Compressibility gives new insight into protein dynamics and enzyme
... state DHFRW NADPHWDHF is substituted by a complex DHFRWNADPHWMTX since the X-ray structures of both complexes are quite similar. Evidently, the £exibility of DHFR changes alternatively by binding or releasing the coenzyme and substrate: the transient state DHFRWNADPHWDHF is expected to be most £exib ...
... state DHFRW NADPHWDHF is substituted by a complex DHFRWNADPHWMTX since the X-ray structures of both complexes are quite similar. Evidently, the £exibility of DHFR changes alternatively by binding or releasing the coenzyme and substrate: the transient state DHFRWNADPHWDHF is expected to be most £exib ...
Chemosensory organs as models of neuronal synapses
... presynaptic, postsynaptic and glial entities composing both synapse types. In this article I propose that chemosensory synapses may serve as useful models of neuronal synapses, and consider the possibility that the two synapse types derive from a common ancestral structure. Neuronal synapses, the ph ...
... presynaptic, postsynaptic and glial entities composing both synapse types. In this article I propose that chemosensory synapses may serve as useful models of neuronal synapses, and consider the possibility that the two synapse types derive from a common ancestral structure. Neuronal synapses, the ph ...
Characterization of Rice Group 3 LEA Genes in Developmental
... Late embryogenesis abundant (LEA) proteins were first identified and characterized in cotton and wheat during the last stage of seed maturation (Galau and Hughes 1987). Up to 4 % of total cellular proteins during seed development are LEA proteins (Roberts et al. 1993). LEA proteins are grouped into ...
... Late embryogenesis abundant (LEA) proteins were first identified and characterized in cotton and wheat during the last stage of seed maturation (Galau and Hughes 1987). Up to 4 % of total cellular proteins during seed development are LEA proteins (Roberts et al. 1993). LEA proteins are grouped into ...
Motor protein control of ion flux is an early step in embryonic left
... from the way in which other species establish asymmetry; this would present its own problems, since then it would have to be explained how animals with such different early mechanisms all converge on the well-conserved left-sided expression of genes such as Nodal. The definitive answer to this quest ...
... from the way in which other species establish asymmetry; this would present its own problems, since then it would have to be explained how animals with such different early mechanisms all converge on the well-conserved left-sided expression of genes such as Nodal. The definitive answer to this quest ...
Bio-201-chapter-5-MEC
... • Enzymes that digest starch by hydrolyzing linkages can’t hydrolyze linkages in cellulose • Cellulose in human food passes through the digestive tract as insoluble fiber • Some microbes use enzymes to digest cellulose • Many herbivores, from cows to termites, have symbiotic relationships with ...
... • Enzymes that digest starch by hydrolyzing linkages can’t hydrolyze linkages in cellulose • Cellulose in human food passes through the digestive tract as insoluble fiber • Some microbes use enzymes to digest cellulose • Many herbivores, from cows to termites, have symbiotic relationships with ...
Neuron Structure and Function
... - open after about 1-2 msec of threshold depolarization - now K+ flows out of the cell and speeds the repolarization process - cause the hyperpolarization after the action potential - open K+ channels make the K+ permeability higher than at rest - membrane more negative on inside - hyperpolarization ...
... - open after about 1-2 msec of threshold depolarization - now K+ flows out of the cell and speeds the repolarization process - cause the hyperpolarization after the action potential - open K+ channels make the K+ permeability higher than at rest - membrane more negative on inside - hyperpolarization ...
The Copines, a Novel Class of C2 Domain-containing, Calcium
... extracting buffer again and sedimented as before, providing a second extract. In some cases a third extraction was performed, yielding about 20% additional protein. The extracts from the lipid vesicles were pooled and applied to a Poros Q anion exchange column (PerSeptive Biosystems, Cambridge, MA) ...
... extracting buffer again and sedimented as before, providing a second extract. In some cases a third extraction was performed, yielding about 20% additional protein. The extracts from the lipid vesicles were pooled and applied to a Poros Q anion exchange column (PerSeptive Biosystems, Cambridge, MA) ...
SpoIIQ Anchors Membrane Proteins on Both Sides of
... FIGURE 1. SpoIIE and SpoIIQ reside in a membrane complex. Immunoprecipitations using anti-GFP resin no more than a few faint bands were were performed on detergent-solubilized membrane fractions derived from B. subtilis cells at hour 2.5 of sporulation. Immunoprecipitates from a strain lacking GFP ( ...
