biochemical investigation into initiation of fatty acid synthesis in the
... parasite T. brucei. In my dissertation, I addressed various aspects of the regulation of TbACC, which catalyzes the first committed step in FA synthesis. In the second chapter, I hypothesized that TbACC is regulated in response to environmental lipids. I examined changes in TbACC RNA, protein abunda ...
... parasite T. brucei. In my dissertation, I addressed various aspects of the regulation of TbACC, which catalyzes the first committed step in FA synthesis. In the second chapter, I hypothesized that TbACC is regulated in response to environmental lipids. I examined changes in TbACC RNA, protein abunda ...
PFK - ePrints USM
... optimum growth temperature in the range of 10 oC to 20 oC (Morita, 1975). They can be contrasted with thermophiles, which thrive at unusually hot temperatures. Despite the fact that a much greater proportion of the earth environment is cold rather than hot, much less is known about psychrophilic, co ...
... optimum growth temperature in the range of 10 oC to 20 oC (Morita, 1975). They can be contrasted with thermophiles, which thrive at unusually hot temperatures. Despite the fact that a much greater proportion of the earth environment is cold rather than hot, much less is known about psychrophilic, co ...
Studies Into the Allosteric Regulation of ADP
... case that just one subunit type is catalytic.1,5 The enzyme was first identified in 1962 by Espada, at the University of Buenos Aires, Argentina.6 The enzyme requires a divalent metal ion, such as Mg2+ or Mn2+. Although the enzyme catalyzes both the forward (ADPGlc synthesis) and reverse reactions ( ...
... case that just one subunit type is catalytic.1,5 The enzyme was first identified in 1962 by Espada, at the University of Buenos Aires, Argentina.6 The enzyme requires a divalent metal ion, such as Mg2+ or Mn2+. Although the enzyme catalyzes both the forward (ADPGlc synthesis) and reverse reactions ( ...
THE EXTRACTION OF PAPAIN FROM PAPAYA LEAVES NUR
... solution has good temperature stability, the solution stability is pH dependent. Papain solutions are unstable under acidic condition such as pH 2.8 and will result in significant loss of activity. Previous research had shown that papain extracted from latex will exhibit at low pH a partially struct ...
... solution has good temperature stability, the solution stability is pH dependent. Papain solutions are unstable under acidic condition such as pH 2.8 and will result in significant loss of activity. Previous research had shown that papain extracted from latex will exhibit at low pH a partially struct ...
Biochemical characterization of the initial steps of the Kennedy
... GPI (glycosylphosphatidylinositol) anchor that is required for membrane attachment of cell-surface proteins, most notably the variant-surface glycoproteins. The de novo synthesis of GPEtn and GPCho starts with the generation of phosphoethanolamine and phosphocholine by ethanolamine and choline kinas ...
... GPI (glycosylphosphatidylinositol) anchor that is required for membrane attachment of cell-surface proteins, most notably the variant-surface glycoproteins. The de novo synthesis of GPEtn and GPCho starts with the generation of phosphoethanolamine and phosphocholine by ethanolamine and choline kinas ...
Functional characterization of the Mycobacterium
... resulting recombinant protein was used for all assays described in this paper, except the PepChip assay. The DNA fragments encoding recombinant PepE and EmbR were generated using various primers containing the additional 4 bp sequence 59-CACC-39 necessary for directional cloning on the 59 end (Table ...
... resulting recombinant protein was used for all assays described in this paper, except the PepChip assay. The DNA fragments encoding recombinant PepE and EmbR were generated using various primers containing the additional 4 bp sequence 59-CACC-39 necessary for directional cloning on the 59 end (Table ...
C H A P
... of the lipase(s) produced by this bacterium remain almost unknown, since only related enzymes such as H. pylori phospholipases A2 and C have been cloned and/or characterized (Weitkamp et al., 1993; Dorrell et al., 1999; General Introduction 3.4.2). Therefore, this chapter is focused on the isolation ...
