C.Prinz, J.O. Tegenfeldt, R.H. Austin, E.C. Cox, J.C. Sturm, "Bacterial chromosome extraction and isolation," Lab Chip, 2, pp. 207-212 (2002).
... simply need to control the osmotic conditions. The lysozymetreated E. coli are stored in a high sucrose concentration solution. The sucrose (which is not membrane-permeant) ensures a high osmotic pressure surrounding medium in which the cell will swell but not lyse (spheroplasts). In order to lyse t ...
... simply need to control the osmotic conditions. The lysozymetreated E. coli are stored in a high sucrose concentration solution. The sucrose (which is not membrane-permeant) ensures a high osmotic pressure surrounding medium in which the cell will swell but not lyse (spheroplasts). In order to lyse t ...
Development of Next-Generation Optical Neural Silencers
... While all members of the opsin superfamily share a highly characteristic seven transmembrane alpha-helical structure and retinal chromophore attached to a conserved lysine residue (Figure 2), primary sequence alignments divide them into two independent protein families: the type 1 opsins which play ...
... While all members of the opsin superfamily share a highly characteristic seven transmembrane alpha-helical structure and retinal chromophore attached to a conserved lysine residue (Figure 2), primary sequence alignments divide them into two independent protein families: the type 1 opsins which play ...
Chapter 13: Characterizing and Classifying Viruses, Viroids, and
... A virus is a minuscule, acellular, infectious agent having one or several pieces of nucleic acid (DNA or RNA, never both). They have no plasma membrane (some have a membrane like envelope), cytosol, or organelles. They are not capable of metabolic activity on their own, outside of the cell, calle ...
... A virus is a minuscule, acellular, infectious agent having one or several pieces of nucleic acid (DNA or RNA, never both). They have no plasma membrane (some have a membrane like envelope), cytosol, or organelles. They are not capable of metabolic activity on their own, outside of the cell, calle ...
Bacillus anthracis produces membrane-derived vesicles containing biologically active toxins
... Many Gram-negative pathogenic bacterial species, including Pseudomonas aeruginosa, produce vesicles that contain toxins or other virulence factors and, in several cases, vesicles have been proposed to be vehicles for toxin delivery to eukaryotic cells (14– 17). Significantly less is known about the r ...
... Many Gram-negative pathogenic bacterial species, including Pseudomonas aeruginosa, produce vesicles that contain toxins or other virulence factors and, in several cases, vesicles have been proposed to be vehicles for toxin delivery to eukaryotic cells (14– 17). Significantly less is known about the r ...
An in silico analysis of the mitochondrial protein import apparatus of
... structures, the proposed presence of cytosolic targeting factors and even targeting of mRNA to the surface of organelles, may all combine to achieve the observed specificity of protein targeting [21,22]. There is a mechanistic difference between recognition of targeting signals by preprotein recepto ...
... structures, the proposed presence of cytosolic targeting factors and even targeting of mRNA to the surface of organelles, may all combine to achieve the observed specificity of protein targeting [21,22]. There is a mechanistic difference between recognition of targeting signals by preprotein recepto ...
Thyroglobulin, the major and obligatory
... lysosomal compartment. This compartment is reached, however, by distinctly different pathways: in thyroid follicle cells, newly synthesized lysosomal enzymes do not appear as a secretory constituent in the follicle lumen and are transported to lysosomes presumably on a direct, intracellular route. B ...
... lysosomal compartment. This compartment is reached, however, by distinctly different pathways: in thyroid follicle cells, newly synthesized lysosomal enzymes do not appear as a secretory constituent in the follicle lumen and are transported to lysosomes presumably on a direct, intracellular route. B ...
golgi apparatus, gerl, and lysosomes of neurons in rat dorsal root
... Electron microscope evidence from many laboratories suggests that in a wide variety of animal and plant cell types condensation of secretory and lysosomal proteins occurs in the saccules (cisternae) of the Golgi apparatus . The proteins are packaged in vesicles or vacuoles whose membranes are derive ...
... Electron microscope evidence from many laboratories suggests that in a wide variety of animal and plant cell types condensation of secretory and lysosomal proteins occurs in the saccules (cisternae) of the Golgi apparatus . The proteins are packaged in vesicles or vacuoles whose membranes are derive ...
Full Text
... Falkenburg et al., 1988; Pal and Murakami. 1988; Nothwang et al., 1992a). are composed of protein and small RNA. Prior to the finding that these particles have protease activity, they were characterized as facul1ative RNP particles (pro somes) associated with the ribosome-free repressed population o ...
... Falkenburg et al., 1988; Pal and Murakami. 1988; Nothwang et al., 1992a). are composed of protein and small RNA. Prior to the finding that these particles have protease activity, they were characterized as facul1ative RNP particles (pro somes) associated with the ribosome-free repressed population o ...
Transport of Storage Proteins to Protein Storage Vacuoles Is
... suggests that the vesicles with a density of 1.24 g/cm 3 mediate the transport of major storage proteins to protein storage vacuoles in maturing seeds of pumpkin and castor bean. However, the origin of these vesicles remains unknown. The ultrastructure of these vesicles, which includes an electron-d ...
... suggests that the vesicles with a density of 1.24 g/cm 3 mediate the transport of major storage proteins to protein storage vacuoles in maturing seeds of pumpkin and castor bean. However, the origin of these vesicles remains unknown. The ultrastructure of these vesicles, which includes an electron-d ...
Plant mitochondria contain the protein translocase subunits TatB
... excluding plant species were carried out with At5g43680 the only hits were bacterial TatB proteins (data not shown). Therefore, we believe that At5g43680 is a TatB-like protein. This is also supported by basic sequence comparisons as At5g43680 shows a 14% identity and 29% similarity to EcTatB in com ...
