Cell movements during epiboly and gastrulation in
... passage of the pipette through the EVL was accompanied by a rise in voltage of up to 40 mV; the extracellular space surrounding DEL cells is at a positive potential relative to the bath (Bennett and Trinkaus, 1970). Upon intracellular penetration of a DEL cell, we then observed the expected shift to ...
... passage of the pipette through the EVL was accompanied by a rise in voltage of up to 40 mV; the extracellular space surrounding DEL cells is at a positive potential relative to the bath (Bennett and Trinkaus, 1970). Upon intracellular penetration of a DEL cell, we then observed the expected shift to ...
Exocytosis and cell polarity in plants exocyst and recycling domains
... Fig. 2 Role of lipid metabolism in exocytotic vesicle formation. (a) Hypothetical mechanism of trans-Golgi network (TGN)-to-plasma membrane carrier (TPC) formation, based on the mammalian model. Differences in the molecular shapes between phosphatidylinositol-4phosphate (PtdIns4P) (formed by RabA4b- ...
... Fig. 2 Role of lipid metabolism in exocytotic vesicle formation. (a) Hypothetical mechanism of trans-Golgi network (TGN)-to-plasma membrane carrier (TPC) formation, based on the mammalian model. Differences in the molecular shapes between phosphatidylinositol-4phosphate (PtdIns4P) (formed by RabA4b- ...
Lecture 10 golgi
... cells and from 10-20 in plant cells. Usually equally spaced in the stack, separated from each other by thin layers of intercisternal cytoplasm. Cisternae may be flat but are often curved. Golgi complex has a distinct polarity, the two poles are called cis face and trans face, which act respect ...
... cells and from 10-20 in plant cells. Usually equally spaced in the stack, separated from each other by thin layers of intercisternal cytoplasm. Cisternae may be flat but are often curved. Golgi complex has a distinct polarity, the two poles are called cis face and trans face, which act respect ...
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... this particular complex would normally prevent ES cells from differentiating into primitive endoderm by repressing the trigger factor, Gata6. Nanog is an NK-2 class homeobox transcription factor that is expressed throughout the pluripotent cells of the ICM. As overexpression of Nanog in mouse ES cel ...
... this particular complex would normally prevent ES cells from differentiating into primitive endoderm by repressing the trigger factor, Gata6. Nanog is an NK-2 class homeobox transcription factor that is expressed throughout the pluripotent cells of the ICM. As overexpression of Nanog in mouse ES cel ...
How is pluripotency determined and maintained?
... this particular complex would normally prevent ES cells from differentiating into primitive endoderm by repressing the trigger factor, Gata6. Nanog is an NK-2 class homeobox transcription factor that is expressed throughout the pluripotent cells of the ICM. As overexpression of Nanog in mouse ES cel ...
... this particular complex would normally prevent ES cells from differentiating into primitive endoderm by repressing the trigger factor, Gata6. Nanog is an NK-2 class homeobox transcription factor that is expressed throughout the pluripotent cells of the ICM. As overexpression of Nanog in mouse ES cel ...
The Cellular Mechanism of Epithelial Rearrangement during
... Dorsal intercalation can also be analyzed in living embryos using Nomarski microscopy. In hypodermal tissue visualized by Nomarski microscopy, cellular boundaries appear as depressions in the hypodermal sheet, and nuclei can be visualized as exclusions of cytoplasmic granules (Fig. 2). Playback of t ...
... Dorsal intercalation can also be analyzed in living embryos using Nomarski microscopy. In hypodermal tissue visualized by Nomarski microscopy, cellular boundaries appear as depressions in the hypodermal sheet, and nuclei can be visualized as exclusions of cytoplasmic granules (Fig. 2). Playback of t ...
Lonza Group Ltd
... completed the acquisition of the preclinical cell and molecular biology assets of Simbiosys Biowares India Pvt. Ltd as of 13 October 2009. Simbiosys Biowares is a premier biology outsourcing company with core competencies in cell and molecular biology, and in clinical services. The company’s cell an ...
... completed the acquisition of the preclinical cell and molecular biology assets of Simbiosys Biowares India Pvt. Ltd as of 13 October 2009. Simbiosys Biowares is a premier biology outsourcing company with core competencies in cell and molecular biology, and in clinical services. The company’s cell an ...
