An emerging role for prdm family genes in dorsoventral patterning of
... Prdm3, Prdm5, Prdm9, Prdm13, Prdm14, and Prdm16 bind DNA directly in a sequence dependent manner via their zinc-finger domains ([9, 35–43] reviewed in [1, 2]). While many Prdm proteins have only been tested for DNA binding using in vitro systems, ChIP-seq experiments (chromatin immunoprecipitation u ...
... Prdm3, Prdm5, Prdm9, Prdm13, Prdm14, and Prdm16 bind DNA directly in a sequence dependent manner via their zinc-finger domains ([9, 35–43] reviewed in [1, 2]). While many Prdm proteins have only been tested for DNA binding using in vitro systems, ChIP-seq experiments (chromatin immunoprecipitation u ...
Nieuwkoop`s Center
... Depletion of Maternal mRNAs Injection of a-sense morpholino oligonucleotide causes degradation of complementary mRNA thereby depleting the oocyte/embryo of that message and the encoded protein. Some maternal proteins may still be present depending on the actual stability of the protein. Technique w ...
... Depletion of Maternal mRNAs Injection of a-sense morpholino oligonucleotide causes degradation of complementary mRNA thereby depleting the oocyte/embryo of that message and the encoded protein. Some maternal proteins may still be present depending on the actual stability of the protein. Technique w ...
PDF
... The transition between pluripotency and multi-lineage commitment during early embryogenesis must be closely regulated to ensure correct spatial and temporal patterning of the embryo. But what regulates this crucial transition? According to Kristen Kroll and co-workers, part of the answer to this que ...
... The transition between pluripotency and multi-lineage commitment during early embryogenesis must be closely regulated to ensure correct spatial and temporal patterning of the embryo. But what regulates this crucial transition? According to Kristen Kroll and co-workers, part of the answer to this que ...
PDF
... The transition between pluripotency and multi-lineage commitment during early embryogenesis must be closely regulated to ensure correct spatial and temporal patterning of the embryo. But what regulates this crucial transition? According to Kristen Kroll and co-workers, part of the answer to this que ...
... The transition between pluripotency and multi-lineage commitment during early embryogenesis must be closely regulated to ensure correct spatial and temporal patterning of the embryo. But what regulates this crucial transition? According to Kristen Kroll and co-workers, part of the answer to this que ...
PDF
... The transition between pluripotency and multi-lineage commitment during early embryogenesis must be closely regulated to ensure correct spatial and temporal patterning of the embryo. But what regulates this crucial transition? According to Kristen Kroll and co-workers, part of the answer to this que ...
... The transition between pluripotency and multi-lineage commitment during early embryogenesis must be closely regulated to ensure correct spatial and temporal patterning of the embryo. But what regulates this crucial transition? According to Kristen Kroll and co-workers, part of the answer to this que ...
NEURAL TUBE
... notochordal process which passes through a phase in which it forms a hollow rod which subsequently opens to both the amniotic and yolk sac cavities, followed by reversion to a solid rod. ...
... notochordal process which passes through a phase in which it forms a hollow rod which subsequently opens to both the amniotic and yolk sac cavities, followed by reversion to a solid rod. ...
... exposure to oxidant chemicals causes stage-specific effects and overlapping but distinct patterns of altered gene expression in zebrafish embryos. Developmental changes in glutathione redox dynamics and transcription factor expression provide possible mechanisms for the stage-specific sensitivity to ...
Gene Section PTCH2 (patched homolog 2 (Drosophila)) Atlas of Genetics and Cytogenetics
... evidence that PTCH2 hay have a role in tumor development comes from knock-out mouse model systems. Thus while Ptch2(-/-) mice have no obvious phenotype and do not develop tumors, if they are crossed with Ptch1 (+/-) heterozygotes, then the resulting Ptch2(-/-)Ptch1(+/-) mice developed at a higher in ...
... evidence that PTCH2 hay have a role in tumor development comes from knock-out mouse model systems. Thus while Ptch2(-/-) mice have no obvious phenotype and do not develop tumors, if they are crossed with Ptch1 (+/-) heterozygotes, then the resulting Ptch2(-/-)Ptch1(+/-) mice developed at a higher in ...
- Wiley Online Library
... the reduced size of the single Shh mutant embryos, but also all ventral cell types, while dorsal cell types are still missing as in the single opb mutant (Eggenschwiler et al., 2001). Taken together, Rab23 inhibits signaling of Shh intracellularly and establishes the basis for the development of dor ...
