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The Lower Respiratory Tract Histology
The Lower Respiratory Tract Histology

... the carbonic anhydrase enzyme which is present within the erythrocytes. - The cells that form the respiratory membrane: - Type 1 cells (pneumocytes type 1): they form 97% of the alveolar wall, they are simple squamous cells, they are extremely attenuated cells, and their thickness is about 25nm. The ...
C - Aptagen
C - Aptagen

... TPP tissue culture dish, were exposed to TAMRA labeled G12 library (3 ml of the unbound fraction after (-) Mutant selection), for 30 minutes at 370C. The excess library was aspirated from the dish; cells were washed twice with 5 mL wash buffer (1X PBS supplemented with 4.5 mg/mL glucose and 5mM MgCl ...
Tissues
Tissues

... Leukocytes: White blood cells are normally  found in small numbers in healthy connective  tissue but migrate in significant numbers uring infection when they play an important part in  tissue defence. Lymphocytes synthesize and secrete antibodies  into the blood in the presence of foreign  material ...
RSPT 1207 Cardiopulmonary Anatomy and Physiology
RSPT 1207 Cardiopulmonary Anatomy and Physiology

... GOBLET CELLS – Pseudo-stratified columnar ciliated epithelium that has lost its cilia, has gained the ability to secret mucous Location: scattered throughout the ciliated cells at a ratio of 1:5, or one goblet cell for every 5 ciliated cells Goblet cells will increase with prolonged irritation ...
Blood is composed of a fluid portion (plasma)
Blood is composed of a fluid portion (plasma)

... - erythropoietin stimulates more rapid division of stem cells and production of hemoglobin in erythroblast - more rbc are produced, which increase oxygen transported in blood *What does the hormone erythropoietin do to maintain homeostasis? Where is it produced and what triggers its production? *Why ...
Animal Histology
Animal Histology

... Animal Histology: Form and Function ...
Notes 1
Notes 1

... Since cells of meristematic tissue are highly active so they have dense cytoplasm. Vacuole is absent in these cells. These cells have high metabolic activity and rate of respiration. They grow and divide repeatedly. Functions: They take part in growth, produce new leaves, stem, flower, fruit etc , h ...
Mechanism of Induction: Induced Pluripotent Stem Cells (iPSCs)
Mechanism of Induction: Induced Pluripotent Stem Cells (iPSCs)

... human dermal fibroblasts from the facial dermis of Caucasian woman and transduced them with retroviral vector containing Oct4, Sox2, Klf4, and cMyc genes [7]. Since then, newer approaches have been developed to reprogram a somatic cell to an iPSC, which includes exploring the four different factors, ...
Is it a Good Idea to upgrade our DNA
Is it a Good Idea to upgrade our DNA

... But Perry’s work added a unique flourish. He did the editing not in a one-cell mouse embryo – which is how most animal germ-line editing by Crispr has been done to date – but earlier, during the process of fertilisation, by injecting the Crispr components and the mouse sperm into the mouse egg at t ...
Unit 2 Multicellular Organisms Mr Gravell
Unit 2 Multicellular Organisms Mr Gravell

... In plants cells that divide are found only at meristems and the unspecialised cells produced can become any type of plant cell. Meristems are found • At the root and shoot tip – these produce new cells for increase in length of the root and shoot • Between the xylem and phloem – these meristems prod ...
Redekop et al in Pharmacoeconomics 2003
Redekop et al in Pharmacoeconomics 2003

... safety, no toxicology testing or evidence of efficacy was needed…In April 2001 there was the BSE scare and Germany put pressure on the EMEA to see Apligraf as a medicinal product…the product is very complex….. ...
ISCI/FRM/004 – hES Cell Details
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Plant Cell
Plant Cell

... function. Draw each organelle in the palm of each hand to create a cell. • Be creative and color each side. ...
Cells - Open Equal Free
Cells - Open Equal Free

... DNA holds the information that makes organisms, and their cells, different. Plant cells are different from animal cells in a way that is easy to see. All cells have cell membranes, but plants have harder walls that help them maintain their rigid shape. Animals have bones to keep them upright, but pl ...
Human Body study guide
Human Body study guide

... These questions/concepts serve as a guide to summarize all of the concepts we learned in Unit 3. Some of the concepts will be more prevalent (seen more) throughout the test compared to others. 1. The human body systems all focus on the concept of maintaining homeostasis. Explain, in your own words, ...
The respiratory system
The respiratory system

... a. Type I pneumocytes (type I alveolar cells) (1) cover about 95% of the alveolar surface and form part of the blood-gas barrier where exchange of oxygen and carbon dioxide occurs. (2) have an extremely thin cytoplasm that may be less than 80 nm thick (3) form tight junctions with adjacent cells. (4 ...
2.1 Cell Theory
2.1 Cell Theory

... stem cells. Totipotent means they are capable of being stimulated to become one of any type of cell. 9. Cells are stimulated using differentiation factors to become the type of cell required for therapy. 10. Therapy would require the transfer of the new healthy cell to the patient. In therapeutic cl ...
Laskers for 2001: Knockout mice and test-tube babies
Laskers for 2001: Knockout mice and test-tube babies

