An indelible lineage marker for Xenopus using a
... Many important Xenopus transcription factors are strongly expressed in early development, but cease to be expressed a few hours later (Smith, 1993; Dawid, 1994). To determine the function of such genes, one important experimental route is to express them ectopically in a tissue where they are not no ...
... Many important Xenopus transcription factors are strongly expressed in early development, but cease to be expressed a few hours later (Smith, 1993; Dawid, 1994). To determine the function of such genes, one important experimental route is to express them ectopically in a tissue where they are not no ...
Chapter 1 The Study of Life/ Chapter 2
... organelles. Explain how cells and organisms maintain homeostasis. 7. Identify and describe animal and plant organelles. 8. List modes of motility for cells (flagella/cilia) 9. Explain how cells and organisms maintain homeostasis. 10. Contrast the movement of substances (osmosis/diffusion/active and ...
... organelles. Explain how cells and organisms maintain homeostasis. 7. Identify and describe animal and plant organelles. 8. List modes of motility for cells (flagella/cilia) 9. Explain how cells and organisms maintain homeostasis. 10. Contrast the movement of substances (osmosis/diffusion/active and ...
Cell cycle arrest and apoptosis induced by the coronavirus
... cytopathic effects (CPE) in Vero cells, IBV infection of H1299 cells showed rounding up and fusion of infected cells to form giant multinucleated syncytia, detachment of infected cells from the culture dish, and eventually cell lysis and death (Fig. 1a). The infected cells were further analyzed by W ...
... cytopathic effects (CPE) in Vero cells, IBV infection of H1299 cells showed rounding up and fusion of infected cells to form giant multinucleated syncytia, detachment of infected cells from the culture dish, and eventually cell lysis and death (Fig. 1a). The infected cells were further analyzed by W ...
Cellular programs for arbuscular mycorrhizal symbiosis
... from the roots of phosphate-deprived plants [7], activate AM fungal metabolism and this results in hyphal branching in proximity to the root [8,9]. Following contact with the root surface, the fungus forms a hyphopodium through which it penetrates the epidermis. Entry into the epidermal cell and su ...
... from the roots of phosphate-deprived plants [7], activate AM fungal metabolism and this results in hyphal branching in proximity to the root [8,9]. Following contact with the root surface, the fungus forms a hyphopodium through which it penetrates the epidermis. Entry into the epidermal cell and su ...
the cell cycle of symbiotic chlorella
... Chlorella algae symbiotic with green hydra did not arrest in G\ when cell division was blocked by host starvation, but appeared to cycle slowly through 5 phase. No distinct S/mitosis peak could be distinguished in algae from 1-day starved hosts, and if host starvation was prolonged, algal DNA conten ...
... Chlorella algae symbiotic with green hydra did not arrest in G\ when cell division was blocked by host starvation, but appeared to cycle slowly through 5 phase. No distinct S/mitosis peak could be distinguished in algae from 1-day starved hosts, and if host starvation was prolonged, algal DNA conten ...
O A RIGINAL RTICLE
... analysis many components has been characterized, but the major ones were thymoquinone (27.8%–57.0%), ρ-cymene (7.1%–15.5%), carvacrol(5.8%–11.6%), t-anethole (0.25%–2.3%), 4terpineol(2.0%–6.6%) and longifoline (1.0%–8.0%). The oil of NS is so beneficial due to its content of over 100 components such ...
... analysis many components has been characterized, but the major ones were thymoquinone (27.8%–57.0%), ρ-cymene (7.1%–15.5%), carvacrol(5.8%–11.6%), t-anethole (0.25%–2.3%), 4terpineol(2.0%–6.6%) and longifoline (1.0%–8.0%). The oil of NS is so beneficial due to its content of over 100 components such ...
Neova® Creme de la Copper Combats Photodamage with DNA
... Creme de la Copper combats the effects of photoaging on the skin. The treatment now combines Copper Peptide Complex technology with a new and powerful therapy—advanced DNA Repair Mitosomes, which contain an enzyme (OGG1 for 8-oxoguanine DNA glycosylase 1) produced from the common plant Arabidposis t ...
... Creme de la Copper combats the effects of photoaging on the skin. The treatment now combines Copper Peptide Complex technology with a new and powerful therapy—advanced DNA Repair Mitosomes, which contain an enzyme (OGG1 for 8-oxoguanine DNA glycosylase 1) produced from the common plant Arabidposis t ...
Hym-355 enhances neuron differentiation - Development
... Animals and culture conditions The 105 strain of Hydra magnipapillata was used for most experiments, and cultured as described previously (Sugiyama and Fujisawa, 1977). For some experiments ‘epithelial Hydra’, which are devoid of all cells of the interstitial cell lineage except for gland cells (Mar ...