... the reduced size of the single Shh mutant embryos, but also all ventral cell types, while dorsal cell types are still missing as in the single opb mutant (Eggenschwiler et al., 2001). Taken together, Rab23 inhibits signaling of Shh intracellularly and establishes the basis for the development of dor ...
1 2 - VCOMcc
... Mesodermal Derivatives o 4 types Axial Located on the midline Forms notochord Induce formation of neural tube Differentiate into nucleus pulposus of IV disc Paraxial Forms somites initially 42-44 pairs but some degenerate leaving 36 functional pairs Somatic cells differentiate into ...
... Mesodermal Derivatives o 4 types Axial Located on the midline Forms notochord Induce formation of neural tube Differentiate into nucleus pulposus of IV disc Paraxial Forms somites initially 42-44 pairs but some degenerate leaving 36 functional pairs Somatic cells differentiate into ...
Molecular myogenic program - Georgia Institute of Technology
... mean no muscle – Or at least, no specific-subset-of-muscles ...
... mean no muscle – Or at least, no specific-subset-of-muscles ...
answers
... Here, the mRNA is translated, and Pipe protein is consequently secreted from the anterior end of the embryo. As a sulfotransferase, it will make sulfur group modifications to the ECM there, leading to cleavage of proSpz to produce active Spz* in the anterior ECM. Spz* will activate Toll signaling, l ...
... Here, the mRNA is translated, and Pipe protein is consequently secreted from the anterior end of the embryo. As a sulfotransferase, it will make sulfur group modifications to the ECM there, leading to cleavage of proSpz to produce active Spz* in the anterior ECM. Spz* will activate Toll signaling, l ...
Molecular Basis of Development and Molecular Embryology
... If the AER is removed but beads containing p FGF 8 are added, limb development can continue, near to normal, if enough FGF 8 is added. ...
... If the AER is removed but beads containing p FGF 8 are added, limb development can continue, near to normal, if enough FGF 8 is added. ...
Evidence that Shh cooperates with a retinoic acid inducible co
... are the parental cell line of COS cells which are able to efficiently process Shh protein (Bumcrot et al., 1995). As shown in the immunoblot in Fig. 3, CV-1 and P19 cells transfected with chicken Shh produce a polypeptide of approximately 45 ×103 Mr which could correspond to the full length Shh. In ...
... are the parental cell line of COS cells which are able to efficiently process Shh protein (Bumcrot et al., 1995). As shown in the immunoblot in Fig. 3, CV-1 and P19 cells transfected with chicken Shh produce a polypeptide of approximately 45 ×103 Mr which could correspond to the full length Shh. In ...
PDF
... cilia-associated compartments determine the accessibility of Gli proteins to PKA and thus the activity of the Shh pathway in vertebrates. ...
... cilia-associated compartments determine the accessibility of Gli proteins to PKA and thus the activity of the Shh pathway in vertebrates. ...
Division of Morphogenesis
... ascidian embryos. Ciona intestinalis Noto4 (Ci-Noto4) was isolated as a gene downstream of Ci-Bra. This gene encodes a 307 amino-acid protein with a C-terminal phosphotyrosine interaction domain (PTB/PID). Expression of Ci-Noto4 commences at the neural plate stage and is specific to notochord cells. ...
... ascidian embryos. Ciona intestinalis Noto4 (Ci-Noto4) was isolated as a gene downstream of Ci-Bra. This gene encodes a 307 amino-acid protein with a C-terminal phosphotyrosine interaction domain (PTB/PID). Expression of Ci-Noto4 commences at the neural plate stage and is specific to notochord cells. ...
ppt
... Another level of control: one TF (gene) can activate or repress other genes, depending on promoter context ...
... Another level of control: one TF (gene) can activate or repress other genes, depending on promoter context ...
Exporter la page en pdf
... The nine vertebrate PAX transcription factors (PAX1-PAX9) play essential roles during early development and organogenesis. Pax genes were identified in vertebrates using their homology with the Drosophila melanogaster paired gene DNA-binding domain. PAX1-9 functions are largely conserved throughout v ...
... The nine vertebrate PAX transcription factors (PAX1-PAX9) play essential roles during early development and organogenesis. Pax genes were identified in vertebrates using their homology with the Drosophila melanogaster paired gene DNA-binding domain. PAX1-9 functions are largely conserved throughout v ...
