Chapter 17
... cells (see Note 3). Simple modifications of this protocol can be used for assaying receptors in different types of native or engineered cells, including suspension cells (see Note 4). These modifications include the cell culture medium and time, initial seeding numbers of cells, cell synchronization ...
... cells (see Note 3). Simple modifications of this protocol can be used for assaying receptors in different types of native or engineered cells, including suspension cells (see Note 4). These modifications include the cell culture medium and time, initial seeding numbers of cells, cell synchronization ...
No Slide Title
... Types of rooting on stem cuttings (based on the type of roots that develop) • Preformed roots and latent root initials - present when the cutting is taken from the stem • Wound-induced roots - not present when the cutting is made (cutting the stem into pieces causes a wound reaction that stimulates ...
... Types of rooting on stem cuttings (based on the type of roots that develop) • Preformed roots and latent root initials - present when the cutting is taken from the stem • Wound-induced roots - not present when the cutting is made (cutting the stem into pieces causes a wound reaction that stimulates ...
Human B7-H3 binds to Triggering receptor expressed on myeloid
... Figure 3. B7-H3:TLT-2 ligation up-regulates cytokine production from T cells. (a) The expression levels of CD54, CD16/32, and B7-H3 on parental or hB7-H3/P815 were examined with PE-anti-CD54, biotin-anti-CD16/32, or biotin-anti-mouse/human B7-H3 mAb (MIH32), followed by streptavidin-PE. Representati ...
... Figure 3. B7-H3:TLT-2 ligation up-regulates cytokine production from T cells. (a) The expression levels of CD54, CD16/32, and B7-H3 on parental or hB7-H3/P815 were examined with PE-anti-CD54, biotin-anti-CD16/32, or biotin-anti-mouse/human B7-H3 mAb (MIH32), followed by streptavidin-PE. Representati ...
Plant Bioreactor Design
... anchorage-dependent cells in perfusion. • Patented double-screen Cell-Lift impeller for low shear and high oxygenation maximizes yields in microcarrier cultures. If foam accumulates it coalesces in the screened-in foam-inhibitor chamber at top of impeller and dissipates when forced through the scree ...
... anchorage-dependent cells in perfusion. • Patented double-screen Cell-Lift impeller for low shear and high oxygenation maximizes yields in microcarrier cultures. If foam accumulates it coalesces in the screened-in foam-inhibitor chamber at top of impeller and dissipates when forced through the scree ...
Melanoma cell lysate induces CCR7 expression and in vivo
... upper surface of the filter were mechanically removed. This last step allowed us to maintain the cells that were able to pass through the filter’s pore to the opposite side. Dry filters were further analysed using an inverted microscope (Leica DM IL, Wetzlar, Germany). Cells considered to have migra ...
... upper surface of the filter were mechanically removed. This last step allowed us to maintain the cells that were able to pass through the filter’s pore to the opposite side. Dry filters were further analysed using an inverted microscope (Leica DM IL, Wetzlar, Germany). Cells considered to have migra ...
Discovery and Characterization of Novel Small Molecule Inhibitors
... Antiproliferative Assays. The proliferation of human MDAMD-435 breast and PC-3 prostate cells was measured by our previously described fluorescence-based assay with a Cellomics ArrayScan II (Vogt et al., 2002). In brief, cells (1000 per well) were plated and treated in two 384-well collagen-coated d ...
... Antiproliferative Assays. The proliferation of human MDAMD-435 breast and PC-3 prostate cells was measured by our previously described fluorescence-based assay with a Cellomics ArrayScan II (Vogt et al., 2002). In brief, cells (1000 per well) were plated and treated in two 384-well collagen-coated d ...
Epithelial enhancement of connective tissue
... extracellular matrix (ECM) (Kosher, Lash & Minor, 1973; Kosher & Church, 1975; Kosher & Lash, 1975; Lash & Vasan, 1978) enhance cartilage differentiation in vitro. In these later studies, cartilage differentiation was monitored largely by [35S] sulphate incorporation into glycosaminoglycans or prote ...