... FIGURE 1. SpoIIE and SpoIIQ reside in a membrane complex. Immunoprecipitations using anti-GFP resin no more than a few faint bands were were performed on detergent-solubilized membrane fractions derived from B. subtilis cells at hour 2.5 of sporulation. Immunoprecipitates from a strain lacking GFP ( ...
BIOLOGY
... c) the use of hormones in negative feedback systems to achieve homeostasis in adult organisms d) items secreted in animal embryos used to help organize their development e) various receptors used to detect external signals to which the organism responds © 2014 Pearson Education, Inc. ...
... c) the use of hormones in negative feedback systems to achieve homeostasis in adult organisms d) items secreted in animal embryos used to help organize their development e) various receptors used to detect external signals to which the organism responds © 2014 Pearson Education, Inc. ...
Chapter 10 Roche Bio
... will reproduce until they come into contact with other cells. They respond by not growing. This demonstrates that controls on cell growth and division can be turned on and off. ...
... will reproduce until they come into contact with other cells. They respond by not growing. This demonstrates that controls on cell growth and division can be turned on and off. ...
BioExpress® 6000 Mammalian Cell Growth Media
... and Prof. Judith Klein-Seetharaman at the Goethe University in Frankfurt, Germany. The CIL media, labeled with either 15N or 13C; 15N Gly, Lys, Leu, Gln, Ser, Thr, Val and Trp, was used to express rhodopsin from the HEK293 mammalian cell system. ...
... and Prof. Judith Klein-Seetharaman at the Goethe University in Frankfurt, Germany. The CIL media, labeled with either 15N or 13C; 15N Gly, Lys, Leu, Gln, Ser, Thr, Val and Trp, was used to express rhodopsin from the HEK293 mammalian cell system. ...
A Conserved Family of Nuclear Proteins Containing
... several distinct proteins recognized by the antibodies. In 4-24 hr old embryos, some of these proteins could not be detected anymore (Figures 5g and 5h). This argues for a family of nuclear proteins defined by a common structural element, the H/C link of the Kr finger domain, and for de- ...
... several distinct proteins recognized by the antibodies. In 4-24 hr old embryos, some of these proteins could not be detected anymore (Figures 5g and 5h). This argues for a family of nuclear proteins defined by a common structural element, the H/C link of the Kr finger domain, and for de- ...
From Hans Selye`s discovery of biological stress to the
... mune, and visceral efferent limbs of the stress response. For instance brain CRF activates the sympathetic nervous system inducing the systemic release of catecholamines involved in the ”fight or flight” response. The locus coeruleus is also activated by CRF and its noradrenergic projections to the ...
... mune, and visceral efferent limbs of the stress response. For instance brain CRF activates the sympathetic nervous system inducing the systemic release of catecholamines involved in the ”fight or flight” response. The locus coeruleus is also activated by CRF and its noradrenergic projections to the ...
Section 3A Analysis on a Western Blot
... that recognizes the tag-specific antibody. (Secondary antibodies for indirect detection of primary antibodies are usually conjugated with POD or AP.) Suitable enzyme substrates for Western blotting must produce a signal on the membrane at the site of the enzymeconjugated antibody (and thus, the tagg ...
... that recognizes the tag-specific antibody. (Secondary antibodies for indirect detection of primary antibodies are usually conjugated with POD or AP.) Suitable enzyme substrates for Western blotting must produce a signal on the membrane at the site of the enzymeconjugated antibody (and thus, the tagg ...
Novel functions for Rab GTPases in multiple aspects of tumour
... Rab proteins are part of the large Ras superfamily of small GTPases. There are more than 60 members in humans and each of them is specifically localized to a subcellular membrane compartment, of which it controls the intracellular traffic [1]. Rab proteins cycle between an inactive cytosolic GDP-bou ...
... Rab proteins are part of the large Ras superfamily of small GTPases. There are more than 60 members in humans and each of them is specifically localized to a subcellular membrane compartment, of which it controls the intracellular traffic [1]. Rab proteins cycle between an inactive cytosolic GDP-bou ...