Hedgehog signal transduction: recent findings Kent
... megalin–ligand interactions [15]. What role megalin’s binding to Shh could play in signal transduction is not clear. It is unlikely that megalin is a Shh receptor independent of Patched (Ptc). Megalin could, however, function as a coreceptor for Shh, and so would join the heparan sulphate proteoglyc ...
... megalin–ligand interactions [15]. What role megalin’s binding to Shh could play in signal transduction is not clear. It is unlikely that megalin is a Shh receptor independent of Patched (Ptc). Megalin could, however, function as a coreceptor for Shh, and so would join the heparan sulphate proteoglyc ...
Hedgehog proteins: expression and function in the thymus
... vertebrates(2), from fish to mammals. They are key players in most of vertebrate developmental processes and so, implicated in several types of human congenital abnormalities, including holoprosoencephalia, and cancer(3-5). The most pleiotropic of vertebrate Hh molecules is Shh. It orchestrates dive ...
... vertebrates(2), from fish to mammals. They are key players in most of vertebrate developmental processes and so, implicated in several types of human congenital abnormalities, including holoprosoencephalia, and cancer(3-5). The most pleiotropic of vertebrate Hh molecules is Shh. It orchestrates dive ...
Chapter 6
... In protostomes as well as deuterostomes, the midline is an embryonic region that functions in patterning the adjacent nervous tissue (Sasai and De Robertis 1997; Menne et al. 1997). The ventral midline in insects (protostomes) is the region from which cells detach to form the ventrally located nerve ...
... In protostomes as well as deuterostomes, the midline is an embryonic region that functions in patterning the adjacent nervous tissue (Sasai and De Robertis 1997; Menne et al. 1997). The ventral midline in insects (protostomes) is the region from which cells detach to form the ventrally located nerve ...
shh
... Tal2 + cells are neuronal progenitor cells – Subset sim1 + V3 interneurons – May also give rise to other interneurons Tal2 – foxa2+ cells are non-neuronal Insect comparison – Both insect ML and vertebrate have Sim + interneurons ...
... Tal2 + cells are neuronal progenitor cells – Subset sim1 + V3 interneurons – May also give rise to other interneurons Tal2 – foxa2+ cells are non-neuronal Insect comparison – Both insect ML and vertebrate have Sim + interneurons ...
Mid-semester examination Developmental Biology (BSE652
... fate to the ventral cells of the neural tube. A) Describe how you will demonstrate that the floor plate fate is imparted to cells of the neural tube by the notochord. Start by choosing the model organism in which you will demonstrate this and then describe the experiment(s) you will perform to show ...
... fate to the ventral cells of the neural tube. A) Describe how you will demonstrate that the floor plate fate is imparted to cells of the neural tube by the notochord. Start by choosing the model organism in which you will demonstrate this and then describe the experiment(s) you will perform to show ...
Full Text
... there are several growth factors belonging to different families which are strongly produced in discrete regions of the embryo and affect the behaviour of blastodermal cells by activating gene pathways that finally lead to the production of different cell types. Several signaling molecules have been ...
... there are several growth factors belonging to different families which are strongly produced in discrete regions of the embryo and affect the behaviour of blastodermal cells by activating gene pathways that finally lead to the production of different cell types. Several signaling molecules have been ...
Neural Development
... The incredibly importnat and diverse functions of the human nervous system that range from senseation, perception, learning, memory, emotion, movement, thinking, and so much more all rely on the formation, organization, and connection of different types of nerve cells. ...
... The incredibly importnat and diverse functions of the human nervous system that range from senseation, perception, learning, memory, emotion, movement, thinking, and so much more all rely on the formation, organization, and connection of different types of nerve cells. ...
Sonic hedgehog
Sonic hedgehog is a protein that in humans is encoded by the SHH (""sonic hedgehog"") gene. Both the gene and the protein may also be found notated alternatively as ""Shh"".Sonic hedgehog is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and Indian hedgehog (IHH). SHH is the best studied ligand of the hedgehog signaling pathway. It plays a key role in regulating vertebrate organogenesis, such as in the growth of digits on limbs and organization of the brain. Sonic hedgehog is the best established example of a morphogen as defined by Lewis Wolpert's French flag model—a molecule that diffuses to form a concentration gradient and has different effects on the cells of the developing embryo depending on its concentration. SHH remains important in the adult. It controls cell division of adult stem cells and has been implicated in the development of some cancers.