... Two principal developments in the 1980s made it possible clinical practice. The 2001 Lasker Clinical Medical Research Award honors for scientists to knock out specific genes in the germ line of mice. The first, pioneered by Martin Evans (then at Robert Edwards of Cambridge University in the UK for d ...
The Nephron
The Nephron

... environment provides attractive conditions for the growth of bacteria, viruses as well as protists and fungi. Some of these are helpful, such as those which live mutualistically within the gut to aid in digestion. However, some are pathogenic, causing diseases. • Immune Response Luckily, to protect ...
Winter 2016 USC Stem Cell Newsletter
Winter 2016 USC Stem Cell Newsletter

... ew researchers have studied how hematopoietic stem cells (HSCs) respond to infection—even though these stem cells give rise to the full battery of specialized immune cells, such as T cells and B cells. Adnan Chowdhury is venturing into this uncharted territory as the winner of the Hearst Fellowship, ...
Chapter 4- Tissues/Histology
Chapter 4- Tissues/Histology

... Primary tissues all formed by the 2nd month and rapid growth continues in prenatal period. Nerve cells stop by fetal period and cell division slows down in adult hood. Stem cells are maintained throughout life for regeneration. Good nutrition and circulation helps in tissue regeneration. Tissue repa ...
Tissues and Integument
Tissues and Integument

... keratinocytes 4) Synthesize keratin in mid layers 5) By the time these cells are pushed up into the stratum corneum, they are little more than plasma membranes packed with keratin; they are dead and are exfoliated 6) Function: protection b. Stratum basale 1) Single layer of cuboidal or low columnar ...
Form 3 Biology End Of Term 3 Paper 2
Form 3 Biology End Of Term 3 Paper 2

... b) Suppose that each group of cells was placed in a highly concentrated sucrose solution. Describe briefly what would happen in each case. (4 marks) ...
Sub-topics include: 3.1 Cells, Tissues and Organs 3.2 Stem Cells
Sub-topics include: 3.1 Cells, Tissues and Organs 3.2 Stem Cells

... after they are instructed to specialize. Every cell in the body, for example, is derived from first few stem cells formed in the early stages of embryonic development. Therefore, stem cells extracted from embryos can be made to become any desired cell type. This property makes stem cells powerful en ...
Live imaging genetically-encoded fluorescent proteins in embryonic
Live imaging genetically-encoded fluorescent proteins in embryonic

... self-renewal and inhibit differentiation. Three key molecular players associated with pluripotency have been identified to date, they include: (1) the POU domain-containing factor OCT4, (2) the HMG domaincontaining factor SOX2, and (3) the homeobox domain-containing factor NANOG. Each of these genes ...
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Induced pluripotent stem cell



Induced pluripotent stem cells (also known as iPS cells or iPSCs) are a type of pluripotent stem cell that can be generated directly from adult cells. The iPSC technology was pioneered by Shinya Yamanaka’s lab in Kyoto, Japan, who showed in 2006 that the introduction of four specific genes encoding transcription factors could convert adult cells into pluripotent stem cells. He was awarded the 2012 Nobel Prize along with Sir John Gurdon ""for the discovery that mature cells can be reprogrammed to become pluripotent."" Pluripotent stem cells hold great promise in the field of regenerative medicine. Because they can propagate indefinitely, as well as give rise to every other cell type in the body (such as neurons, heart, pancreatic, and liver cells), they represent a single source of cells that could be used to replace those lost to damage or disease.The most well-known type of pluripotent stem cell is the embryonic stem cell. However, since the generation of embryonic stem cells involves destruction (or at least manipulation) of the pre-implantation stage embryo, there has been much controversy surrounding their use. Further, because embryonic stem cells can only be derived from embryos, it has so far not been feasible to create patient-matched embryonic stem cell lines.Since iPSCs can be derived directly from adult tissues, they not only bypass the need for embryos, but can be made in a patient-matched manner, which means that each individual could have their own pluripotent stem cell line. These unlimited supplies of autologous cells could be used to generate transplants without the risk of immune rejection. While the iPSC technology has not yet advanced to a stage where therapeutic transplants have been deemed safe, iPSCs are readily being used in personalized drug discovery efforts and understanding the patient-specific basis of disease.Depending on the methods used, reprogramming of adult cells to obtain iPSCs may pose significant risks that could limit their use in humans. For example, if viruses are used to genomically alter the cells, the expression of oncogenes (cancer-causing genes) may potentially be triggered. In February 2008, scientists announced the discovery of a technique that could remove oncogenes after the induction of pluripotency, thereby increasing the potential use of iPS cells in human diseases. In April 2009, it was demonstrated that generation of iPS cells is possible without any genetic alteration of the adult cell: a repeated treatment of the cells with certain proteins channeled into the cells via poly-arginine anchors was sufficient to induce pluripotency. The acronym given for those iPSCs is piPSCs (protein-induced pluripotent stem cells).
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