... Animals and culture conditions The 105 strain of Hydra magnipapillata was used for most experiments, and cultured as described previously (Sugiyama and Fujisawa, 1977). For some experiments ‘epithelial Hydra’, which are devoid of all cells of the interstitial cell lineage except for gland cells (Mar ...
4)Cell wall
... mycetology. There are approximately 70,000 described species of fungi, however, it is estimated that there are over I million species of fungi on the planet. Some have Summary of Fungal characteristics The following attributes can be used to characterize those organisms that we classify as fungi: 1- ...
... mycetology. There are approximately 70,000 described species of fungi, however, it is estimated that there are over I million species of fungi on the planet. Some have Summary of Fungal characteristics The following attributes can be used to characterize those organisms that we classify as fungi: 1- ...
Migration Cues Induce Chromatin Alterations
... Directed cell migration is a fundamental property of both simple and complex organisms, which is necessary for the proper execution of various biological processes including foraging, embryonic development, immunity, tissue repair and homeostasis. Improper cell migration is an underlying cause of nu ...
... Directed cell migration is a fundamental property of both simple and complex organisms, which is necessary for the proper execution of various biological processes including foraging, embryonic development, immunity, tissue repair and homeostasis. Improper cell migration is an underlying cause of nu ...
Cell movements during epiboly and gastrulation in
... colored dyes, two sibling blastomeres; one underlying the other at the 64-cell stage (Fig. 2). The deeper cell generated a clone located deep in the DEL of the midblastula, and immediately underlying the clone originating from its superficial sib, as confirmed by direct inspection (Fig. 3A). DEL cel ...
... colored dyes, two sibling blastomeres; one underlying the other at the 64-cell stage (Fig. 2). The deeper cell generated a clone located deep in the DEL of the midblastula, and immediately underlying the clone originating from its superficial sib, as confirmed by direct inspection (Fig. 3A). DEL cel ...
HIF-2α phosphorylation by CK1δ promotes erythropoietin secretion
... cause any significant change in the in vitro phosphorylation by CK1δ (Fig. S2A). Taken together, our analysis identified residues Ser383 and Thr528 as the predominant CK1δ in vitro phosphorylation sites of human HIF-2α. The fact that these sites are also subjected to modification by the kinases pres ...
... cause any significant change in the in vitro phosphorylation by CK1δ (Fig. S2A). Taken together, our analysis identified residues Ser383 and Thr528 as the predominant CK1δ in vitro phosphorylation sites of human HIF-2α. The fact that these sites are also subjected to modification by the kinases pres ...
Embryonic stem cell differentiation and the analysis of mammalian
... *Address correspondence to: Dr. Peter Rathjen. Department of Molecular Biosciences, Adelaide University, Adelaide, 5005, South Australia, Australia. ...
... *Address correspondence to: Dr. Peter Rathjen. Department of Molecular Biosciences, Adelaide University, Adelaide, 5005, South Australia, Australia. ...
Stabilization Proliferative Potential and Telomere Length Increased
... shown). The hTERT⫹ CD8⫹ T cells maintained high levels of telomerase activity throughout the culture period (data not shown), suggesting that the increased proliferative potential might be due to effects of the exogenous hTERT gene on telomere length. To test this possibility, cryopreserved lymphocy ...
... shown). The hTERT⫹ CD8⫹ T cells maintained high levels of telomerase activity throughout the culture period (data not shown), suggesting that the increased proliferative potential might be due to effects of the exogenous hTERT gene on telomere length. To test this possibility, cryopreserved lymphocy ...
Herbicide Mode of Action - Montana IPM Center
... • Diagnosing herbicide injury • Prevent and manage herbicide resistance ...
... • Diagnosing herbicide injury • Prevent and manage herbicide resistance ...
In yeast, the pseudohyphal phenotype induced by isoamyl alcohol
... constructed containing a heterozygous CDC3/CDC3GFP fusion in the Σ1278b background, which shows the strongest response to IAA. Preliminary experiments established that 0.5% IAA was the optimum concentration to give a clear response without affecting viability. CMS2 was exposed to 0.5% IAA in liquid ...
... constructed containing a heterozygous CDC3/CDC3GFP fusion in the Σ1278b background, which shows the strongest response to IAA. Preliminary experiments established that 0.5% IAA was the optimum concentration to give a clear response without affecting viability. CMS2 was exposed to 0.5% IAA in liquid ...
Fate of Frog Virus 3 DNA Replicated in the Nucleus of Arginine
... Influence o f arginine starvation on the site and the kinetics o f D N A replication Non-confluent C H O cultures were pretreated overnight in alpha medium deficient in arginine, then infected with FV 3 and incubated either in alpha medium deprived of arginine or in complete medium. The newly synthe ...
