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Visualizing the actin cytoskeleton in living plant cells using a photo
Visualizing the actin cytoskeleton in living plant cells using a photo

... filter LP 470). This resulted in rapid loss of green fluorescence (Figure 2D) with a concomitant increase in red fluorescence (Figure 2E, F). It is known that transient expression methods such as the DNA-coated gold-particle bombardment employed by us result in variability between experiments and va ...
The Plant Cell - Gunawardena Lab
The Plant Cell - Gunawardena Lab

... are the first signs of PCD and are followed closely by the appearance of TUNEL-positive nuclei. Although the cytological events resemble those seen during tracheary element differentiation, cell walls also must be degraded as part of the PCD process, thus providing the open “windows” of a mature lac ...
University of Zurich - Institute of Molecular Life Sciences
University of Zurich - Institute of Molecular Life Sciences

... and immortalized with human papilloma virus 16 E6 and E7 proteins (86) was obtained from Dr. F. Verrey (Institute of Physiology, University of Zurich, Zurich), and propagated in K1-medium. Human hematopoietic K562 cells were used as described (3). CHO cells stably expressing GFP-CD46 were generated ...
increased_number_of_red_blood_cells
increased_number_of_red_blood_cells

... circulation; this response of the spleen is usually momentary and is a response to stress or release of epinephrine, thus the name “transient polycythemia” • Absolute polycythemia—characterized by a “true” or “absolute” increase in the number of circulating red blood cells as a result of an increase ...
Slide 1
Slide 1

... • Strains in which there are deficiencies or inappropriate responses ...
NORMAL TISSUE GROWTH AND CELL PROLIFERATION
NORMAL TISSUE GROWTH AND CELL PROLIFERATION

... Ï pancreatic nodular hyperplasia in old dogs, cats and cattle. Ð adrenal cortical nodular hyperplasia in old dogs, cats and horses. Ñ thyroid nodular hyperplasia in old cats (one cause of hyperthyroidism). - this group of hyperplastic lesions occupies a nebulous position in the spectrum of abnormal ...
CHA-CAAreviewCC06
CHA-CAAreviewCC06

... double-strand breaks (DSBs), predominantly at the time of DNA replication, while the remaining 99% are repaired by essentially error-free mechanisms. Thus, on average, about 50 DSBs (“endogenous DSBs”) per nucleus (~0.8 DSBs per 108 bp) are generated during a single cell cycle in human cells.5 Recom ...
Howard County Public School System Essential Curriculum
Howard County Public School System Essential Curriculum

... Recognize that adaptations may include variations in structures, behaviors, or physiology, such as spiny leaves on a cactus, birdcalls, and antibiotic resistant bacteria. c. Recognize and describe that adaptation and speciation involve the selection of natural variations in a population. ...
Are mesenchymal stem cells in rheumatoid Open Access
Are mesenchymal stem cells in rheumatoid Open Access

... from blood-borne circulating MSCs into the synovial pool would not be surprising given that MSCs can be found in the circulation [30] and are likely to traffic across, home to and engraft in tissues and organs of the entire body. Origins may differ for MSCs found at distinct niche sites. The ontogen ...
Topic #6: Human Physiology
Topic #6: Human Physiology

... 3. These cells secrete a fluid which coats the inner surface of the alveoli 4. This fluid allows the oxygen to dissolve and then diffuse into the blood 5. Provides an area for the carbon dioxide to evaporate into the air 6. Pulmonary surfactant – the molecules are similar to phospholipids - hydrophi ...
Increased Number of Red Blood Cells
Increased Number of Red Blood Cells

... circulation; this response of the spleen is usually momentary and is a response to stress or release of epinephrine, thus the name “transient polycythemia” • Absolute polycythemia—characterized by a “true” or “absolute” increase in the number of circulating red blood cells as a result of an increase ...
Increased Number of Red Blood Cells (Polycythemia)
Increased Number of Red Blood Cells (Polycythemia)

... circulation; this response of the spleen is usually momentary and is a response to stress or release of epinephrine, thus the name ―transient polycythemia‖ • Absolute polycythemia—characterized by a ―true‖ or ―absolute‖ increase in the number of circulating red blood cells as a result of an increase ...
Ecol 183
Ecol 183

... or thin black line depending on how full the sac is. The ink is the pigment melanin, which is the pigment found within our skin cells and causes tanning and different skin tones. Squid expel the ink when they are threatened causing confusion to the predator while the squid escapes. 5. Locate the gil ...
Identification of a nuclear-localized nuclease from wheat cells
Identification of a nuclear-localized nuclease from wheat cells