... extracellular matrix (ECM) (Kosher, Lash & Minor, 1973; Kosher & Church, 1975; Kosher & Lash, 1975; Lash & Vasan, 1978) enhance cartilage differentiation in vitro. In these later studies, cartilage differentiation was monitored largely by [35S] sulphate incorporation into glycosaminoglycans or prote ...
Glossary of Lay Terms for use in Consent Documents A
... individual cells that are genetically identical as a result of dividing mitotically from a single progenitor cell ...
... individual cells that are genetically identical as a result of dividing mitotically from a single progenitor cell ...
Methods for fluorescence qualification of your cloning methods
... = green image thumbnail. Top line of thumbnails = cell candidate 1. Bottom line of thumbnails = cell candidate 2. For every microplate imaged, an overview of the result is displayed as a microplate map, highlighting where fluorescent objects have been detected. This overview will enable a quick visu ...
... = green image thumbnail. Top line of thumbnails = cell candidate 1. Bottom line of thumbnails = cell candidate 2. For every microplate imaged, an overview of the result is displayed as a microplate map, highlighting where fluorescent objects have been detected. This overview will enable a quick visu ...
Memory CD4+ T cells: beyond “helper” functions
... Questions and future challenges While the work by McKinstry et al. provides novel insights into cellular mechanisms by which memory CD4 + T cells contribute to immune protection against influenza, several questions remain. Areas that warrant further study include the PRR-independent protection elici ...
... Questions and future challenges While the work by McKinstry et al. provides novel insights into cellular mechanisms by which memory CD4 + T cells contribute to immune protection against influenza, several questions remain. Areas that warrant further study include the PRR-independent protection elici ...
10/13/2013 Sutureless Amniotic Membranes: When and How to Use Them
... All data on file in regard to testing for the tissue Do Not use: Areas with active or latent infection Disorder that would create unacceptable risk of post op complications Not to be used in eyes with GLC drainage devices or blebs ...
... All data on file in regard to testing for the tissue Do Not use: Areas with active or latent infection Disorder that would create unacceptable risk of post op complications Not to be used in eyes with GLC drainage devices or blebs ...
Meiosis
... The larger a cell becomes, the more demands the cell places on its DNA, and the harder it is to supply with nutrients. ...
... The larger a cell becomes, the more demands the cell places on its DNA, and the harder it is to supply with nutrients. ...
Effects of herbal preparation EquigaardTM on
... Fig. 1A depicts that proliferation of cells were significantly inhibited by the addition of ethanol extracts of Equiguard. As little as 1 @/ml of extract was sufficient to cause a 30% reduction in cell growth after a 72-h incubation. Increase in Equiguard to 3 pllml resulted in a proportional greate ...
... Fig. 1A depicts that proliferation of cells were significantly inhibited by the addition of ethanol extracts of Equiguard. As little as 1 @/ml of extract was sufficient to cause a 30% reduction in cell growth after a 72-h incubation. Increase in Equiguard to 3 pllml resulted in a proportional greate ...
Establishment of an experimental system allowing immobilization of
... was found equally in the medium and the cell wall. In the same year, it was reported that another hybrid protein consisting of the E7 protein of human papillomavirus type 16 and the M6 surface protein from Streptococcus pyogenes could be anchored on the surface of S. gordinii (Pozzi et al., 1992). D ...
... was found equally in the medium and the cell wall. In the same year, it was reported that another hybrid protein consisting of the E7 protein of human papillomavirus type 16 and the M6 surface protein from Streptococcus pyogenes could be anchored on the surface of S. gordinii (Pozzi et al., 1992). D ...
Epithelial cells retain junctions during mitosis
... these junctions were originally defined by their ultrastructural appearance, and electron microscopy is necessary to confirm the persistence of junctional structure. Mitotic cells in culture were, therefore, examined by electron microscopy in order to confirm the presence of intercellular junctions ...
