Degradable polymers for tissue engineering
... difficult task and therefore various materials have been explored to provide substrates for cell growth in vivo . The physical and chemical properties of these materials are important. These include natural polymers, degradable polymers, and bioceramics. ...
... difficult task and therefore various materials have been explored to provide substrates for cell growth in vivo . The physical and chemical properties of these materials are important. These include natural polymers, degradable polymers, and bioceramics. ...
regulation of cell growth by vitreous humour
... heated vitreous/ml of medium. In this experiment only 16 000 cells adhered per well in the 4-h attachment period after plating. The effects of heating were investigated because of evidence for heat-labile mitogens in bovine vitreous (Raymond & Jacobson, 1982). The arrest in proliferation can be cont ...
... heated vitreous/ml of medium. In this experiment only 16 000 cells adhered per well in the 4-h attachment period after plating. The effects of heating were investigated because of evidence for heat-labile mitogens in bovine vitreous (Raymond & Jacobson, 1982). The arrest in proliferation can be cont ...
B cells in Type 1 diabetes: Studies on cell surface antibody binding
... mainly gathered in the cortex in areas called primary follicles. Here they are in close contact with follicular dendritic cells which can carry antigens on their surface, bound to IgM, IgG or complement. These antigens can stimulate B cells through BCR crosslinking, expression of other interacting s ...
... mainly gathered in the cortex in areas called primary follicles. Here they are in close contact with follicular dendritic cells which can carry antigens on their surface, bound to IgM, IgG or complement. These antigens can stimulate B cells through BCR crosslinking, expression of other interacting s ...
word - My eCoach
... The cell cycle includes all of the phasesin the life of a cell. The cell cycle is a repeating sequenceof cellular growth and division during the life of an organism. Mitosis is one of the phasesin the cell cycle. Mitosis is the process by which the material in a cell's nucleus is divided during cell ...
... The cell cycle includes all of the phasesin the life of a cell. The cell cycle is a repeating sequenceof cellular growth and division during the life of an organism. Mitosis is one of the phasesin the cell cycle. Mitosis is the process by which the material in a cell's nucleus is divided during cell ...
Weak pairwise correlations imply strongly correlated network states in a neural population
... 40 retinal ganglion cells as they respond to movies taken from a natural setting (see Methods). The correlations between cells have structure on the scale of 20 ms and we use this window as our typical Dt. The small values of the correlation coefficients suggest an approximation in which the cells a ...
... 40 retinal ganglion cells as they respond to movies taken from a natural setting (see Methods). The correlations between cells have structure on the scale of 20 ms and we use this window as our typical Dt. The small values of the correlation coefficients suggest an approximation in which the cells a ...
Cellular Structure and Function
... The answer to these questions is clear once you know how a cell functions. To carry out life processes, a cell must be able to quickly pass substances into and out of the cell. For example, it must be able to pass nutrients and oxygen into the cell and waste products out of the cell. Anything that e ...
... The answer to these questions is clear once you know how a cell functions. To carry out life processes, a cell must be able to quickly pass substances into and out of the cell. For example, it must be able to pass nutrients and oxygen into the cell and waste products out of the cell. Anything that e ...
Recombination Mediators across Cell Cycle Stage by Regulating
... blocking solution for 1 h at room temperature. Cells were rinsed three times in 0.2% BSA and 0.1% Tween 20/PBS and incubated for 1 h with goat-anti-rabbit IgG Alexa 488 or 594 or 633 (Invitrogen). After three rinses in 0.1% Tween 20/PBS, cells were postfixed in 3% paraformaldehyde/PBS for 10 min, pe ...
... blocking solution for 1 h at room temperature. Cells were rinsed three times in 0.2% BSA and 0.1% Tween 20/PBS and incubated for 1 h with goat-anti-rabbit IgG Alexa 488 or 594 or 633 (Invitrogen). After three rinses in 0.1% Tween 20/PBS, cells were postfixed in 3% paraformaldehyde/PBS for 10 min, pe ...
Honey bees are predisposed to win-shift but can learn to win-stay
... often than expected by chance or less often than expected by chance. The ability of bees in nature to choose flowers contingent on the identity of recently visited flowers would have obvious adaptive value. We (Brown & Demas 1994) have shown that memory for previous visits allows previously visited ...
... often than expected by chance or less often than expected by chance. The ability of bees in nature to choose flowers contingent on the identity of recently visited flowers would have obvious adaptive value. We (Brown & Demas 1994) have shown that memory for previous visits allows previously visited ...
