Modulating progenitor accumulation attenuates lung angiogenesis in a mouse model of asthma
... previously been shown to attenuate both eosinophilia and AHR in a mouse model of asthma [18]. In the present study AMD3100, given intranasally was administered either concurrently during the onset of disease or in a reversal/delayed treatment regimen once airway disease was established. Our data sho ...
... previously been shown to attenuate both eosinophilia and AHR in a mouse model of asthma [18]. In the present study AMD3100, given intranasally was administered either concurrently during the onset of disease or in a reversal/delayed treatment regimen once airway disease was established. Our data sho ...
The Cdk inhibitors p25rum1 and p40SIC1 are functional
... The first goal of these studies was to stablish if Cdk inhibitors from other organisms were able to rescue the phenotypes of fission yeast cells deleted for the rum1+ gene. Deletion of the rum1+ in the temperature sensitive cdc10-129 mutant background reduces the maximal temperature at which the cdc ...
... The first goal of these studies was to stablish if Cdk inhibitors from other organisms were able to rescue the phenotypes of fission yeast cells deleted for the rum1+ gene. Deletion of the rum1+ in the temperature sensitive cdc10-129 mutant background reduces the maximal temperature at which the cdc ...
OF PISUM SATIVUM L. (a) Source of Material
... Fig. 3). As time progressed these coalesced so that each cell contained several large vacuoles by the end of phase 2. This differentiation and expansion of the vacuolar system coincided with the expansion of the cells. The long axis of the plastids increased from about 3 fk to 8 fk during phase 2. T ...
... Fig. 3). As time progressed these coalesced so that each cell contained several large vacuoles by the end of phase 2. This differentiation and expansion of the vacuolar system coincided with the expansion of the cells. The long axis of the plastids increased from about 3 fk to 8 fk during phase 2. T ...
Fate of primitive streak cells
... transplantation of lacZ- or GFP-expressing transgenic cells and in situ labeling of cell populations. The micromanipulated embryos were examined after 24-48 hours of in vitro culture to determine the localization and number of transgenic or labeled cells in different tissues at the whole embryo and ...
... transplantation of lacZ- or GFP-expressing transgenic cells and in situ labeling of cell populations. The micromanipulated embryos were examined after 24-48 hours of in vitro culture to determine the localization and number of transgenic or labeled cells in different tissues at the whole embryo and ...
Activation of different myogenic pathways: myf-5 is
... with adjacent axial structures gave rise to a large number of βgal-positive (i.e. differentiated) cells; in contrast, very few βgal-positive cells were generated from UPM cultured in isolation. However, a good extent of differentiation occurred when UPM was cultured with adjacent lateral structures, ...
... with adjacent axial structures gave rise to a large number of βgal-positive (i.e. differentiated) cells; in contrast, very few βgal-positive cells were generated from UPM cultured in isolation. However, a good extent of differentiation occurred when UPM was cultured with adjacent lateral structures, ...
The Phenomen of Squamous Metaplasia in Benign and Malignant
... extension into the adjacent terminal ducts of the duct system. In contrast with single papilloma, where Sq.M is quite common, intraductal papillomatosis rarely presents Sq.M. Gynecomastia is a benign proliferative process of the male breast, characterized by an unencapsulated proliferation of ducts ...
... extension into the adjacent terminal ducts of the duct system. In contrast with single papilloma, where Sq.M is quite common, intraductal papillomatosis rarely presents Sq.M. Gynecomastia is a benign proliferative process of the male breast, characterized by an unencapsulated proliferation of ducts ...
The FRA1 kinesin contributes to cortical microtubule
... Both cortical microtubule and cell wall organization are essentially unaltered in fra1-5 The original characterization of FRA1 reported that microtubule organization in fra1-1 plants was similar to that of wild-type plants (Zhong et al., 2002). We confirmed this for fra1-5 in both roots and hypocoty ...
... Both cortical microtubule and cell wall organization are essentially unaltered in fra1-5 The original characterization of FRA1 reported that microtubule organization in fra1-1 plants was similar to that of wild-type plants (Zhong et al., 2002). We confirmed this for fra1-5 in both roots and hypocoty ...
The enhancement of histone H4 and H2A serine 1 phosphorylation
... 2000). Also, in vitro chromatin condensation assays using Xenopus extracts indicated that neither H3S10 phosphorylation nor the H3 N-terminal tail is required for mitotic chromatin condensation (de la Barre et al. 2001). Therefore, although H3S10 phosphorylation may play a role in chromosome condens ...
... 2000). Also, in vitro chromatin condensation assays using Xenopus extracts indicated that neither H3S10 phosphorylation nor the H3 N-terminal tail is required for mitotic chromatin condensation (de la Barre et al. 2001). Therefore, although H3S10 phosphorylation may play a role in chromosome condens ...