Genomic Tagging of the Anaphase-Promoting Complex Activator
... molecules can have an important role in signaling and post-translational modifications of some proteins; however, addition of four or more units is termed polyubiquitylation and targets the protein for degradation by the 26S proteasome (16). This is exactly the pathway via which APC/C fulfills its m ...
... molecules can have an important role in signaling and post-translational modifications of some proteins; however, addition of four or more units is termed polyubiquitylation and targets the protein for degradation by the 26S proteasome (16). This is exactly the pathway via which APC/C fulfills its m ...
Mechanism of polarized lysosome exocytosis in epithelial cells
... Lysosomal membrane proteins LAMP1 and LAMP2 are transported from the trans-Golgi network to lysosomes via clathrin-coated transport intermediates. LAMPs contain Cterminal tyrosine-based sorting signals (YXXW), which bind the medium (m) subunits of clathrin-adaptors AP-1, AP-2, AP-3 and AP-4 (Ohno et ...
... Lysosomal membrane proteins LAMP1 and LAMP2 are transported from the trans-Golgi network to lysosomes via clathrin-coated transport intermediates. LAMPs contain Cterminal tyrosine-based sorting signals (YXXW), which bind the medium (m) subunits of clathrin-adaptors AP-1, AP-2, AP-3 and AP-4 (Ohno et ...
On the so-called "Sexual" Method of Spore
... For various reasons, it seemed to me that the process which Schaudinn (1902) and others—including myself (1908)—were driven to regard as a form of conjugation, was, perhaps, capable of being interpreted in some other way. After much idle guessing, I concluded that the only method of throwing light u ...
... For various reasons, it seemed to me that the process which Schaudinn (1902) and others—including myself (1908)—were driven to regard as a form of conjugation, was, perhaps, capable of being interpreted in some other way. After much idle guessing, I concluded that the only method of throwing light u ...
Influence of Antibiotic and E5 Monoclonal Immunoglobulin
... The effect of DODAB concentration (C) on cell viability at a fixed bacteria concentration (ca. 2.5 x 107 bacteria/mL) and interaction time (1 h) for 4 different model microorganisms is shown in Figure 1. As cell concentration is the same for the 4 species, all other experimental conditions also bein ...
... The effect of DODAB concentration (C) on cell viability at a fixed bacteria concentration (ca. 2.5 x 107 bacteria/mL) and interaction time (1 h) for 4 different model microorganisms is shown in Figure 1. As cell concentration is the same for the 4 species, all other experimental conditions also bein ...
Cell surface monoamine oxidases: enzymes in search of a function
... transient covalent enzyme±substrate intermediates, this reaction results in production of aldehydes, hydrogen peroxide and ammonium, which are all biologically active substances. The physiological functions of these enzymes have remained unknown, although they have been suggested to be involved in t ...
... transient covalent enzyme±substrate intermediates, this reaction results in production of aldehydes, hydrogen peroxide and ammonium, which are all biologically active substances. The physiological functions of these enzymes have remained unknown, although they have been suggested to be involved in t ...
Leaf growth in grasses is determined by the cell division and
... and final cell length. However, the response of the leaf expansion rate to salinity was analysed mainly in terms of changes in cell turgor and cell wall properties. Because the time required for a cell to leave the growth zone was higher under control conditions than under salinity, cell flux in the ...
... and final cell length. However, the response of the leaf expansion rate to salinity was analysed mainly in terms of changes in cell turgor and cell wall properties. Because the time required for a cell to leave the growth zone was higher under control conditions than under salinity, cell flux in the ...
Degradation of plant cell wall polymers
... bioremediation programmes and characterization of its ligninases is now well understood, providing information that can be related to its ecophysiology in the natural environment. A good example is the regulatory effect of nitrogen on ligninolytic enzyme expression, a reflection of the inductive effect ...
... bioremediation programmes and characterization of its ligninases is now well understood, providing information that can be related to its ecophysiology in the natural environment. A good example is the regulatory effect of nitrogen on ligninolytic enzyme expression, a reflection of the inductive effect ...
differentiation and proliferation of embryonic mast
... stages were identified. Stage I mast cells are lymphocyte-like cells with cytoplasmic granules which invariably stain blue with the alcian blue-safranin reaction. In Stage I I cells the majority of granules are alcian blue-positive, but some safranin-positive granules have appeared. Stage I I I mast ...
