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DNA damage induced by mutagens in plant and human cell nuclei
DNA damage induced by mutagens in plant and human cell nuclei

... tobacco and leukocytes - 2.73 and 2.52, respectively. 0.4 mM MH and 0.1 mM MNU induced an increase in DNA damage; nevertheless, the level of DNA damage measured as TM was not significantly different in tobacco and leukocyte nuclei. With lower concentration of MH, the TM values were 8 and 9 for tobac ...
Fluid, Electrolyte, and Acid-Base Balance Body Water Content
Fluid, Electrolyte, and Acid-Base Balance Body Water Content

... Changes in plasma sodium levels affect: Plasma volume, blood pressure ICF and interstitial fluid volumes Renal acid-base control mechanisms are coupled to sodium ion transport Regulation of Sodium Balance: Aldosterone Sodium reabsorption 65% of sodium in filtrate is reabsorbed in the proximal tubule ...
Negative Feedback
Negative Feedback

... What this means is that the homeostatic variables are NOT kept rigidly fixed upon a single value. They are kept within a certain range, and when they exit that range – that’s when negative feedback loops turn on to bring them back. Is your body temperature always exactly 98.6F? ...
Biology 6 – Test 3 Study Guide
Biology 6 – Test 3 Study Guide

... 4. RBCs are eventually destroyed in the liver. This breaks down hemoglobin into bilirubin and releases it through bile into the small intestine (Fig. 12.5). Jaundice is poor release of bilirubin into bile, so it goes back into the blood stream and makes you yellow. ii. White blood cells (leukocytes) ...
Cytokines T Cells: Role of CD86 and
Cytokines T Cells: Role of CD86 and

... ␥␦ T cells, as assessed by induction of their proliferation and Th1 cytokine production as well as stimulation of cytotoxic activity against selected tumor cells (10, 11). In contrast to pyrophosphomonoesters, aminobiphosphonate-induced activation and clustering of human peripheral blood V␦2 T cells ...
Structural aspects of bulge formation during root hair initiation
Structural aspects of bulge formation during root hair initiation

... the der1 locus showed that it is mutated in the actin2 gene, corresponding well with the above drug studies. Aberrantly shaped bulges and young root hairs are characteristic also for other root hair mutants of Arabidopsis. For instance, rhd4 mutant shows irregularly thickened cell walls which can be ...
DIGESTION AND ABSORPTION OF LIPIDS
DIGESTION AND ABSORPTION OF LIPIDS

... DIGESTION OF LIPIDS ƒ Major dietary lipids are TGL, cholesterol & phospholipids. ƒ Normal Indian lipid diet contain 20-30g/day ƒ In developed countries, 60-150g/day ...
Microtubule and F-actin dynamics at the division site in living
Microtubule and F-actin dynamics at the division site in living

... stopped at metaphase and cytokinesis was disrupted. The extent of the side effects diminished with decreasing concentrations of probenecid, so that at 1 mM no inhibition of cellular processes occurred. Sequestration of Rh-phalloidin occurred in 1 mM probenecid, but at a much reduced rate compared to ...
Biology of Tooth Movement
Biology of Tooth Movement

... characteristics [8]: ...
PSEUDOMONAS
PSEUDOMONAS

... as carbon or nitrogen, becomes scarce or if the population becomes too dense, the bacteria can become stressed. They will enter a stasis phase, which is their equivalent of survival mode. The bacteria can survive in stasis until better growth conditions return. Sporulation Bacteria normally grow, ma ...
Untitled - University of Guelph
Untitled - University of Guelph

... isodiametric. How does a spherical cell get transformed into just about any other imaginable shape as it matures and occupies its position within the overall developmental pattern of a plant? The prosaic answer to the above question is that ‘differential growth shapes plant cells’. This phrase conve ...
Section 2
Section 2

... Cell biologists divide the eukaryotic cell into two major parts: the nucleus and the cytoplasm. The cytoplasm is the portion of the cell outside the nucleus. Slide 3 of 49 Copyright Pearson Prentice Hall ...
7-2 Eukaryotic Cell Structure
7-2 Eukaryotic Cell Structure

... Cell biologists divide the eukaryotic cell into two major parts: the nucleus and the cytoplasm. The cytoplasm is the portion of the cell outside the nucleus. Slide 3 of 49 Copyright Pearson Prentice Hall ...
7-2 Eukaryotic Cell Structure
7-2 Eukaryotic Cell Structure

... Cell biologists divide the eukaryotic cell into two major parts: the nucleus and the cytoplasm. The cytoplasm is the portion of the cell outside the nucleus. Slide 3 of 49 Copyright Pearson Prentice Hall ...
Characterization and Expression of Monosaccharide Transporters
Characterization and Expression of Monosaccharide Transporters

... same buffer as that of the prehybridization. The membranes were washed once in 2 SSC (1 SSC is 0.15 M NaCl and 0.015 M sodium citrate) containing 1% SDS for 15 min and twice in 0.2 SSC and 1% SDS at 15-min intervals at 65C. Membranes were exposed using a Fujix BAS2000 Bio-Imaging Analyzer (Fuji ...
Aluminum Toxicity Is Associated with
Aluminum Toxicity Is Associated with