... excluding plant species were carried out with At5g43680 the only hits were bacterial TatB proteins (data not shown). Therefore, we believe that At5g43680 is a TatB-like protein. This is also supported by basic sequence comparisons as At5g43680 shows a 14% identity and 29% similarity to EcTatB in com ...
Pharmacology
... • prevents drug from entering tissue • slows Figure 21.3 © 2004 Wadsworth – Thomson Learning ...
... • prevents drug from entering tissue • slows Figure 21.3 © 2004 Wadsworth – Thomson Learning ...
Similarities and differences in the responses of microorganisms to
... differ greatly in such a wide range of organisms in which the composition of the outer cell layers might have a limiting role, albeit for different reasons. The possible role of yeast cell walls in modifying cellular response to CHX has been studied (Table 2).30 The relative porosity (RP) and thickn ...
... differ greatly in such a wide range of organisms in which the composition of the outer cell layers might have a limiting role, albeit for different reasons. The possible role of yeast cell walls in modifying cellular response to CHX has been studied (Table 2).30 The relative porosity (RP) and thickn ...
13 Copy of EAR final2012-09-15 05:175.8 MB
... • Describe the parts of the external ear: auricle and external auditory meatus. • Identify the boundaries of the middle ear : roof, floor and four walls (anterior, posterior, medial and lateral). • Define the contents of the tympanic cavity: • I. Ear ossicles,: (malleus, incus and stapes) • II. Musc ...
... • Describe the parts of the external ear: auricle and external auditory meatus. • Identify the boundaries of the middle ear : roof, floor and four walls (anterior, posterior, medial and lateral). • Define the contents of the tympanic cavity: • I. Ear ossicles,: (malleus, incus and stapes) • II. Musc ...
wrzaczek_ptms
... Post-translational modification (PTM) refers to the covalent and generally enzymatic modification of proteins during or after protein biosynthesis. (source: www.wikipedia.org) ...
... Post-translational modification (PTM) refers to the covalent and generally enzymatic modification of proteins during or after protein biosynthesis. (source: www.wikipedia.org) ...
Mitochondrion and Chloroplast Regulation of Plant Programmed
... proteins (Bcl-2 and Bcl-xL) [56]. Under normal conditions, mitochondrial outer membrane integrity is maintained through a balance between these pro- and antiapoptotic proteins, and proteins such as cyt c are prevented from being released from the mitochondria. There is an endoplasmic reticulum-locat ...
... proteins (Bcl-2 and Bcl-xL) [56]. Under normal conditions, mitochondrial outer membrane integrity is maintained through a balance between these pro- and antiapoptotic proteins, and proteins such as cyt c are prevented from being released from the mitochondria. There is an endoplasmic reticulum-locat ...
molecular mechanisms of weak organic acid preservative
... aqueous phase (koff). Concepts and drawing taken and modified from [15] ...
... aqueous phase (koff). Concepts and drawing taken and modified from [15] ...
Increased Susceptibility of the Sickle Cell Membrane
... normal and sickle cell membrane Ca2+ + MgF+-ATPase. Treatment of white erythrocyte membranes with tBHP (0.5 mmol/L) for 30 minutes did not result in significant loss of enzyme activity or formation of MDA. Therefore, to assess the effects of ascorbate and desferal on normal membranes, oxidation was ...
... normal and sickle cell membrane Ca2+ + MgF+-ATPase. Treatment of white erythrocyte membranes with tBHP (0.5 mmol/L) for 30 minutes did not result in significant loss of enzyme activity or formation of MDA. Therefore, to assess the effects of ascorbate and desferal on normal membranes, oxidation was ...
A novel protein transport system involved in the biogenesis of
... no longer an acceptable substrate for the Sec pathway. One possible way to circumvent this problem is to transport protein and cofactor across the cytoplasmic membrane separately, and then carry out cofactor insertion in the periplasm. This is the solution adopted for proteins with some types of cof ...
... no longer an acceptable substrate for the Sec pathway. One possible way to circumvent this problem is to transport protein and cofactor across the cytoplasmic membrane separately, and then carry out cofactor insertion in the periplasm. This is the solution adopted for proteins with some types of cof ...
biography: edwin cohn
... At around the time World War II broke out, Dr. Cohn helped to develop a blood fractionation process, which separates the different proteins in blood plasma. This separation of human blood plasma components offered valuable medical treatments. Specifically, the fraction of serum albumin helped to sav ...
... At around the time World War II broke out, Dr. Cohn helped to develop a blood fractionation process, which separates the different proteins in blood plasma. This separation of human blood plasma components offered valuable medical treatments. Specifically, the fraction of serum albumin helped to sav ...
Apoptosis, Necrosis, and Cell Viability Assays
... TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyzes the addition of label ...
... TUNEL (terminal deoxynucleotidyl transferase (TdT) mediated dUTP nick-end labeling) is highly selective for the detection of apoptotic cells, but not necrotic cells or cells with DNA strand breaks resulting from irradiation or drug treatment. In this assay, TdT enzyme catalyzes the addition of label ...
Non-equilibration of hydrostatic pressure in blebbing cells Guillaume T. Charras
... or viscous liquid10 require that hydrostatic pressure equilibrates essentially instantaneously over the whole cell. Here, we use cell blebs as reporters of local pressure in the cytoplasm. When we locally perfuse blebbing cells with cortex-relaxing drugs to dissipate pressure on one side, blebbing c ...
... or viscous liquid10 require that hydrostatic pressure equilibrates essentially instantaneously over the whole cell. Here, we use cell blebs as reporters of local pressure in the cytoplasm. When we locally perfuse blebbing cells with cortex-relaxing drugs to dissipate pressure on one side, blebbing c ...