... of the lipase(s) produced by this bacterium remain almost unknown, since only related enzymes such as H. pylori phospholipases A2 and C have been cloned and/or characterized (Weitkamp et al., 1993; Dorrell et al., 1999; General Introduction 3.4.2). Therefore, this chapter is focused on the isolation ...
Protein O-linked β-N-acetylglucosamine
... glycosylation in that it is restricted to the cytoplasm, nucleus and mitochondria and it is not extended into complex elongated structures; it also exhibits parallels with protein phosphorylation, in that it responds to acute stimuli, alters protein function and enzyme activity and modifies the same ...
... glycosylation in that it is restricted to the cytoplasm, nucleus and mitochondria and it is not extended into complex elongated structures; it also exhibits parallels with protein phosphorylation, in that it responds to acute stimuli, alters protein function and enzyme activity and modifies the same ...
Aminolaevulinic acid synthase of Rhodobacter capsulatus: high
... glycine substrate. After condensation with succinyl-CoA and the indicative formation of an α-amino-β-oxoadipate intermediate, this carboxy group is released as CO2 . Consequently, this active site asparagine residue might be the crucial residue controlling ALAS catalysis. We generated two variants o ...
... glycine substrate. After condensation with succinyl-CoA and the indicative formation of an α-amino-β-oxoadipate intermediate, this carboxy group is released as CO2 . Consequently, this active site asparagine residue might be the crucial residue controlling ALAS catalysis. We generated two variants o ...
Purification and Some: Characteristics of a Monomeric Racemase
... the second base of the two-base model proposed by Adams (16), with the Lys 39 residue of the other subunit being the first base. Both Tyr 265 and Lys 39 are conserved in all alanine racemases whose amino acid sequences are known (13). Given that there are very few reports on monomeric alanine racema ...
... the second base of the two-base model proposed by Adams (16), with the Lys 39 residue of the other subunit being the first base. Both Tyr 265 and Lys 39 are conserved in all alanine racemases whose amino acid sequences are known (13). Given that there are very few reports on monomeric alanine racema ...
The shikimate pathway in apicomplexan parasites: Implications for
... gondii DAHPS is significantly larger than the previously reported type II enzymes. This is caused by numerous insertions into the protein sequence (21). To the best of our knowledge no biochemical information of an apicomplexan DAHPS is presently available except that DAHPS activity was observed in ...
... gondii DAHPS is significantly larger than the previously reported type II enzymes. This is caused by numerous insertions into the protein sequence (21). To the best of our knowledge no biochemical information of an apicomplexan DAHPS is presently available except that DAHPS activity was observed in ...
Platelet isolation and incubation with LDL - HAL
... degree of LDL oxidation. We show that the incubation of LDL with glucose did not change their anti-/pro-oxidant status corroborating that glycation alone is insufficient to promote LDL oxidation (14). Moreover, glycated LDL had no stimulating effects on platelets. Although one study reported that gl ...
... degree of LDL oxidation. We show that the incubation of LDL with glucose did not change their anti-/pro-oxidant status corroborating that glycation alone is insufficient to promote LDL oxidation (14). Moreover, glycated LDL had no stimulating effects on platelets. Although one study reported that gl ...
Life in the slow lane: molecular mechanisms of estivation
... produce cocoons made of multiple layers of shed skin (Loveridge and Withers, 1981; Pinder et al., 1992). Water loss is also retarded by colligative means by elevating the osmolality of body fluids via the production of high concentrations of solutes. Urea is used for this purpose by various fish, am ...
... produce cocoons made of multiple layers of shed skin (Loveridge and Withers, 1981; Pinder et al., 1992). Water loss is also retarded by colligative means by elevating the osmolality of body fluids via the production of high concentrations of solutes. Urea is used for this purpose by various fish, am ...
Purification and Characterization of
... Because H. carterae PRK activity was insensitive to NADH but was stimulated by dithiothreitol, it appears that the enzyme may require a thioredoxin/ferredoxin rather than a metabolite mode of regulation. Kinetic analysis of this enzyme demonstrated Michaelis constant values of ribulose-5-phosphate ( ...
