Medaka haploid embryonic stem cells are susceptible to Singapore
... materials, suggesting continuity of the assembly intermediate with cellular membrane compartments. At stage II, empty hexagonal capsids were apparent. At stage III, the assembly process was complete, leading to the production of mature virus particles. Similar stages have been described for African ...
... materials, suggesting continuity of the assembly intermediate with cellular membrane compartments. At stage II, empty hexagonal capsids were apparent. At stage III, the assembly process was complete, leading to the production of mature virus particles. Similar stages have been described for African ...
Thiazolidinediones Inhibit the Expression of
... M. Lavau (INSERM, Paris); and human neuroblastoma SK-N-MC cells were purchased from American Tissue Culture Collection. Both adipose cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) in the presence of 10% CO2, and SK-N-MC were cultured in minimal essential medium in 5% CO2. All ...
... M. Lavau (INSERM, Paris); and human neuroblastoma SK-N-MC cells were purchased from American Tissue Culture Collection. Both adipose cell lines were cultured in Dulbecco’s modified Eagle’s medium (DMEM) in the presence of 10% CO2, and SK-N-MC were cultured in minimal essential medium in 5% CO2. All ...
A Class of Human Proteins that Deliver Functional
... expression. We incubated the six NSHP-Cre fusion proteins with floxed tdTomato BSR cells, and again in all six cases, observed expression of the tdTomato reporter gene both by live-cell fluorescence microscopy (Figure 2A) and by flow cytometry (Figure 2B). For three of the human proteins (c-Jun, HBE ...
... expression. We incubated the six NSHP-Cre fusion proteins with floxed tdTomato BSR cells, and again in all six cases, observed expression of the tdTomato reporter gene both by live-cell fluorescence microscopy (Figure 2A) and by flow cytometry (Figure 2B). For three of the human proteins (c-Jun, HBE ...
Cell Membrane
... configuration. This means that the hydrophobic regions find ways to remove themselves from water, while the hydrophilic regions interact with water. ...
... configuration. This means that the hydrophobic regions find ways to remove themselves from water, while the hydrophilic regions interact with water. ...
02 Mitosis and Cytokinesis
... During anaphase, sister chromatids separate and the centromeres divide. The sister chromatids are pulled apart by the shortening of the spindle fibers. This is like reeling in a fish by shortening the fishing line. One sister chromatid moves to one pole of the cell, and the other sister chromatid mo ...
... During anaphase, sister chromatids separate and the centromeres divide. The sister chromatids are pulled apart by the shortening of the spindle fibers. This is like reeling in a fish by shortening the fishing line. One sister chromatid moves to one pole of the cell, and the other sister chromatid mo ...
X007/13/02
... the cells to differentiate. Analysis was carried out on the levels of specific marker molecules whose presence is associated with either self-renewal or differentiation. Results are shown in Figures 3A and 3B. 2A: Effect of knockout on growth rate ...
... the cells to differentiate. Analysis was carried out on the levels of specific marker molecules whose presence is associated with either self-renewal or differentiation. Results are shown in Figures 3A and 3B. 2A: Effect of knockout on growth rate ...
Vacuoles
... • Stores waste products so the rest of the cell is protected from contamination • Stores enzymes that break down macromolecules and detoxifying foreign substances • Slowly breaks down waste products into small pieces that cannot hurt the cell • Holds quantities of water or food ...
... • Stores waste products so the rest of the cell is protected from contamination • Stores enzymes that break down macromolecules and detoxifying foreign substances • Slowly breaks down waste products into small pieces that cannot hurt the cell • Holds quantities of water or food ...
P19 embryonal carcinoma cells
... the differentiated cells which develop. With drug-induced differentiation of Pig cells the spectrum of cell types is more limited than with spontaneously differentiating embryonal carcinoma and embryonic stem cells, but in no case does a single differentiated cell type develop. On the positive side, ...
... the differentiated cells which develop. With drug-induced differentiation of Pig cells the spectrum of cell types is more limited than with spontaneously differentiating embryonal carcinoma and embryonic stem cells, but in no case does a single differentiated cell type develop. On the positive side, ...
Nanotechnology is a highly promising and exciting cross
... their retention in cells and tissues, since it is believed that there is a threshold for the length of a fibre that is critical for induction of adverse biological effects (Hoet et al., 2004). The fact that the size of the particles itself can be a factor in direct toxicity and pathology is extreme ...