... these junctions were originally defined by their ultrastructural appearance, and electron microscopy is necessary to confirm the persistence of junctional structure. Mitotic cells in culture were, therefore, examined by electron microscopy in order to confirm the presence of intercellular junctions ...
The Fundamental Unit of Life Introduction The cells that make up our
... They may even digest away the entire damaged or dead cell containing them Question (4): Why are peroxisomes mostly found in kidney and liver cells? Answer: Peroxisomes contain powerful oxidative enzymes, which specialize in carrying out certain oxidative reactions, in order to remove toxic substance ...
... They may even digest away the entire damaged or dead cell containing them Question (4): Why are peroxisomes mostly found in kidney and liver cells? Answer: Peroxisomes contain powerful oxidative enzymes, which specialize in carrying out certain oxidative reactions, in order to remove toxic substance ...
Technical Information Sheet No.15
... composition of medium. In order not to neglect any typical morphological changes arising during the growth cycle, it is also important to examine cultures of different age up to several days. 5. To use only purified agar brands (such as Oxoid Agar No.1, Difco Bacto Agar, and others) since normal aga ...
... composition of medium. In order not to neglect any typical morphological changes arising during the growth cycle, it is also important to examine cultures of different age up to several days. 5. To use only purified agar brands (such as Oxoid Agar No.1, Difco Bacto Agar, and others) since normal aga ...
Step A - Cloudfront.net
... • As a group, quietly discuss each question and agree upon one correct answer. The group with the most correct answers will win. ...
... • As a group, quietly discuss each question and agree upon one correct answer. The group with the most correct answers will win. ...
Review guide – Trimester 1 Finals General Biology – 2012 Chapter
... atom’s position on the periodic table? What are characteristics of ionic compounds? Where do we find ions and ionic compounds in living systems? How are covalent bonds formed? How many electrons make up a covalent bond? Why do some atoms form covalent and others form ionic bonds? Distinguish between ...
... atom’s position on the periodic table? What are characteristics of ionic compounds? Where do we find ions and ionic compounds in living systems? How are covalent bonds formed? How many electrons make up a covalent bond? Why do some atoms form covalent and others form ionic bonds? Distinguish between ...
Cell Structure and Function
... Specialized structures that perform important cellular functions ...
... Specialized structures that perform important cellular functions ...
Part 1 - Jobworks Biology
... Like mitochondria, plastids contain their own DNA. Therefore, according to endosymbiotic theory, plastids may also have evolved from ancient, freeliving prokaryotes that invaded larger prokaryotic cells. If so, they allowed early eukaryotes to make food and produce oxygen. Organization of Cells Cel ...
... Like mitochondria, plastids contain their own DNA. Therefore, according to endosymbiotic theory, plastids may also have evolved from ancient, freeliving prokaryotes that invaded larger prokaryotic cells. If so, they allowed early eukaryotes to make food and produce oxygen. Organization of Cells Cel ...
Lesson (1) Cell theory
... Cell: The building unit of living organisms which can carries out all vital processes inside living organisms. Cell theory: A theory which states that all living organisms consist of one or more cells, cell is the basic functional and structural unit of living organisms, and that cells are produced ...
... Cell: The building unit of living organisms which can carries out all vital processes inside living organisms. Cell theory: A theory which states that all living organisms consist of one or more cells, cell is the basic functional and structural unit of living organisms, and that cells are produced ...
Spatial and temporal changes in the expression of fibroglycan
... mAb 10H4 that had been coupled to CNBr-activated Sepharose 4B (2 mg/ml). After an overnight incubation at 4˚C, the 10H4Sepharose beads and bound materials were extensively washed with TTBS and then with heparitinase buffer (Lories et al., 1987). Half of the washed beads was treated with 1 mIU of hep ...
... mAb 10H4 that had been coupled to CNBr-activated Sepharose 4B (2 mg/ml). After an overnight incubation at 4˚C, the 10H4Sepharose beads and bound materials were extensively washed with TTBS and then with heparitinase buffer (Lories et al., 1987). Half of the washed beads was treated with 1 mIU of hep ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.