... stages were identified. Stage I mast cells are lymphocyte-like cells with cytoplasmic granules which invariably stain blue with the alcian blue-safranin reaction. In Stage I I cells the majority of granules are alcian blue-positive, but some safranin-positive granules have appeared. Stage I I I mast ...
Recent Progress in Research of the Mechanism of Fertilization in
... fusing with the egg cell and central cell. Both male and female gametes belong to the G2 Type. However, Friedman could not measure egg cell DNA changes because the nuclei of mature egg cells have no fluorescence (Friedman, pers. comm.). These results lead us to question how the DNA content of eggs a ...
... fusing with the egg cell and central cell. Both male and female gametes belong to the G2 Type. However, Friedman could not measure egg cell DNA changes because the nuclei of mature egg cells have no fluorescence (Friedman, pers. comm.). These results lead us to question how the DNA content of eggs a ...
Cell Wall Amine Oxidases: New Players in Root Xylem
... mainly depend on events of PA secretion, which may occur under environmental stresses or at specific developmental stages. Indeed, while in the absence of any internal or external stimulus, PAs are present in the cell wall at very low or even undetectable levels [51], under specific adverse conditio ...
... mainly depend on events of PA secretion, which may occur under environmental stresses or at specific developmental stages. Indeed, while in the absence of any internal or external stimulus, PAs are present in the cell wall at very low or even undetectable levels [51], under specific adverse conditio ...
NIH Public Access
... The decision for progenitors to differentiate is made at the G1 phase of the cell cycle where Dtype cyclins and cyclin-dependent kinases inhibitors (CKIs) play an important role in regulating cell cycle exit. The CKIs regulate cell cycle progression by blocking phosphorylation of the retinoblastoma ...
... The decision for progenitors to differentiate is made at the G1 phase of the cell cycle where Dtype cyclins and cyclin-dependent kinases inhibitors (CKIs) play an important role in regulating cell cycle exit. The CKIs regulate cell cycle progression by blocking phosphorylation of the retinoblastoma ...
Dissociation of Centrosome Replication Events from Cycles of DNA
... a seeding capacity of some form that is required for centriole replication to occur. Still, it is not clear how components of the pericentriolar material are replicated and assembled, nor is it understood how the cell is able to regulate the timing of centrosome duplication each cell cycle. As state ...
... a seeding capacity of some form that is required for centriole replication to occur. Still, it is not clear how components of the pericentriolar material are replicated and assembled, nor is it understood how the cell is able to regulate the timing of centrosome duplication each cell cycle. As state ...
Centromeres: An Integrated Protein/DNA Complex
... Ill is not known,but suggestsa role for structural DNA in this unique protein/DNAcomplex. CentromereDNAfrom a related species of Saccharomyes,S. uvarum, can stabilize minichromosomes in S. cerevisiae, and it contains the conserved sequenceelementsCDEI-III. It is unlikely that centromerefunction exte ...
... Ill is not known,but suggestsa role for structural DNA in this unique protein/DNAcomplex. CentromereDNAfrom a related species of Saccharomyes,S. uvarum, can stabilize minichromosomes in S. cerevisiae, and it contains the conserved sequenceelementsCDEI-III. It is unlikely that centromerefunction exte ...
Quality control in the initiation of eukaryotic DNA replication
... to G1 phase, when origins cannot fire because CDKs are absent, and activation of CDK at the end of G1 phase triggers initiation from licensed origins, and prevents the re-assembly of pre-RCs at origins that have fired. The role for CDK in preventing licensing outside of G1 phase has been conserved i ...
... to G1 phase, when origins cannot fire because CDKs are absent, and activation of CDK at the end of G1 phase triggers initiation from licensed origins, and prevents the re-assembly of pre-RCs at origins that have fired. The role for CDK in preventing licensing outside of G1 phase has been conserved i ...
Bacterial ancestry of actin and tubulin Fusinita van den Ent, Linda
... In eukaryotic cells, motor proteins that operate on the cytoskeletal network facilitate active transport of cargo [46]. The small size and apparent lack of compartments in bacterial cells enables free diffusion of proteins in milliseconds [47]. However, it has been shown that many proteins as well a ...
... In eukaryotic cells, motor proteins that operate on the cytoskeletal network facilitate active transport of cargo [46]. The small size and apparent lack of compartments in bacterial cells enables free diffusion of proteins in milliseconds [47]. However, it has been shown that many proteins as well a ...