... their retention in cells and tissues, since it is believed that there is a threshold for the length of a fibre that is critical for induction of adverse biological effects (Hoet et al., 2004). The fact that the size of the particles itself can be a factor in direct toxicity and pathology is extreme ...
The Lymphatic System
... The flow of lymph 1-way flow from tissue towards heart Lymph arises from extracellular fluid Lymph capillaries flow into (→) Lymphatic vessels →Through lymph nodes → Lymph trunks → Lymph ducts → Subclavian veins → → heart ...
... The flow of lymph 1-way flow from tissue towards heart Lymph arises from extracellular fluid Lymph capillaries flow into (→) Lymphatic vessels →Through lymph nodes → Lymph trunks → Lymph ducts → Subclavian veins → → heart ...
Recombinant human GM-CSF
... and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblast, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation ...
... and is a key factor in differentiation pathways leading form stem cells. GMCSF is produced by several cell types as monocytes, fibroblast, endothelial cells and T- Lymphocytes in response to a number of inflammatory mediators present in the hemopoietic environment and peripheral site of inflammation ...
New method for the analysis of cell cycle
... Collection (Rockville, MD), were maintained in RPMI1640 medium (Gibco, Grand Island, NY) supplemented with 10% fetal calf serum (Gibco) and 2 mM L-glutamine (Sigma Chemical Co., St. Louis, MO). The experiments were performed on cells during their exponential phase of growth, as described elsewhere ( ...
... Collection (Rockville, MD), were maintained in RPMI1640 medium (Gibco, Grand Island, NY) supplemented with 10% fetal calf serum (Gibco) and 2 mM L-glutamine (Sigma Chemical Co., St. Louis, MO). The experiments were performed on cells during their exponential phase of growth, as described elsewhere ( ...
Cytoskeleton Reorganization and Cytokine Production of
... stimulate the immune functions and cytokine production in macrophages and T-lymphocytes. Accordingly, the RAW 264.7 murine macrophage cell line was used to assess the effects of Bifidobacterium on the proliferation and cytoskeleton reorganization of the cells. Cytokine production after exposure to B ...
... stimulate the immune functions and cytokine production in macrophages and T-lymphocytes. Accordingly, the RAW 264.7 murine macrophage cell line was used to assess the effects of Bifidobacterium on the proliferation and cytoskeleton reorganization of the cells. Cytokine production after exposure to B ...
Columbus County Schools Science Curriculum Guide SUBJECT
... the quote. Sir Isaac Newton once said, “If I have seen further, it is because I was standing on the shoulders of giants.” Students should write down their own interpretation of the quote and how it applies to the scientists that contributed to cell theory. Ask the students to share their thoughts ab ...
... the quote. Sir Isaac Newton once said, “If I have seen further, it is because I was standing on the shoulders of giants.” Students should write down their own interpretation of the quote and how it applies to the scientists that contributed to cell theory. Ask the students to share their thoughts ab ...
ABSORPTION DEGRADATION OF MONO-Si AND POLY
... Throughout the history of solar cell development, an efficiency increase has been a major priority because a low efficiency is one of the chief disadvantages in Photovoltaic (PV) industries [1-3]. Such improvement includes the development of a better model for increasing the absorbance of the solar ...
... Throughout the history of solar cell development, an efficiency increase has been a major priority because a low efficiency is one of the chief disadvantages in Photovoltaic (PV) industries [1-3]. Such improvement includes the development of a better model for increasing the absorbance of the solar ...
Collagen Self-Assembly Mechanisms
... To establish an experimental setup that will support fibrillogenesis To monitor and alter variables in experiment to determine the best environmental conditions for fibrillogenesis To replicate experimental data in computational modeling derived from research data ...
... To establish an experimental setup that will support fibrillogenesis To monitor and alter variables in experiment to determine the best environmental conditions for fibrillogenesis To replicate experimental data in computational modeling derived from research data ...
From skin to the treatment of diseases the possibilities of iPS cell
... the same or other gene families lead also to fully reprogrammed cells, e.g. Sox1 or Sox3 can substitute for Sox2; Klf2, Klf5 or Esrrb can substitute for Klf4, and c-Myc can be substituted by N-Myc and L-Myc (21–23). In addition, Wernig and colleagues (24) could show that c-Myc is not strictly requir ...
... the same or other gene families lead also to fully reprogrammed cells, e.g. Sox1 or Sox3 can substitute for Sox2; Klf2, Klf5 or Esrrb can substitute for Klf4, and c-Myc can be substituted by N-Myc and L-Myc (21–23). In addition, Wernig and colleagues (24) could show that c-Myc is not strictly requir ...