RNA SHAPE analysis in living cells
... RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2′-hydroxyl acylation analyzed by primer extension (SHA ...
... RNA structure has important roles in practically every facet of gene regulation, but the paucity of in vivo structural probes limits current understanding. Here we design, synthesize and demonstrate two new chemical probes that enable selective 2′-hydroxyl acylation analyzed by primer extension (SHA ...
Redistribution and differential extraction of soluble proteins in
... if the soluble proteins do not bind to any cellular structures, they would appear to be preferentially localized to compartments or volumes of cytoplasm from which they are least easily extracted. Furthermore, both sources of error could operate together. These errors could also occur even when cell ...
... if the soluble proteins do not bind to any cellular structures, they would appear to be preferentially localized to compartments or volumes of cytoplasm from which they are least easily extracted. Furthermore, both sources of error could operate together. These errors could also occur even when cell ...
Activation of different myogenic pathways: myf
... with adjacent axial structures gave rise to a large number of βgal-positive (i.e. differentiated) cells; in contrast, very few βgal-positive cells were generated from UPM cultured in isolation. However, a good extent of differentiation occurred when UPM was cultured with adjacent lateral structures, ...
... with adjacent axial structures gave rise to a large number of βgal-positive (i.e. differentiated) cells; in contrast, very few βgal-positive cells were generated from UPM cultured in isolation. However, a good extent of differentiation occurred when UPM was cultured with adjacent lateral structures, ...
1749-8104-8-5 - Cambridge Repository
... Pard3-GFP is not strongly polarized in neural rod cells at 15 hpf (Figure 2C), but by 18 hpf Pard3-GFP is distinctly localized to the midline of the rod (Figure 2D). This timing of Pard3-GFP polarization is unaffected by isochronic transplantation (Figure 2E,F). Therefore isochronic cells show norma ...
... Pard3-GFP is not strongly polarized in neural rod cells at 15 hpf (Figure 2C), but by 18 hpf Pard3-GFP is distinctly localized to the midline of the rod (Figure 2D). This timing of Pard3-GFP polarization is unaffected by isochronic transplantation (Figure 2E,F). Therefore isochronic cells show norma ...
Cytoskeletal control of plant cell shape: getting the fine points
... new cell wall material and membrane [29]. However, Factin has long been suspected to play one or more additional roles in tip growth. This notion has recently been strengthened by the observation that tip growth is considerably more sensitive to a variety of actin-disrupting treatments than is the ...
... new cell wall material and membrane [29]. However, Factin has long been suspected to play one or more additional roles in tip growth. This notion has recently been strengthened by the observation that tip growth is considerably more sensitive to a variety of actin-disrupting treatments than is the ...
Phloem imaging - Oxford Academic
... (Erni et al., 2009). Therefore, from the 1950s until the present day, transmission electron microscopy (TEM) has been the medium of choice when subcellular detail needs to be studied. However, TEM requires elaborate tissue preparation and sectioning and only static images of dynamic processes can be ...
... (Erni et al., 2009). Therefore, from the 1950s until the present day, transmission electron microscopy (TEM) has been the medium of choice when subcellular detail needs to be studied. However, TEM requires elaborate tissue preparation and sectioning and only static images of dynamic processes can be ...
Ca2 -Dependent Exocytosis in the Somata of Dorsal Root Ganglion
... To understand the role of soma release better, we asked: do the somata of vertebrate neurons undergo Ca21-dependent exocytosis under physiological conditions? If they do, what are the Ca21 requirements for the release? By combining membrane capacitance and Fura-2 measurements, we studied changes in ...
... To understand the role of soma release better, we asked: do the somata of vertebrate neurons undergo Ca21-dependent exocytosis under physiological conditions? If they do, what are the Ca21 requirements for the release? By combining membrane capacitance and Fura-2 measurements, we studied changes in ...
Mouse embryonic stem cells can differentiate via multiple
... or late states, or does it utilize an entirely distinct path (Fig. 1b)? And can DP converge fully to the same final state that is produced in embryos despite taking an alternative path (Fig. 1c)? These questions have been challenging to answer in part due to the high degree of heterogeneity in dir ...
... or late states, or does it utilize an entirely distinct path (Fig. 1b)? And can DP converge fully to the same final state that is produced in embryos despite taking an alternative path (Fig. 1c)? These questions have been challenging to answer in part due to the high degree of heterogeneity in dir ...
Cell Receptor To Promote B Cell Survival R Interacts and
... cultured WT and FcmR2/2 splenic B cells in the presence of antiIgM F(ab9)2 fragment, CD40L, or LPS. Consistent with our previous findings (19), FcmR2/2 splenic B cells showed decreased survival following a-IgM but not LPS stimulation (Fig. 1A, 1B). In addition, we found that FcmR deficiency did not ...
