rational drug design
... We take antibiotics to cure infection with bacteria. Antibiotics can work in a number of different ways: they can interfere with important bacterial enzymes or they might rupture bacterial cell membranes. We have probably all taken antibiotics at some time in our lives, but how many of us have taken ...
... We take antibiotics to cure infection with bacteria. Antibiotics can work in a number of different ways: they can interfere with important bacterial enzymes or they might rupture bacterial cell membranes. We have probably all taken antibiotics at some time in our lives, but how many of us have taken ...
Not just another hole in the wall: understanding intercellular protein
... autonomous proteins traffic to the cell membrane by hitchhiking on vesicles destined for particular plasma membrane subdomains associated with plasmodesmata. In support of this hypothesis, expression of antisense of LeRab11a in tomato led to plants with reduced apical dominance, determinate growth, ...
... autonomous proteins traffic to the cell membrane by hitchhiking on vesicles destined for particular plasma membrane subdomains associated with plasmodesmata. In support of this hypothesis, expression of antisense of LeRab11a in tomato led to plants with reduced apical dominance, determinate growth, ...
Title Gene Synthesis, Expression, and Mutagenesis of Zucchini
... encoding 182 amino acid long precursor stellacyanin from Cucumis sativas, and expressed 109 amino acid non-glycosylated Cu binding domain of that in E. coli (15). It is the only example of phytocyanin gene cloning. Comparison of the primary structure of mavicyanin with those of other cupredoxins sho ...
... encoding 182 amino acid long precursor stellacyanin from Cucumis sativas, and expressed 109 amino acid non-glycosylated Cu binding domain of that in E. coli (15). It is the only example of phytocyanin gene cloning. Comparison of the primary structure of mavicyanin with those of other cupredoxins sho ...
Glucagon receptor recycling: role of carboxyl terminus - AJP-Cell
... fate of internalized GR. Using both hamster hepatocytes and human embryonic kidney (HEK)-293 cells, we showed that internalized GR recycled to the plasma membrane within 30 – 60 min following stimulation of the cells with 100 nM glucagon. In HEK-293 cells and during recycling, GR colocalized with Ra ...
... fate of internalized GR. Using both hamster hepatocytes and human embryonic kidney (HEK)-293 cells, we showed that internalized GR recycled to the plasma membrane within 30 – 60 min following stimulation of the cells with 100 nM glucagon. In HEK-293 cells and during recycling, GR colocalized with Ra ...
Embryonic electronics - The Department of Computer Science
... resemble (albeit abstractly) their biological counterparts. We are dealing here with a ‘life-as-itcould-be’ approach, as opposed to a ‘life-as-we-know-it’ one (Langton, 1992); the reader should bear this in mind when encountering biological terms that have taken on new meanings (at least with respec ...
... resemble (albeit abstractly) their biological counterparts. We are dealing here with a ‘life-as-itcould-be’ approach, as opposed to a ‘life-as-we-know-it’ one (Langton, 1992); the reader should bear this in mind when encountering biological terms that have taken on new meanings (at least with respec ...
Cell potential and cloning
... Idaho Gem and Idaho Star are clones. They are two of three mules who were born in 2003 as the result of a cloning project at the University of Idaho and Utah State University. Leased from the University of Idaho for racing by Don Jacklin, an Idaho businessman, Gem and Star will make their first care ...
... Idaho Gem and Idaho Star are clones. They are two of three mules who were born in 2003 as the result of a cloning project at the University of Idaho and Utah State University. Leased from the University of Idaho for racing by Don Jacklin, an Idaho businessman, Gem and Star will make their first care ...
Organic Chemistry Notes Powerpoint
... 3. Which of the following substances is NOT a lipid? A. wax C. nucleic acids B. steroids D. triglycerides ...
... 3. Which of the following substances is NOT a lipid? A. wax C. nucleic acids B. steroids D. triglycerides ...
The Bcl-3 oncoprotein acts as a bridging factor between NF
... proteasome following ubiquitination (May and Ghosh, 1997). In contrast, no such posttranslational modifications have been described for Bcl-3, the only IkB-like protein that is not degraded after signaling and that is primarily localized in the nucleus. The cellular function of Bcl-3 remains largely ...