Chemosensory organs as models of neuronal synapses
... presynaptic, postsynaptic and glial entities composing both synapse types. In this article I propose that chemosensory synapses may serve as useful models of neuronal synapses, and consider the possibility that the two synapse types derive from a common ancestral structure. Neuronal synapses, the ph ...
... presynaptic, postsynaptic and glial entities composing both synapse types. In this article I propose that chemosensory synapses may serve as useful models of neuronal synapses, and consider the possibility that the two synapse types derive from a common ancestral structure. Neuronal synapses, the ph ...
Gene Section ALOX5 (arachidonate 5-lipoxygenase) Atlas of Genetics and Cytogenetics
... dehydration to the allylic epoxide 5(S)-trans-7,9-trans11,14-cis-eicosatetrenoic acid (leukotriene A4). The LTA4 intermediate is then converted to LTB4 by LTA4 hydrolase. LTB4 attracts leukocytes and are important for the inflammatory response. 5-LOX migrates to the nuclear membrane upon cellular ac ...
... dehydration to the allylic epoxide 5(S)-trans-7,9-trans11,14-cis-eicosatetrenoic acid (leukotriene A4). The LTA4 intermediate is then converted to LTB4 by LTA4 hydrolase. LTB4 attracts leukocytes and are important for the inflammatory response. 5-LOX migrates to the nuclear membrane upon cellular ac ...
The role of Cdc14 phosphatases in the control of cell division
... 3 Saito, R.M., Perreault, A., Peach, B., Satterlee, J.S. and van den Heuvel, S. (2004) The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans. Nat. Cell Biol. 6, 777–783 4 Mishima, M., Pavicic, V., Gruneberg, U., Nigg, E.A. and Glotzer, M. (2004) Cell cycle regulation of centr ...
... 3 Saito, R.M., Perreault, A., Peach, B., Satterlee, J.S. and van den Heuvel, S. (2004) The CDC-14 phosphatase controls developmental cell-cycle arrest in C. elegans. Nat. Cell Biol. 6, 777–783 4 Mishima, M., Pavicic, V., Gruneberg, U., Nigg, E.A. and Glotzer, M. (2004) Cell cycle regulation of centr ...
dead-end filtration of disrupted saccharomyces cerevisiae yeast
... The interior of the yeast cells (S. cerevisiae) is a rich source of bio-products (proteins, cytoplasmic enzymes, polysaccharides, etc.) valuable for different applications in biotechnology, brewing and food industry. Different steps are commonly present in extraction processes, including techniques ...
... The interior of the yeast cells (S. cerevisiae) is a rich source of bio-products (proteins, cytoplasmic enzymes, polysaccharides, etc.) valuable for different applications in biotechnology, brewing and food industry. Different steps are commonly present in extraction processes, including techniques ...
Endocrine Vivas
... How are the Ketone bodies produced and how are they metabolised? - Fatty acids (β oxidation) => acetyl-CoA => citric acid cycle => high output of energy (c.f. CHOs) - Occurs in the mitochondria in the liver and other tissues - Acetyl-CoA will condense => acetoacetyl-CoA (and aceyl-CoA + acetoacetyl- ...
... How are the Ketone bodies produced and how are they metabolised? - Fatty acids (β oxidation) => acetyl-CoA => citric acid cycle => high output of energy (c.f. CHOs) - Occurs in the mitochondria in the liver and other tissues - Acetyl-CoA will condense => acetoacetyl-CoA (and aceyl-CoA + acetoacetyl- ...
Complement Receptor Type 1 (CD35) Mediates - Bio
... Whereas the role of human CR2 in B cell activation is relatively well-established, much less is known about the exact function of CR1 (CD35). CR1 is a single-chain glycoprotein, which exists in four allotypes, the most common of them having a molecular mass of ⬃220 kDa and consisting of 30 short con ...
... Whereas the role of human CR2 in B cell activation is relatively well-established, much less is known about the exact function of CR1 (CD35). CR1 is a single-chain glycoprotein, which exists in four allotypes, the most common of them having a molecular mass of ⬃220 kDa and consisting of 30 short con ...
Signal transduction
Signal transduction occurs when an extracellular signaling molecule activates a specific receptor located on the cell surface or inside the cell. In turn, this receptor triggers a biochemical chain of events inside the cell, creating a response. Depending on the cell, the response alters the cell's metabolism, shape, gene expression, or ability to divide. The signal can be amplified at any step. Thus, one signaling molecule can cause many responses.