Lenti-X™ Tet-On® Advanced Expression System User
... lentiviruses which are then used to transduce target cells. Cotransducing the regulator and response viruses into your target cells, creates a system that allows you to control the expression level of your gene of interest (GOI) by adjusting the concentration of the system’s inducer, doxycycline (Do ...
... lentiviruses which are then used to transduce target cells. Cotransducing the regulator and response viruses into your target cells, creates a system that allows you to control the expression level of your gene of interest (GOI) by adjusting the concentration of the system’s inducer, doxycycline (Do ...
The Lymphatic and Immune Systems
... Red bone marrow: site of lymphocyte origin Primary lymphoid organs: site of development of immunocompetence as B or T cells Secondary lymphoid organs: site of antigen encounter, and activation to become effector and memory B or T cells ...
... Red bone marrow: site of lymphocyte origin Primary lymphoid organs: site of development of immunocompetence as B or T cells Secondary lymphoid organs: site of antigen encounter, and activation to become effector and memory B or T cells ...
A theoretical analysis of the ephaptic feedback mechanism
... current densities one can obtain an estimate of the effective surface area for the distribution of the potassium and leak conductance on a horizontal cell complete with dendrites, axon and axon terminal. By assuming that horizontal cells lose their axons, axon terminals and 20 % of their dendrites o ...
... current densities one can obtain an estimate of the effective surface area for the distribution of the potassium and leak conductance on a horizontal cell complete with dendrites, axon and axon terminal. By assuming that horizontal cells lose their axons, axon terminals and 20 % of their dendrites o ...
regulation of cell growth by vitreous humour
... sources could inhibit tumour-induced neovascularization. The antiangiogenic activity of vitreous has also been demonstrated using the chick chorioallantoic membrane (CAM) assay (Lutty et al. 1983). In this study bovine retinal factor, a mitogen for endothelial cells, was used to stimulate neovascula ...
... sources could inhibit tumour-induced neovascularization. The antiangiogenic activity of vitreous has also been demonstrated using the chick chorioallantoic membrane (CAM) assay (Lutty et al. 1983). In this study bovine retinal factor, a mitogen for endothelial cells, was used to stimulate neovascula ...
Diapositive 1 - ORBi - Université de Liège
... EMT (epithelial-to-mesenchymal transition) is a process characterized by the loss of epithelial properties and the gain of mesenchymal properties. Among the transcription factors involved in EMT, SIP1 is known as a transcriptional repressor of epithelial genes, including E-cadherin. It has been show ...
... EMT (epithelial-to-mesenchymal transition) is a process characterized by the loss of epithelial properties and the gain of mesenchymal properties. Among the transcription factors involved in EMT, SIP1 is known as a transcriptional repressor of epithelial genes, including E-cadherin. It has been show ...
High throughput quantitative reverse transcription PCR assays
... tumourigenesis, drug resistance and recurrence of this disease, are enriched in the cancer stem cell-like side population (SP). Cancer testis antigens (CTA) are attractive targets for immunotherapy because they are widely expressed in cancers but only in limited types of normal tissues. We designed ...
... tumourigenesis, drug resistance and recurrence of this disease, are enriched in the cancer stem cell-like side population (SP). Cancer testis antigens (CTA) are attractive targets for immunotherapy because they are widely expressed in cancers but only in limited types of normal tissues. We designed ...
The role of T cells in Osteoporosis - International Journal of Clinical
... inflammatory conditions, lymphocytes also secrete a number of inhibitory molecules that directly inhibit OC formation, including OPG, IL-4, IL-10, IL-13, and IFN-γ. In addition to cytokines, many chemokines are directly or indirectly involved in regulating bone loss under pathological conditions, su ...
... inflammatory conditions, lymphocytes also secrete a number of inhibitory molecules that directly inhibit OC formation, including OPG, IL-4, IL-10, IL-13, and IFN-γ. In addition to cytokines, many chemokines are directly or indirectly involved in regulating bone loss under pathological conditions, su ...
Chapter 3 - Cell Structure and Function
... gives them a relatively large ratio of surface area to volume, facilitating the transfer of substances. The shapes of cells may vary, and a cell’s shape generally suits its function. • Cells are diverse, but all cells contain a plasma membrane, cytoplasm, ribosomes, and DNA. • Prokaryotic cells are ...
... gives them a relatively large ratio of surface area to volume, facilitating the transfer of substances. The shapes of cells may vary, and a cell’s shape generally suits its function. • Cells are diverse, but all cells contain a plasma membrane, cytoplasm, ribosomes, and DNA. • Prokaryotic cells are ...
A novel role for 14–3–3s in regulating epithelial
... cells exhibited a dramatically impaired metastatic phenotype (Fig. 1C). Collectively, these data argue that restoration of 14–3–3s function does not impact primary tumor growth, but significantly impairs ErbB2-induced breast cancer metastasis. One possible explanation for these observations is that ...
... cells exhibited a dramatically impaired metastatic phenotype (Fig. 1C). Collectively, these data argue that restoration of 14–3–3s function does not impact primary tumor growth, but significantly impairs ErbB2-induced breast cancer metastasis. One possible explanation for these observations is that ...