... Because H. carterae PRK activity was insensitive to NADH but was stimulated by dithiothreitol, it appears that the enzyme may require a thioredoxin/ferredoxin rather than a metabolite mode of regulation. Kinetic analysis of this enzyme demonstrated Michaelis constant values of ribulose-5-phosphate ( ...
709_2010_211_MOESM2_ESM - Springer Static Content Server
... ARABIDOPSIS HISTIDINE KINASE 3: Important regulator of vascular tissue development in Arabidopsis thaliana shoots. Encodes a histidine kinases, a cytokinin receptor that controls cytokinin-mediated leaf longevity through a specific phosphorylation of the response regulator, ARR2. ...
... ARABIDOPSIS HISTIDINE KINASE 3: Important regulator of vascular tissue development in Arabidopsis thaliana shoots. Encodes a histidine kinases, a cytokinin receptor that controls cytokinin-mediated leaf longevity through a specific phosphorylation of the response regulator, ARR2. ...
patrick_tb_ch18b
... Feedback: Aromatase is an enzyme which converts androgens to oestrogens. Androgens are male hormones that are responsible for the development of male characteristics. Oestrogens (or estrogens) are female hormones that are responsible for the development of female characteristics. These are character ...
... Feedback: Aromatase is an enzyme which converts androgens to oestrogens. Androgens are male hormones that are responsible for the development of male characteristics. Oestrogens (or estrogens) are female hormones that are responsible for the development of female characteristics. These are character ...
488KB - The Doudna Lab - University of California, Berkeley
... and perfect substrates from both ends, pro[dsRNA] (µM) [dsRNA] (µM) ducing a mixture of 25- or 26-nt and 11- or 12-nt products from each substrate (Fig. 4e). Figure 3 Kinetic measurements of Dicer surface mutants. (a) Steady-state velocity of wild-type G. intestinalis Dicer as a function of substrat ...
... and perfect substrates from both ends, pro[dsRNA] (µM) [dsRNA] (µM) ducing a mixture of 25- or 26-nt and 11- or 12-nt products from each substrate (Fig. 4e). Figure 3 Kinetic measurements of Dicer surface mutants. (a) Steady-state velocity of wild-type G. intestinalis Dicer as a function of substrat ...
The proofreading mechanism of isoleucyl
... similar to the cognate one, and smaller, bind to a first recognition site. Then there is a second site on the enzyme, a hydrolysis site, in which only the smaller non‐cognate substrate fits. This would explain why e.g. valine is rejected by IRS. In 1998 the publication of crystal str ...
... similar to the cognate one, and smaller, bind to a first recognition site. Then there is a second site on the enzyme, a hydrolysis site, in which only the smaller non‐cognate substrate fits. This would explain why e.g. valine is rejected by IRS. In 1998 the publication of crystal str ...
Bioretrosynthetic Construction of a Non
... was also a friend and graduate student mentor, actively answering my questions, discussing interesting enzyme engineering thoughts and showing me the ropes for designing protocols for experiments in directed evolution and biosynthesis assays. I am thankful for him passing along his knowledge and exp ...
... was also a friend and graduate student mentor, actively answering my questions, discussing interesting enzyme engineering thoughts and showing me the ropes for designing protocols for experiments in directed evolution and biosynthesis assays. I am thankful for him passing along his knowledge and exp ...
- BioMedSearch
... but also inactivate PEPS while the pyruvate dehydrogenase complex would be active. In contrast, at low ADP concentrations, which would also indicate high ATP concentrations, PEPS would be activated and the E1 component of the PDH complex inhibited which would favour the anabolic conversion of pyruva ...
... but also inactivate PEPS while the pyruvate dehydrogenase complex would be active. In contrast, at low ADP concentrations, which would also indicate high ATP concentrations, PEPS would be activated and the E1 component of the PDH complex inhibited which would favour the anabolic conversion of pyruva ...
Malonate decarboxylase of Pseudomonas putida is composed of
... pneumoniae [3,6], Acinetobacter calcoaceticus, P. £uorescens, and P. putida [7,8]. The enzymes from Klebsiella, Acinetobacter, and Pseudomonas are composed of four subunits K, L, Q, and N. The N subunit is the acyl-carrier protein (ACP) being responsible for the reaction sequence on cyclic decarboxy ...