... Potential mechanisms of Al toxicity measured as Al-induced inhibition of growth in cultured tobacco cells (Nicotiana tabacum, nonchlorophyllic cell line SL) and pea (Pisum sativum) roots were investigated. Compared with the control treatment without Al, the accumulation of Al in tobacco cells caused ...
The immunological synapse: a focal point for endocytosis and
The immunological synapse: a focal point for endocytosis and

... The precise site of signaling events leading to immune synapse formation has been controversial (for review see Dustin, 2009). Early signaling events have been shown to occur in peripheral microclusters in the pSMAC that coalesce into the cSMAC in both T and B cell synapses (Campi et al., 2005; Yoko ...
Tissue: The Living Fabric
Tissue: The Living Fabric

... storing functioning is greatly increased Adipocytes predominate tissue as little matrix is present Oil (fat) occupies most of cell volume Compression of the cell nucleus to one side gives it a name of “signet” cells Tissue is richly vascular owing to high ...
Ganeden Biotech Clinical Summaries - Nutra
Ganeden Biotech Clinical Summaries - Nutra

... Background: Symptoms of irritable bowel syndrome (IBS) can have a profound impact on emotional health and quality of life, and current treatments are sometimes unsatisfactory for patients facing this lifelong disease. Probiotics, which can normalize gastrointestinal microflora, may alleviate symptom ...
Aluminum Toxicity Is Associated with
Aluminum Toxicity Is Associated with

... Potential mechanisms of Al toxicity measured as Al-induced inhibition of growth in cultured tobacco cells (Nicotiana tabacum, nonchlorophyllic cell line SL) and pea (Pisum sativum) roots were investigated. Compared with the control treatment without Al, the accumulation of Al in tobacco cells caused ...
Mechanism of efficient transfection of the nasal airway
Mechanism of efficient transfection of the nasal airway

... Effect of the preperfusion of deionized water on transfection of the nasal airway epithelium with pDNA We then sequentially perfused mice with deionized water for different periods of time, followed by pDNA in deionized water. There was a time-dependent decrease of the luciferase activity. The admin ...
Physiology Study Guide
Physiology Study Guide

... Explain the following: twitch, summation, tetanus, fatigue. What factors affect the strength of contraction? How are different nutrients used by skeletal muscle and why? How is movement controlled and coordinated? Explain the purposes and expected results for each of the muscle physiology tests we d ...
A Microbial Avenue to Cell Cycle Control in the Plant
A Microbial Avenue to Cell Cycle Control in the Plant

... UV-mutagenized cells were deposited on agar to form colonies and picked robotically into 384-well plates. After replica pinning, ts mutants were identified on the 33°C plate (black arrowheads) based on reduction of biomass compared with 21°C. All ts mutants were screened by time-lapse microscopy to i ...
Identification and localization of a β‐COP‐like protein involved in the
Identification and localization of a β‐COP‐like protein involved in the

... cells, the GA is characterized by more than a hundred Golgi stacks dispersed throughout the cytoplasm (SatiatJeunemaitre and Hawes, 1992). These stacks consist of membrane-bounded cisternae, organized in a polarized manner and surrounded by populations of vesicles (Robinson et al., 1998; Dupree and ...
Mutational analysis supports a core role for Drosophila a
Mutational analysis supports a core role for Drosophila a

... GE30561 is a homozygous viable insertion that did not express its white (w+) marker. However, mobilization of the P element generated offspring that expressed w+, suggesting that the insertion of the P element at a different genomic location had occurred. We concluded that the expression of w+ at th ...
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Organ-on-a-chip

An organ-on-a-chip (OC) is a multi-channel 3-D microfluidic cell culture chip that simulates the activities, mechanics and physiological response of entire organs and organ systems. It constitutes the subject matter of significant biomedical engineering research, more precisely in bio-MEMS. The convergence of labs-on-chips (LOCs) and cell biology has permitted the study of human physiology in an organ-specific context, introducing a novel model of in vitro multicellular human organisms. One day, they will perhaps abolish the need for animals in drug development and toxin testing.Although multiple publications claim to have translated organ functions onto this interface, the movement towards this microfluidic application is still in its infancy. Organs-on-chips will vary in design and approach between different researchers. As such, validation and optimization of these systems will likely be a long process. Organs that have been simulated by microfluidic devices include the heart, the lung, kidney, artery, bone, cartilage, skin and more.Nevertheless, building valid artificial organs requires not only a precise cellular manipulation, but a detailed understanding of the human body’s fundamental intricate response to any event. A common concern with organs-on-chips lies in the isolation of organs during testing. ""If you don’t use as close to the total physiological system that you can, you’re likely to run into troubles"" says William Haseltine, founder of Rockville, Maryland. Microfabrication, microelectronics and microfluidics offer the prospect of modeling sophisticated in vitro physiological responses under accurately simulated conditions.
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