The Physiology of the Distal Tubules and Collecting Ducts
... • Cortical and outer medullary collecting ducts are made up of two different cell types. • Principal cells are responsible for water and sodium reabsorption as well as potassium secretion, and intercalated cells are involved in acid-base regulation. • Na+ reabsorption in principal cells is linked to ...
... • Cortical and outer medullary collecting ducts are made up of two different cell types. • Principal cells are responsible for water and sodium reabsorption as well as potassium secretion, and intercalated cells are involved in acid-base regulation. • Na+ reabsorption in principal cells is linked to ...
Effect of Butyric Acid on Lung-colonizing Ability
... alterations in mammalian cells that are relevant to reverse trans formation and induce differentiation of a variety of cell lines (1419). Butyric acid and its neutralized salt, sodium butyrate, have also been shown to cause similar changes in mammalian cells (14, 20-24). With these observations and ...
... alterations in mammalian cells that are relevant to reverse trans formation and induce differentiation of a variety of cell lines (1419). Butyric acid and its neutralized salt, sodium butyrate, have also been shown to cause similar changes in mammalian cells (14, 20-24). With these observations and ...
Megf10 regulates the progression of the satellite cell myogenic
... 3 and 4, and M-cadherin, have been shown to be expressed in quiescent satellite cells, which allows for easy immunological identification (Holterman and Rudnicki, 2005). In normal resting adult skeletal muscle, satellite cells are found in a mitotically quiescent state but can be induced to enter th ...
... 3 and 4, and M-cadherin, have been shown to be expressed in quiescent satellite cells, which allows for easy immunological identification (Holterman and Rudnicki, 2005). In normal resting adult skeletal muscle, satellite cells are found in a mitotically quiescent state but can be induced to enter th ...
Transport of Colloidal Particles from Small Blood Vessels Correlated
... and into the rat femoral vein. The tissue was embedded in Epon 812. Transport of particles during the preovulatory period across the capillary walls of the ovarian Graafian follicle The capillaries of the theca interna of the adult estrus rabbit are continuous without fenestrae or pores. Junctions b ...
... and into the rat femoral vein. The tissue was embedded in Epon 812. Transport of particles during the preovulatory period across the capillary walls of the ovarian Graafian follicle The capillaries of the theca interna of the adult estrus rabbit are continuous without fenestrae or pores. Junctions b ...
Name of presentation - Annual Unither Nanomedical
... with green arrows. It is evident that there is a preferential uptake of targeted nanoparticles to the LNCaP C4-2 cell line. ...
... with green arrows. It is evident that there is a preferential uptake of targeted nanoparticles to the LNCaP C4-2 cell line. ...
BCL-6 regulates chemokine gene transcription in macrophages
... BCL-6–deficient (BCL-6–/–) mice have shown that BCL-6 is essential for the formation of germinal centers and for a normal antibody response10,11. BCL-6–/– mice have also revealed, unexpectedly, that BCL-6 is major negative regulator of T helper cell subset 2 (TH2) differentiation and TH2-type inflam ...
... BCL-6–deficient (BCL-6–/–) mice have shown that BCL-6 is essential for the formation of germinal centers and for a normal antibody response10,11. BCL-6–/– mice have also revealed, unexpectedly, that BCL-6 is major negative regulator of T helper cell subset 2 (TH2) differentiation and TH2-type inflam ...
Local interactions shape plant cells
... vesicles access to the cell boundary and promote regional expansion. (e) Dissection of the molecular mechanisms underlying shaping of single pavement cells and interdigitating growth of neighbouring pavement cells reveals that the creation of a fine F- actin mesh and a dense cytoskeletal region depe ...
... vesicles access to the cell boundary and promote regional expansion. (e) Dissection of the molecular mechanisms underlying shaping of single pavement cells and interdigitating growth of neighbouring pavement cells reveals that the creation of a fine F- actin mesh and a dense cytoskeletal region depe ...
De novo fatty acid synthesis controls the fate between regulatory T
... glycolytic pathway is an active, lineage-decisive step that is required for the development of effector T (Teff ) cells of the TH1, TH2 and TH17 lineages4. Hence, in the absence of mTOR, or after treatment with the mTOR-specific inhibitor rapamycin, the development of Teff cells is greatly diminishe ...
... glycolytic pathway is an active, lineage-decisive step that is required for the development of effector T (Teff ) cells of the TH1, TH2 and TH17 lineages4. Hence, in the absence of mTOR, or after treatment with the mTOR-specific inhibitor rapamycin, the development of Teff cells is greatly diminishe ...
Amino Acid Metabolism of NovikoÃ-FHepatoma
... toneally (IP) into recipient rats. The animals were used for experiments after a period of 5-7 days, at which time the testes were cyanotic and the tumor, together with a considerable amount of serosanguineous fluid, could be palpated in the abdomen. Rats were killed by a sharp blow on the head and ...
... toneally (IP) into recipient rats. The animals were used for experiments after a period of 5-7 days, at which time the testes were cyanotic and the tumor, together with a considerable amount of serosanguineous fluid, could be palpated in the abdomen. Rats were killed by a sharp blow on the head and ...
