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Permanent cell cycle exit in G2 phase after DNA damage in normal
Permanent cell cycle exit in G2 phase after DNA damage in normal

... activation of Cdk1, as no hypophosphorylated Cdk1 (isoform 1) could be detected with cyclin B1. In contrast, in ICRF-193-treated cells, cyclin B1 complexes contained a substantial fraction of hypophosphorylated and active Cdk1 (isoform 1 in Figure 4C). These results implied that, in E6 cells, the de ...
Phylum Arthropoda
Phylum Arthropoda

... lobsters, pill bug, and crayfishes Subphylum Chelicerates – horseshoe crabs and arachnids (spiders, ticks, scorpions) Subphylum Uniramia – insects, millipedes, and centipedes ...
Answer Key - Earl Haig Secondary School
Answer Key - Earl Haig Secondary School

... 34. Diffusion occurs through a selectively permeable membrane. Particles diffuse from areas of high concentration to areas of low concentration until they reach a point of equilibrium. Osmosis is the movement of water molecules across a selectively permeable membrane in response to water. Water move ...
Muscle Cells - Circulation Research
Muscle Cells - Circulation Research

... synthesis was minimal for normal cardiac muscle cells in the adult heart and much greater in cardiac muscle cells in the fetal heart. In vitro, similar results were obtained. Although more than 30% of the fetal cardiac muscle cells progressed through S phase of the cell cycle over the course of 24 h ...
Asymmetric Cell Divisions in the Early Embryo of the Leech
Asymmetric Cell Divisions in the Early Embryo of the Leech

... lacked a true coelom (Valentine and Collins 2000). If so, many of the features we associate with modern bilaterian animals may have arisen largely independently within these three lines. On the other hand, others have proposed that the urbilaterian was a segmented eucoelomate with well-developed sen ...
Tolerance of Immobilized Yeast Cells in Imidazolium
Tolerance of Immobilized Yeast Cells in Imidazolium

... excellent properties, such as nonvolatile, thermal stability, designability, reusage and solvability to hydrophobic substrate as well as being environmentally friendly (17). That is why they have been recognized as an excellent and promising biocatalysis medium, as an alternative to conventional vol ...
Photobleaching Substrates Characterized Using Fluorescence
Photobleaching Substrates Characterized Using Fluorescence

... Despite the evidence that Ku functions in NHEJ during repair of DSBs, its properties in intact cells are unclear. For example, little is known concerning the dynamics of Ku in intact nuclei as defined by its mobility and the rate at which it associates with substrates. Interestingly, recent studies ...
Histoarchitecture and scanning electron microscopic studies of the
Histoarchitecture and scanning electron microscopic studies of the

... special interest because they form part of the olfactory transduction mechanism, are stimulated by odour-bearing substances, and also enable the fish to detect food. The microvillous receptor cells might form a different olfactory transduction mechanism for pheromones or amino acids. Hansen et al. ( ...
Cork Cambium
Cork Cambium

... • Differentiated cells (from primary growth) revert to become undifferentiated cells, forming the lateral meristems (Cambium). (Latin cambire, “to exchange”. Cambium cells have “exchanged” their previous roles for a new role of dividing repeatedly for new growth.) • Vascular Cambium: produces second ...
IDENTIFICATION OF CD133+/NESTIN+ PUTATIVE CANCER STEM
IDENTIFICATION OF CD133+/NESTIN+ PUTATIVE CANCER STEM

... Aims. No effective treatment for lung cancer exists currently. One reason for this, is the development of drug resistance, assumed to be associated with cancer stem cell (CSCs) emergence within the tumour. This pilot study aimed to identify CSCs in 121 non-small cell lung cancer (NSCLC) patient samp ...
Cytoskeleton-Plasma Membrane-Cell Wall
Cytoskeleton-Plasma Membrane-Cell Wall

... of proteins immunologically related to both integrins and cadherins (Kaminskyj and Heath, 1994; Katembe et al., 1997; Barthou et al., 1998; Canut et al., 1998; Faik et al., 1998; Kiba et al., 1998; Baluška et al., 1999b; Labouré et al., 1999; Laval et al., 1999; Nagpal and Quatrano, 1999; Swatzell ...
The Lymphatic System - leavingcertbiology.net
The Lymphatic System - leavingcertbiology.net

... Chapter 32: The human lymphatic system Leaving Certificate Biology Higher Level ...
ANTIIDIOTYPIC IMMUNITY IN INTERSTITIAL NEPHRITIS II. Rats
ANTIIDIOTYPIC IMMUNITY IN INTERSTITIAL NEPHRITIS II. Rats

