Immunoglobulin detection
... a precipitating substrate will form a colored spot at the location of the secreting cell, which can be viewed and counted in a microscope or, preferably, in an ELISpot reader. While this procedure is simple and straightforward, it does have certain weaknesses, which are related to the antigen. To ob ...
... a precipitating substrate will form a colored spot at the location of the secreting cell, which can be viewed and counted in a microscope or, preferably, in an ELISpot reader. While this procedure is simple and straightforward, it does have certain weaknesses, which are related to the antigen. To ob ...
Methods of Enumeration of Microorganisms
... conditions that permit microbial reproduction so that colonies develop that can be seen without the aid of a microscope. It is assumed that each bacterial colony arises from an individual cell that has undergone cell division. Therefore, by counting the number of colonies and accounting for the dilu ...
... conditions that permit microbial reproduction so that colonies develop that can be seen without the aid of a microscope. It is assumed that each bacterial colony arises from an individual cell that has undergone cell division. Therefore, by counting the number of colonies and accounting for the dilu ...
Regulation of epidermal cell fate in Arabidopsis roots
... epidermis cell fate. Here, we describe this regulatory network and the importance of the multiple ...
... epidermis cell fate. Here, we describe this regulatory network and the importance of the multiple ...
Chemoattraction of macrophages by secretory molecules derived
... sequences and structures, and their ribonucleolytic activity regulates gene expression at the mRNA level [6]. To date, 13 human protein sequences have been found to be similar to bovine pancreatic RNaseA [7-10]. The 19 arginine and 2 lysine residues in the primary sequence of ECP account for its hig ...
... sequences and structures, and their ribonucleolytic activity regulates gene expression at the mRNA level [6]. To date, 13 human protein sequences have been found to be similar to bovine pancreatic RNaseA [7-10]. The 19 arginine and 2 lysine residues in the primary sequence of ECP account for its hig ...
Dermatopathology: An abridged compendium of words
... Citation: Hookerman BJ. Dermatopathology: An abridged compendium of words. A discussion of them and opinions about them. Introduction and Part 1. Dermatol Pract Conc. 2013;3(2):4. http://dx.doi.org/10.5826/dpc.0302a04. Copyright: ©2013 Hookerman. This is an open-access article distributed under the ...
... Citation: Hookerman BJ. Dermatopathology: An abridged compendium of words. A discussion of them and opinions about them. Introduction and Part 1. Dermatol Pract Conc. 2013;3(2):4. http://dx.doi.org/10.5826/dpc.0302a04. Copyright: ©2013 Hookerman. This is an open-access article distributed under the ...
class 9 biology chapter- 1 fundamental unit of life introductory
... varies greatly depending on the size of the organism. Many cells group together to form tissues, many tissues join together to form organ, many organs form organ system and finally all systems make up an organism, hence called structural unit of life. A cell is able to live and perform the various f ...
... varies greatly depending on the size of the organism. Many cells group together to form tissues, many tissues join together to form organ, many organs form organ system and finally all systems make up an organism, hence called structural unit of life. A cell is able to live and perform the various f ...
Biolistic Particle Delivery Systems Brochure - Bio-Rad
... and the helium pressure can be selected to optimally penetrate different targets with minimal damage. Mammalian cells do not need to be trypsinized to remove them from their support when biolistic delivery is used, and thus cell morphology is maintained. Even intracellular organelles have been succe ...
... and the helium pressure can be selected to optimally penetrate different targets with minimal damage. Mammalian cells do not need to be trypsinized to remove them from their support when biolistic delivery is used, and thus cell morphology is maintained. Even intracellular organelles have been succe ...
Lymphocyte homing in the immune system
... between peripheral lymphoid organs. Specific retention mechanisms ensure that only mature lymphocytes leave the thymus and bone-marrow and homing mechanisms facilitate the entry of these naive cells into peripheral or secondary lymphoid organs such as the spleen and lymph nodes. Following an encount ...
... between peripheral lymphoid organs. Specific retention mechanisms ensure that only mature lymphocytes leave the thymus and bone-marrow and homing mechanisms facilitate the entry of these naive cells into peripheral or secondary lymphoid organs such as the spleen and lymph nodes. Following an encount ...
Cellular polarity, mitotic synchrony and axes of
... tion, however, requires that the cells themselves be polarized. Polarization precedes blastocyst formation in mammals and gastrulation in Drosophila. Within an epithelium, cells must be polarized along an initial, apico-basal, plane or they could not remain associated in a sheet (Gubb, 1993). This r ...