Exosome-Depleted FBS Using Beckman Coulter Centrifugation:
... supplement to nearly all cell culture conditions. As the field of exosome research continues to expand, it is becoming increasingly important to standardize methods for FBS depletion in experiments utilizing cell culture. Commercially available depleted FBS is currently more than double the cost tha ...
... supplement to nearly all cell culture conditions. As the field of exosome research continues to expand, it is becoming increasingly important to standardize methods for FBS depletion in experiments utilizing cell culture. Commercially available depleted FBS is currently more than double the cost tha ...
Chapter 7: A View of the Cell
... 2. The cell is the basic unit of organization of organisms. Although organisms can become very large and complex, such as humans, dogs, and trees, the cell remains the simplest, most basic component of any organism. 3. All cells come from preexisting cells. Before the cell theory, no one knew how ce ...
... 2. The cell is the basic unit of organization of organisms. Although organisms can become very large and complex, such as humans, dogs, and trees, the cell remains the simplest, most basic component of any organism. 3. All cells come from preexisting cells. Before the cell theory, no one knew how ce ...
Cotyledon cells of Vigna mungo seedlings use at least two distinct
... interacts with vacuoles to be degraded by -amylase. A cotyledon of a day 3 seedling is composed of heterologous cells with respect to the amount of reserves in the cells. Cells positioned near a vascular bundle (VB) were filled with storage materials (Fig. 4 A, region I), the reserves were degraded ...
... interacts with vacuoles to be degraded by -amylase. A cotyledon of a day 3 seedling is composed of heterologous cells with respect to the amount of reserves in the cells. Cells positioned near a vascular bundle (VB) were filled with storage materials (Fig. 4 A, region I), the reserves were degraded ...
Differential requirement for OBF-1 during antibody
... We examined the cell-surface phenotypes of OBF-1/ and heterozygous control cells in more detail, staining simultaneously for Syndecan-1 and other markers known to change during ASC differentiation. In control cultures, Syndecan-1hi ASC lost the expression of CD25, Fas, and FcRII, but Syndecan-1in ...
... We examined the cell-surface phenotypes of OBF-1/ and heterozygous control cells in more detail, staining simultaneously for Syndecan-1 and other markers known to change during ASC differentiation. In control cultures, Syndecan-1hi ASC lost the expression of CD25, Fas, and FcRII, but Syndecan-1in ...
72 2. INTRODUCTION: THE ROLE OF ONCOGENES IN SIGNAL
... the subversion of these pathways by oncogenes has proven critical in unraveling the biochemical factors leading to cellular transformation. One such line of investigation has been study of the effects of transforming p21Ras on plateletderived growth factor type-beta receptor (PDGF-betaR) signaling. ...
... the subversion of these pathways by oncogenes has proven critical in unraveling the biochemical factors leading to cellular transformation. One such line of investigation has been study of the effects of transforming p21Ras on plateletderived growth factor type-beta receptor (PDGF-betaR) signaling. ...
Structure-Function Approach Identifies a COOH
... for proteolytic activation of heparanase (12–14, 20) is experimentally bypassed by a single-chain gene construct (21). In this protein variant, the linker segment was replaced by three glycineserine repeats (GS3), resulting in constitutively active heparanase enzyme (21). Apart of the well-studied c ...
... for proteolytic activation of heparanase (12–14, 20) is experimentally bypassed by a single-chain gene construct (21). In this protein variant, the linker segment was replaced by three glycineserine repeats (GS3), resulting in constitutively active heparanase enzyme (21). Apart of the well-studied c ...
Cotyledon cells of Vigna mungo seedlings use at
... were intact (Fig. 4 B). The electron density of the PSV became low when the degradation of proteins started (Fig. 4 C). In the same cells, a membranous structure surrounding SGs was observed, and some regions with low electron density (LED) were found (Fig. 4 C). The LED area around the SG was enlar ...
... were intact (Fig. 4 B). The electron density of the PSV became low when the degradation of proteins started (Fig. 4 C). In the same cells, a membranous structure surrounding SGs was observed, and some regions with low electron density (LED) were found (Fig. 4 C). The LED area around the SG was enlar ...
Tissue engineering
Tissue engineering is the use of a combination of cells, engineering and materials methods, and suitable biochemical and physicochemical factors to improve or replace biological functions. While it was once categorized as a sub-field of biomaterials, having grown in scope and importance it can be considered as a field in its own right.While most definitions of tissue engineering cover a broad range of applications, in practice the term is closely associated with applications that repair or replace portions of or whole tissues (i.e., bone, cartilage, blood vessels, bladder, skin, muscle etc.). Often, the tissues involved require certain mechanical and structural properties for proper functioning. The term has also been applied to efforts to perform specific biochemical functions using cells within an artificially-created support system (e.g. an artificial pancreas, or a bio artificial liver). The term regenerative medicine is often used synonymously with tissue engineering, although those involved in regenerative medicine place more emphasis on the use of stem cells or progenitor cells to produce tissues.