated in 1973 and GM mice in 1974. Insulin-producing bacteria were commercialized in 1982 and genetically modified food has been sold since 1994. Glofish, the first GMO designed as a pet, was first sold in the United States December in 2003.Genetic engineering techniques have been applied in numerous fields including research, agriculture, industrial biotechnology, and medicine. Enzymes used in laundry detergent and medicines such as insulin and human growth hormone are now manufactured in GM cells, experimental GM cell lines and GM animals such as mice or zebrafish are being used for research purposes, and genetically modified crops have been commercialized.