... pneumoniae [3,6], Acinetobacter calcoaceticus, P. £uorescens, and P. putida [7,8]. The enzymes from Klebsiella, Acinetobacter, and Pseudomonas are composed of four subunits K, L, Q, and N. The N subunit is the acyl-carrier protein (ACP) being responsible for the reaction sequence on cyclic decarboxy ...
Active site mapping, biochemical properties and
... effective only against T. b. gambiense and not T. b. rhodesiense. With melarsopol, a serious reactive encephalopathy may result in 5– 10% of patients treated with a 1–5% mortality. The sole new drug is eflornithine (DL-a-difluoromethylornithine, DFMO), a selective inhibitor of ornithine decarboxylas ...
... effective only against T. b. gambiense and not T. b. rhodesiense. With melarsopol, a serious reactive encephalopathy may result in 5– 10% of patients treated with a 1–5% mortality. The sole new drug is eflornithine (DL-a-difluoromethylornithine, DFMO), a selective inhibitor of ornithine decarboxylas ...
Flux limitations in the ortho pathway of benzoate
... and resuspended in Tris buffer. The cells were placed in a biological oxygen monitor (YSI 5300, Yellow Springs) with 3 ml of the same buffer containing 1 mM benzoate. Blanks without benzoate were prepared for each assay. B120 activity was expressed as mmol benzoate consumed (g cell dry wt)-l h-' and ...
... and resuspended in Tris buffer. The cells were placed in a biological oxygen monitor (YSI 5300, Yellow Springs) with 3 ml of the same buffer containing 1 mM benzoate. Blanks without benzoate were prepared for each assay. B120 activity was expressed as mmol benzoate consumed (g cell dry wt)-l h-' and ...
Specific features of glycogen metabolism in the liver
... The glycogen fraction contains only Ser}Thr protein phosphatases of type-1, termed PP-1G (glycogen-associated PP1) (Table 1). They consist of a catalytic subunit (37}38 kDa) and a glycogen-binding G-subunit. Four structurally related mammalian G-subunits have been cloned, three of which are present ...
... The glycogen fraction contains only Ser}Thr protein phosphatases of type-1, termed PP-1G (glycogen-associated PP1) (Table 1). They consist of a catalytic subunit (37}38 kDa) and a glycogen-binding G-subunit. Four structurally related mammalian G-subunits have been cloned, three of which are present ...
Journal of Bacteriology
... considerable amount of activity was lost during incubation of the enzyme for 10 min at this temperature, probably because cofactors or substrates or both, which may protect the enzyme, were lacking. We were surprised to find that the specific activity of the L-alanine adding enzyme of E. coli strain ...
... considerable amount of activity was lost during incubation of the enzyme for 10 min at this temperature, probably because cofactors or substrates or both, which may protect the enzyme, were lacking. We were surprised to find that the specific activity of the L-alanine adding enzyme of E. coli strain ...
Ultrasensitivity
In molecular biology, ultrasensitivity describes an output response that is more sensitive to stimulus change than the hyperbolic Michaelis-Menten response. Ultrasensitivity is one of the biochemical switches in the cell cycle and has been implicated in a number of important cellular events, including exiting G2 cell cycle arrests in Xenopus laevis oocytes, a stage to which the cell or organism would not want to return.Ultrasensitivity is a cellular system which triggers entry into a different cellular state. Ultrasensitivity gives a small response to first input signal, but an increase in the input signal produces higher and higher levels of output. This acts to filter out noise, as small stimuli and threshold concentrations of the stimulus (input signal) is necessary for the trigger which allows the system to get activated quickly. Ultrasensitive responses are represented by sigmoidal graphs, which resemble cooperativity. Quantification of ultrasensitivity is often approximated by the Hill equation (biochemistry):Response= Stimulus^n/(EC50^n+Stimulus^n)Where Hill's coefficient (n) may represent quantitative measure of ultrasensitive response.