... tion, however, requires that the cells themselves be polarized. Polarization precedes blastocyst formation in mammals and gastrulation in Drosophila. Within an epithelium, cells must be polarized along an initial, apico-basal, plane or they could not remain associated in a sheet (Gubb, 1993). This r ...
Visualization of cell structure in situ by atomic force microscopy
... producing images of cells by scanning the surface. The images are of similar resolution to those generated by ultrastructural approaches. In fact, recent studies have shown that intranuclear 32 nm in diameter ribonucleoprotein particles -known as Lacandonia granules because they were first described ...
... producing images of cells by scanning the surface. The images are of similar resolution to those generated by ultrastructural approaches. In fact, recent studies have shown that intranuclear 32 nm in diameter ribonucleoprotein particles -known as Lacandonia granules because they were first described ...
cell sorting in the presence of cytochalasin b
... within 1 day of culture under the conditions employed in this experiment . (b) Aggregate from experimental culture (cytochalasin B concentration is 10 µg/ml) . Although the aggregate is compact, observable sorting of heart and pigmented retinal cells has not occurred . X 230 . was no apparent sortin ...
... within 1 day of culture under the conditions employed in this experiment . (b) Aggregate from experimental culture (cytochalasin B concentration is 10 µg/ml) . Although the aggregate is compact, observable sorting of heart and pigmented retinal cells has not occurred . X 230 . was no apparent sortin ...
A COMPARISON OF THE CYTOPLASMIC CHANGES INDUCED IN
... In evaluation of the effects of different types of radiation, test objects have been nearly as varied as methods of irradiation, Ascaris ova, chick fibroblasts, salamander cornea, and many others being used. In the present study we are attempting to follow not only the effect of radiation on various ...
... In evaluation of the effects of different types of radiation, test objects have been nearly as varied as methods of irradiation, Ascaris ova, chick fibroblasts, salamander cornea, and many others being used. In the present study we are attempting to follow not only the effect of radiation on various ...
Wade histoid Leprosy: histological and
... and containing long, well-stained and aligned bacilli (along the longer cell axis). The cells are arranged in an interconnected structure, sometimes resembling a fibrous histiocytic tumour. In this form, the lesion has well-defined limits and can be surrounded by a pseudocapsule. The infiltrate cons ...
... and containing long, well-stained and aligned bacilli (along the longer cell axis). The cells are arranged in an interconnected structure, sometimes resembling a fibrous histiocytic tumour. In this form, the lesion has well-defined limits and can be surrounded by a pseudocapsule. The infiltrate cons ...
Nucleic Acids
... Hydrolysis – Addition of a water molecule to a polymer to break a bond within a polymer to form the monomers ...
... Hydrolysis – Addition of a water molecule to a polymer to break a bond within a polymer to form the monomers ...
Low Intensity and Frequency Pulsed
... complete and resilient recovery. Fueling this recent interest is the fact that extremely lowfrequency and low-intensity pulsed electromagnetic fields (PEMFs) have been shown to be innocuous, possibly even beneficial [4], [6]–[7], to normal cell types. On the other hand, certain malignant cell classe ...
... complete and resilient recovery. Fueling this recent interest is the fact that extremely lowfrequency and low-intensity pulsed electromagnetic fields (PEMFs) have been shown to be innocuous, possibly even beneficial [4], [6]–[7], to normal cell types. On the other hand, certain malignant cell classe ...
PDF
... Distribution offibronectin (FN) As a prerequisite to understanding the possible role of FN in morphogenetic movements, its spatial distribution was determined before and during gastrulation. Specific antibodies directed against amphibian FN were applied either to sections or whole-mount specimens. L ...
... Distribution offibronectin (FN) As a prerequisite to understanding the possible role of FN in morphogenetic movements, its spatial distribution was determined before and during gastrulation. Specific antibodies directed against amphibian FN were applied either to sections or whole-mount specimens. L ...
RMV 04
... MDBK cells were grown to confluence on specially prepared glass lamellas (10×20 mm). They were inoculated with the CpHV-1 strain E/CH (105.5 CCID50) and after 1, 2, 4, 5, 6, 12, 24, 36, and 48 hours, they were fixed for 20 minutes in cold acetone (-20°C). Then cells were equilibrated in PBS pH 7.6, ...