... cultured WT and FcmR2/2 splenic B cells in the presence of antiIgM F(ab9)2 fragment, CD40L, or LPS. Consistent with our previous findings (19), FcmR2/2 splenic B cells showed decreased survival following a-IgM but not LPS stimulation (Fig. 1A, 1B). In addition, we found that FcmR deficiency did not ...
Coombes JL, Han SJ, van Rooijen N, Raulet DH, Robey EA. 2012. Infection-induced regulation of natural killer cells by macrophages and collagen at the lymph node subcapsular sinus. Cell rep 2(1):124-135.
... observed that a substantial fraction of the slow-moving NK cells closely associated with second harmonic signals indicative of collagen fibers (Figure 3A; Movie S2). Antibody staining of lymph node tissue sections confirmed that these structures contained type I collagen (Figure 3B). Interactions be ...
... observed that a substantial fraction of the slow-moving NK cells closely associated with second harmonic signals indicative of collagen fibers (Figure 3A; Movie S2). Antibody staining of lymph node tissue sections confirmed that these structures contained type I collagen (Figure 3B). Interactions be ...
The Evolution of Robust Development and Homeostasis in Artificial
... consequence of the evolution of development. Using artificial evolution, we evolved cellular automata-based digital organisms that have distinct embryonic and homeostatic phases of development. Although these evolved organisms use a variety of strategies to maintain their form over time, organisms o ...
... consequence of the evolution of development. Using artificial evolution, we evolved cellular automata-based digital organisms that have distinct embryonic and homeostatic phases of development. Although these evolved organisms use a variety of strategies to maintain their form over time, organisms o ...
ECM and Drusen
... proteoglycan), laminin and nidogen. Also present in BrM are matricellular proteins and associated proteins. Matricellular proteins contribute to cell-matrix interactions and RPE cell responses and include thrombospondin 1, fibulins, TGF-beta (Bornstein and Sage 2002). Growth factors comprise one cla ...
... proteoglycan), laminin and nidogen. Also present in BrM are matricellular proteins and associated proteins. Matricellular proteins contribute to cell-matrix interactions and RPE cell responses and include thrombospondin 1, fibulins, TGF-beta (Bornstein and Sage 2002). Growth factors comprise one cla ...
Jan Rice - `Advanced wound assessment`
... • Recently, it was demonstrated that swab specimens obtained using Levine's technique and processed in the laboratory using quantitative procedures provided a reasonably accurate culture compared with that of wound tissue specimens processed in the same ...
... • Recently, it was demonstrated that swab specimens obtained using Levine's technique and processed in the laboratory using quantitative procedures provided a reasonably accurate culture compared with that of wound tissue specimens processed in the same ...
The Rice TAL Effector–Dependent Resistance
... rice growing regions of the world. During infection, X. oryzae pv oryzae strains deliver members of the large AvrBs3-related effector family into host cells via the bacterial type III secretion system (Yang and White, 2004). AvrBs3-like effectors, also referred to as transcription activator–like (TA ...
... rice growing regions of the world. During infection, X. oryzae pv oryzae strains deliver members of the large AvrBs3-related effector family into host cells via the bacterial type III secretion system (Yang and White, 2004). AvrBs3-like effectors, also referred to as transcription activator–like (TA ...
Subtype-specific regulation of equilibrative nucleoside transporters
... sites, has been identified, to date, in human cells [8]. Protein kinase CK2 is a serine/threonine protein kinase that is found in nearly all compartments of mammalian cells [11], and exists as a heterotetramer composed of two catalytic (α) subunits and two regulatory (β) subunits [12]. It appears to ...
... sites, has been identified, to date, in human cells [8]. Protein kinase CK2 is a serine/threonine protein kinase that is found in nearly all compartments of mammalian cells [11], and exists as a heterotetramer composed of two catalytic (α) subunits and two regulatory (β) subunits [12]. It appears to ...
Control of the number of cell division rounds in distinct tissues
... female nucleus. The amount of DNA after fertilization and the volume of cytoplasm in an intact egg are supposed as ‘2n’ and 1, respectively. The amount of DNA is ‘n’ when the female nucleus was removed. The volume of cytoplasm is 1/2 in half fragments. The N/C ratio and volume of egg fragments are i ...
... female nucleus. The amount of DNA after fertilization and the volume of cytoplasm in an intact egg are supposed as ‘2n’ and 1, respectively. The amount of DNA is ‘n’ when the female nucleus was removed. The volume of cytoplasm is 1/2 in half fragments. The N/C ratio and volume of egg fragments are i ...
Blood Stem Cell Activity Is Arrested by Th1
... Downloaded from http://www.jimmunol.org/ by guest on May 8, 2017 ...
... Downloaded from http://www.jimmunol.org/ by guest on May 8, 2017 ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.