Cell-Cell Interactions
... of chemical signals, they coordinate their behavior so that your body functions as an integrated whole, rather than as a massive collection of individual cells acting independently. The ability of cells to communicate with one another is the hallmark of multicellular organisms. In this chapter we wi ...
... of chemical signals, they coordinate their behavior so that your body functions as an integrated whole, rather than as a massive collection of individual cells acting independently. The ability of cells to communicate with one another is the hallmark of multicellular organisms. In this chapter we wi ...
Munc13-4 rab27 complex is specifically required for
... Interaction assays with the point mutants, showed that munc13-4(FQL>AAA) and munc134(IHK>AAA) mutants lost 80% rab27a binding, while a gapped construct munc13-4Δ(280285) lacking the FQLIHK residues, confirmed their requirement (Fig. 1F). The sequence of this region is only modestly conserved among m ...
... Interaction assays with the point mutants, showed that munc13-4(FQL>AAA) and munc134(IHK>AAA) mutants lost 80% rab27a binding, while a gapped construct munc13-4Δ(280285) lacking the FQLIHK residues, confirmed their requirement (Fig. 1F). The sequence of this region is only modestly conserved among m ...
ACP Level 1 A Microscopic Look at Essential Oils
... Plant chemistry became an established university discipline at the end of the 19th century; since then many new structures have been discovered. The number of natural products obtained from plants exceeds 100,000 and each year new chemical compounds are discovered. Although the functions of some pla ...
... Plant chemistry became an established university discipline at the end of the 19th century; since then many new structures have been discovered. The number of natural products obtained from plants exceeds 100,000 and each year new chemical compounds are discovered. Although the functions of some pla ...
CycD1, a Putative G1 Cyclin from Antirrhinum majus
... CycD1 Is Located in the Interphase Nucleus The location and timing of a cyclin’s appearance can provide clues as to its probable function (Draviam et al., 2001; Jackman et al., 2003). Therefore, as a first step, we transiently expressed green fluorescent protein (GFP):CycD1 under the 35S promoter of ...
... CycD1 Is Located in the Interphase Nucleus The location and timing of a cyclin’s appearance can provide clues as to its probable function (Draviam et al., 2001; Jackman et al., 2003). Therefore, as a first step, we transiently expressed green fluorescent protein (GFP):CycD1 under the 35S promoter of ...
Embryonic stem cell differentiation and the analysis of mammalian
... populations and no evidence was found for spatial heterogeneity within this pool. Molecular homogeneity of pluripotent cells in the pre-gastrulation embryo is consistent with the fate of heterotopic transplanted primitive ectoderm from pre-streak or early primitive streak embryos, which assumes a fa ...
... populations and no evidence was found for spatial heterogeneity within this pool. Molecular homogeneity of pluripotent cells in the pre-gastrulation embryo is consistent with the fate of heterotopic transplanted primitive ectoderm from pre-streak or early primitive streak embryos, which assumes a fa ...
WWW.BROOKES.AC.UK/GO/RADAR
... cells of Arabidopsis a precise, observation-based statement that contradicts the common text book knowledge has been made by Pyke (2009): “In a leaf, the chloroplasts in the epidermal cells covering the leaf surface are significantly smaller and poorly developed compared with mesophyll chloroplasts, ...
... cells of Arabidopsis a precise, observation-based statement that contradicts the common text book knowledge has been made by Pyke (2009): “In a leaf, the chloroplasts in the epidermal cells covering the leaf surface are significantly smaller and poorly developed compared with mesophyll chloroplasts, ...
Measuring Mitochondrial Membrane Potential using
... mitochondria to generate ATP and provide energy for other cellular components.3 The number of factors which can influence this potential are vast and include many cellular components outside the mitochondria, for example interactions with the activity of the proteasome. In addition, the ability of a ...
... mitochondria to generate ATP and provide energy for other cellular components.3 The number of factors which can influence this potential are vast and include many cellular components outside the mitochondria, for example interactions with the activity of the proteasome. In addition, the ability of a ...
Infrared spectroscopic study of bryostatin 1
... The WSU-CLL line was established at Wayne State University from a 66-year-old male with CLL refractory to CVP (cyclophosphamide, vincristine and prednisone), fludarabine and VAD (vincristine, adriamycin and dexamethasone).13 These cells grow as a suspension in liquid culture and form subcutaneous tu ...