... followed by an intense mononuclear cell infiltrate. T h e cellular lesion is composed of several subsets of T lymphocytes, macrophages, and Ig + cells (4). Natural killer cells have also been observed in the murine form of disease (5). T h e relevant tubular antigen for disease is a glycoprotein exp ...
specimen collection
specimen collection

... diagnosis of viral respiratory infections but may also be submitted to diagnose pertussis, diphtheria, chlamydia infections, and candidiasis, as well as identify carriers of N. meningitidis or S. aureus. Throat swab specimens are generally collected to diagnose group A streptococcal pharyngitis or t ...
Connective Tissue
Connective Tissue

... after stretching; maintains pulsatile flow of blood through arteries; aids passive recoil of lungs following inspiration. Location: Walls of large arteries; within certain ligaments associated with the vertebral column; within the walls of the bronchial tubes. ...
ch4 Jeopardy-11 - Room 189`s Wiki at PACS
ch4 Jeopardy-11 - Room 189`s Wiki at PACS

... Which of the following statements about prokaryotic cells is FALSE? a. They have an extensive cytoskeleton. b. They have no lysosomes. c. They are composed of inclusions of many different substances used for energy. d. They use vacuoles for buoyancy. ANSWER BACK TO GAME ...
Plant Cells Contain Two Functionally Distinct
Plant Cells Contain Two Functionally Distinct

... al., 1992) cells. The processed mature form of aleurain colocalized with vacuolar enzyme markers in cell fractionation experiments (Holwerda et al., 1992). The enzymes responsible for processing proaleurain in barley aleurone cells have an acidic pH optimum (Holwerda et al., 1990), and aleurain puri ...
Distinct fluorescent pattern of KAT1::GFP in the plasma membrane of
Distinct fluorescent pattern of KAT1::GFP in the plasma membrane of

... similar to what we previously described for guard cell protoplasts (Hurst et al., 2004). In addition we found a radial distribution of KAT1::GFP clusters which was dependent on a close contact between the PM and the cell wall. In animal cells contacts of the PM with the ECM are mediated by substrate ...
Course Outline
Course Outline

... of his own design. He called them "animalcules" and published his observations in a series of letters to the Royal Society. The name bacterium was introduced much later, by Christian Gottfried Ehrenberg in 1838. Louis Pasteur demonstrated in 1859 that the fermentation process is caused by the growth ...
Grx5 Is a Mitochondrial Glutaredoxin Required for the
Grx5 Is a Mitochondrial Glutaredoxin Required for the

... following standard methods: aconitase, citrate synthase, malate dehydrogenase (Robinson et al., 1987), and succinate dehydrogenase (Munujos et al., 1993). Activities were expressed in units (nanomoles per minute) per milligram of cell protein. Extracts were prepared in 0.1 M Tris buffer, pH 8.1, plu ...
Cell adhesion in plants is under the control of
Cell adhesion in plants is under the control of

... (FADLox), a gene known to be responsive to pectins (Denoux et al., 2008; Kohorn et al., 2014). The expression level of FADLox was ∼5-fold higher in qua2-1 than in the wild type, qua2-1/esmd1-1 and esmd1-1 (Fig. 3B). This suggests that a pectin-related signal is induced in quasimodo, and repressed by ...
Mitosis and Polyploid Cell Formation
Mitosis and Polyploid Cell Formation

... for the genetic fidelity of the daughter cells. The first, DNA replica tion, correctly duplicates the genetic material and the second, mitosis, ensures that each daughter cell receives a complete set of chromo somes. These two phases are tightly coordinated such that the initia tion of one is depend ...
Kinetics of binding, uptake and degradation of live
Kinetics of binding, uptake and degradation of live

... target particle for phagocytosis. We have developed an assay using live bacteria expressing the fluorescent protein DsRed (DsRed-Ec), allowing us to assess both uptake and processing of bacteria by cells. When cells were shaken in suspension with DsRed-Ec, cells rapidly began to take up bacteria and ...
Structure–function relationships during secondary phloem
Structure–function relationships during secondary phloem

... the cell. Transmission electron microscopy showed parallel CMTs at both tangential walls of the cell, also arranged in a manner consistent with a helical arrangement (Figure 8). At this early stage of differentiation it was not possible to identify which cell type the element would become. In cells ...
The Plant Extracellular Matrix: News from the Cell`s
The Plant Extracellular Matrix: News from the Cell`s

... expression in roots, flowers, or seeds. DNA gel blot analysis suggests that four to eight genes may be present in cotton and four in rice. A considerable amount of the CelA sequence is unique to plants, and the functions of the different domains can only be surmised for now. The observation of more ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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