Dissecting stimulus-specific Ca2+ signals in amyloplasts and
... signal transduction. This allowed us to monitor in parallel Ca2+ dynamics in the bulk cytosol, the cytosolic microdomain just outside plastids (by means of the aequorin chimera targeted to the cytosolic surface of the plastid outer envelope), and the organelle stroma. [Ca2+] in all these subcellular ...
... signal transduction. This allowed us to monitor in parallel Ca2+ dynamics in the bulk cytosol, the cytosolic microdomain just outside plastids (by means of the aequorin chimera targeted to the cytosolic surface of the plastid outer envelope), and the organelle stroma. [Ca2+] in all these subcellular ...
Live cell imaging reveals actin-cytoskeleton
... Crosslinking of actin filaments into bundles is essential for the assembly and stabilization of specific cytoskeletal structures. However, relatively little is known about the molecular mechanisms underlying actin bundle formation. The two LIMdomain-containing proteins define a novel and evolutionar ...
... Crosslinking of actin filaments into bundles is essential for the assembly and stabilization of specific cytoskeletal structures. However, relatively little is known about the molecular mechanisms underlying actin bundle formation. The two LIMdomain-containing proteins define a novel and evolutionar ...
MAPK-mediated Phosphorylation of GATA-1 Promotes Expression and Cell Survival* Bcl-X □
... motifs in the 5⬘ promoter region (7), and ectopic expression of GATA-1 induces the expression of Bcl-XL in GATA-1⫺/⫺ erythroid progenitor cells (8). GATA-1 also suppresses IL1-6-induced apoptosis and up-regulates the expression of Bcl-2 in M1 myeloid cells (9). Moreover, GATA elements are found in t ...
... motifs in the 5⬘ promoter region (7), and ectopic expression of GATA-1 induces the expression of Bcl-XL in GATA-1⫺/⫺ erythroid progenitor cells (8). GATA-1 also suppresses IL1-6-induced apoptosis and up-regulates the expression of Bcl-2 in M1 myeloid cells (9). Moreover, GATA elements are found in t ...
Cell regulation by the Apc protein Apc as master regulator of epithelia
... The first recognised function of Apc was its role in Wnt signalling [4,5]. This function is one of the driving forces for how mutations in Apc ensure that cells remain proliferative. Many of the molecular details of this pathway have been described extensively in many reviews [6]. Apc negatively ...
... The first recognised function of Apc was its role in Wnt signalling [4,5]. This function is one of the driving forces for how mutations in Apc ensure that cells remain proliferative. Many of the molecular details of this pathway have been described extensively in many reviews [6]. Apc negatively ...
A microfluidic culture platform for CNS axonal injury, regeneration
... to speculate that the existence of axonal pools of synaptophysin mRNA, which we detected using RT-PCR, may lead to local protein synthesis of this synaptic vesicle protein and thereby mediate this neurotransmitter release. Spinal cord injury is a challenging field of study, in part because simple an ...
... to speculate that the existence of axonal pools of synaptophysin mRNA, which we detected using RT-PCR, may lead to local protein synthesis of this synaptic vesicle protein and thereby mediate this neurotransmitter release. Spinal cord injury is a challenging field of study, in part because simple an ...
FGF signalling: diverse roles during early vertebrate embryogenesis
... FGF signalling is required for the formation of axial and paraxial mesoderm, the two mesodermal cell types that are primarily responsible for orchestrating the movements of gastrulation, it could not easily be discerned whether the defect in cell movements was a direct or indirect effect, mediated b ...
... FGF signalling is required for the formation of axial and paraxial mesoderm, the two mesodermal cell types that are primarily responsible for orchestrating the movements of gastrulation, it could not easily be discerned whether the defect in cell movements was a direct or indirect effect, mediated b ...
J., Schornack, S., Spallek, T., Geldner, N., Chory
... (Fig. S1A). The cytoplasm of infected cells was aggregated at the haustorial site as detected in 35S::GFP expressing transgenic lines, and the nucleus labelled by Hoechst staining was repeatedly found in close proximity to the Hpa haustorium, as recently described (Caillaud et al., 2011). Evidently, ...
... (Fig. S1A). The cytoplasm of infected cells was aggregated at the haustorial site as detected in 35S::GFP expressing transgenic lines, and the nucleus labelled by Hoechst staining was repeatedly found in close proximity to the Hpa haustorium, as recently described (Caillaud et al., 2011). Evidently, ...
Notochord vacuoles are lysosome-related organelles that function in
... et al., 1996; Stemple et al., 1996; Coutinho et al., 2004; Melville et al., 2011). Next, to investigate the potential role of post-Golgi trafficking in vacuole formation we asked whether clathrin coats are required. Clathrin coats are important mediators of coated vesicle formation in post-Golgi tra ...
... et al., 1996; Stemple et al., 1996; Coutinho et al., 2004; Melville et al., 2011). Next, to investigate the potential role of post-Golgi trafficking in vacuole formation we asked whether clathrin coats are required. Clathrin coats are important mediators of coated vesicle formation in post-Golgi tra ...
Through form to function: root hair development and nutrient uptake
... specification, initiation, subsequent tip growth and maturation (Fig. 1). Because of these distinct stages of development, root hairs have been used as a model system to begin to understand how plants: • Specify cell fate (whether a cell is destined to make a root hair or not). • Encode positional i ...
... specification, initiation, subsequent tip growth and maturation (Fig. 1). Because of these distinct stages of development, root hairs have been used as a model system to begin to understand how plants: • Specify cell fate (whether a cell is destined to make a root hair or not). • Encode positional i ...
here - The Nebenführ Lab
... apparatus. Although this toxin has been used in many studies, its effects on plant cells are still shrouded in controversy. We have reinvestigated the early responses of plant cells to BFA with novel tools, namely, tobacco Bright Yellow 2 (BY-2) suspension-cultured cells expressing an in vivo green ...