Expression of a constitutively activated form of protein kinase B (c
... demonstrated to activate the p70 S6 kinase [S], an insulinresponsive enzyme that regulates the translation of mRNAs that encode proteins involved in cell cycle control [8]. The potential role of PKB in insulin-dependent cellular responses is still uncertain. Differentiation of 3T3-Ll preadipose fibr ...
... demonstrated to activate the p70 S6 kinase [S], an insulinresponsive enzyme that regulates the translation of mRNAs that encode proteins involved in cell cycle control [8]. The potential role of PKB in insulin-dependent cellular responses is still uncertain. Differentiation of 3T3-Ll preadipose fibr ...
Role of Cytoskeleton in Controlling the Disorder Strength of Cellular
... theory according to which a signal in one dimension arising due to multiple interferences of light waves within a sample of weak refractive index is sensitive to any length scale of refractive index fluctuations. Because the optical refractive index is linearly related to the local mass density of m ...
... theory according to which a signal in one dimension arising due to multiple interferences of light waves within a sample of weak refractive index is sensitive to any length scale of refractive index fluctuations. Because the optical refractive index is linearly related to the local mass density of m ...
Growth and Controlled Morphogenesis in Pea Root
... the cambial regionof anwIAA-treatedroot at the growthof thecallus tissise.-It was apparentquite 5thday. The calluswas establishedon agar medium early that the yeast extract was essential for the containing1 g./l. yeastextractand 10-5M 2,4-D, maintainedgrowthof thecallus in cultureand that effectin t ...
... the cambial regionof anwIAA-treatedroot at the growthof thecallus tissise.-It was apparentquite 5thday. The calluswas establishedon agar medium early that the yeast extract was essential for the containing1 g./l. yeastextractand 10-5M 2,4-D, maintainedgrowthof thecallus in cultureand that effectin t ...
Diffusion and Cell Size Introduction
... relationship between cell size and the diffusion of substances across the cell membrane. Diffusion is the spontaneous movement of a substance from high to low concentration. It is how many substances naturally move from where there is more to where there less: such as the smell of perfume moving acr ...
... relationship between cell size and the diffusion of substances across the cell membrane. Diffusion is the spontaneous movement of a substance from high to low concentration. It is how many substances naturally move from where there is more to where there less: such as the smell of perfume moving acr ...
Hyperosmotic stress-induced actin filament reorganization in leaf
... order to release cells. The resulting cell suspension was then ®ltered through a 200 mm mesh to remove unmacerated tissue. The cell suspension was mildly centrifuged at 500 rpm for 10 min in a benchtop clinical centrifuge. The supernatant containing cell debris was discarded and the loose pellet was ...
... order to release cells. The resulting cell suspension was then ®ltered through a 200 mm mesh to remove unmacerated tissue. The cell suspension was mildly centrifuged at 500 rpm for 10 min in a benchtop clinical centrifuge. The supernatant containing cell debris was discarded and the loose pellet was ...
The role of cell cycle control mechanisms in regulated and
... transcription factors, signal transducers and cell cycle regulators. These oncogenes often have similar functions, and contribute to tumour cell self-sufficiency for growth, division and survival. (Adamson, 1987) Growth factors as oncogene products ...
... transcription factors, signal transducers and cell cycle regulators. These oncogenes often have similar functions, and contribute to tumour cell self-sufficiency for growth, division and survival. (Adamson, 1987) Growth factors as oncogene products ...
PDF
... along the x and y axes, assuming the outgrowth to be circular. Preliminary experiments in which giant-cell numbers obtained from counts of nuclei by phase-contrast microscopy were compared with those obtained from the same outgrowths after fixation and staining indicated that results based on the tw ...
... along the x and y axes, assuming the outgrowth to be circular. Preliminary experiments in which giant-cell numbers obtained from counts of nuclei by phase-contrast microscopy were compared with those obtained from the same outgrowths after fixation and staining indicated that results based on the tw ...
Methods of Enumeration of Microorganisms
... conditions that permit microbial reproduction so that colonies develop that can be seen without the aid of a microscope. It is assumed that each bacterial colony arises from an individual cell that has undergone cell division. Therefore, by counting the number of colonies and accounting for the dilu ...
... conditions that permit microbial reproduction so that colonies develop that can be seen without the aid of a microscope. It is assumed that each bacterial colony arises from an individual cell that has undergone cell division. Therefore, by counting the number of colonies and accounting for the dilu ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.