... Influence o f arginine starvation on the site and the kinetics o f D N A replication Non-confluent C H O cultures were pretreated overnight in alpha medium deficient in arginine, then infected with FV 3 and incubated either in alpha medium deprived of arginine or in complete medium. The newly synthe ...
Ultrastructural Observations on Lumbar Spinal Cord
... Eight individuals with transected lumbar spinal cord, previously utilized for the analysis of behavioral recovery and histology at 29, 36 and 45 post-lesion were also utilized for the present electron microscopic study (Alibardi, 2010, 2014). The progressive time-points were selected to detect possi ...
... Eight individuals with transected lumbar spinal cord, previously utilized for the analysis of behavioral recovery and histology at 29, 36 and 45 post-lesion were also utilized for the present electron microscopic study (Alibardi, 2010, 2014). The progressive time-points were selected to detect possi ...
Unequal division in Saccharomyces cerevisiae and its implications
... For all experiments except a few of those reported in Fig. 6, cells grown in liquid medium were in YNB (18) and those grown on solid medium were on YNB containing 10 g/liter noble agar (Difco Laboratories, Detroit, Mich.). In a few experiments reported in Fig. 6, cells were grown in YNB containing s ...
... For all experiments except a few of those reported in Fig. 6, cells grown in liquid medium were in YNB (18) and those grown on solid medium were on YNB containing 10 g/liter noble agar (Difco Laboratories, Detroit, Mich.). In a few experiments reported in Fig. 6, cells were grown in YNB containing s ...
the single-cell preparation guide
... Density separation using Ficoll enriches B cells by shrinking red blood cells and separating them from white blood cells, leaving a concentrated population of peripheral blood mononuclear cells (PBMCs). The B cells are further enriched from the PBMC population by labeling with a fluorescent anti-CD1 ...
... Density separation using Ficoll enriches B cells by shrinking red blood cells and separating them from white blood cells, leaving a concentrated population of peripheral blood mononuclear cells (PBMCs). The B cells are further enriched from the PBMC population by labeling with a fluorescent anti-CD1 ...
ppt
... Embryos can develop outside the mother with high speed. High numbers of eggs useful for inducing mutations and ...
... Embryos can develop outside the mother with high speed. High numbers of eggs useful for inducing mutations and ...
Multipotency and Tissue-Specific Stem Cells
... As is the case for the HSCs, the skin itself, as well as isolated and cultured skin cells, can be transplanted back into animals and humans and contribute to new skin formation. Hair follicles were a source for long-term in vitro passaged populations that could be then implanted back and participate ...
... As is the case for the HSCs, the skin itself, as well as isolated and cultured skin cells, can be transplanted back into animals and humans and contribute to new skin formation. Hair follicles were a source for long-term in vitro passaged populations that could be then implanted back and participate ...
Apoptosis induced by prolonged exposure to odorants in cultured
... cultures subjected to diverse experimental conditions for 3 h are plotted in Fig. 4: (A) no odorant addition, (B) vehicle, (C) ethyl vanillin (EV), (D) LY83583 + ethyl vanillin, (E) isobutyrate (Isob), (F) LY83583 + isobutyrate, (G) camptothecin (positive control). As seen in Fig. 4, 94% of control ...
... cultures subjected to diverse experimental conditions for 3 h are plotted in Fig. 4: (A) no odorant addition, (B) vehicle, (C) ethyl vanillin (EV), (D) LY83583 + ethyl vanillin, (E) isobutyrate (Isob), (F) LY83583 + isobutyrate, (G) camptothecin (positive control). As seen in Fig. 4, 94% of control ...
Cytoplasmic calcium measurements in intact higher plant cells
... not possible to show that the apparent acceleration of streaming, after addition of verapamil, is statistically significant. The pattern of behaviour was, however, observed in most instances. During this period there was a small depolarization of the plasma membrane (Fig. 3). If no further drops of ...
... not possible to show that the apparent acceleration of streaming, after addition of verapamil, is statistically significant. The pattern of behaviour was, however, observed in most instances. During this period there was a small depolarization of the plasma membrane (Fig. 3). If no further drops of ...
The Lymphatic and Immune Systems
... Red bone marrow: site of lymphocyte origin Primary lymphoid organs: site of development of immunocompetence as B or T cells Secondary lymphoid organs: site of antigen encounter, and activation to become effector and memory B or T cells ...
... Red bone marrow: site of lymphocyte origin Primary lymphoid organs: site of development of immunocompetence as B or T cells Secondary lymphoid organs: site of antigen encounter, and activation to become effector and memory B or T cells ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.