... apparatus. Although this toxin has been used in many studies, its effects on plant cells are still shrouded in controversy. We have reinvestigated the early responses of plant cells to BFA with novel tools, namely, tobacco Bright Yellow 2 (BY-2) suspension-cultured cells expressing an in vivo green ...
Cleavage modification did not alter early blastomere fates
... bioRxiv preprint first posted online Aug. 11, 2016; doi: http://dx.doi.org/10.1101/068783. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
... bioRxiv preprint first posted online Aug. 11, 2016; doi: http://dx.doi.org/10.1101/068783. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It is made available under a CC-BY 4.0 International license. ...
Structural Sterols Are Involved in Both the Initiation and Tip Growth
... in plants, and it is present only in certain tissues during discrete developmental stages (Hobbs et al., 1996; Arnqvist et al., 2003). In general, the production rate of sterols varies in different cell types. Thus, it is high in proliferating and actively growing tissues, while it declines in matur ...
... in plants, and it is present only in certain tissues during discrete developmental stages (Hobbs et al., 1996; Arnqvist et al., 2003). In general, the production rate of sterols varies in different cell types. Thus, it is high in proliferating and actively growing tissues, while it declines in matur ...
Linker histone H1 in early mouse embryogenesis
... By immunostaining (Fig. 1B, anti-H1P panel), this antibody recognized a nuclear protein in MEL cells and in mouse blastula cells, with a variable staining level in the nucleus during interphase, and a much more intense staining on chromatin during mitosis. At this latter stage, a higher cytoplasmic ...
... By immunostaining (Fig. 1B, anti-H1P panel), this antibody recognized a nuclear protein in MEL cells and in mouse blastula cells, with a variable staining level in the nucleus during interphase, and a much more intense staining on chromatin during mitosis. At this latter stage, a higher cytoplasmic ...
The Pathogen-Actin Connection: A Platform for Defense Signaling in
... however, a number of studies have independently investigated functional and regulatory processes that potentially link MAPK signaling and cortical actin dynamics (44, 104, 105, 107). Signaling cascades are often downregulated by removing the receptor and its ligand from the cell surface via receptor ...
... however, a number of studies have independently investigated functional and regulatory processes that potentially link MAPK signaling and cortical actin dynamics (44, 104, 105, 107). Signaling cascades are often downregulated by removing the receptor and its ligand from the cell surface via receptor ...
1. The BCL-2 Family Reunion.
... are the major members of the antiapoptotic BCL-2 repertoire and preserve OMM integrity by directly inhibiting the proapoptotic BCL-2 proteins. The proapoptotic BCL-2 members are divided into the effector proteins and the BH3-only proteins. The effector proteins BCL-2 antagonist killer 1 (BAK) and BC ...
... are the major members of the antiapoptotic BCL-2 repertoire and preserve OMM integrity by directly inhibiting the proapoptotic BCL-2 proteins. The proapoptotic BCL-2 members are divided into the effector proteins and the BH3-only proteins. The effector proteins BCL-2 antagonist killer 1 (BAK) and BC ...
Pericentriolar material structure and dynamics
... indicating that they are similar, but not identical, to normal mitotic centrosomes [7,70]. These results hint that the core PCM proteins can self-assemble without external stimulation by mitotic kinases like polo. Under physiological conditions, the effective concentration of the core PCM proteins m ...
... indicating that they are similar, but not identical, to normal mitotic centrosomes [7,70]. These results hint that the core PCM proteins can self-assemble without external stimulation by mitotic kinases like polo. Under physiological conditions, the effective concentration of the core PCM proteins m ...
Pollen Tube Growth Oscillations and Intracellular
... When 2 nM Lat-B is administered to pollen tubes exhibiting oscillatory growth, there is no apparent effect for the first 5 min (Fig. 1A). However, during the next 10 to 15 min the overall growth rate starts to decline; the oscillations become erratic with a marked diminution in excursions from the m ...
... When 2 nM Lat-B is administered to pollen tubes exhibiting oscillatory growth, there is no apparent effect for the first 5 min (Fig. 1A). However, during the next 10 to 15 min the overall growth rate starts to decline; the oscillations become erratic with a marked diminution in excursions from the m ...
The Drosophila ribbon gene encodes a nuclear BTB domain protein that promotes epithelial migration and morphogenesis. Development 128, 4923-4933. pdf
... tracheal branching (Manning and Krasnow, 1993; Metzger and Krasnow, 1999; Affolter and Shilo, 2000). These have begun to separate the morphogenetic process into genetically distinct steps. Several genes are required for the initial specification of tracheal cell fate and invagination to form trachea ...
... tracheal branching (Manning and Krasnow, 1993; Metzger and Krasnow, 1999; Affolter and Shilo, 2000). These have begun to separate the morphogenetic process into genetically distinct steps. Several genes are required for the initial specification of tracheal cell fate and invagination to form trachea ...
Formation of Persisting Cell Wall Deficient Forms of Mycobacterium
... phagocytosis by peritoneal macrophages in guinea pigs. Although peritoneal macrophages are not typical niche for mycobacteria, we attempted to inhibit the classical bacterial forms and to induce occurrence of cell wall deficient forms by using an unusual unfavorable location (peritoneal cavity) of i ...
... phagocytosis by peritoneal macrophages in guinea pigs. Although peritoneal macrophages are not typical niche for mycobacteria, we attempted to inhibit the classical bacterial forms and to induce occurrence of cell wall deficient forms by using an unusual unfavorable location (peritoneal cavity) of i ...