... proteasome following ubiquitination (May and Ghosh, 1997). In contrast, no such posttranslational modifications have been described for Bcl-3, the only IkB-like protein that is not degraded after signaling and that is primarily localized in the nucleus. The cellular function of Bcl-3 remains largely ...
Document
... With respect to the above question, Dyneins move material towards which end of the filament? (a) ...
... With respect to the above question, Dyneins move material towards which end of the filament? (a) ...
Lecture 18 slides - Rob Phillips` lab
... sensors, some of which we are conscious of, others of which we are not. Obvious examples – touch, hearing, vision, taste, smell Less obvious – sharks and the ampullae of Lorenzini – electrical detection. Sensors from pH to temperature to sugar. ...
... sensors, some of which we are conscious of, others of which we are not. Obvious examples – touch, hearing, vision, taste, smell Less obvious – sharks and the ampullae of Lorenzini – electrical detection. Sensors from pH to temperature to sugar. ...
SOD binds cell-adhesive peroxidase - Journal of Cell Science
... SDS-PAGE was performed in a 10% gel as described above under nonreducing conditions. After electrophoresis, the gel was soaked in transfer buffer (20 mM Tris, 150 mM glycine, pH 8.8) for 10 minutes and the proteins were transferred for 1 hour at 210 mA to nitrocellulose in the transfer buffer. The f ...
... SDS-PAGE was performed in a 10% gel as described above under nonreducing conditions. After electrophoresis, the gel was soaked in transfer buffer (20 mM Tris, 150 mM glycine, pH 8.8) for 10 minutes and the proteins were transferred for 1 hour at 210 mA to nitrocellulose in the transfer buffer. The f ...
Interaction between Cell Wall and Plasma Membrane via RGD Motif
... The fact that integrin- and vitronectin-like proteins existed in plasma membranes and cell walls, respectively, suggests that an interaction occurs between cell wall protein(s) and plasma membrane protein(s) mediated by RGD-motif. To evaluate such a hypothesis, a far-western blotting analysis was pe ...
... The fact that integrin- and vitronectin-like proteins existed in plasma membranes and cell walls, respectively, suggests that an interaction occurs between cell wall protein(s) and plasma membrane protein(s) mediated by RGD-motif. To evaluate such a hypothesis, a far-western blotting analysis was pe ...
Nervous System II Yellow
... When a neuron is not being disturbed (resting), it maintains a constant electrical charge across its membrane, where the cytoplasm is negatively charged, and the extracellular fluids are positively charged. These charges are reversed by stimuli, or outside forces. When this happens, the cytoplasm be ...
... When a neuron is not being disturbed (resting), it maintains a constant electrical charge across its membrane, where the cytoplasm is negatively charged, and the extracellular fluids are positively charged. These charges are reversed by stimuli, or outside forces. When this happens, the cytoplasm be ...
slides
... – prevent serotonin from being taken back up into neurons Both of these increase the level of serotonin, and thus (usually) improve mood Mood disorders are often triggered or exacerbated by environment—stress, life events Thus, scientists also look for genes that might impact response to stress as w ...
... – prevent serotonin from being taken back up into neurons Both of these increase the level of serotonin, and thus (usually) improve mood Mood disorders are often triggered or exacerbated by environment—stress, life events Thus, scientists also look for genes that might impact response to stress as w ...
Chapter 8. Movement across the Membrane
... Move from HIGH to LOW concentration through a protein channel passive transport no energy needed facilitated = with help ...
... Move from HIGH to LOW concentration through a protein channel passive transport no energy needed facilitated = with help ...
Code Questions Answers 1. Write the reactions of glycolysis
... Regulation of serum calcium level (Calcium homeostasis): Calcium homeostasis is a finely balanced process. The ionic calcium level is maintained by Vitamin D and hormones like Para thyroid hormone (PTH) and Calcitonin. (1 mark) Action of Vitamin D Increases the absorption of Ca (and phosphate) from ...
... Regulation of serum calcium level (Calcium homeostasis): Calcium homeostasis is a finely balanced process. The ionic calcium level is maintained by Vitamin D and hormones like Para thyroid hormone (PTH) and Calcitonin. (1 mark) Action of Vitamin D Increases the absorption of Ca (and phosphate) from ...