... The WSU-CLL line was established at Wayne State University from a 66-year-old male with CLL refractory to CVP (cyclophosphamide, vincristine and prednisone), fludarabine and VAD (vincristine, adriamycin and dexamethasone).13 These cells grow as a suspension in liquid culture and form subcutaneous tu ...
Foreword
... With a limited understanding of the “critical “ components of cell structure, their interactions and roles within the cell and the genes that control them we are constrained in our ability to improve these elements through breeding or other approaches such as genetic modification. This lack of knowl ...
... With a limited understanding of the “critical “ components of cell structure, their interactions and roles within the cell and the genes that control them we are constrained in our ability to improve these elements through breeding or other approaches such as genetic modification. This lack of knowl ...
Histology Webquest
... This will begin to prepare you for the next unit-Histology. This is to be completed during class and anything that you do not finish is homework and due at the beginning of the next class. Go to: http://training.seer.cancer.gov/anatomy/cells_tissues_membranes/tissues/ 1. Answer the following questio ...
... This will begin to prepare you for the next unit-Histology. This is to be completed during class and anything that you do not finish is homework and due at the beginning of the next class. Go to: http://training.seer.cancer.gov/anatomy/cells_tissues_membranes/tissues/ 1. Answer the following questio ...
Cell cycle`s deregulation and cardiovascular diseases
... • AIF-1 is not present in normal arteries, and it is produced by VSMC when they are stimulated. Constitutive expression of AIF-1 results in a shorter cell cycle, and aberrant expression of cell cycle proteins. • AIF-1 actively participates in the upregulation of VSMC’s proliferation ...
... • AIF-1 is not present in normal arteries, and it is produced by VSMC when they are stimulated. Constitutive expression of AIF-1 results in a shorter cell cycle, and aberrant expression of cell cycle proteins. • AIF-1 actively participates in the upregulation of VSMC’s proliferation ...
Long-term live imaging provides new insight into stem cell
... the basement membrane (Fig. 1C⬘, blue). Follicle cells surround more mature germline cysts and display distinctive oblong nuclei (Fig. 1C⬘, green). The positions of specific cells were then tracked manually, frame by frame, throughout entire movies using Imaris software (Fig. 1D,D⬘; see Materials an ...
... the basement membrane (Fig. 1C⬘, blue). Follicle cells surround more mature germline cysts and display distinctive oblong nuclei (Fig. 1C⬘, green). The positions of specific cells were then tracked manually, frame by frame, throughout entire movies using Imaris software (Fig. 1D,D⬘; see Materials an ...
RNA analysis by RT-PCR and restriction digestion
... 2-mercaptoethanol (2-ME) (69). Despite controversies over the specificity of this procedure it remains the only method of determining virus neutralizing IgG reliably in a plaque reduction assay because specific secondary antibodies cannot be used. Total neutralizing serum antibody concentrations exc ...
... 2-mercaptoethanol (2-ME) (69). Despite controversies over the specificity of this procedure it remains the only method of determining virus neutralizing IgG reliably in a plaque reduction assay because specific secondary antibodies cannot be used. Total neutralizing serum antibody concentrations exc ...
Circulating blood cells function as a surveillance system for damaged tissue in Drosophila larvae. PNAS 105 , 10017-10022.
... the mechanism of blood-cell recruitment to larval wound sites. First, the small number of tissue-bound blood cells in the vicinity of the wound site was not sufficient to account for the large number of cells that rapidly accumulated at the wound during the inflammatory response (Fig. 2 B, C, and I) ...
... the mechanism of blood-cell recruitment to larval wound sites. First, the small number of tissue-bound blood cells in the vicinity of the wound site was not sufficient to account for the large number of cells that rapidly accumulated at the wound during the inflammatory response (Fig. 2 B, C, and I) ...
Cell culture
Cell culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. In practice, the term ""cell culture"" now refers to the culturing of cells derived from multicellular eukaryotes, especially animal cells, in contrast with other types of culture that also grow cells, such as plant tissue culture, fungal culture, and microbiological culture (of microbes). The historical development and methods of cell culture are closely interrelated to those of tissue culture and organ culture. Viral culture is also related, with cells as hosts for the viruses. The laboratory technique of maintaining live cell lines (a population of cells descended from a single cell and containing the same genetic makeup) separated from their original tissue source became more robust in the middle 20th century.