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Stem cells: the intestinal stem cell as a paradigm
Stem cells: the intestinal stem cell as a paradigm

... to treating such uncontrolled proliferation, providing us with targets at which future cancer treatments including gene therapy can be aimed. (ii) The rapidly dividing tissues of bone marrow, gut and skin are the first to be affected by cancer treatment. Toxicity in these tissues is dose limiting fo ...
Lecture 15 - People Server at UNCW
Lecture 15 - People Server at UNCW

... ClHCO3osmotically ...
Differential Expression of Four Connexin Genes, Cx-26, Cx
Differential Expression of Four Connexin Genes, Cx-26, Cx

Breaking Down the Complex Regulatory Web
Breaking Down the Complex Regulatory Web

... cells using microarray analysis. Protein sequence alignment and phylogenetic analysis were used to identify candidate genes, which were tested using RNA silencing, culminating in the identification of candidate genes encoding enzymes involved in the early steps of the currently accepted monolignol b ...
Answers to Mastering Concepts Questions
Answers to Mastering Concepts Questions

... In theory, genes encoding enzymes required for nitrogen fixation could be moved from nitrogen-fixing bacteria and inserted into the chromosomes of a plant. It would be potentially valuable to agriculture because plants could fix their own atmospheric nitrogen, which would reduce the need for nitroge ...
The Cell Membrane
The Cell Membrane

...  Play a key role in cell-cell recognition ...
Chapter 7: Life is Cellular
Chapter 7: Life is Cellular

... molecules across the membrane. This process is called facilitated diffusion. It does not require use of the cell’s energy. Active transport does require the cell’s energy. Active transport is when cells move materials from one side of a membrane to the other side against the concentration difference ...
Global impact of Salmonella type III secretion effector SteA on host
Global impact of Salmonella type III secretion effector SteA on host

... adhesion to plastic substrate or extracellular matrix proteins (collagen I or vitronectin) was not altered in these cells (data not shown), cell-cell adhesion was clearly disrupted. To carry out the later experiments, cells were cultured in poly-HEMA treated plates. In this way, cells are not able t ...
Fulltext PDF - Indian Academy of Sciences
Fulltext PDF - Indian Academy of Sciences

Cell Cycle & Mitosis
Cell Cycle & Mitosis

... mitosis at all because bacteria have only one chromosome attached to a cell membrane and use fission to create more bacteria. ...
Molecular anatomy of antigen-specific CD8+ T cell
Molecular anatomy of antigen-specific CD8+ T cell

... Because TCR-Tg mice were used to visualize in vivo immunologic synapse formation, it is possible that the TCR focusing was not representative of natural effector–target cell interactions. Thus, we next wanted to validate our findings in nontransgenic B6 mice. B6 mice infected intracerebrally with LC ...
Development of genital system
Development of genital system

... • In the meantime, another pair of elevation, genital swelling become visible on each side of the urethral folds • These will form labia majora • Estrogens stimulate development of the external genitalia of the female • The genital tubercle elongates slightly to ...
Standard PDF - Wiley Online Library
Standard PDF - Wiley Online Library

... Figure 2. Possible molecular mechanisms for mechanotransduction at the AJ, focusing on a-catenin-actin interaction. A: The structure and functional domains of a-catenin, showing bcatenin, vinculin, and F-actin (actin filament) binding regions and an inhibitory region for vinculin binding. The vincul ...
Alternative translation initiation gives rise to two isoforms of Orai1
Alternative translation initiation gives rise to two isoforms of Orai1

... same size as WT Orai1b (Fig. 2A). This result strongly suggested that the shorter isoform is translated from an alternative translation start site. Orai1 has two methionine residues downstream of the first in its N-terminus, amino acids 64 and 71. Next, we mutated these methionines to alanine or val ...
Get PDF file - Botanik in Bonn
Get PDF file - Botanik in Bonn

... man Zelle und Zellinhalt.’ ‘…the science which deals with living matter commenced with a controversial word, originating as a mistake more than 200 years ago, and then maintained up to the present day: it is the word Cell.’ ‘It is well-known that the word Cell should be understood only from a histor ...
Travel Brochure of a Cell
Travel Brochure of a Cell

... Travel Brochure of a Cell Create a travel brochure that describes an animal or plant cell as if it were a museum or amusement park. Your brochure must attract visitors to spend money to visit a plant or animal cell. You can think of it as a huge amusement park or a small roadside attraction. Your at ...
Roseoloviruses manipulate host cell cycle - HHV
Roseoloviruses manipulate host cell cycle - HHV

... for HSV-1 replication [7]. Thus, the alphaherpesviruses may be less dependent on cellular E2F-responsive genes for viral DNA replication than other herpesviruses and may not need to target Rb family members for inactivation. The gammaherpesviruses are associated with proliferative disorders includin ...
THE USE OF PHOSPHORUS 32 IN STUDIES ON PLASMODIUM
THE USE OF PHOSPHORUS 32 IN STUDIES ON PLASMODIUM

... was adjusted to give a final cation molarity of approximately 0.15 in the diluent with NaCI at 0.13 xt, KC1 at 0.004 M, and MgSO4 at 0.001 x¢. In the earlier experiments, CaC12 was included at 0.0005 M, but this salt was later omitted without apparent effect on parasite growth. Although it was desir ...
But what about bacterial cells
But what about bacterial cells

... Well, they can always move with the help of their flagella to where the food is. But some bacteria do not have flagella! These bacteria must be able to get the nutrients it needs in order to survive. One way that the bacteria can do this is to make its own food...just like a plant cell! ...
Cell cycle arrest and apoptosis induced by the coronavirus
Cell cycle arrest and apoptosis induced by the coronavirus

PilB localization determines the direction of twitching
PilB localization determines the direction of twitching

... because of loss of T4P function. Therefore wild-type cells were first transformed with ...
PDF
PDF

... and elevated expression of Cas9 in target cells) that could lead to off­target effects. To address these challenges, we have developed a system of cell­derived nanovesicles called gesicles. Gesicles contain active Cas9 protein complexed with an sgRNA specific to a gene of interest. Thus, there is no ...
Evolution of Cell Division
Evolution of Cell Division

... and cell division [18, 19, 20]. However, different cell types attain this coordination through different mechanisms. Three major mechanisms have been proposed for this coordination [18]. In some cells, cell division is dependant on cell growth [13, 18, 19, 20]. This means that the cell cycle would o ...
SICKLE CELL ANEMIA
SICKLE CELL ANEMIA

... • Glutamate  Valine at 6th position b globin • Sickle Hb forms polymers when deoxygenated • Polymerized sickle Hb injures RBC membrane and distorts its shape • Distorted RBC is hemolyzed ...
c-IAP1 shuttling from the nucleus to the Golgi apparatus in cells
c-IAP1 shuttling from the nucleus to the Golgi apparatus in cells

... cellular inhibitors of cell death. These proteins were identified in baculoviral genome as regulators of host-cell viability during virus infection1 and cellular orthologues were subsequently described in yeast, nematodes, drosophila and mammals. The human genome encodes at least eight IAPs (XIAP, c ...
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Cell culture



Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.
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