Preparation of enzymatically active recombinant class III
... • Class III HDACs can be readily purified as enzymatically active protein after overexpression in E. coli, generating a large amount of enzymatic deacetylase activity. • It has some limitations including the possible absence of regulatory cofactors and post-translation modifications. ...
... • Class III HDACs can be readily purified as enzymatically active protein after overexpression in E. coli, generating a large amount of enzymatic deacetylase activity. • It has some limitations including the possible absence of regulatory cofactors and post-translation modifications. ...
I-1 I. Introduction BIOCHEMISTRY = METABOLISM At first you may
... (A) The processing of foods to yield new cellular material. (B) The provision of energy to support all biological activities. For the survival of the species: (C) The biosynthesis of the nucleic acids (for the transmission of genetic information from parent to progeny). However conventional metaboli ...
... (A) The processing of foods to yield new cellular material. (B) The provision of energy to support all biological activities. For the survival of the species: (C) The biosynthesis of the nucleic acids (for the transmission of genetic information from parent to progeny). However conventional metaboli ...
07 Interneuronal connections
... – Causes the membrane to become more permeable to potassium and chloride ions – Leaves the charge on the inner surface more negative (flow of K+ out of the cytosol makes the interior more negative relative to the exterior of the membrane – Reduces the postsynaptic neuron’s ability to produce an acti ...
... – Causes the membrane to become more permeable to potassium and chloride ions – Leaves the charge on the inner surface more negative (flow of K+ out of the cytosol makes the interior more negative relative to the exterior of the membrane – Reduces the postsynaptic neuron’s ability to produce an acti ...
A system for functional analysis of Ebola virus glycoprotein
... densely arrayed spikes on the envelopes of both VSVDG*ResGP and VSVDG*-G virions, but VSVDG* virions were spikeless (Fig. 2A). Immunogold staining showed that a monoclonal antibody to ResGP bound to partially disrupted VSVDG*-ResGP virions (Fig. 2B Middle, at the upper right) but not to intact parti ...
... densely arrayed spikes on the envelopes of both VSVDG*ResGP and VSVDG*-G virions, but VSVDG* virions were spikeless (Fig. 2A). Immunogold staining showed that a monoclonal antibody to ResGP bound to partially disrupted VSVDG*-ResGP virions (Fig. 2B Middle, at the upper right) but not to intact parti ...
Colloids gels suspensions
... You are already familiar with several protein gels. Scrambled eggs, and yogurt are gels formed by proteins. And, of course, the words 'gel' and 'gelatin' both come from the Latin word for 'freeze', and gelatin is a protein gel. Water soluble proteins like the albumins in egg white are generally sphe ...
... You are already familiar with several protein gels. Scrambled eggs, and yogurt are gels formed by proteins. And, of course, the words 'gel' and 'gelatin' both come from the Latin word for 'freeze', and gelatin is a protein gel. Water soluble proteins like the albumins in egg white are generally sphe ...
Analysis of the Juxtamembrane Dileucine Motif in the Insulin Receptor
... To investigate the importance of the dileucine pair at positions 986 and 987 in the juxtamembrane domain of the insulin receptor, we mutated the LeuLeu residues to MetSer and AlaAla, and expressed the various recombinant receptors in NIH-3T3 cells. Metabolic labeling studies confirmed that the mutan ...
... To investigate the importance of the dileucine pair at positions 986 and 987 in the juxtamembrane domain of the insulin receptor, we mutated the LeuLeu residues to MetSer and AlaAla, and expressed the various recombinant receptors in NIH-3T3 cells. Metabolic labeling studies confirmed that the mutan ...
Modeling the Structural Consequences of BEST1 Missense Mutations
... Bestrophinopathies are a group of inherited retinal disorders primarily caused by point mutations scattered throughout the entire BEST1 gene. In humans, most of these sequence alterations lead to Best vitelliform macular dystrophy (BVMD), and in dogs cause cmr, a retinal phenotype modeling BVMD (Guz ...
... Bestrophinopathies are a group of inherited retinal disorders primarily caused by point mutations scattered throughout the entire BEST1 gene. In humans, most of these sequence alterations lead to Best vitelliform macular dystrophy (BVMD), and in dogs cause cmr, a retinal phenotype modeling BVMD (Guz ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.