... MDBK cells were grown to confluence on specially prepared glass lamellas (10×20 mm). They were inoculated with the CpHV-1 strain E/CH (105.5 CCID50) and after 1, 2, 4, 5, 6, 12, 24, 36, and 48 hours, they were fixed for 20 minutes in cold acetone (-20°C). Then cells were equilibrated in PBS pH 7.6, ...
The SPA2 Protein of Yeast Localizes to Sites of Cell Growth
... cells, i.e., cells that have given birth to one or more cells, bud in an axial pattern similar to that of haploids. New daughter cells, however, bud in a polar fashion, with the new bud forming opposite the old birth scar. The molecular mechanism that determines cell polarity is not understood at pr ...
... cells, i.e., cells that have given birth to one or more cells, bud in an axial pattern similar to that of haploids. New daughter cells, however, bud in a polar fashion, with the new bud forming opposite the old birth scar. The molecular mechanism that determines cell polarity is not understood at pr ...
Unit 3 Cell Str Func
... Quiz – Cells #2 3. Why does it have to be a LIPID layer that makes up the cell membrane? 4. Why does it have to be a BILAYER (2 layers) for the cell membrane? ...
... Quiz – Cells #2 3. Why does it have to be a LIPID layer that makes up the cell membrane? 4. Why does it have to be a BILAYER (2 layers) for the cell membrane? ...
2016_01_27_quiz_key
... 1. You are a rodent endocrinologist. Your patient, Algernon, has been diagnosed with hyperthyroidism based on elevated thyroxine (T4) levels in his blood. Describe two additional tests/observations/analyses that you could do to rule in or rule out specific possible causes of this hyperthyroidism. So ...
... 1. You are a rodent endocrinologist. Your patient, Algernon, has been diagnosed with hyperthyroidism based on elevated thyroxine (T4) levels in his blood. Describe two additional tests/observations/analyses that you could do to rule in or rule out specific possible causes of this hyperthyroidism. So ...
Independent Project - Classroom
... regrow diseased organs, or restore cells lost to age, disease, or trauma? Answering these questions has been the source of countless hours of research and experimental study for over a hundred years, and while extensive knowledge has been produced on this topic, many questions are still left to be a ...
... regrow diseased organs, or restore cells lost to age, disease, or trauma? Answering these questions has been the source of countless hours of research and experimental study for over a hundred years, and while extensive knowledge has been produced on this topic, many questions are still left to be a ...
No Slide Title
... INSULIN ACTS TO STORE NUTRIENTS. (carbohydrates, fats and protein) It is the only hormone that does this. A lack of insulin is fatal. ...
... INSULIN ACTS TO STORE NUTRIENTS. (carbohydrates, fats and protein) It is the only hormone that does this. A lack of insulin is fatal. ...
Motility of Escherichia coli cells in clusters formed by chemotactic aggregation Nikhil Mittal*
... addition of 12 l of 5 mM fumarate was found to be very similar to that of the parental strain. To prepare liquid cultures, individual colonies were picked from LB agar plates and grown to stationary phase in M9 glycerol medium as described in ref. 9. For the GFP-expressing cells, 50 g兾ml ampicilin ...
... addition of 12 l of 5 mM fumarate was found to be very similar to that of the parental strain. To prepare liquid cultures, individual colonies were picked from LB agar plates and grown to stationary phase in M9 glycerol medium as described in ref. 9. For the GFP-expressing cells, 50 g兾ml ampicilin ...
Marking cell lineages in living tissues
... in three axially adjacent pericycle files when several transverse divisions in single or in axial pairs of pericycle cells are followed by tangential periclinal divisions of an inner pair of derivatives resulting in the inner layer (IL) and outer layer (OL) of LRP cells (Figure 3a). When the marked ...
... in three axially adjacent pericycle files when several transverse divisions in single or in axial pairs of pericycle cells are followed by tangential periclinal divisions of an inner pair of derivatives resulting in the inner layer (IL) and outer layer (OL) of LRP cells (Figure 3a). When the marked ...
Flow Cytometry - From Discovery to Clinical Analysis | Charles River
... endpoint added to preclinical studies in order to assess the potential immunotoxicity effect of a drug. While changes in cell phenotypes are useful in some settings to characterize the immunotoxicity of different compounds, phenotypic analysis alone is often not a sensitive indicator of low-dose imm ...
... endpoint added to preclinical studies in order to assess the potential immunotoxicity effect of a drug. While changes in cell phenotypes are useful in some settings to characterize the immunotoxicity of different compounds, phenotypic analysis alone is often not a sensitive indicator of low-dose imm ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.