... acid [6]. Among the caspases participating in apoptosis, upstream caspases are known as initiators, whereas downstream caspases, which include caspase 3 and 7, are known as executioners because their activity leads to DNA degradation, chromatin condensation and nuclear membrane blebbing [7]. Caspase ...
Ch 27 Fluid, Electrolytes, Acid/Base Summary 2014
Ch 27 Fluid, Electrolytes, Acid/Base Summary 2014

... The other one third is called EXTRACELLULAR FLUID. About 80% of extracellular fluid is found in between cells and is called INTERSTITIAL FLUID. The other 20% of extracellular fluid is blood plasma. Examples of interstitial fluid are: Lymphatic fluid, cerebrospinal fluid, GI tract fluids, synovial fl ...
Tomato LeAGP-1 is a plasma membrane-bound
Tomato LeAGP-1 is a plasma membrane-bound

... the five peak fractions using the LeAGP-1 antibody by ELISAs (data not shown) and by Western blotting (Fig. 3B) demonstrated that LeAGP-1 is only present in the PM-3 fraction as a deglycosylated protein with an apparent molecular weight of 48 kDa. Fluorescence microscopy of transgenic tobacco cells ...
Acute Painless Loss of Vision
Acute Painless Loss of Vision

... loss, night sweats, myalgia, PMR • Rapd, Swollen disc, can present as CRAO, BRAO or cranial nerve palsies. • High ESR, CRP and platelets. TABx 90% sensitivity • Rx: 3 days of I/V methylprednisolone 1g, followed by oral prednisolone 6080mg/ day. Taper treatment according to response. Aspirin helpful. ...
the test - One Day Enrichment
the test - One Day Enrichment

... 20. Requirements of humans do not include   A. water. B. foods. C. oxygen. D. light. E. pressure. ...
Wang YY, Kuang A, Russell SD, Tian HQ. 2006. In vitro fertilization
Wang YY, Kuang A, Russell SD, Tian HQ. 2006. In vitro fertilization

... Sperm cells are expected to fulfill the functions of recognition, adherence and fusion with the female gamete during fertilization. Each of these functions of the male gamete may be controlled by activation of a unique gene or network of genes. The isolation of such fertilization-related genes in spe ...
The Euglena
The Euglena

... called the reservoir. Color the reservoir grey and the flagellum black. The Euglena is unique in that it is both heterotrophic (must consume food) and autotrophic (can make its own food). Chloroplasts within the euglena trap sunlight that is used for photosynthesis, and can be seen as several rod li ...
Proteolysis in microfluidic droplets: an approach to
Proteolysis in microfluidic droplets: an approach to

... silicon wafers and degassed. The microchannels are 100 μm in depth, 250 μm in width and the length of reaction channels is 46 cm. After curing at 80 C for 2 hours, the PDMS cast was cut and peeled off from the wafers. Microfluidic channel inlets and outlets were stamped out using a biopsy punch wit ...
Frog (Rana tigrina)
Frog (Rana tigrina)

... Buccopharyngeal cavity The buccal cavity and pharynx together is called bucco-pharyngeal cavity. Buccopharyngeal cavity lies between upper jaw and lower jaws. Upper jaw is immovable but lower jaw is movable. Teeth – there are two types of teeth. Maxillary teeth- they are found in upper jaw. They are ...
- ISpatula
- ISpatula

... expected to segregate into the core of the micelles, while the hydrophilic component forms the corona or outer shell. The hydrophobic micelle core serves as a microenvironment for incorporation of various therapeutic compounds, while the corona can act as a stabilizing interface between the hydropho ...
The Anitschkow Prize in Atherosclerosis Research, 2013
The Anitschkow Prize in Atherosclerosis Research, 2013

... In 1989 Prof. Libby proposed a model for the immunopathogenesis of transplantation arteriopathy. His later experiments in genetically altered mice proved his original postulates of an alloimmune response to donor vascular cells with a central role of signaling due to interferon-gamma. This model has ...
- Wiley Online Library
- Wiley Online Library

... MET channel in vertebrate hair cells. Using 50 Hz sinusoidal stimulation, we recorded the MET current by stepping the membrane between 161 and +99 mV in 20 mV increments from OHCs of both Piezo1+/+ and Piezo1+/ mice before (Fig. 3A, B, left panels) and during the superfusion of 10 lmol/L extracellul ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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